Altered expression of aromatase, estrogen receptors and progesterone receptors in human leydig cell hyperplasia

Autores
Gonzalez, Candela Rocio; Inserra, Pablo Ignacio Felipe; Terradas, Claudio; Ponzio, Roberto Oscar; Puigdomenech, Elisa; Levalle, Oscar; Vitullo, Alfredo Daniel; Calandra, Ricardo Saul; Gonzalez de Calvar, Silvia Ines
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Testicular function is regulated by pituitary hormones and also by paracrine and autocrine factors. A number of reports have pointed out the importance of estrogens and progesterone in male reproductive tract. Recently, we have reported in testicular biopsies from men with Sertoli Cell Only Syndrome (SCO) or Hypospermatogenesis (H) with Leydig cell hyperplasia (LCH) an increase in the expression of the TGFB1 and its receptors ALK1 and endoglin, which are involved in the proliferation of Leydig cells. The aim of the present work was to analyze the expression of aromatase, estrogen and progesterone receptors (ERs, PR) in pathological testicular biopsies with SCO or H with and without LCH. The ERs and CYP19 proteins were detected in the Leydig cells from all pathological biopsies analyzed. Biopsies with SCO or H with LCH showed an increment in the immunostaining of CYP19 and ERs in the Leydig cells respect to biopsies without LCH. The gene expression of CYP19 was increased in SCO or H biopsies with LCH respect to SCO and H biopsies without LCH. PR was localized in Leydig cells and showed a significant increment in biopsies with LCH respect from biopsies without LCH. The gene expression of both PRA and PRB was increased in biopsies with LCH respect to biopsies without LCH. In concussion, alterations in the gene expression of aromatase, ERs, and PR and the likely interactions of these systems with locally produced factors such as growth factors and cytokines, might lead to Leydig cell proliferation in testicular pathology.
Fil: Gonzalez, Candela Rocio. Universidad Maimónides. Area de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Inserra, Pablo Ignacio Felipe. Universidad Maimónides. Area de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Terradas, Claudio. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Agudos Carlos Durand; Argentina
Fil: Ponzio, Roberto Oscar. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Puigdomenech, Elisa. No especifíca;
Fil: Levalle, Oscar. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Carlos G. Durand"; Argentina
Fil: Vitullo, Alfredo Daniel. Universidad Maimónides. Area de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Calandra, Ricardo Saul. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Gonzalez de Calvar, Silvia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Materia
Aromatase
Estrogen
Progesterone
Leydig
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7738
Acceder
id CONICETDig_792efec25b223b1d810394c2640b43f5
oai_identifier_str oai:ri.conicet.gov.ar:11336/7738
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Altered expression of aromatase, estrogen receptors and progesterone receptors in human leydig cell hyperplasiaGonzalez, Candela RocioInserra, Pablo Ignacio FelipeTerradas, ClaudioPonzio, Roberto OscarPuigdomenech, ElisaLevalle, OscarVitullo, Alfredo DanielCalandra, Ricardo SaulGonzalez de Calvar, Silvia InesAromataseEstrogenProgesteroneLeydighttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Testicular function is regulated by pituitary hormones and also by paracrine and autocrine factors. A number of reports have pointed out the importance of estrogens and progesterone in male reproductive tract. Recently, we have reported in testicular biopsies from men with Sertoli Cell Only Syndrome (SCO) or Hypospermatogenesis (H) with Leydig cell hyperplasia (LCH) an increase in the expression of the TGFB1 and its receptors ALK1 and endoglin, which are involved in the proliferation of Leydig cells. The aim of the present work was to analyze the expression of aromatase, estrogen and progesterone receptors (ERs, PR) in pathological testicular biopsies with SCO or H with and without LCH. The ERs and CYP19 proteins were detected in the Leydig cells from all pathological biopsies analyzed. Biopsies with SCO or H with LCH showed an increment in the immunostaining of CYP19 and ERs in the Leydig cells respect to biopsies without LCH. The gene expression of CYP19 was increased in SCO or H biopsies with LCH respect to SCO and H biopsies without LCH. PR was localized in Leydig cells and showed a significant increment in biopsies with LCH respect from biopsies without LCH. The gene expression of both PRA and PRB was increased in biopsies with LCH respect to biopsies without LCH. In concussion, alterations in the gene expression of aromatase, ERs, and PR and the likely interactions of these systems with locally produced factors such as growth factors and cytokines, might lead to Leydig cell proliferation in testicular pathology.Fil: Gonzalez, Candela Rocio. Universidad Maimónides. Area de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Inserra, Pablo Ignacio Felipe. Universidad Maimónides. Area de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Terradas, Claudio. