Expression of fatty acid elongases in cells of the seminiferous epithelium

Autores
Oresti, Gerardo Martín; Furland, Natalia Edith; Vasquez, C.; Osses, N.; Reyes, J. G.; Aveldaño, Marta Isabel
Año de publicación
2014
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Fatty acid elongases play a crucial role in the biosynthesis of long-chain polyunsaturated fatty acids (PUFA) and in their further elongation to very long chain (VLC) PUFA. Male rat germ cell membranes contain glycerophospholipids with C18-C24 PUFA and sphingolipids with C26 to C32 PUFA. In the present study, the expression of seven members of the Elovl (elongation of very long chain fatty acids) gene family that encode elongases was surveyed in pachytene spermatocytes, round spermatids and Sertoli cells. The mRNAs of Elovl1, Elovl2, Elovl4, Elovl5, and Elovl6 were detected in all of these cells , all of them lacked Elovl3 expression, and Elovl7 was expressed only in the latter. As the ELOVL4 protein was previously shown to be responsible for the elongation of >C24 PUFA in the retina, the expression of this protein was also evaluated. During postnatal development, ELOVL4 was not detectable in testis up to P21, i.e., its time of appearance concurred with that of the first spermatocytes. Thereafter, the protein was evidently present in spermatocytes and spermatids, and was also faintly detected in Sertoli cells. As Elovl2 and Elovl5 are essential to form PUFA, and Elovl4 is required to elongate them, the joint expression of these elongases in spermatocytes and spermatids implies that they are functionally related, probably acting in sequence to produce the VLCPUFA of sphingolipids.
Fil: Oresti, Gerardo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Vasquez, C.. Universidad de Valparaíso; Chile
Fil: Osses, N.. Universidad de Valparaíso; Chile
Fil: Reyes, J. G.. Universidad de Valparaíso; Chile
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
50th Annual Meeting Argentine Society for Biochemistry and Molecular Biology
Rosario
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
TESTES
SPHINGOLIPIDS
VLCPUFA
ELOVL4
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/234248
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/234248
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Expression of fatty acid elongases in cells of the seminiferous epitheliumOresti, Gerardo MartínFurland, Natalia EdithVasquez, C.Osses, N.Reyes, J. G.Aveldaño, Marta IsabelTESTESSPHINGOLIPIDSVLCPUFAELOVL4https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Fatty acid elongases play a crucial role in the biosynthesis of long-chain polyunsaturated fatty acids (PUFA) and in their further elongation to very long chain (VLC) PUFA. Male rat germ cell membranes contain glycerophospholipids with C18-C24 PUFA and sphingolipids with C26 to C32 PUFA. In the present study, the expression of seven members of the Elovl (elongation of very long chain fatty acids) gene family that encode elongases was surveyed in pachytene spermatocytes, round spermatids and Sertoli cells. The mRNAs of Elovl1, Elovl2, Elovl4, Elovl5, and Elovl6 were detected in all of these cells , all of them lacked Elovl3 expression, and Elovl7 was expressed only in the latter. As the ELOVL4 protein was previously shown to be responsible for the elongation of >C24 PUFA in the retina, the expression of this protein was also evaluated. During postnatal development, ELOVL4 was not detectable in testis up to P21, i.e., its time of appearance concurred with that of the first spermatocytes. Thereafter, the protein was evidently present in spermatocytes and spermatids, and was also faintly detected in Sertoli cells. As Elovl2 and Elovl5 are essential to form PUFA, and Elovl4 is required to elongate them, the joint expression of these elongases in spermatocytes and spermatids implies that they are functionally related, probably acting in sequence to produce the VLCPUFA of sphingolipids.Fil: Oresti, Gerardo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Vasquez, C.. Universidad de Valparaíso; ChileFil: Osses, N.. Universidad de Valparaíso; ChileFil: Reyes, J. G.. Universidad de Valparaíso; ChileFil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina50th Annual Meeting Argentine Society for Biochemistry and Molecular BiologyRosarioArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularTech Science Press2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/234248Expression of fatty acid elongases in cells of the seminiferous epithelium; 50th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; Rosario; Argentina; 2014; 124-1240327-95451667-5746CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v38nSuppl.S/34067/pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:02:11Zoai:ri.conicet.gov.ar:11336/234248instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:02:11.506CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expression of fatty acid elongases in cells of the seminiferous epithelium
title Expression of fatty acid elongases in cells of the seminiferous epithelium
spellingShingle Expression of fatty acid elongases in cells of the seminiferous epithelium
Oresti, Gerardo Martín
TESTES
SPHINGOLIPIDS
VLCPUFA
ELOVL4
title_short Expression of fatty acid elongases in cells of the seminiferous epithelium
title_full Expression of fatty acid elongases in cells of the seminiferous epithelium
title_fullStr Expression of fatty acid elongases in cells of the seminiferous epithelium
title_full_unstemmed Expression of fatty acid elongases in cells of the seminiferous epithelium
title_sort Expression of fatty acid elongases in cells of the seminiferous epithelium
dc.creator.none.fl_str_mv Oresti, Gerardo Martín
Furland, Natalia Edith
Vasquez, C.
