Expression and function of endocannabinoid receptors in the human adrenal cortex

Autores
Ziegler, C. G.; Mohn, Claudia Ester; Lamounier Zepter, V.; Besuhli, Valeria; Bornstein S. R.; Krug, A. W.; Ehrhart Bornstein, M.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Endogenous cannabinoids are important signaling molecules in neuroendocrine control of homeostatic and reproductive functions including stress response and energy metabolism. The hypothalamic paraventricular and supraoptic nuclei have been shown to release endocannabinoids, which act as retrograde messengers to modulate the synaptic release of glutamate during stress response. This study endeavors to elucidate possible interaction of the endocannabinoid system with the regulation of adrenocortical function at the adrenal level. Human adrenocortical NCI-H295R cells and normal human adrenal glands were used to study the possible effects of anandamide and cannabinoid receptor 1 (CB1) antagonist SR141716A on aldosterone and cortisol secretion. Our data indicate the expression of CB1 in human adrenal cortex and adrenocortical NCI-H295R cells; CB2 was not expressed. Furthermore, anandamide inhibited basal release and stimulated release of adrenocortical steroids (corticosterone and aldosterone); this effect was reversed by CB1 antagonist (SR141716A). Therefore, the endocannabinoid system at the level of the adrenal, can directly influence adrenocortical steroidogenesis.
Fil: Ziegler, C. G.. Carl Gustav Carus University Hospital; Alemania
Fil: Mohn, Claudia Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Lamounier Zepter, V.. Carl Gustav Carus University Hospital; Alemania
Fil: Besuhli, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Bornstein S. R.. Carl Gustav Carus University Hospital; Alemania
Fil: Krug, A. W.. Carl Gustav Carus University Hospital; Alemania
Fil: Ehrhart Bornstein, M.. Carl Gustav Carus University Hospital; Alemania
Materia
Anandamide
Cb1 Antagonist
Cortisol
Aldosterone
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/17684
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/17684
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Expression and function of endocannabinoid receptors in the human adrenal cortexZiegler, C. G.Mohn, Claudia EsterLamounier Zepter, V.Besuhli, ValeriaBornstein S. R.Krug, A. W.Ehrhart Bornstein, M.AnandamideCb1 AntagonistCortisolAldosteronehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Endogenous cannabinoids are important signaling molecules in neuroendocrine control of homeostatic and reproductive functions including stress response and energy metabolism. The hypothalamic paraventricular and supraoptic nuclei have been shown to release endocannabinoids, which act as retrograde messengers to modulate the synaptic release of glutamate during stress response. This study endeavors to elucidate possible interaction of the endocannabinoid system with the regulation of adrenocortical function at the adrenal level. Human adrenocortical NCI-H295R cells and normal human adrenal glands were used to study the possible effects of anandamide and cannabinoid receptor 1 (CB1) antagonist SR141716A on aldosterone and cortisol secretion. Our data indicate the expression of CB1 in human adrenal cortex and adrenocortical NCI-H295R cells; CB2 was not expressed. Furthermore, anandamide inhibited basal release and stimulated release of adrenocortical steroids (corticosterone and aldosterone); this effect was reversed by CB1 antagonist (SR141716A). Therefore, the endocannabinoid system at the level of the adrenal, can directly influence adrenocortical steroidogenesis.Fil: Ziegler, C. G.. Carl Gustav Carus University Hospital; AlemaniaFil: Mohn, Claudia Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Lamounier Zepter, V.. Carl Gustav Carus University Hospital; AlemaniaFil: Besuhli, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Bornstein S. R.. Carl Gustav Carus University Hospital; AlemaniaFil: Krug, A. W.. Carl Gustav Carus University Hospital; AlemaniaFil: Ehrhart Bornstein, M.. Carl Gustav Carus University Hospital; AlemaniaGeorg Thieme Verlag Kg2010-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/17684Ziegler, C. G.; Mohn, Claudia Ester; Lamounier Zepter, V.; Besuhli, Valeria; Bornstein S. R.; et al.; Expression and function of endocannabinoid receptors in the human adrenal cortex; Georg Thieme Verlag Kg; Hormone and Metabolic Research; 42; 2; 2-2010; 88-920018-5043enginfo:eu-repo/semantics/altIdentifier/url/https://www.thieme-connect.com/DOI/DOI?10.1055/s-0029-1241860info:eu-repo/semantics/altIdentifier/doi/10.1055/s-0029-1241860info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:28:26Zoai:ri.conicet.gov.ar:11336/17684instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:28:27.099CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expression and function of endocannabinoid receptors in the human adrenal cortex
title Expression and function of endocannabinoid receptors in the human adrenal cortex
spellingShingle Expression and function of endocannabinoid receptors in the human adrenal cortex
Ziegler, C. G.
