Estrogen and progesterone modulation of eosinophilic infiltration of the rat uterine cervix

Autores
Ramos, Jorge Guillermo; Varayoud, Jorgelina Guadalupe; Kass, Laura; Rodriguez, Horacio Adolfo; Muñoz de Toro, Monica Milagros; Montes, Gregorio S.; Luque, Enrique Hugo
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ripening of the rat cervix involves widespread collagenolysis that follows an eosinophilic leukocyte infiltration. The hormonal control of these events is not well understood. The aims of this study were to investigate the mechanism through which progesterone (P) and 17β-estradiol (E2) modulate eosinophilic invasion and to determine if this event is protein synthesis mediated. Cervical eosinophilic invasion was measured in intact rats during the second half of pregnancy and compared with values from ovariectomized (O) pseudopregnant (PSP) rats treated with P and E2 in doses that mimicked the levels of pregnancy. Other O-PSP rats were treated with an E2 antagonist (tamoxifen) and the antiprogestin RU-486. To study the role of protein synthesis in eosinophilic invasion of the cervix, rats were treated with actinomycin-D (an inhibitor of mRNA synthesis), and animals were sacrificed on D21 or D22 to evaluate eosinophilic invasion. Rats treated with E2 showed high levels of infiltration and tamoxifen blocked this E2 effect. On the other hand, P antagonized the stimulatory effects of E2 on eosinophilic invasion, however when the P and E2 treated rats were injected with RU-486 the inhibitory effect of P was reversed. In intact pregnant rats a sharp rise in eosinophilic infiltration was detected on D23, 20 h after the fall of serum P. Finally, E2 treated rats injected with actinomycin-D had no invasion of eosinophils. In conclusion, the estrogen-triggered eosinophil invasion is affected by the classic estrogen receptor antagonist tamoxifen and by the mRNA synthesis blocker actinomycin-D suggesting a genomic action of E2. Furthermore, the estrogen effect is blocked by P and this inhibition is reversed by RU-486. Copyright (C) 2000 Elsevier Science Inc.
Fil: Ramos, Jorge Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Varayoud, Jorgelina Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Kass, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Rodriguez, Horacio Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Muñoz de Toro, Monica Milagros. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Montes, Gregorio S.. The University of São Paulo School of Medicine; Brasil
Fil: Luque, Enrique Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Materia
Cervix
Eosinophils
Estrogen
Parturition
Progesterone
Ru-486
Tamoxifen
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/72044
Acceder
id CONICETDig_c9b062d4c0f67a6fa5834004d934d8e4
oai_identifier_str oai:ri.conicet.gov.ar:11336/72044
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Estrogen and progesterone modulation of eosinophilic infiltration of the rat uterine cervixRamos, Jorge GuillermoVarayoud, Jorgelina GuadalupeKass, LauraRodriguez, Horacio AdolfoMuñoz de Toro, Monica MilagrosMontes, Gregorio S.Luque, Enrique HugoCervixEosinophilsEstrogenParturitionProgesteroneRu-486Tamoxifenhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Ripening of the rat cervix involves widespread collagenolysis that follows an eosinophilic leukocyte infiltration. The hormonal control of these events is not well understood. The aims of this study were to investigate the mechanism through which progesterone (P) and 17β-estradiol (E2) modulate eosinophilic invasion and to determine if this event is protein synthesis mediated. Cervical eosinophilic invasion was measured in intact rats during the second half of pregnancy and compared with values from ovariectomized (O) pseudopregnant (PSP) rats treated with P and E2 in doses that mimicked the