Dissecting the transcriptional response to elicitors in Vitis vinifera cells

Autores
Almagro, Lorena; Carbonell Bejerano, Pablo; Belchí Navarro, Sarai; Bru, Róque; Martínez Zapater, José M.; Lijavetzky, Diego Claudio; Pedreño, María A.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The high effectiveness of cyclic oligosaccharides like cyclodextrins in the production of trans-resveratrol in Vitis vinifera cell cultures is enhanced in the presence of methyl jasmonate. In order to dissect the basis of the interactions among the elicitation responses triggered by these two compounds, a transcriptional analysis of grapevine cell cultures treated with cyclodextrins and methyl jasmonate separately or in combination was carried out. The results showed that the activation of genes encoding enzymes from phenylpropanoid and stilbene biosynthesis induced by cyclodextrins alone was partially enhanced in the presence of methyl jasmonate, which correlated with their effects on trans-resveratrol production. In addition, the protein translation and cell cycle regulation were more highly repressed in cells treated with cyclodextrins than in those treated with methyl jasmonate, and this response was enhanced in the combined treatment. Ethylene signalling was activated by all treatments, while jasmonate signalling and salicylic acid conjugation were activated only in the presence of methyl jasmonate and cyclodextrins, respectively. Moreover, the combined treatment resulted in a crosstalk between the signalling cascades activated by cyclodextrins and methyl jasmonate which in turn, provoked the activation of additional regulatory pathways involving the up-regulation of MYB15, NAC and WRKY transcription factors, protein kinases and calcium signal transducers. All these results suggest that both elicitors cause an activation of the secondary metabolism in detriment of basic cell processes like the primary metabolism or cell division. Crosstalk between cyclodextrins and methyl jasmonate-induced signalling provokes an intensification of these responses resulting in a greater trans-resveratrol production.
Fil: Almagro, Lorena. Universidad de Murcia; España
Fil: Carbonell Bejerano, Pablo. Universidad de La Rioja. Instituto de Ciencias de la Vid y del Vino; España
Fil: Belchí Navarro, Sarai. Universidad de Murcia; España
Fil: Bru, Róque. Universidad de Alicante; España
Fil: Martínez Zapater, José M.. Universidad de La Rioja. Instituto de Ciencias de la Vid y del Vino; España. Consejo Superior de Investigaciones Cientificas; España
Fil: Lijavetzky, Diego Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Pedreño, María A.. Universidad de Murcia; España
Materia
VITIS VINIFERA
CELL CULTURE
GENOMICS
GENETIC REGULATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/22413
Acceder
id CONICETDig_4e8fc2249337f7236ccfffea770c65ec
oai_identifier_str oai:ri.conicet.gov.ar:11336/22413
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Dissecting the transcriptional response to elicitors in Vitis vinifera cellsAlmagro, LorenaCarbonell Bejerano, PabloBelchí Navarro, SaraiBru, RóqueMartínez Zapater, José M.Lijavetzky, Diego ClaudioPedreño, María A.VITIS VINIFERACELL CULTUREGENOMICSGENETIC REGULATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The high effectiveness of cyclic oligosaccharides like cyclodextrins in the production of trans-resveratrol in Vitis vinifera cell cultures is enhanced in the presence of methyl jasmonate. In order to dissect the basis of the interactions among the elicitation responses triggered by these two compounds, a transcriptional analysis of grapevine cell cultures treated with cyclodextrins and methyl jasmonate separately or in combination was carried out. The results showed that the activation of genes encoding enzymes from phenylpropanoid