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Agudos Carlos Durand; ArgentinaFil: Ponzio, Roberto Oscar. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Puigdomenech, Elisa. No especifíca;Fil: Levalle, Oscar. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Carlos G. Durand"; ArgentinaFil: Vitullo, Alfredo Daniel. Universidad Maimónides. Area de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Calandra, Ricardo Saul. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Gonzalez de Calvar, Silvia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaScientific Research2015-01-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7738Gonzalez, Candela Rocio; Inserra, Pablo Ignacio Felipe; Terradas, Claudio; Ponzio, Roberto Oscar; Puigdomenech, Elisa; et al.; Altered expression of aromatase, estrogen receptors and progesterone receptors in human leydig cell hyperplasia; Scientific Research; Advances in Bioscience and Biotechnology; 6; 1; 31-1-2015; 1-102156-8456enginfo:eu-repo/semantics/altIdentifier/doi/10.4236/abb.2015.61001info:eu-repo/semantics/altIdentifier/url/http://www.scirp.org/journal/PaperInformation.aspx?PaperID=53101info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:42Zoai:ri.conicet.gov.ar:11336/7738instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:42.948CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Altered expression of aromatase, estrogen receptors and progesterone receptors in human leydig cell hyperplasia
title Altered expression of aromatase, estrogen receptors and progesterone receptors in human leydig cell hyperplasia
spellingShingle Altered expression of aromatase, estrogen receptors and progesterone receptors in human leydig cell hyperplasia
Gonzalez, Candela Rocio
Aromatase
Estrogen
Progesterone
Leydig
title_short Altered expression of aromatase, estrogen receptors and progesterone receptors in human leydig cell hyperplasia
title_full Altered expression of aromatase, estrogen receptors and progesterone receptors in human leydig cell hyperplasia
title_fullStr Altered expression of aromatase, estrogen receptors and progesterone receptors in human leydig cell hyperplasia
title_full_unstemmed Altered expression of aromatase, estrogen receptors and progesterone receptors in human leydig cell hyperplasia
title_sort Altered expression of aromatase, estrogen receptors and progesterone receptors in human leydig cell hyperplasia
dc.creator.none.fl_str_mv Gonzalez, Candela Rocio
Inserra, Pablo Ignacio Felipe
Terradas, Claudio
Ponzio, Roberto Oscar
Puigdomenech, Elisa
Levalle, Oscar
Vitullo, Alfredo Daniel
Calandra, Ricardo Saul
Gonzalez de Calvar, Silvia Ines
author Gonzalez, Candela Rocio
author_facet Gonzalez, Candela Rocio
Inserra, Pablo Ignacio Felipe
Terradas, Claudio
Ponzio, Roberto Oscar
Puigdomenech, Elisa
Levalle, Oscar
Vitullo, Alfredo Daniel
Calandra, Ricardo Saul
Gonzalez de Calvar, Silvia Ines
author_role author
author2 Inserra, Pablo Ignacio Felipe
Terradas, Claudio
Ponzio, Roberto Oscar
Puigdomenech, Elisa
Levalle, Oscar
Vitullo, Alfredo Daniel
Calandra, Ricardo Saul
Gonzalez de Calvar, Silvia Ines
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Aromatase
Estrogen
Progesterone
Leydig
topic Aromatase
Estrogen
Progesterone
Leydig
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Testicular function is regulated by pituitary hormones and also by paracrine and autocrine factors. A number of reports have pointed out the importance of estrogens and progesterone in male reproductive tract. Recently, we have reported in testicular biopsies from men with Sertoli Cell Only Syndrome (SCO) or Hypospermatogenesis (H) with Leydig cell hyperplasia (LCH) an increase in the expression of the TGFB1 and its receptors ALK1 and endoglin, which are involved in the proliferation of Leydig cells. The aim of the present work was to analyze the expression of aromatase, estrogen and progesterone receptors (ERs, PR) in pathological testicular biopsies with SCO or H with and without LCH. The ERs and CYP19 proteins were detected in the Leydig cells from all pathological biopsies analyzed. Biopsies with SCO or H with LCH showed an increment in the immunostaining of CYP19 and ERs in the Leydig cells respect to biopsies without LCH. The gene expression of CYP19 was increased in SCO or H biopsies with LCH respect to SCO and H biopsies without LCH. PR was localized in Leydig cells and showed a significant increment in biopsies with LCH respect from biopsies without LCH. The gene expression of both PRA and PRB was increased in biopsies with LCH respect to biopsies without LCH. In concussion, alterations in the gene expression of aromatase, ERs, and PR and the likely interactions of these systems with locally produced factors such as growth factors and cytokines, might lead to Leydig cell proliferation in testicular pathology.
