Combination of Amphotericin B and Flucytosine against Neurotropic Species of Melanized Fungi Causing Primary Cerebral Phaeohyphomycosis

Autores
Deng, S.; Pan, W.; Liao, W.; de Hoog, G. S.; Gerrits van den Ende, A. H. G.; Vitale, Roxana Gabriela; Rafati, H.; Ilkit, M.; Van der Lee, A. H.; Rijs, A. J. M. M.; Verweij, P. E.; Seyedmousavi, S.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Primary central nervous system phaeohyphomycosis is a fatal fungal infection due mainly to the neurotropic melanized fungiCladophialophora bantiana,Rhinocladiella mackenziei, andExophiala dermatitidis.Despite the combination of surgery with antifungal treatment, the prognosis continues to be poor, with mortality rates ranging from 50 to 70%. Therefore, a search for a more-appropriate therapeutic approach is urgently needed. Ourin vitrostudies showed that with the combination of amphotericin B and flucytosine against these species, the median fractional inhibitory concentration (FIC) indices for strains ranged from 0.25 to 0.38, indicating synergy. By use of Bliss independence analysis, a significant degree of synergy was confirmed for all strains, with the sum ΔE ranging from 90.2 to 698.61%. No antagonism was observed. These results indicate that amphotericin B, in combination with flucytosine, may have a role in the treatment of primary cerebral infections caused by melanized fungi belonging to the orderChaetothyriales Furtherin vivostudies and clinical investigations to elucidate and confirm these observations are warranted.
Fil: Deng, S.. First Hospital of Xinjiang Medical University; China. Second Military Medical University. Shanghai Institute of Medical Mycology; China
Fil: Pan, W.. Second Military Medical University. Shanghai Institute of Medical Mycology; China
Fil: Liao, W.. Second Military Medical University. Shanghai Institute of Medical Mycology; China
Fil: de Hoog, G. S.. CBS-KNAW Fungal Biodiversity Centre; Países Bajos. University of Amsterdam; Países Bajos. King Abdulaziz University; Arabia Saudita
Fil: Gerrits van den Ende, A. H. G.. CBS-KNAW Fungal Biodiversity Centre; Países Bajos. University of Amsterdam; Países Bajos
Fil: Vitale, Roxana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Ramos Mejía"; Argentina
Fil: Rafati, H.. Erasmus University Medical Center; Países Bajos
Fil: Ilkit, M.. University of Çukurova; Turquía
Fil: Van der Lee, A. H.. Radboud University Medical Center; Países Bajos
Fil: Rijs, A. J. M. M.. Radboud University Medical Center; Países Bajos
Fil: Verweij, P. E.. Radboud University Medical Center; Países Bajos
Fil: Seyedmousavi, S.. Radboud University Medical Center; Países Bajos. Erasmus University Medical Center; Países Bajos. Mazandaran University of Medical Sciences; Irán
Materia
Combination
Neurotropic Species
Melanized Fungi
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/45159
Acceder
id CONICETDig_c84e2462c31c0e9769fc0c0e7a5e0541
oai_identifier_str oai:ri.conicet.gov.ar:11336/45159
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Combination of Amphotericin B and Flucytosine against Neurotropic Species of Melanized Fungi Causing Primary Cerebral PhaeohyphomycosisDeng, S.Pan, W.Liao, W.de Hoog, G. S.Gerrits van den Ende, A. H. G.Vitale, Roxana GabrielaRafati, H.Ilkit, M.Van der Lee, A. H.Rijs, A. J. M. M.Verweij, P. E.Seyedmousavi, S.CombinationNeurotropic SpeciesMelanized Fungihttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Primary central nervous system phaeohyphomycosis is a fatal fungal infection due mainly to the neurotropic melanized fungiCladophialophora bantiana,Rhinocladiella mackenziei, andExophiala dermatitidis.Despite the combination of surgery with antifungal treatment, the prognosis continues to be poor, with mortality rates ranging from 50 to 70%. Therefore, a search for a more-appropriate therapeutic approach is urgently needed. Ourin vitrostudies showed that with the combination of amphotericin B and flucytosine against these species, the median fractional inhibitory concentration (FIC) indices for strains ranged from 0.25 to 0.38, indicating synergy. By use of Bliss independence analysis, a significant degree of synergy was confirmed for all strains, with the sum ΔE