Nanoarchitectures based on multi-walled carbon nanotubes non-covalently functionalized with Concanavalin A: A new building-block with supramolecular recognition properties for the...
- Autores
- Ortiz, Elvis; Gallay, Pablo; Galicia, Laura; Eguílaz Rubio, Marcos; Rivas, Gustavo Adolfo
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- We propose an innovative nanoarchitecture for the development of electrochemical biosensors based on the non-covalent functionalization of multi-walled carbon nanotubes (MWCNTs) with the lectin Concanavalin A (ConA) and the site-specific supramolecular binding of glycobiomolecules. As proof-of-concept, we propose the use of two glycoenzymes, glucose oxidase (GOx) and horseradish peroxidase (HRP), for building mono and bienzymatic glucose biosensors. The selected conditions for the preparation of the dispersion were 1.5 mg MWCNTs in 1.0 mL of 2.0 mg mL −1 ConA sonicated for 5.0 min with sonicator probe. The monoenzymatic glucose biosensor was prepared by casting GCE with the MWCNTs-ConA dispersion (GCE/MWCNTs-ConA) followed by the interaction with GOx (GCE/MWCNTs-ConA/GOx), while the bienzymatic one was obtained by interaction of GCE/MWCNTs-ConA with GOx + HRP (GCE/MWCNTs-ConA/GOx-HRP). The best analytical performance was obtained with the bienzymatic biosensor from the amperometric response at -0.050 V in the presence of 1.0 × 10 -4 M hydroquinone. The sensitivity was (2.22 ± 0.03) μA mM −1 (which was 5.2 times higher than the one obtained with the monoenzymatic biosensor) and a detection limit of 0.31 μM. The reproducibility was 5.4% and the biosensor was challenged with human blood serum showing an excellent correlation with the values reported by the laboratory.
Fil: Ortiz, Elvis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina. Universidad Autónoma Metropolitana Iztapalapa; México
Fil: Gallay, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Galicia, Laura. Universidad Autónoma Metropolitana Iztapalapa; México
Fil: Eguílaz Rubio, Marcos. Universidad Autónoma Metropolitana Iztapalapa; México
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina - Materia
-
MULTI-WALLED CARBON NANOTUBES FUNCTIONALIZATION
CONCANAVALIN A
GLUCOSE ELECTROCHEMICAL BIOSENSOR
BIENZYMATIC BIOSENSOR
SITE-SPECIFIC SUPRAMOLECULAR BINDING - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/125566
Ver los metadatos del registro completo
id |
CONICETDig_d8f1065ad425fc32f730659a3d084bc6 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/125566 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Nanoarchitectures based on multi-walled carbon nanotubes non-covalently functionalized with Concanavalin A: A new building-block with supramolecular recognition properties for the development of electrochemical biosensorsOrtiz, ElvisGallay, PabloGalicia, LauraEguílaz Rubio, MarcosRivas, Gustavo AdolfoMULTI-WALLED CARBON NANOTUBES FUNCTIONALIZATIONCONCANAVALIN AGLUCOSE ELECTROCHEMICAL BIOSENSORBIENZYMATIC BIOSENSORSITE-SPECIFIC SUPRAMOLECULAR BINDINGhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2We propose an innovative nanoarchitecture for the development of electrochemical biosensors based on the non-covalent functionalization of multi-walled carbon nanotubes (MWCNTs) with the lectin Concanavalin A (ConA) and the site-specific supramolecular binding of glycobiomolecules. As proof-of-concept, we propose the use of two glycoenzymes, glucose oxidase (GOx) and horseradish peroxidase (HRP), for building mono and bienzymatic glucose biosensors. The selected conditions for the preparation of the dispersion were 1.5 mg MWCNTs in 1.0 mL of 2.0 mg mL −1 ConA sonicated for 5.0 min with sonicator probe. The monoenzymatic glucose biosensor was prepared by casting GCE with the MWCNTs-ConA dispersion (GCE/MWCNTs-ConA) followed by the interaction with GOx (GCE/MWCNTs-ConA/GOx), while the bienzymatic one was obtained by interaction of GCE/MWCNTs-ConA with GOx + HRP (GCE/MWCNTs-ConA/GOx-HRP). The best analytical performance was obtained with the bienzymatic biosensor from the amperometric response at -0.050 V in the presence of 1.0 × 10 -4 M hydroquinone. The sensitivity was (2.22 ± 0.03) μA mM −1 (which was 5.2 times higher than the one obtained with the monoenzymatic biosensor) and a detection limit of 0.31 μM. The reproducibility was 5.4% and the biosensor was challenged