levels of pregnancy. Other O-PSP rats were treated with an E2 antagonist (tamoxifen) and the antiprogestin RU-486. To study the role of protein synthesis in eosinophilic invasion of the cervix, rats were treated with actinomycin-D (an inhibitor of mRNA synthesis), and animals were sacrificed on D21 or D22 to evaluate eosinophilic invasion. Rats treated with E2 showed high levels of infiltration and tamoxifen blocked this E2 effect. On the other hand, P antagonized the stimulatory effects of E2 on eosinophilic invasion, however when the P and E2 treated rats were injected with RU-486 the inhibitory effect of P was reversed. In intact pregnant rats a sharp rise in eosinophilic infiltration was detected on D23, 20 h after the fall of serum P. Finally, E2 treated rats injected with actinomycin-D had no invasion of eosinophils. In conclusion, the estrogen-triggered eosinophil invasion is affected by the classic estrogen receptor antagonist tamoxifen and by the mRNA synthesis blocker actinomycin-D suggesting a genomic action of E2. Furthermore, the estrogen effect is blocked by P and this inhibition is reversed by RU-486. Copyright (C) 2000 Elsevier Science Inc.Fil: Ramos, Jorge Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Varayoud, Jorgelina Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Kass, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Rodriguez, Horacio Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Muñoz de Toro, Monica Milagros. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Montes, Gregorio S.. The University of São Paulo School of Medicine; BrasilFil: Luque, Enrique Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaElsevier Science Inc2000-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/72044Ramos, Jorge Guillermo; Varayoud, Jorgelina Guadalupe; Kass, Laura; Rodriguez, Horacio Adolfo; Muñoz de Toro, Monica Milagros; et al.; Estrogen and progesterone modulation of eosinophilic infiltration of the rat uterine cervix; Elsevier Science Inc; Steroids; 65; 7; 7-2000; 409-4140039-128XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0039128X00000982info:eu-repo/semantics/altIdentifier/doi/10.1016/S0039-128X(00)00098-2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:48Zoai:ri.conicet.gov.ar:11336/72044instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:48.712CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Estrogen and progesterone modulation of eosinophilic infiltration of the rat uterine cervix
title Estrogen and progesterone modulation of eosinophilic infiltration of the rat uterine cervix
spellingShingle Estrogen and progesterone modulation of eosinophilic infiltration of the rat uterine cervix
Ramos, Jorge Guillermo
Cervix
Eosinophils
Estrogen
Parturition
Progesterone
Ru-486
Tamoxifen
title_short Estrogen and progesterone modulation of eosinophilic infiltration of the rat uterine cervix
title_full Estrogen and progesterone modulation of eosinophilic infiltration of the rat uterine cervix
title_fullStr Estrogen and progesterone modulation of eosinophilic infiltration of the rat uterine cervix
title_full_unstemmed Estrogen and progesterone modulation of eosinophilic infiltration of the rat uterine cervix
title_sort Estrogen and progesterone modulation of eosinophilic infiltration of the rat uterine cervix
dc.creator.none.fl_str_mv Ramos, Jorge Guillermo
Varayoud, Jorgelina Guadalupe
Kass, Laura
Rodriguez, Horacio Adolfo
Muñoz de Toro, Monica Milagros
Montes, Gregorio S.
Luque, Enrique Hugo
author Ramos, Jorge Guillermo
author_facet Ramos, Jorge Guillermo
Varayoud, Jorgelina Guadalupe
Kass, Laura
Rodriguez, Horacio Adolfo
Muñoz de Toro, Monica Milagros
Montes, Gregorio S.
Luque, Enrique Hugo
author_role author
author2 Varayoud, Jorgelina Guadalupe
Kass, Laura
Rodriguez, Horacio Adolfo
Muñoz de Toro, Monica Milagros
Montes, Gregorio S.