and stilbene biosynthesis induced by cyclodextrins alone was partially enhanced in the presence of methyl jasmonate, which correlated with their effects on trans-resveratrol production. In addition, the protein translation and cell cycle regulation were more highly repressed in cells treated with cyclodextrins than in those treated with methyl jasmonate, and this response was enhanced in the combined treatment. Ethylene signalling was activated by all treatments, while jasmonate signalling and salicylic acid conjugation were activated only in the presence of methyl jasmonate and cyclodextrins, respectively. Moreover, the combined treatment resulted in a crosstalk between the signalling cascades activated by cyclodextrins and methyl jasmonate which in turn, provoked the activation of additional regulatory pathways involving the up-regulation of MYB15, NAC and WRKY transcription factors, protein kinases and calcium signal transducers. All these results suggest that both elicitors cause an activation of the secondary metabolism in detriment of basic cell processes like the primary metabolism or cell division. Crosstalk between cyclodextrins and methyl jasmonate-induced signalling provokes an intensification of these responses resulting in a greater trans-resveratrol production.Fil: Almagro, Lorena. Universidad de Murcia; EspañaFil: Carbonell Bejerano, Pablo. Universidad de La Rioja. Instituto de Ciencias de la Vid y del Vino; EspañaFil: Belchí Navarro, Sarai. Universidad de Murcia; EspañaFil: Bru, Róque. Universidad de Alicante; EspañaFil: Martínez Zapater, José M.. Universidad de La Rioja. Instituto de Ciencias de la Vid y del Vino; España. Consejo Superior de Investigaciones Cientificas; EspañaFil: Lijavetzky, Diego Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Pedreño, María A.. Universidad de Murcia; EspañaPublic Library of Science2014-10-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/22413Almagro, Lorena; Carbonell Bejerano, Pablo; Belchí Navarro, Sarai; Bru, Róque; Martínez Zapater, José M.; et al.; Dissecting the transcriptional response to elicitors in Vitis vinifera cells; Public Library of Science; Plos One; 9; 10; 14-10-2014; 1-111932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://dx.plos.org/10.1371/journal.pone.0109777info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0109777info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:42Zoai:ri.conicet.gov.ar:11336/22413instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:43.095CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dissecting the transcriptional response to elicitors in Vitis vinifera cells
title Dissecting the transcriptional response to elicitors in Vitis vinifera cells
spellingShingle Dissecting the transcriptional response to elicitors in Vitis vinifera cells
Almagro, Lorena
VITIS VINIFERA
CELL CULTURE
GENOMICS
GENETIC REGULATION
title_short Dissecting the transcriptional response to elicitors in Vitis vinifera cells
title_full Dissecting the transcriptional response to elicitors in Vitis vinifera cells
title_fullStr Dissecting the transcriptional response to elicitors in Vitis vinifera cells
title_full_unstemmed Dissecting the transcriptional response to elicitors in Vitis vinifera cells
title_sort Dissecting the transcriptional response to elicitors in Vitis vinifera cells
dc.creator.none.fl_str_mv Almagro, Lorena
Carbonell Bejerano, Pablo
Belchí Navarro, Sarai
Bru, Róque
Martínez Zapater, José M.
Lijavetzky, Diego Claudio
Pedreño, María A.
author Almagro, Lorena
author_facet Almagro, Lorena
Carbonell Bejerano, Pablo
Belchí Navarro, Sarai
Bru, Róque
Martínez Zapater, José M.
Lijavetzky, Diego Claudio
Pedreño, María A.
author_role author
author2 Carbonell Bejerano, Pablo
Belchí Navarro, Sarai
Bru, Róque
Martínez Zapater, José M.
