Berry Flesh and Skin Ripening Features in Vitis vinifera as Assessed by Transcriptional Profiling

Autores
Lijavetzky, Diego Claudio; Carbonell Bejerano, Pablo; Grimplet, Jerome; Bravo, Gema; Flores, Pilar; Fenoll, José; Hellín, Pilar; Oliveros, Juan Carlos; Martinez-Zapater, José M.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Ripening of fleshy fruits is a complex developmental process involving the differentiation of tissues with separate functions. During grapevine berry ripening, important processes contributing to table and wine grape quality take place, some of them flesh- or skin-specific. In this work, transcriptional profiles throughout flesh and skin ripening were followed during two different seasons in a table grape cultivar "Muscat Hamburg" to determine tissue-specific as well as common developmental programs. Methodology/Principal Findings: Using an updated GrapeGen Affymetrix GeneChip annotation based on grapevine 12Xv1 gene predictions, 2188 differentially accumulated transcripts between flesh and skin and 2839 transcripts differentially accumulated throughout ripening in the same manner in both tissues were identified. Transcriptional profiles were dominated by changes at the beginning of véraison which affect both pericarp tissues, although frequently delayed or with lower intensity in the skin than in the flesh. Functional enrichment analysis identified the decay on biosynthetic processes, photosynthesis, and transport as a major part of the program delayed in the skin. In addition, a higher number of functional categories, including several related to macromolecule transport and phenylpropanoid and lipid biosynthesis, were over-represented in transcripts accumulated to higher levels in the skin. Functional enrichment also indicated auxin, gibberellins, and bHLH transcription factors to take part in the regulation of pre-véraison processes in the pericarp whereas WRKY and C2H2 family transcription factors seems to more specifically participate in the regulation of skin and flesh ripening, respectively. Conclusions/Significance: A transcriptomic analysis indicates that a large part of the ripening program is shared by both pericarp tissues despite some components are delayed in the skin. In addition, important tissue differences are present from early stages prior to the ripening onset, including tissue-specific regulators. Altogether, these findings provide key elements to understand berry ripening and its differential regulation in flesh and skin.
Fil: Lijavetzky, Diego Claudio. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología. Departamento de Genética Molecular de Plantas; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Carbonell Bejerano, Pablo. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología. Departamento de Genética Molecular de Plantas; España. Universidad de La Rioja-Gobierno de La Rioja. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias de la Vid y del Vino ; España
Fil: Grimplet, Jerome. Universidad de La Rioja-Gobierno de La Rioja. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias de la Vid y del Vino ; España
Fil: Bravo, Gema. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología. Departamento de Genética Molecular de Plantas; España
Fil: Flores, Pilar. Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario. Estación Sericícola, Área de Biotecnología, ; España
Fil: Fenoll, José. Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario. Estación Sericícola, Área de Biotecnología, ; España
Fil: Hellín, Pilar. Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario. Estación Sericícola, Área de Biotecnología, ; España
Fil: Oliveros, Juan Carlos. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología. Departamento de Genética Molecular de Plantas; España
Fil: Martinez-Zapater, José M.. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología. Departamento de Genética Molecular de Plantas; España. Universidad de La Rioja-Gobierno de La Rioja. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias de la Vid y del Vino ; España
Materia
VITIS VINIFERA
MICROARRAYS
FLESH
SKIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41893
Acceder
id CONICETDig_c7fcaf3ed8722dc784994a233c5a52e5
oai_identifier_str oai:ri.conicet.gov.ar:11336/41893
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Berry Flesh and Skin Ripening Features in Vitis vinifera as Assessed by Transcriptional ProfilingLijavetzky, Diego ClaudioCarbonell Bejerano, PabloGrimplet, JeromeBravo, GemaFlores, PilarFenoll, JoséHellín, PilarOliveros, Juan CarlosMartinez-Zapater, José M.VITIS VINIFERAMICROARRAYSFLESHSKINhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Ripening of fleshy fruits is a complex developmental process involving the differentiation of tissues with separate functions. During grapevine berry ripening, important processes contributing to table and wine grape quality take place, some of them flesh- or skin-specific. In this work, transcriptional profiles throughout flesh and skin ripening were followed during two different seasons in a table grape cultivar "Muscat Hamburg" to determine tissue-specific as well as common developmental programs. Methodology/Principal Findings: Using