with human blood serum showing an excellent correlation with the values reported by the laboratory.Fil: Ortiz, Elvis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina. Universidad Autónoma Metropolitana Iztapalapa; MéxicoFil: Gallay, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Galicia, Laura. Universidad Autónoma Metropolitana Iztapalapa; MéxicoFil: Eguílaz Rubio, Marcos. Universidad Autónoma Metropolitana Iztapalapa; MéxicoFil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaElsevier Science SA2019-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/125566Ortiz, Elvis; Gallay, Pablo; Galicia, Laura; Eguílaz Rubio, Marcos; Rivas, Gustavo Adolfo; Nanoarchitectures based on multi-walled carbon nanotubes non-covalently functionalized with Concanavalin A: A new building-block with supramolecular recognition properties for the development of electrochemical biosensors; Elsevier Science SA; Sensors and Actuators B: Chemical; 292; 8-2019; 254-2620925-4005CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0925400519306471info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2019.04.114info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:22Zoai:ri.conicet.gov.ar:11336/125566instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:22.616CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Nanoarchitectures based on multi-walled carbon nanotubes non-covalently functionalized with Concanavalin A: A new building-block with supramolecular recognition properties for the development of electrochemical biosensors |
title |
Nanoarchitectures based on multi-walled carbon nanotubes non-covalently functionalized with Concanavalin A: A new building-block with supramolecular recognition properties for the development of electrochemical biosensors |
spellingShingle |
Nanoarchitectures based on multi-walled carbon nanotubes non-covalently functionalized with Concanavalin A: A new building-block with supramolecular recognition properties for the development of electrochemical biosensors Ortiz, Elvis MULTI-WALLED CARBON NANOTUBES FUNCTIONALIZATION CONCANAVALIN A GLUCOSE ELECTROCHEMICAL BIOSENSOR BIENZYMATIC BIOSENSOR SITE-SPECIFIC SUPRAMOLECULAR BINDING |
title_short |
Nanoarchitectures based on multi-walled carbon nanotubes non-covalently functionalized with Concanavalin A: A new building-block with supramolecular recognition properties for the development of electrochemical biosensors |
title_full |
Nanoarchitectures based on multi-walled carbon nanotubes non-covalently functionalized with Concanavalin A: A new building-block with supramolecular recognition properties for the development of electrochemical biosensors |
title_fullStr |
Nanoarchitectures based on multi-walled carbon nanotubes non-covalently functionalized with Concanavalin A: A new building-block with supramolecular recognition properties for the development of electrochemical biosensors |
title_full_unstemmed |
Nanoarchitectures based on multi-walled carbon nanotubes non-covalently functionalized with Concanavalin A: A new building-block with supramolecular recognition properties for the development of electrochemical biosensors |
title_sort |
Nanoarchitectures based on multi-walled carbon nanotubes non-covalently functionalized with Concanavalin A: A new building-block with supramolecular recognition properties for the development of electrochemical biosensors |
dc.creator.none.fl_str_mv |
Ortiz, Elvis Gallay, Pablo Galicia, Laura Eguílaz Rubio, Marcos Rivas, Gustavo Adolfo |
author |
Ortiz, Elvis |
author_facet |
Ortiz, Elvis Gallay, Pablo Galicia, Laura Eguílaz Rubio, Marcos Rivas, Gustavo Adolfo |
author_role |
author |
author2 |
Gallay, Pablo Galicia, Laura Eguílaz Rubio, Marcos Rivas, Gustavo Adolfo |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
MULTI-WALLED CARBON NANOTUBES FUNCTIONALIZATION CONCANAVALIN A GLUCOSE ELECTROCHEMICAL BIOSENSOR BIENZYMATIC BIOSENSOR SITE-SPECIFIC SUPRAMOLECULAR BINDING |
topic |
MULTI-WALLED CARBON NANOTUBES FUNCTIONALIZATION CONCANAVALIN A GLUCOSE ELECTROCHEMICAL BIOSENSOR BIENZYMATIC BIOSENSOR SITE-SPECIFIC SUPRAMOLECULAR BINDING |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/2.10 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