Anandamide
Cb1 Antagonist
Cortisol
Aldosterone
title_short Expression and function of endocannabinoid receptors in the human adrenal cortex
title_full Expression and function of endocannabinoid receptors in the human adrenal cortex
title_fullStr Expression and function of endocannabinoid receptors in the human adrenal cortex
title_full_unstemmed Expression and function of endocannabinoid receptors in the human adrenal cortex
title_sort Expression and function of endocannabinoid receptors in the human adrenal cortex
dc.creator.none.fl_str_mv Ziegler, C. G.
Mohn, Claudia Ester
Lamounier Zepter, V.
Besuhli, Valeria
Bornstein S. R.
Krug, A. W.
Ehrhart Bornstein, M.
author Ziegler, C. G.
author_facet Ziegler, C. G.
Mohn, Claudia Ester
Lamounier Zepter, V.
Besuhli, Valeria
Bornstein S. R.
Krug, A. W.
Ehrhart Bornstein, M.
author_role author
author2 Mohn, Claudia Ester
Lamounier Zepter, V.
Besuhli, Valeria
Bornstein S. R.
Krug, A. W.
Ehrhart Bornstein, M.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Anandamide
Cb1 Antagonist
Cortisol
Aldosterone
topic Anandamide
Cb1 Antagonist
Cortisol
Aldosterone
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Endogenous cannabinoids are important signaling molecules in neuroendocrine control of homeostatic and reproductive functions including stress response and energy metabolism. The hypothalamic paraventricular and supraoptic nuclei have been shown to release endocannabinoids, which act as retrograde messengers to modulate the synaptic release of glutamate during stress response. This study endeavors to elucidate possible interaction of the endocannabinoid system with the regulation of adrenocortical function at the adrenal level. Human adrenocortical NCI-H295R cells and normal human adrenal glands were used to study the possible effects of anandamide and cannabinoid receptor 1 (CB1) antagonist SR141716A on aldosterone and cortisol secretion. Our data indicate the expression of CB1 in human adrenal cortex and adrenocortical NCI-H295R cells; CB2 was not expressed. Furthermore, anandamide inhibited basal release and stimulated release of adrenocortical steroids (corticosterone and aldosterone); this effect was reversed by CB1 antagonist (SR141716A). Therefore, the endocannabinoid system at the level of the adrenal, can directly influence adrenocortical steroidogenesis.
Fil: Ziegler, C. G.. Carl Gustav Carus University Hospital; Alemania
Fil: Mohn, Claudia Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Lamounier Zepter, V.. Carl Gustav Carus University Hospital; Alemania
Fil: Besuhli, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Bornstein S. R.. Carl Gustav Carus University Hospital; Alemania
Fil: Krug, A. W.. Carl Gustav Carus University Hospital; Alemania
Fil: Ehrhart Bornstein, M.. Carl Gustav Carus University Hospital; Alemania
description Endogenous cannabinoids are important signaling molecules in neuroendocrine control of homeostatic and reproductive functions including stress response and energy metabolism. The hypothalamic paraventricular and supraoptic nuclei have been shown to release endocannabinoids, which act as retrograde messengers to modulate the synaptic release of glutamate during stress response. This study endeavors to elucidate possible interaction of the endocannabinoid system with the regulation of adrenocortical function at the adrenal level. Human adrenocortical NCI-H295R cells and normal human adrenal glands were used to study the possible effects of anandamide and cannabinoid receptor 1 (CB1) antagonist SR141716A on aldosterone and cortisol secretion. Our data indicate the expression of CB1 in human adrenal cortex and adrenocortical NCI-H295R cells; CB2 was not expressed. Furthermore, anandamide inhibited basal release and stimulated release of adrenocortical steroids (corticosterone and aldosterone); this effect was reversed by CB1 antagonist (SR141716A). Therefore, the endocannabinoid system at the level of the adrenal, can directly influence adrenocortical steroidogenesis.
publishDate 2010
dc.date.none.fl_str_mv 2010-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/17684
Ziegler, C. G.; Mohn, Claudia Ester; Lamounier Zepter, V.; Besuhli, Valeria; Bornstein S. R.; et al.; Expression and function of endocannabinoid receptors in the human adrenal cortex; Georg Thieme Verlag Kg; Hormone and Metabolic Research; 42; 2; 2-2010; 88-92
0018-5043
url http://hdl.handle.net/11336/17684
identifier_str_mv Ziegler, C. G.; Mohn, Claudia Ester; Lamounier Zepter, V.; Besuhli, Valeria; Bornstein S. R.; et al.; Expression and function of endocannabinoid receptors in the human adrenal cortex; Georg Thieme Verlag Kg; Hormone and Metabolic Research; 42; 2; 2-2010; 88-92
0018-5043
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.thieme-connect.com/DOI/DOI?10.1055/s-0029-1241860
info:eu-repo/semantics/altIdentifier/doi/10.1055/s-0029-1241860
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Georg Thieme Verlag Kg
publisher.none.fl_str_mv Georg Thieme Verlag Kg
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082747840331776
score 13.22299

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