an updated GrapeGen Affymetrix GeneChip annotation based on grapevine 12Xv1 gene predictions, 2188 differentially accumulated transcripts between flesh and skin and 2839 transcripts differentially accumulated throughout ripening in the same manner in both tissues were identified. Transcriptional profiles were dominated by changes at the beginning of véraison which affect both pericarp tissues, although frequently delayed or with lower intensity in the skin than in the flesh. Functional enrichment analysis identified the decay on biosynthetic processes, photosynthesis, and transport as a major part of the program delayed in the skin. In addition, a higher number of functional categories, including several related to macromolecule transport and phenylpropanoid and lipid biosynthesis, were over-represented in transcripts accumulated to higher levels in the skin. Functional enrichment also indicated auxin, gibberellins, and bHLH transcription factors to take part in the regulation of pre-véraison processes in the pericarp whereas WRKY and C2H2 family transcription factors seems to more specifically participate in the regulation of skin and flesh ripening, respectively. Conclusions/Significance: A transcriptomic analysis indicates that a large part of the ripening program is shared by both pericarp tissues despite some components are delayed in the skin. In addition, important tissue differences are present from early stages prior to the ripening onset, including tissue-specific regulators. Altogether, these findings provide key elements to understand berry ripening and its differential regulation in flesh and skin.Fil: Lijavetzky, Diego Claudio. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología. Departamento de Genética Molecular de Plantas; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Carbonell Bejerano, Pablo. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología. Departamento de Genética Molecular de Plantas; España. Universidad de La Rioja-Gobierno de La Rioja. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias de la Vid y del Vino ; EspañaFil: Grimplet, Jerome. Universidad de La Rioja-Gobierno de La Rioja. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias de la Vid y del Vino ; EspañaFil: Bravo, Gema. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología. Departamento de Genética Molecular de Plantas; EspañaFil: Flores, Pilar. Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario. Estación Sericícola, Área de Biotecnología, ; EspañaFil: Fenoll, José. Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario. Estación Sericícola, Área de Biotecnología, ; EspañaFil: Hellín, Pilar. Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario. Estación Sericícola, Área de Biotecnología, ; EspañaFil: Oliveros, Juan Carlos. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología. Departamento de Genética Molecular de Plantas; EspañaFil: Martinez-Zapater, José M.. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología. Departamento de Genética Molecular de Plantas; España. Universidad de La Rioja-Gobierno de La Rioja. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias de la Vid y del Vino ; EspañaPublic Library of Science2012-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41893Lijavetzky, Diego Claudio; Carbonell Bejerano, Pablo; Grimplet, Jerome; Bravo, Gema; Flores, Pilar; et al.; Berry Flesh and Skin Ripening Features in Vitis vinifera as Assessed by Transcriptional Profiling; Public Library of Science; Plos One; 7; 6; 5-2012; 1-151932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0039547info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0039547info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:09Zoai:ri.conicet.gov.ar:11336/41893instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:09.804CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Berry Flesh and Skin Ripening Features in Vitis vinifera as Assessed by Transcriptional Profiling
title Berry Flesh and Skin Ripening Features in Vitis vinifera as Assessed by Transcriptional Profiling
spellingShingle Berry Flesh and Skin Ripening Features in Vitis vinifera as Assessed by Transcriptional Profiling
Lijavetzky, Diego Claudio
VITIS VINIFERA
MICROARRAYS
FLESH
SKIN
title_short Berry Flesh and Skin Ripening Features in Vitis vinifera as Assessed by Transcriptional Profiling
title_full Berry Flesh and Skin Ripening Features in Vitis vinifera as Assessed by Transcriptional Profiling
title_fullStr Berry Flesh and Skin Ripening Features in Vitis vinifera as Assessed by Transcriptional Profiling
title_full_unstemmed Berry Flesh and Skin Ripening Features in Vitis vinifera as Assessed by Transcriptional Profiling
title_sort Berry Flesh and Skin Ripening Features in Vitis vinifera as Assessed by Transcriptional Profiling
dc.creator.none.fl_str_mv Lijavetzky, Diego Claudio
Carbonell Bejerano, Pablo
Grimplet, Jerome
Bravo, Gema
Flores, Pilar
Fenoll, José
Hellín, Pilar
Oliveros, Juan Carlos
Martinez-Zapater, José M.
author Lijavetzky, Diego Claudio
author_facet Lijavetzky, Diego Claudio
Carbonell Bejerano, Pablo
Grimplet, Jerome
Bravo, Gema
Flores, Pilar
Fenoll, José
Hellín, Pilar
Oliveros, Juan Carlos
Martinez-Zapater, José M.
