Behavioral impairments following repeated intranasal glyphosate-based herbicide administration in mice

Autores
Baier, Carlos Javier; Gallegos, Cristina Eugenia; Raisman Vozari, Rita; Minetti, Silvia Alejandra
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Inhalation or intranasal (IN) administration of neurotoxicants could constitute a route of toxin delivery to the brain. Pesticides have been proposed as the main environmental factor associated with the etiology of neurodegenerative disorders. In Argentina, the area used for glyphosate (Gly)-resistant crops are sprayed annually with ~ 200 million liters of Gly-based herbicides (Gly-BHs). Gly residues are often found in the environment, and considering the frequency and amount of its applications, it is probable that the inhalation of Gly-BHs occurs. The present study investigates the neurobehavioral effects of repeated IN administration of Gly-BH in male CF-1 mice (~ 2 mg/nostrils/day) three days a week, during four weeks (50 mg/kg/day). Locomotor activity and anxiety levels were studied by the Open Field (OF) test. Anxiety was also analyzed through the plus maze (PM) test. Novel Object Recognition (NOR) test was used for recognition memory analysis. Repeated IN Gly-BH administration in mice decreased the ambulatory activity. Moreover, Gly-BH treated mice showed a pronounced increase in thigmotaxis, compared to control group, indicating higher anxiety levels. The anxiogenic behavior in Gly-BH treated mice was then confirmed by PM test. The recognition memory was significantly impaired after 6 h in the Gly-BH treated group. No differences were observed between both groups when the NOR test was performed 24 h after. The present study reveals that repeated IN exposure to Gly-BH in mice affects the central nervous system probably altering neurotransmission pathways that participate or regulate locomotor activity, anxiety and memory.
Fil: Baier, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Gallegos, Cristina Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Raisman Vozari, Rita. Centre National de la Recherche Scientifique; Francia. Universite Pierre et Marie Curie; Francia
Fil: Minetti, Silvia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Materia
Glyphosate-Based Herbicides
Intranasal Administration
Locomotor Activity
Anxiety
Memory
Mouse
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41030
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/41030
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Behavioral impairments following repeated intranasal glyphosate-based herbicide administration in miceBaier, Carlos JavierGallegos, Cristina EugeniaRaisman Vozari, RitaMinetti, Silvia AlejandraGlyphosate-Based HerbicidesIntranasal AdministrationLocomotor ActivityAnxietyMemoryMousehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Inhalation or intranasal (IN) administration of neurotoxicants could constitute a route of toxin delivery to the brain. Pesticides have been proposed as the main environmental factor associated with the etiology of neurodegenerative disorders. In Argentina, the area used for glyphosate (Gly)-resistant crops are sprayed annually with ~ 200 million liters of Gly-based herbicides (Gly-BHs). Gly residues are often found in the environment, and considering the frequency and amount of its applications, it is probable that the inhalation of Gly-BHs occurs. The present study investigates the neurobehavioral effects of repeated IN administration of Gly-BH in male CF-1 mice (~ 2 mg/nostrils/day) three days a week, during four weeks (50 mg/kg/day). Locomotor activity and anxiety levels were studied by the Open Field (OF) test. Anxiety was also analyzed through the plus maze (PM) test. Novel Object Recognition (NOR) test was used for recognition memory analysis. Repeated IN Gly-BH administration in mice decreased the ambulatory activity. Moreover, Gly-BH treated mice showed a pronounced increase in thigmotaxis, compared to control group, indicating higher anxiety levels. The anxiogenic behavior in Gly-BH treated mice was then confirmed by PM test. The recognition memory was significantly impaired after 6 h in the Gly-BH treated group. No differences were observed between both groups when the NOR test was performed 24 h after. The present study reveals that repeated IN exposure to Gly-BH in mice affects the central nervous system probably altering neurotransmission pathways that participate or regulate locomotor activity, anxiety and memory.Fil: Baier, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Gallegos, Cristina Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Raisman Vozari, Rita. Centre National de la Recherche Scientifique; Francia. Universite Pierre et Marie Curie; FranciaFil: Minetti, Silvia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaPergamon-Elsevier Science Ltd2017-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41030Baier, Carlos Javier; Gallegos, Cristina Eugenia; Raisman Vozari, Rita; Minetti, Silvia Alejandra; Behavioral impairments following repeated intranasal glyphosate-based herbicide administration in mice; Pergamon-Elsevier Science Ltd; Neurotoxicology and Teratology; 64; 11-2017; 63-720892-0362CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0892036217301526info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ntt.2017.10.004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:55Zoai:ri.conicet.gov.ar:11336/41030instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:55.72CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Behavioral impairments following repeated intranasal glyphosate-based herbicide administration in mice
title Behavioral impairments following repeated intranasal glyphosate-based herbicide administration in mice
spellingShingle Behavioral impairments following repeated intranasal glyphosate-based herbicide administration in mice
Baier, Carlos Javier
Glyphosate-Based Herbicides
Intranasal Administration