Osses, N.
Reyes, J. G.
Aveldaño, Marta Isabel
author Oresti, Gerardo Martín
author_facet Oresti, Gerardo Martín
Furland, Natalia Edith
Vasquez, C.
Osses, N.
Reyes, J. G.
Aveldaño, Marta Isabel
author_role author
author2 Furland, Natalia Edith
Vasquez, C.
Osses, N.
Reyes, J. G.
Aveldaño, Marta Isabel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv TESTES
SPHINGOLIPIDS
VLCPUFA
ELOVL4
topic TESTES
SPHINGOLIPIDS
VLCPUFA
ELOVL4
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Fatty acid elongases play a crucial role in the biosynthesis of long-chain polyunsaturated fatty acids (PUFA) and in their further elongation to very long chain (VLC) PUFA. Male rat germ cell membranes contain glycerophospholipids with C18-C24 PUFA and sphingolipids with C26 to C32 PUFA. In the present study, the expression of seven members of the Elovl (elongation of very long chain fatty acids) gene family that encode elongases was surveyed in pachytene spermatocytes, round spermatids and Sertoli cells. The mRNAs of Elovl1, Elovl2, Elovl4, Elovl5, and Elovl6 were detected in all of these cells , all of them lacked Elovl3 expression, and Elovl7 was expressed only in the latter. As the ELOVL4 protein was previously shown to be responsible for the elongation of >C24 PUFA in the retina, the expression of this protein was also evaluated. During postnatal development, ELOVL4 was not detectable in testis up to P21, i.e., its time of appearance concurred with that of the first spermatocytes. Thereafter, the protein was evidently present in spermatocytes and spermatids, and was also faintly detected in Sertoli cells. As Elovl2 and Elovl5 are essential to form PUFA, and Elovl4 is required to elongate them, the joint expression of these elongases in spermatocytes and spermatids implies that they are functionally related, probably acting in sequence to produce the VLCPUFA of sphingolipids.
Fil: Oresti, Gerardo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Vasquez, C.. Universidad de Valparaíso; Chile
Fil: Osses, N.. Universidad de Valparaíso; Chile
Fil: Reyes, J. G.. Universidad de Valparaíso; Chile
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
50th Annual Meeting Argentine Society for Biochemistry and Molecular Biology
Rosario
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description Fatty acid elongases play a crucial role in the biosynthesis of long-chain polyunsaturated fatty acids (PUFA) and in their further elongation to very long chain (VLC) PUFA. Male rat germ cell membranes contain glycerophospholipids with C18-C24 PUFA and sphingolipids with C26 to C32 PUFA. In the present study, the expression of seven members of the Elovl (elongation of very long chain fatty acids) gene family that encode elongases was surveyed in pachytene spermatocytes, round spermatids and Sertoli cells. The mRNAs of Elovl1, Elovl2, Elovl4, Elovl5, and Elovl6 were detected in all of these cells , all of them lacked Elovl3 expression, and Elovl7 was expressed only in the latter. As the ELOVL4 protein was previously shown to be responsible for the elongation of >C24 PUFA in the retina, the expression of this protein was also evaluated. During postnatal development, ELOVL4 was not detectable in testis up to P21, i.e., its time of appearance concurred with that of the first spermatocytes. Thereafter, the protein was evidently present in spermatocytes and spermatids, and was also faintly detected in Sertoli cells. As Elovl2 and Elovl5 are essential to form PUFA, and Elovl4 is required to elongate them, the joint expression of these elongases in spermatocytes and spermatids implies that they are functionally related, probably acting in sequence to produce the VLCPUFA of sphingolipids.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/234248
Expression of fatty acid elongases in cells of the seminiferous epithelium; 50th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; Rosario; Argentina; 2014; 124-124
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/234248
identifier_str_mv Expression of fatty acid elongases in cells of the seminiferous epithelium; 50th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; Rosario; Argentina; 2014; 124-124
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v38nSuppl.S/34067/pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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