author_role author
author2 Carbonell Bejerano, Pablo
Grimplet, Jerome
Bravo, Gema
Flores, Pilar
Fenoll, José
Hellín, Pilar
Oliveros, Juan Carlos
Martinez-Zapater, José M.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv VITIS VINIFERA
MICROARRAYS
FLESH
SKIN
topic VITIS VINIFERA
MICROARRAYS
FLESH
SKIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Ripening of fleshy fruits is a complex developmental process involving the differentiation of tissues with separate functions. During grapevine berry ripening, important processes contributing to table and wine grape quality take place, some of them flesh- or skin-specific. In this work, transcriptional profiles throughout flesh and skin ripening were followed during two different seasons in a table grape cultivar "Muscat Hamburg" to determine tissue-specific as well as common developmental programs. Methodology/Principal Findings: Using an updated GrapeGen Affymetrix GeneChip annotation based on grapevine 12Xv1 gene predictions, 2188 differentially accumulated transcripts between flesh and skin and 2839 transcripts differentially accumulated throughout ripening in the same manner in both tissues were identified. Transcriptional profiles were dominated by changes at the beginning of véraison which affect both pericarp tissues, although frequently delayed or with lower intensity in the skin than in the flesh. Functional enrichment analysis identified the decay on biosynthetic processes, photosynthesis, and transport as a major part of the program delayed in the skin. In addition, a higher number of functional categories, including several related to macromolecule transport and phenylpropanoid and lipid biosynthesis, were over-represented in transcripts accumulated to higher levels in the skin. Functional enrichment also indicated auxin, gibberellins, and bHLH transcription factors to take part in the regulation of pre-véraison processes in the pericarp whereas WRKY and C2H2 family transcription factors seems to more specifically participate in the regulation of skin and flesh ripening, respectively. Conclusions/Significance: A transcriptomic analysis indicates that a large part of the ripening program is shared by both pericarp tissues despite some components are delayed in the skin. In addition, important tissue differences are present from early stages prior to the ripening onset, including tissue-specific regulators. Altogether, these findings provide key elements to understand berry ripening and its differential regulation in flesh and skin.
Fil: Lijavetzky, Diego Claudio. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología. Departamento de Genética Molecular de Plantas; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Carbonell Bejerano, Pablo. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología. Departamento de Genética Molecular de Plantas; España. Universidad de La Rioja-Gobierno de La Rioja. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias de la Vid y del Vino ; España
Fil: Grimplet, Jerome. Universidad de La Rioja-Gobierno de La Rioja. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias de la Vid y del Vino ; España
Fil: Bravo, Gema. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología. Departamento de Genética Molecular de Plantas; España
Fil: Flores, Pilar. Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario. Estación Sericícola, Área de Biotecnología, ; España
Fil: Fenoll, José. Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario. Estación Sericícola, Área de Biotecnología, ; España
Fil: Hellín, Pilar. Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario. Estación Sericícola, Área de Biotecnología, ; España
Fil: Oliveros, Juan Carlos. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología. Departamento de Genética Molecular de Plantas; España
Fil: Martinez-Zapater, José M.. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología. Departamento de Genética Molecular de Plantas; España. Universidad de La Rioja-Gobierno de La Rioja. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias de la Vid y del Vino ; España
description Background: Ripening of fleshy fruits is a complex developmental process involving the differentiation of tissues with separate functions. During grapevine berry ripening, important processes contributing to table and wine grape quality take place, some of them flesh- or skin-specific. In this work, transcriptional profiles throughout flesh and skin ripening were followed during two different seasons in a table grape cultivar "Muscat Hamburg" to determine tissue-specific as well as common developmental programs. Methodology/Principal Findings: Using an updated GrapeGen Affymetrix GeneChip annotation based on grapevine 12Xv1 gene predictions, 2188 differentially accumulated transcripts between flesh and skin and 2839 transcripts differentially accumulated throughout ripening in the same manner in both tissues were identified. Transcriptional profiles were dominated by changes at the beginning of véraison which affect both pericarp tissues, although frequently delayed or with lower intensity in the skin than in the flesh. Functional enrichment analysis identified the decay on biosynthetic processes, photosynthesis, and transport as a major part of the program delayed in the skin. In addition, a higher number of functional categories, including several related to macromolecule transport and phenylpropanoid and lipid biosynthesis, were over-represented in transcripts accumulated to higher levels in the skin. Functional enrichment also indicated auxin, gibberellins, and bHLH transcription factors to take part in the regulation of pre-véraison processes in the pericarp whereas WRKY and C2H2 family transcription factors seems to more specifically participate in the regulation of skin and flesh ripening, respectively. Conclusions/Significance: A transcriptomic analysis indicates that a large part of the ripening program is shared by both pericarp tissues despite some components are delayed in the skin. In addition, important tissue differences are present from early stages prior to the ripening onset, including tissue-specific regulators. Altogether, these findings provide key elements to understand berry ripening and its differential regulation in flesh and skin.
publishDate 2012
dc.date.none.fl_str_mv 2012-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41893
Lijavetzky, Diego Claudio; Carbonell Bejerano, Pablo; Grimplet, Jerome; Bravo, Gema; Flores, Pilar; et al.; Berry Flesh and Skin Ripening Features in Vitis vinifera as Assessed by Transcriptional Profiling; Public Library of Science; Plos One; 7; 6; 5-2012; 1-15
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41893
identifier_str_mv Lijavetzky, Diego Claudio; Carbonell Bejerano, Pablo; Grimplet, Jerome; Bravo, Gema; Flores, Pilar; et al.; Berry Flesh and Skin Ripening Features in Vitis vinifera as Assessed by Transcriptional Profiling; Public Library of Science; Plos One; 7; 6; 5-2012; 1-15
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0039547
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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