Serological Responses of Guinea Pigs and Heifers to Eight Different BoAHV-1 Vaccine Formulations

Autores
Camargo, Luana; Vieira Franklin, Yasmin; Resende da Silva, Gustavo Feliciano; Ferreira Santos, Janaína; Parreño, Gladys Viviana; Wigdorovitz, Andrés; Gomes, Viviani
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bovine alphaherpesvirus 1 (BoAHV-1) infection affects the production and reproductive performance of dairy and beef livestock, resulting in considerable economic losses. In addition to biosecurity measures, vaccination programs are effective strategies for controlling and preventing BoAHV-1 infection and transmission. We evaluated the serological immune response against BoAHV- 1 induced by eight different formulations of commercial vaccines: three modified live vaccines and five killed vaccines containing BoAHV type 1 or types 1 and 5. In the first experiment, 50 BoAHV- 1-seronegative guinea pigs were assigned to eight groups; each individual in the treatment groups received two doses (one-fifth of the bovine dose). The second experiment was conducted using 29 crossbred Holstein × Gir heifers in four groups of six to nine animals each. The serological immune response against BoAHV-1 was measured using virus neutralization and enzyme-linked immunosorbent assays to measure the total IgG against BoAHV. We evaluated the effects of the vaccine, time, and interaction of the vaccine and time on neutralizing antibodies against BoAHV-1. Killed vaccines produced low levels of antibodies against BoAHV-1, whereas modified live vaccines produced high levels of antibodies capable of providing neutralizing titers in the vaccinated animals, with the thermosensitive modified live vaccine showing the highest levels of antibodies.
Fil: Camargo, Luana. Universidade de Sao Paulo; Brasil
Fil: Vieira Franklin, Yasmin. Universidade de Sao Paulo; Brasil
Fil: Resende da Silva, Gustavo Feliciano. Universidade de Sao Paulo; Brasil
Fil: Ferreira Santos, Janaína. Universidade de Sao Paulo; Brasil
Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit.; Argentina
Fil: Wigdorovitz, Andrés. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit.; Argentina
Fil: Gomes, Viviani. Universidade de Sao Paulo; Brasil
Materia
BoHV-1
vaccine
Ab response
potency
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/260926
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/260926
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Serological Responses of Guinea Pigs and Heifers to Eight Different BoAHV-1 Vaccine FormulationsCamargo, LuanaVieira Franklin, YasminResende da Silva, Gustavo FelicianoFerreira Santos, JanaínaParreño, Gladys VivianaWigdorovitz, AndrésGomes, VivianiBoHV-1vaccineAb responsepotencyhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Bovine alphaherpesvirus 1 (BoAHV-1) infection affects the production and reproductive performance of dairy and beef livestock, resulting in considerable economic losses. In addition to biosecurity measures, vaccination programs are effective strategies for controlling and preventing BoAHV-1 infection and transmission. We evaluated the serological immune response against BoAHV- 1 induced by eight different formulations of commercial vaccines: three modified live vaccines and five killed vaccines containing BoAHV type 1 or types 1 and 5. In the first experiment, 50 BoAHV- 1-seronegative guinea pigs were assigned to eight groups; each individual in the treatment groups received two doses (one-fifth of the bovine dose). The second experiment was conducted using 29 crossbred Holstein × Gir heifers in four groups of six to nine animals each. The serological immune response against BoAHV-1 was measured using virus neutralization and enzyme-linked immunosorbent assays to measure the total IgG against BoAHV. We evaluated the effects of the vaccine, time, and interaction of the vaccine and time on neutralizing antibodies against BoAHV-1. Killed vaccines produced low levels of antibodies against BoAHV-1, whereas modified live vaccines produced high levels of antibodies capable of providing neutralizing titers in the vaccinated animals, with the thermosensitive modified live vaccine showing the highest levels of antibodies.Fil: Camargo, Luana. Universidade de Sao Paulo; BrasilFil: Vieira Franklin, Yasmin. Universidade de Sao Paulo; BrasilFil: Resende da Silva, Gustavo Feliciano. Universidade de Sao Paulo; BrasilFil: Ferreira Santos, Janaína. Universidade de Sao Paulo; BrasilFil: Parreño, Gladys Viviana. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit.; ArgentinaFil: Wigdorovitz, Andrés. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit.; ArgentinaFil: Gomes, Viviani. Universidade de Sao Paulo; BrasilMDPI2024-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/260926Camargo, Luana; Vieira Franklin, Yasmin; Resende da Silva, Gustavo Feliciano; Ferreira Santos, Janaína; Parreño, Gladys Viviana; et al.; Serological Responses of Guinea Pigs and Heifers to Eight Different BoAHV-1 Vaccine Formulations; MDPI; Vaccines; 12; 6; 6-2024; 1-132076-393XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-393X/12/6/615info:eu-repo/semantics/altIdentifier/doi/10.3390/vaccines12060615info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:59:37Zoai:ri.conicet.gov.ar:11336/260926instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:59:38.178CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Serological Responses of Guinea Pigs and Heifers to Eight Different BoAHV-1 Vaccine Formulations
title Serological Responses of Guinea Pigs and Heifers to Eight Different BoAHV-1 Vaccine Formulations
spellingShingle Serological Responses of Guinea Pigs and Heifers to Eight Different BoAHV-1 Vaccine Formulations