Locomotor Activity
Anxiety
Memory
Mouse
title_short Behavioral impairments following repeated intranasal glyphosate-based herbicide administration in mice
title_full Behavioral impairments following repeated intranasal glyphosate-based herbicide administration in mice
title_fullStr Behavioral impairments following repeated intranasal glyphosate-based herbicide administration in mice
title_full_unstemmed Behavioral impairments following repeated intranasal glyphosate-based herbicide administration in mice
title_sort Behavioral impairments following repeated intranasal glyphosate-based herbicide administration in mice
dc.creator.none.fl_str_mv Baier, Carlos Javier
Gallegos, Cristina Eugenia
Raisman Vozari, Rita
Minetti, Silvia Alejandra
author Baier, Carlos Javier
author_facet Baier, Carlos Javier
Gallegos, Cristina Eugenia
Raisman Vozari, Rita
Minetti, Silvia Alejandra
author_role author
author2 Gallegos, Cristina Eugenia
Raisman Vozari, Rita
Minetti, Silvia Alejandra
author2_role author
author
author
dc.subject.none.fl_str_mv Glyphosate-Based Herbicides
Intranasal Administration
Locomotor Activity
Anxiety
Memory
Mouse
topic Glyphosate-Based Herbicides
Intranasal Administration
Locomotor Activity
Anxiety
Memory
Mouse
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Inhalation or intranasal (IN) administration of neurotoxicants could constitute a route of toxin delivery to the brain. Pesticides have been proposed as the main environmental factor associated with the etiology of neurodegenerative disorders. In Argentina, the area used for glyphosate (Gly)-resistant crops are sprayed annually with ~ 200 million liters of Gly-based herbicides (Gly-BHs). Gly residues are often found in the environment, and considering the frequency and amount of its applications, it is probable that the inhalation of Gly-BHs occurs. The present study investigates the neurobehavioral effects of repeated IN administration of Gly-BH in male CF-1 mice (~ 2 mg/nostrils/day) three days a week, during four weeks (50 mg/kg/day). Locomotor activity and anxiety levels were studied by the Open Field (OF) test. Anxiety was also analyzed through the plus maze (PM) test. Novel Object Recognition (NOR) test was used for recognition memory analysis. Repeated IN Gly-BH administration in mice decreased the ambulatory activity. Moreover, Gly-BH treated mice showed a pronounced increase in thigmotaxis, compared to control group, indicating higher anxiety levels. The anxiogenic behavior in Gly-BH treated mice was then confirmed by PM test. The recognition memory was significantly impaired after 6 h in the Gly-BH treated group. No differences were observed between both groups when the NOR test was performed 24 h after. The present study reveals that repeated IN exposure to Gly-BH in mice affects the central nervous system probably altering neurotransmission pathways that participate or regulate locomotor activity, anxiety and memory.
Fil: Baier, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Gallegos, Cristina Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Raisman Vozari, Rita. Centre National de la Recherche Scientifique; Francia. Universite Pierre et Marie Curie; Francia
Fil: Minetti, Silvia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
description Inhalation or intranasal (IN) administration of neurotoxicants could constitute a route of toxin delivery to the brain. Pesticides have been proposed as the main environmental factor associated with the etiology of neurodegenerative disorders. In Argentina, the area used for glyphosate (Gly)-resistant crops are sprayed annually with ~ 200 million liters of Gly-based herbicides (Gly-BHs). Gly residues are often found in the environment, and considering the frequency and amount of its applications, it is probable that the inhalation of Gly-BHs occurs. The present study investigates the neurobehavioral effects of repeated IN administration of Gly-BH in male CF-1 mice (~ 2 mg/nostrils/day) three days a week, during four weeks (50 mg/kg/day). Locomotor activity and anxiety levels were studied by the Open Field (OF) test. Anxiety was also analyzed through the plus maze (PM) test. Novel Object Recognition (NOR) test was used for recognition memory analysis. Repeated IN Gly-BH administration in mice decreased the ambulatory activity. Moreover, Gly-BH treated mice showed a pronounced increase in thigmotaxis, compared to control group, indicating higher anxiety levels. The anxiogenic behavior in Gly-BH treated mice was then confirmed by PM test. The recognition memory was significantly impaired after 6 h in the Gly-BH treated group. No differences were observed between both groups when the NOR test was performed 24 h after. The present study reveals that repeated IN exposure to Gly-BH in mice affects the central nervous system probably altering neurotransmission pathways that participate or regulate locomotor activity, anxiety and memory.
publishDate 2017
dc.date.none.fl_str_mv 2017-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41030
Baier, Carlos Javier; Gallegos, Cristina Eugenia; Raisman Vozari, Rita; Minetti, Silvia Alejandra; Behavioral impairments following repeated intranasal glyphosate-based herbicide administration in mice; Pergamon-Elsevier Science Ltd; Neurotoxicology and Teratology; 64; 11-2017; 63-72
0892-0362
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41030
identifier_str_mv Baier, Carlos Javier; Gallegos, Cristina Eugenia; Raisman Vozari, Rita; Minetti, Silvia Alejandra; Behavioral impairments following repeated intranasal glyphosate-based herbicide administration in mice; Pergamon-Elsevier Science Ltd; Neurotoxicology and Teratology; 64; 11-2017; 63-72
0892-0362
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0892036217301526
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ntt.2017.10.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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