Behavioral and neurochemical long-term consequences of intranasal chlorpyrifos formulation administration in mice

Autores
Gallegos, Cristina Eugenia; Gumilar, Fernanda Andrea; Bartos, Mariana; Tisera, Gianina Rafaela; Baier, Carlos Javier
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Environmental pollution negatively affects cognitive performance and accelerates its decline associated with age. Inhalation or intranasal (IN) administration of xenobiotics constitutes a feasible route for substance delivery to the brain. Recently, we described that CF-1 mice exposed to a commercial formulation of chlorpyrifos (fCPF) exhibited recognition memory and anxiety impairments, together with disturbances in both, the redox balance and cholinergic and glutamatergic enzymes activities. In the present work, we studied the long-term consequences of IN fCPF administration (10 mg/kg, every other day, for 15 to 30 days) in mice. After a 4 to 8 months fCPF-free washout period, fur appearance improved, and the number of body injuries decreased in fCPF treated mice. Scores in recognition memory and anxiety levels were similar between the control and fCPF-treated group. Notably, spatial learning and memory were improved in fCPF treated group. Neurochemical markers of oxidative stress and the activities of enzymes belonging to the cholinergic and glutamatergic pathways showed significant differences from the control group even after 8 months. Neurochemical disturbances in IN fCPF-treated mice were also modeled using the human neuroblastoma cell line SH-SY5Y treated with fCPF and CPF. The obtained results suggest that previous exposure to fCPF induces long-term modifications in the brain biochemistry that could be responsible for the phenotypic and behavioral observations.
Fil: Gallegos, Cristina Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Gumilar, Fernanda Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Bartos, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Tisera, Gianina Rafaela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Baier, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
The 2nd International Electronic Conference on Biomedicines
Basilea
Suiza
Multidisciplinary Digital Publishing Institute
Biomedicines
Materia
CLORPYRIFOS
INTRANASAL ADMINISTRATION
MEMORY
ANXIETY
OXIDATIVE STRESS
GLUTAMATERGIC AND CHOLINERGIC SYSTEM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/228486
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/228486
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Behavioral and neurochemical long-term consequences of intranasal chlorpyrifos formulation administration in miceGallegos, Cristina EugeniaGumilar, Fernanda AndreaBartos, MarianaTisera, Gianina RafaelaBaier, Carlos JavierCLORPYRIFOSINTRANASAL ADMINISTRATIONMEMORYANXIETYOXIDATIVE STRESSGLUTAMATERGIC AND CHOLINERGIC SYSTEMhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Environmental pollution negatively affects cognitive performance and accelerates its decline associated with age. Inhalation or intranasal (IN) administration of xenobiotics constitutes a feasible route for substance delivery to the brain. Recently, we described that CF-1 mice exposed to a commercial formulation of chlorpyrifos (fCPF) exhibited recognition memory and anxiety impairments, together with disturbances in both, the redox balance and cholinergic and glutamatergic enzymes activities. In the present work, we studied the long-term consequences of IN fCPF administration (10 mg/kg, every other day, for 15 to 30 days) in mice. After a 4 to 8 months fCPF-free washout period, fur appearance improved, and the number of body injuries decreased in fCPF treated mice. Scores in recognition memory and anxiety levels were similar between the control and fCPF-treated group. Notably, spatial learning and memory were improved in fCPF treated group. Neurochemical markers of oxidative stress and the activities of enzymes belonging to the cholinergic and glutamatergic pathways showed significant differences from the control group even after 8 months. Neurochemical disturbances in IN fCPF-treated mice were also modeled using the human neuroblastoma cell line SH-SY5Y treated with fCPF and CPF. The obtained results suggest that previous exposure to fCPF induces long-term modifications in the brain biochemistry that could be responsible for the phenotypic and behavioral observations.Fil: Gallegos, Cristina Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Gumilar, Fernanda Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Bartos, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Tisera, Gianina Rafaela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Baier, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaThe 2nd International Electronic Conference on BiomedicinesBasileaSuizaMultidisciplinary Digital Publishing InstituteBiomedicinesMultidisciplinary Digital Publishing Institute2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/228486Behavioral and neurochemical long-term consequences of intranasal chlorpyrifos formulation administration in mice; The 2nd International Electronic Conference on Biomedicines; Basilea; Suiza; 2023; 1-12673-9992CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://ecb2023.sciforum.net/#session2624Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:10:49Zoai:ri.conicet.gov.ar:11336/228486instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:10:49.772CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Behavioral and neurochemical long-term consequences of intranasal chlorpyrifos formulation administration in mice
title Behavioral and neurochemical long-term consequences of intranasal chlorpyrifos formulation administration in mice
spellingShingle Behavioral and neurochemical long-term consequences of intranasal chlorpyrifos formulation administration in mice