ranging from 90.2 to 698.61%. No antagonism was observed. These results indicate that amphotericin B, in combination with flucytosine, may have a role in the treatment of primary cerebral infections caused by melanized fungi belonging to the orderChaetothyriales Furtherin vivostudies and clinical investigations to elucidate and confirm these observations are warranted.Fil: Deng, S.. First Hospital of Xinjiang Medical University; China. Second Military Medical University. Shanghai Institute of Medical Mycology; ChinaFil: Pan, W.. Second Military Medical University. Shanghai Institute of Medical Mycology; ChinaFil: Liao, W.. Second Military Medical University. Shanghai Institute of Medical Mycology; ChinaFil: de Hoog, G. S.. CBS-KNAW Fungal Biodiversity Centre; Países Bajos. University of Amsterdam; Países Bajos. King Abdulaziz University; Arabia SauditaFil: Gerrits van den Ende, A. H. G.. CBS-KNAW Fungal Biodiversity Centre; Países Bajos. University of Amsterdam; Países BajosFil: Vitale, Roxana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Ramos Mejía"; ArgentinaFil: Rafati, H.. Erasmus University Medical Center; Países BajosFil: Ilkit, M.. University of Çukurova; TurquíaFil: Van der Lee, A. H.. Radboud University Medical Center; Países BajosFil: Rijs, A. J. M. M.. Radboud University Medical Center; Países BajosFil: Verweij, P. E.. Radboud University Medical Center; Países BajosFil: Seyedmousavi, S.. Radboud University Medical Center; Países Bajos. Erasmus University Medical Center; Países Bajos. Mazandaran University of Medical Sciences; IránAmerican Society for Microbiology2016-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/45159Deng, S.; Pan, W.; Liao, W.; de Hoog, G. S.; Gerrits van den Ende, A. H. G.; et al.; Combination of Amphotericin B and Flucytosine against Neurotropic Species of Melanized Fungi Causing Primary Cerebral Phaeohyphomycosis; American Society for Microbiology; Antimicrobial Agents and Chemotherapy; 60; 4; 4-2016; 2346-23510066-4804CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://aac.asm.org/content/60/4/2346.fullinfo:eu-repo/semantics/altIdentifier/doi/10.1128/AAC.02526-15info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:01:18Zoai:ri.conicet.gov.ar:11336/45159instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:01:18.697CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Combination of Amphotericin B and Flucytosine against Neurotropic Species of Melanized Fungi Causing Primary Cerebral Phaeohyphomycosis
title Combination of Amphotericin B and Flucytosine against Neurotropic Species of Melanized Fungi Causing Primary Cerebral Phaeohyphomycosis
spellingShingle Combination of Amphotericin B and Flucytosine against Neurotropic Species of Melanized Fungi Causing Primary Cerebral Phaeohyphomycosis
Deng, S.
Combination
Neurotropic Species
Melanized Fungi
title_short Combination of Amphotericin B and Flucytosine against Neurotropic Species of Melanized Fungi Causing Primary Cerebral Phaeohyphomycosis
title_full Combination of Amphotericin B and Flucytosine against Neurotropic Species of Melanized Fungi Causing Primary Cerebral Phaeohyphomycosis
title_fullStr Combination of Amphotericin B and Flucytosine against Neurotropic Species of Melanized Fungi Causing Primary Cerebral Phaeohyphomycosis
title_full_unstemmed Combination of Amphotericin B and Flucytosine against Neurotropic Species of Melanized Fungi Causing Primary Cerebral Phaeohyphomycosis
title_sort Combination of Amphotericin B and Flucytosine against Neurotropic Species of Melanized Fungi Causing Primary Cerebral Phaeohyphomycosis
dc.creator.none.fl_str_mv Deng, S.
Pan, W.
Liao, W.
de Hoog, G. S.
Gerrits van den Ende, A. H. G.
Vitale, Roxana Gabriela
Rafati, H.
Ilkit, M.
Van der Lee, A. H.
Rijs, A. J. M. M.
Verweij, P. E.
Seyedmousavi, S.
author Deng, S.
author_facet Deng, S.
Pan, W.
Liao, W.
de Hoog, G. S.
Gerrits van den Ende, A. H. G.
Vitale, Roxana Gabriela
Rafati, H.
Ilkit, M.
Van der Lee, A. H.
Rijs, A. J. M. M.
Verweij, P. E.
Seyedmousavi, S.
author_role author
author2 Pan, W.
Liao, W.
de Hoog, G. S.
Gerrits van den Ende, A. H. G.
Vitale, Roxana Gabriela
Rafati, H.
Ilkit, M.
Van der Lee, A. H.
Rijs, A. J. M. M.
Verweij, P. E.