Luque, Enrique Hugo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Cervix
Eosinophils
Estrogen
Parturition
Progesterone
Ru-486
Tamoxifen
topic Cervix
Eosinophils
Estrogen
Parturition
Progesterone
Ru-486
Tamoxifen
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Ripening of the rat cervix involves widespread collagenolysis that follows an eosinophilic leukocyte infiltration. The hormonal control of these events is not well understood. The aims of this study were to investigate the mechanism through which progesterone (P) and 17β-estradiol (E2) modulate eosinophilic invasion and to determine if this event is protein synthesis mediated. Cervical eosinophilic invasion was measured in intact rats during the second half of pregnancy and compared with values from ovariectomized (O) pseudopregnant (PSP) rats treated with P and E2 in doses that mimicked the levels of pregnancy. Other O-PSP rats were treated with an E2 antagonist (tamoxifen) and the antiprogestin RU-486. To study the role of protein synthesis in eosinophilic invasion of the cervix, rats were treated with actinomycin-D (an inhibitor of mRNA synthesis), and animals were sacrificed on D21 or D22 to evaluate eosinophilic invasion. Rats treated with E2 showed high levels of infiltration and tamoxifen blocked this E2 effect. On the other hand, P antagonized the stimulatory effects of E2 on eosinophilic invasion, however when the P and E2 treated rats were injected with RU-486 the inhibitory effect of P was reversed. In intact pregnant rats a sharp rise in eosinophilic infiltration was detected on D23, 20 h after the fall of serum P. Finally, E2 treated rats injected with actinomycin-D had no invasion of eosinophils. In conclusion, the estrogen-triggered eosinophil invasion is affected by the classic estrogen receptor antagonist tamoxifen and by the mRNA synthesis blocker actinomycin-D suggesting a genomic action of E2. Furthermore, the estrogen effect is blocked by P and this inhibition is reversed by RU-486. Copyright (C) 2000 Elsevier Science Inc.
Fil: Ramos, Jorge Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Varayoud, Jorgelina Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Kass, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Rodriguez, Horacio Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Muñoz de Toro, Monica Milagros. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Montes, Gregorio S.. The University of São Paulo School of Medicine; Brasil
Fil: Luque, Enrique Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
description Ripening of the rat cervix involves widespread collagenolysis that follows an eosinophilic leukocyte infiltration. The hormonal control of these events is not well understood. The aims of this study were to investigate the mechanism through which progesterone (P) and 17β-estradiol (E2) modulate eosinophilic invasion and to determine if this event is protein synthesis mediated. Cervical eosinophilic invasion was measured in intact rats during the second half of pregnancy and compared with values from ovariectomized (O) pseudopregnant (PSP) rats treated with P and E2 in doses that mimicked the levels of pregnancy. Other O-PSP rats were treated with an E2 antagonist (tamoxifen) and the antiprogestin RU-486. To study the role of protein synthesis in eosinophilic invasion of the cervix, rats were treated with actinomycin-D (an inhibitor of mRNA synthesis), and animals were sacrificed on D21 or D22 to evaluate eosinophilic invasion. Rats treated with E2 showed high levels of infiltration and tamoxifen blocked this E2 effect. On the other hand, P antagonized the stimulatory effects of E2 on eosinophilic invasion, however when the P and E2 treated rats were injected with RU-486 the inhibitory effect of P was reversed. In intact pregnant rats a sharp rise in eosinophilic infiltration was detected on D23, 20 h after the fall of serum P. Finally, E2 treated rats injected with actinomycin-D had no invasion of eosinophils. In conclusion, the estrogen-triggered eosinophil invasion is affected by the classic estrogen receptor antagonist tamoxifen and by the mRNA synthesis blocker actinomycin-D suggesting a genomic action of E2. Furthermore, the estrogen effect is blocked by P and this inhibition is reversed by RU-486. Copyright (C) 2000 Elsevier Science Inc.
publishDate 2000
dc.date.none.fl_str_mv 2000-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/72044
Ramos, Jorge Guillermo; Varayoud, Jorgelina Guadalupe; Kass, Laura; Rodriguez, Horacio Adolfo; Muñoz de Toro, Monica Milagros; et al.; Estrogen and progesterone modulation of eosinophilic infiltration of the rat uterine cervix; Elsevier Science Inc; Steroids; 65; 7; 7-2000; 409-414
0039-128X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/72044
identifier_str_mv Ramos, Jorge Guillermo; Varayoud, Jorgelina Guadalupe; Kass, Laura; Rodriguez, Horacio Adolfo; Muñoz de Toro, Monica Milagros; et al.; Estrogen and progesterone modulation of eosinophilic infiltration of the rat uterine cervix; Elsevier Science Inc; Steroids; 65; 7; 7-2000; 409-414
0039-128X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0039128X00000982
info:eu-repo/semantics/altIdentifier/doi/10.1016/S0039-128X(00)00098-2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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