Gallegos, Cristina Eugenia
CLORPYRIFOS
INTRANASAL ADMINISTRATION
MEMORY
ANXIETY
OXIDATIVE STRESS
GLUTAMATERGIC AND CHOLINERGIC SYSTEM
title_short Behavioral and neurochemical long-term consequences of intranasal chlorpyrifos formulation administration in mice
title_full Behavioral and neurochemical long-term consequences of intranasal chlorpyrifos formulation administration in mice
title_fullStr Behavioral and neurochemical long-term consequences of intranasal chlorpyrifos formulation administration in mice
title_full_unstemmed Behavioral and neurochemical long-term consequences of intranasal chlorpyrifos formulation administration in mice
title_sort Behavioral and neurochemical long-term consequences of intranasal chlorpyrifos formulation administration in mice
dc.creator.none.fl_str_mv Gallegos, Cristina Eugenia
Gumilar, Fernanda Andrea
Bartos, Mariana
Tisera, Gianina Rafaela
Baier, Carlos Javier
author Gallegos, Cristina Eugenia
author_facet Gallegos, Cristina Eugenia
Gumilar, Fernanda Andrea
Bartos, Mariana
Tisera, Gianina Rafaela
Baier, Carlos Javier
author_role author
author2 Gumilar, Fernanda Andrea
Bartos, Mariana
Tisera, Gianina Rafaela
Baier, Carlos Javier
author2_role author
author
author
author
dc.subject.none.fl_str_mv CLORPYRIFOS
INTRANASAL ADMINISTRATION
MEMORY
ANXIETY
OXIDATIVE STRESS
GLUTAMATERGIC AND CHOLINERGIC SYSTEM
topic CLORPYRIFOS
INTRANASAL ADMINISTRATION
MEMORY
ANXIETY
OXIDATIVE STRESS
GLUTAMATERGIC AND CHOLINERGIC SYSTEM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Environmental pollution negatively affects cognitive performance and accelerates its decline associated with age. Inhalation or intranasal (IN) administration of xenobiotics constitutes a feasible route for substance delivery to the brain. Recently, we described that CF-1 mice exposed to a commercial formulation of chlorpyrifos (fCPF) exhibited recognition memory and anxiety impairments, together with disturbances in both, the redox balance and cholinergic and glutamatergic enzymes activities. In the present work, we studied the long-term consequences of IN fCPF administration (10 mg/kg, every other day, for 15 to 30 days) in mice. After a 4 to 8 months fCPF-free washout period, fur appearance improved, and the number of body injuries decreased in fCPF treated mice. Scores in recognition memory and anxiety levels were similar between the control and fCPF-treated group. Notably, spatial learning and memory were improved in fCPF treated group. Neurochemical markers of oxidative stress and the activities of enzymes belonging to the cholinergic and glutamatergic pathways showed significant differences from the control group even after 8 months. Neurochemical disturbances in IN fCPF-treated mice were also modeled using the human neuroblastoma cell line SH-SY5Y treated with fCPF and CPF. The obtained results suggest that previous exposure to fCPF induces long-term modifications in the brain biochemistry that could be responsible for the phenotypic and behavioral observations.
Fil: Gallegos, Cristina Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Gumilar, Fernanda Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Bartos, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Tisera, Gianina Rafaela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Baier, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
The 2nd International Electronic Conference on Biomedicines
Basilea
Suiza
Multidisciplinary Digital Publishing Institute
Biomedicines
description Environmental pollution negatively affects cognitive performance and accelerates its decline associated with age. Inhalation or intranasal (IN) administration of xenobiotics constitutes a feasible route for substance delivery to the brain. Recently, we described that CF-1 mice exposed to a commercial formulation of chlorpyrifos (fCPF) exhibited recognition memory and anxiety impairments, together with disturbances in both, the redox balance and cholinergic and glutamatergic enzymes activities. In the present work, we studied the long-term consequences of IN fCPF administration (10 mg/kg, every other day, for 15 to 30 days) in mice. After a 4 to 8 months fCPF-free washout period, fur appearance improved, and the number of body injuries decreased in fCPF treated mice. Scores in recognition memory and anxiety levels were similar between the control and fCPF-treated group. Notably, spatial learning and memory were improved in fCPF treated group. Neurochemical markers of oxidative stress and the activities of enzymes belonging to the cholinergic and glutamatergic pathways showed significant differences from the control group even after 8 months. Neurochemical disturbances in IN fCPF-treated mice were also modeled using the human neuroblastoma cell line SH-SY5Y treated with fCPF and CPF. The obtained results suggest that previous exposure to fCPF induces long-term modifications in the brain biochemistry that could be responsible for the phenotypic and behavioral observations.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/228486
Behavioral and neurochemical long-term consequences of intranasal chlorpyrifos formulation administration in mice; The 2nd International Electronic Conference on Biomedicines; Basilea; Suiza; 2023; 1-1
2673-9992
CONICET Digital
CONICET
url http://hdl.handle.net/11336/228486
identifier_str_mv Behavioral and neurochemical long-term consequences of intranasal chlorpyrifos formulation administration in mice; The 2nd International Electronic Conference on Biomedicines; Basilea; Suiza; 2023; 1-1
2673-9992
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ecb2023.sciforum.net/#session2624
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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