Seyedmousavi, S.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Combination
Neurotropic Species
Melanized Fungi
topic Combination
Neurotropic Species
Melanized Fungi
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Primary central nervous system phaeohyphomycosis is a fatal fungal infection due mainly to the neurotropic melanized fungiCladophialophora bantiana,Rhinocladiella mackenziei, andExophiala dermatitidis.Despite the combination of surgery with antifungal treatment, the prognosis continues to be poor, with mortality rates ranging from 50 to 70%. Therefore, a search for a more-appropriate therapeutic approach is urgently needed. Ourin vitrostudies showed that with the combination of amphotericin B and flucytosine against these species, the median fractional inhibitory concentration (FIC) indices for strains ranged from 0.25 to 0.38, indicating synergy. By use of Bliss independence analysis, a significant degree of synergy was confirmed for all strains, with the sum ΔE ranging from 90.2 to 698.61%. No antagonism was observed. These results indicate that amphotericin B, in combination with flucytosine, may have a role in the treatment of primary cerebral infections caused by melanized fungi belonging to the orderChaetothyriales Furtherin vivostudies and clinical investigations to elucidate and confirm these observations are warranted.
Fil: Deng, S.. First Hospital of Xinjiang Medical University; China. Second Military Medical University. Shanghai Institute of Medical Mycology; China
Fil: Pan, W.. Second Military Medical University. Shanghai Institute of Medical Mycology; China
Fil: Liao, W.. Second Military Medical University. Shanghai Institute of Medical Mycology; China
Fil: de Hoog, G. S.. CBS-KNAW Fungal Biodiversity Centre; Países Bajos. University of Amsterdam; Países Bajos. King Abdulaziz University; Arabia Saudita
Fil: Gerrits van den Ende, A. H. G.. CBS-KNAW Fungal Biodiversity Centre; Países Bajos. University of Amsterdam; Países Bajos
Fil: Vitale, Roxana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Ramos Mejía"; Argentina
Fil: Rafati, H.. Erasmus University Medical Center; Países Bajos
Fil: Ilkit, M.. University of Çukurova; Turquía
Fil: Van der Lee, A. H.. Radboud University Medical Center; Países Bajos
Fil: Rijs, A. J. M. M.. Radboud University Medical Center; Países Bajos
Fil: Verweij, P. E.. Radboud University Medical Center; Países Bajos
Fil: Seyedmousavi, S.. Radboud University Medical Center; Países Bajos. Erasmus University Medical Center; Países Bajos. Mazandaran University of Medical Sciences; Irán
description Primary central nervous system phaeohyphomycosis is a fatal fungal infection due mainly to the neurotropic melanized fungiCladophialophora bantiana,Rhinocladiella mackenziei, andExophiala dermatitidis.Despite the combination of surgery with antifungal treatment, the prognosis continues to be poor, with mortality rates ranging from 50 to 70%. Therefore, a search for a more-appropriate therapeutic approach is urgently needed. Ourin vitrostudies showed that with the combination of amphotericin B and flucytosine against these species, the median fractional inhibitory concentration (FIC) indices for strains ranged from 0.25 to 0.38, indicating synergy. By use of Bliss independence analysis, a significant degree of synergy was confirmed for all strains, with the sum ΔE ranging from 90.2 to 698.61%. No antagonism was observed. These results indicate that amphotericin B, in combination with flucytosine, may have a role in the treatment of primary cerebral infections caused by melanized fungi belonging to the orderChaetothyriales Furtherin vivostudies and clinical investigations to elucidate and confirm these observations are warranted.
publishDate 2016
dc.date.none.fl_str_mv 2016-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/45159
Deng, S.; Pan, W.; Liao, W.; de Hoog, G. S.; Gerrits van den Ende, A. H. G.; et al.; Combination of Amphotericin B and Flucytosine against Neurotropic Species of Melanized Fungi Causing Primary Cerebral Phaeohyphomycosis; American Society for Microbiology; Antimicrobial Agents and Chemotherapy; 60; 4; 4-2016; 2346-2351
0066-4804
CONICET Digital
CONICET
url http://hdl.handle.net/11336/45159
identifier_str_mv Deng, S.; Pan, W.; Liao, W.; de Hoog, G. S.; Gerrits van den Ende, A. H. G.; et al.; Combination of Amphotericin B and Flucytosine against Neurotropic Species of Melanized Fungi Causing Primary Cerebral Phaeohyphomycosis; American Society for Microbiology; Antimicrobial Agents and Chemotherapy; 60; 4; 4-2016; 2346-2351
0066-4804
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://aac.asm.org/content/60/4/2346.full
info:eu-repo/semantics/altIdentifier/doi/10.1128/AAC.02526-15
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/msword
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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