Camargo, Luana
BoHV-1
vaccine
Ab response
potency
title_short Serological Responses of Guinea Pigs and Heifers to Eight Different BoAHV-1 Vaccine Formulations
title_full Serological Responses of Guinea Pigs and Heifers to Eight Different BoAHV-1 Vaccine Formulations
title_fullStr Serological Responses of Guinea Pigs and Heifers to Eight Different BoAHV-1 Vaccine Formulations
title_full_unstemmed Serological Responses of Guinea Pigs and Heifers to Eight Different BoAHV-1 Vaccine Formulations
title_sort Serological Responses of Guinea Pigs and Heifers to Eight Different BoAHV-1 Vaccine Formulations
dc.creator.none.fl_str_mv Camargo, Luana
Vieira Franklin, Yasmin
Resende da Silva, Gustavo Feliciano
Ferreira Santos, Janaína
Parreño, Gladys Viviana
Wigdorovitz, Andrés
Gomes, Viviani
author Camargo, Luana
author_facet Camargo, Luana
Vieira Franklin, Yasmin
Resende da Silva, Gustavo Feliciano
Ferreira Santos, Janaína
Parreño, Gladys Viviana
Wigdorovitz, Andrés
Gomes, Viviani
author_role author
author2 Vieira Franklin, Yasmin
Resende da Silva, Gustavo Feliciano
Ferreira Santos, Janaína
Parreño, Gladys Viviana
Wigdorovitz, Andrés
Gomes, Viviani
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BoHV-1
vaccine
Ab response
potency
topic BoHV-1
vaccine
Ab response
potency
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Bovine alphaherpesvirus 1 (BoAHV-1) infection affects the production and reproductive performance of dairy and beef livestock, resulting in considerable economic losses. In addition to biosecurity measures, vaccination programs are effective strategies for controlling and preventing BoAHV-1 infection and transmission. We evaluated the serological immune response against BoAHV- 1 induced by eight different formulations of commercial vaccines: three modified live vaccines and five killed vaccines containing BoAHV type 1 or types 1 and 5. In the first experiment, 50 BoAHV- 1-seronegative guinea pigs were assigned to eight groups; each individual in the treatment groups received two doses (one-fifth of the bovine dose). The second experiment was conducted using 29 crossbred Holstein × Gir heifers in four groups of six to nine animals each. The serological immune response against BoAHV-1 was measured using virus neutralization and enzyme-linked immunosorbent assays to measure the total IgG against BoAHV. We evaluated the effects of the vaccine, time, and interaction of the vaccine and time on neutralizing antibodies against BoAHV-1. Killed vaccines produced low levels of antibodies against BoAHV-1, whereas modified live vaccines produced high levels of antibodies capable of providing neutralizing titers in the vaccinated animals, with the thermosensitive modified live vaccine showing the highest levels of antibodies.
Fil: Camargo, Luana. Universidade de Sao Paulo; Brasil
Fil: Vieira Franklin, Yasmin. Universidade de Sao Paulo; Brasil
Fil: Resende da Silva, Gustavo Feliciano. Universidade de Sao Paulo; Brasil
Fil: Ferreira Santos, Janaína. Universidade de Sao Paulo; Brasil
Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit.; Argentina
Fil: Wigdorovitz, Andrés. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit.; Argentina
Fil: Gomes, Viviani. Universidade de Sao Paulo; Brasil
description Bovine alphaherpesvirus 1 (BoAHV-1) infection affects the production and reproductive performance of dairy and beef livestock, resulting in considerable economic losses. In addition to biosecurity measures, vaccination programs are effective strategies for controlling and preventing BoAHV-1 infection and transmission. We evaluated the serological immune response against BoAHV- 1 induced by eight different formulations of commercial vaccines: three modified live vaccines and five killed vaccines containing BoAHV type 1 or types 1 and 5. In the first experiment, 50 BoAHV- 1-seronegative guinea pigs were assigned to eight groups; each individual in the treatment groups received two doses (one-fifth of the bovine dose). The second experiment was conducted using 29 crossbred Holstein × Gir heifers in four groups of six to nine animals each. The serological immune response against BoAHV-1 was measured using virus neutralization and enzyme-linked immunosorbent assays to measure the total IgG against BoAHV. We evaluated the effects of the vaccine, time, and interaction of the vaccine and time on neutralizing antibodies against BoAHV-1. Killed vaccines produced low levels of antibodies against BoAHV-1, whereas modified live vaccines produced high levels of antibodies capable of providing neutralizing titers in the vaccinated animals, with the thermosensitive modified live vaccine showing the highest levels of antibodies.
publishDate 2024
dc.date.none.fl_str_mv 2024-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/260926
Camargo, Luana; Vieira Franklin, Yasmin; Resende da Silva, Gustavo Feliciano; Ferreira Santos, Janaína; Parreño, Gladys Viviana; et al.; Serological Responses of Guinea Pigs and Heifers to Eight Different BoAHV-1 Vaccine Formulations; MDPI; Vaccines; 12; 6; 6-2024; 1-13
2076-393X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/260926
identifier_str_mv Camargo, Luana; Vieira Franklin, Yasmin; Resende da Silva, Gustavo Feliciano; Ferreira Santos, Janaína; Parreño, Gladys Viviana; et al.; Serological Responses of Guinea Pigs and Heifers to Eight Different BoAHV-1 Vaccine Formulations; MDPI; Vaccines; 12; 6; 6-2024; 1-13
2076-393X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-393X/12/6/615
info:eu-repo/semantics/altIdentifier/doi/10.3390/vaccines12060615
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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