Lijavetzky, Diego Claudio
Pedreño, María A.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv VITIS VINIFERA
CELL CULTURE
GENOMICS
GENETIC REGULATION
topic VITIS VINIFERA
CELL CULTURE
GENOMICS
GENETIC REGULATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The high effectiveness of cyclic oligosaccharides like cyclodextrins in the production of trans-resveratrol in Vitis vinifera cell cultures is enhanced in the presence of methyl jasmonate. In order to dissect the basis of the interactions among the elicitation responses triggered by these two compounds, a transcriptional analysis of grapevine cell cultures treated with cyclodextrins and methyl jasmonate separately or in combination was carried out. The results showed that the activation of genes encoding enzymes from phenylpropanoid and stilbene biosynthesis induced by cyclodextrins alone was partially enhanced in the presence of methyl jasmonate, which correlated with their effects on trans-resveratrol production. In addition, the protein translation and cell cycle regulation were more highly repressed in cells treated with cyclodextrins than in those treated with methyl jasmonate, and this response was enhanced in the combined treatment. Ethylene signalling was activated by all treatments, while jasmonate signalling and salicylic acid conjugation were activated only in the presence of methyl jasmonate and cyclodextrins, respectively. Moreover, the combined treatment resulted in a crosstalk between the signalling cascades activated by cyclodextrins and methyl jasmonate which in turn, provoked the activation of additional regulatory pathways involving the up-regulation of MYB15, NAC and WRKY transcription factors, protein kinases and calcium signal transducers. All these results suggest that both elicitors cause an activation of the secondary metabolism in detriment of basic cell processes like the primary metabolism or cell division. Crosstalk between cyclodextrins and methyl jasmonate-induced signalling provokes an intensification of these responses resulting in a greater trans-resveratrol production.
Fil: Almagro, Lorena. Universidad de Murcia; España
Fil: Carbonell Bejerano, Pablo. Universidad de La Rioja. Instituto de Ciencias de la Vid y del Vino; España
Fil: Belchí Navarro, Sarai. Universidad de Murcia; España
Fil: Bru, Róque. Universidad de Alicante; España
Fil: Martínez Zapater, José M.. Universidad de La Rioja. Instituto de Ciencias de la Vid y del Vino; España. Consejo Superior de Investigaciones Cientificas; España
Fil: Lijavetzky, Diego Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Pedreño, María A.. Universidad de Murcia; España
description The high effectiveness of cyclic oligosaccharides like cyclodextrins in the production of trans-resveratrol in Vitis vinifera cell cultures is enhanced in the presence of methyl jasmonate. In order to dissect the basis of the interactions among the elicitation responses triggered by these two compounds, a transcriptional analysis of grapevine cell cultures treated with cyclodextrins and methyl jasmonate separately or in combination was carried out. The results showed that the activation of genes encoding enzymes from phenylpropanoid and stilbene biosynthesis induced by cyclodextrins alone was partially enhanced in the presence of methyl jasmonate, which correlated with their effects on trans-resveratrol production. In addition, the protein translation and cell cycle regulation were more highly repressed in cells treated with cyclodextrins than in those treated with methyl jasmonate, and this response was enhanced in the combined treatment. Ethylene signalling was activated by all treatments, while jasmonate signalling and salicylic acid conjugation were activated only in the presence of methyl jasmonate and cyclodextrins, respectively. Moreover, the combined treatment resulted in a crosstalk between the signalling cascades activated by cyclodextrins and methyl jasmonate which in turn, provoked the activation of additional regulatory pathways involving the up-regulation of MYB15, NAC and WRKY transcription factors, protein kinases and calcium signal transducers. All these results suggest that both elicitors cause an activation of the secondary metabolism in detriment of basic cell processes like the primary metabolism or cell division. Crosstalk between cyclodextrins and methyl jasmonate-induced signalling provokes an intensification of these responses resulting in a greater trans-resveratrol production.
publishDate 2014
dc.date.none.fl_str_mv 2014-10-14
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/22413
Almagro, Lorena; Carbonell Bejerano, Pablo; Belchí Navarro, Sarai; Bru, Róque; Martínez Zapater, José M.; et al.; Dissecting the transcriptional response to elicitors in Vitis vinifera cells; Public Library of Science; Plos One; 9; 10; 14-10-2014; 1-11
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/22413
identifier_str_mv Almagro, Lorena; Carbonell Bejerano, Pablo; Belchí Navarro, Sarai; Bru, Róque; Martínez Zapater, José M.; et al.; Dissecting the transcriptional response to elicitors in Vitis vinifera cells; Public Library of Science; Plos One; 9; 10; 14-10-2014; 1-11
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://dx.plos.org/10.1371/journal.pone.0109777
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0109777
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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