We propose an innovative nanoarchitecture for the development of electrochemical biosensors based on the non-covalent functionalization of multi-walled carbon nanotubes (MWCNTs) with the lectin Concanavalin A (ConA) and the site-specific supramolecular binding of glycobiomolecules. As proof-of-concept, we propose the use of two glycoenzymes, glucose oxidase (GOx) and horseradish peroxidase (HRP), for building mono and bienzymatic glucose biosensors. The selected conditions for the preparation of the dispersion were 1.5 mg MWCNTs in 1.0 mL of 2.0 mg mL −1 ConA sonicated for 5.0 min with sonicator probe. The monoenzymatic glucose biosensor was prepared by casting GCE with the MWCNTs-ConA dispersion (GCE/MWCNTs-ConA) followed by the interaction with GOx (GCE/MWCNTs-ConA/GOx), while the bienzymatic one was obtained by interaction of GCE/MWCNTs-ConA with GOx + HRP (GCE/MWCNTs-ConA/GOx-HRP). The best analytical performance was obtained with the bienzymatic biosensor from the amperometric response at -0.050 V in the presence of 1.0 × 10 -4 M hydroquinone. The sensitivity was (2.22 ± 0.03) μA mM −1 (which was 5.2 times higher than the one obtained with the monoenzymatic biosensor) and a detection limit of 0.31 μM. The reproducibility was 5.4% and the biosensor was challenged with human blood serum showing an excellent correlation with the values reported by the laboratory. Fil: Ortiz, Elvis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina. Universidad Autónoma Metropolitana Iztapalapa; México Fil: Gallay, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina Fil: Galicia, Laura. Universidad Autónoma Metropolitana Iztapalapa; México Fil: Eguílaz Rubio, Marcos. Universidad Autónoma Metropolitana Iztapalapa; México Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina |
description |
We propose an innovative nanoarchitecture for the development of electrochemical biosensors based on the non-covalent functionalization of multi-walled carbon nanotubes (MWCNTs) with the lectin Concanavalin A (ConA) and the site-specific supramolecular binding of glycobiomolecules. As proof-of-concept, we propose the use of two glycoenzymes, glucose oxidase (GOx) and horseradish peroxidase (HRP), for building mono and bienzymatic glucose biosensors. The selected conditions for the preparation of the dispersion were 1.5 mg MWCNTs in 1.0 mL of 2.0 mg mL −1 ConA sonicated for 5.0 min with sonicator probe. The monoenzymatic glucose biosensor was prepared by casting GCE with the MWCNTs-ConA dispersion (GCE/MWCNTs-ConA) followed by the interaction with GOx (GCE/MWCNTs-ConA/GOx), while the bienzymatic one was obtained by interaction of GCE/MWCNTs-ConA with GOx + HRP (GCE/MWCNTs-ConA/GOx-HRP). The best analytical performance was obtained with the bienzymatic biosensor from the amperometric response at -0.050 V in the presence of 1.0 × 10 -4 M hydroquinone. The sensitivity was (2.22 ± 0.03) μA mM −1 (which was 5.2 times higher than the one obtained with the monoenzymatic biosensor) and a detection limit of 0.31 μM. The reproducibility was 5.4% and the biosensor was challenged with human blood serum showing an excellent correlation with the values reported by the laboratory. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/125566 Ortiz, Elvis; Gallay, Pablo; Galicia, Laura; Eguílaz Rubio, Marcos; Rivas, Gustavo Adolfo; Nanoarchitectures based on multi-walled carbon nanotubes non-covalently functionalized with Concanavalin A: A new building-block with supramolecular recognition properties for the development of electrochemical biosensors; Elsevier Science SA; Sensors and Actuators B: Chemical; 292; 8-2019; 254-262 0925-4005 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/125566 |
identifier_str_mv |
Ortiz, Elvis; Gallay, Pablo; Galicia, Laura; Eguílaz Rubio, Marcos; Rivas, Gustavo Adolfo; Nanoarchitectures based on multi-walled carbon nanotubes non-covalently functionalized with Concanavalin A: A new building-block with supramolecular recognition properties for the development of electrochemical biosensors; Elsevier Science SA; Sensors and Actuators B: Chemical; 292; 8-2019; 254-262 0925-4005 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0925400519306471 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2019.04.114 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science SA |
publisher.none.fl_str_mv |
Elsevier Science SA |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613807216787456 |
score |
13.070432 |