Fil: Gonzalez, Candela Rocio. Universidad Maimónides. Area de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Inserra, Pablo Ignacio Felipe. Universidad Maimónides. Area de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Terradas, Claudio. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Agudos Carlos Durand; Argentina
Fil: Ponzio, Roberto Oscar. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Puigdomenech, Elisa. No especifíca;
Fil: Levalle, Oscar. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Carlos G. Durand"; Argentina
Fil: Vitullo, Alfredo Daniel. Universidad Maimónides. Area de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Calandra, Ricardo Saul. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Gonzalez de Calvar, Silvia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
description Testicular function is regulated by pituitary hormones and also by paracrine and autocrine factors. A number of reports have pointed out the importance of estrogens and progesterone in male reproductive tract. Recently, we have reported in testicular biopsies from men with Sertoli Cell Only Syndrome (SCO) or Hypospermatogenesis (H) with Leydig cell hyperplasia (LCH) an increase in the expression of the TGFB1 and its receptors ALK1 and endoglin, which are involved in the proliferation of Leydig cells. The aim of the present work was to analyze the expression of aromatase, estrogen and progesterone receptors (ERs, PR) in pathological testicular biopsies with SCO or H with and without LCH. The ERs and CYP19 proteins were detected in the Leydig cells from all pathological biopsies analyzed. Biopsies with SCO or H with LCH showed an increment in the immunostaining of CYP19 and ERs in the Leydig cells respect to biopsies without LCH. The gene expression of CYP19 was increased in SCO or H biopsies with LCH respect to SCO and H biopsies without LCH. PR was localized in Leydig cells and showed a significant increment in biopsies with LCH respect from biopsies without LCH. The gene expression of both PRA and PRB was increased in biopsies with LCH respect to biopsies without LCH. In concussion, alterations in the gene expression of aromatase, ERs, and PR and the likely interactions of these systems with locally produced factors such as growth factors and cytokines, might lead to Leydig cell proliferation in testicular pathology.
publishDate 2015
dc.date.none.fl_str_mv 2015-01-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7738
Gonzalez, Candela Rocio; Inserra, Pablo Ignacio Felipe; Terradas, Claudio; Ponzio, Roberto Oscar; Puigdomenech, Elisa; et al.; Altered expression of aromatase, estrogen receptors and progesterone receptors in human leydig cell hyperplasia; Scientific Research; Advances in Bioscience and Biotechnology; 6; 1; 31-1-2015; 1-10
2156-8456
url http://hdl.handle.net/11336/7738
identifier_str_mv Gonzalez, Candela Rocio; Inserra, Pablo Ignacio Felipe; Terradas, Claudio; Ponzio, Roberto Oscar; Puigdomenech, Elisa; et al.; Altered expression of aromatase, estrogen receptors and progesterone receptors in human leydig cell hyperplasia; Scientific Research; Advances in Bioscience and Biotechnology; 6; 1; 31-1-2015; 1-10
2156-8456
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4236/abb.2015.61001
info:eu-repo/semantics/altIdentifier/url/http://www.scirp.org/journal/PaperInformation.aspx?PaperID=53101
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Scientific Research
publisher.none.fl_str_mv Scientific Research
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613485375258624
score 13.070432

Publicaciones similares