Nitro-fatty acids reduce atherosclerosis in apolipoprotein E-deficient mice

Autores
Rudolph, Tanja K.; Rudolph, Volker; Edreira, Martin Miguel; Cole, Marsha P.; Bonacci, Gustavo Roberto; Schopfer, Francisco J.; Woodcock, Steven R.; Franek, Andreas; Pekarova, Michaela; Khoo, Nicholas K. H.; Hasty, Alyssa H.; Baldus, Stephan; Freeman, Bruce A.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective-: Inflammatory processes and foam cell formation are key determinants in the initiation and progression of atherosclerosis. Electrophilic nitro-fatty acids, byproducts of nitric oxide-and nitrite-dependent redox reactions of unsaturated fatty acids, exhibit antiinflammatory signaling actions in inflammatory and vascular cell model systems. The in vivo action of nitro-fatty acids in chronic inflammatory processes such as atherosclerosis remains to be elucidated.Methods and results-: Herein, we demonstrate that subcutaneously administered 9-and 10-nitro-octadecenoic acid (nitro-oleic acid) potently reduced atherosclerotic lesion formation in apolipoprotein E-deficient mice. Nitro-fatty acids did not modulate serum lipoprotein profiles. Immunostaining and gene expression analyses revealed that nitro-oleic acid attenuated lesion formation by suppressing tissue oxidant generation, inhibiting adhesion molecule expression, and decreasing vessel wall infiltration of inflammatory cells. In addition, nitro-oleic acid reduced foam cell formation by attenuating oxidized low-density lipoprotein-induced phosphorylation of signal transducer and activator of transcription-1, a transcription factor linked to foam cell formation in atherosclerotic plaques. Atherosclerotic lesions of nitro-oleic acid-treated animals also showed an increased content of collagen and α-smooth muscle actin, suggesting conferral of higher plaque stability. Conclusion-: These results reveal the antiatherogenic actions of electrophilic nitro-fatty acids in a murine model of atherosclerosis.
Fil: Rudolph, Tanja K.. University of Pittsburgh; Estados Unidos. University Heart Center Hamburg; Alemania
Fil: Rudolph, Volker. University of Pittsburgh; Estados Unidos. University Heart Center Hamburg; Alemania
Fil: Edreira, Martin Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of Pittsburgh; Estados Unidos
Fil: Cole, Marsha P.. University of Pittsburgh; Estados Unidos
Fil: Bonacci, Gustavo Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of Pittsburgh; Estados Unidos
Fil: Schopfer, Francisco J.. University of Pittsburgh; Estados Unidos
Fil: Woodcock, Steven R.. University of Pittsburgh; Estados Unidos
Fil: Franek, Andreas. University Heart Center Hamburg; Alemania
Fil: Pekarova, Michaela. Biology Centre of the Academy of Sciences of the Czech Republic; República Checa
Fil: Khoo, Nicholas K. H.. University of Pittsburgh; Estados Unidos
Fil: Hasty, Alyssa H.. Vanderbilt University Medical Center; Estados Unidos
Fil: Baldus, Stephan. University Heart Center Hamburg; Alemania
Fil: Freeman, Bruce A.. University of Pittsburgh; Estados Unidos
Materia
Atherosclerosis
Electrophile
Foam Cells
Inflammation
Nitro-Fatty Acids
Oxidative Stress
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79596
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/79596
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Nitro-fatty acids reduce atherosclerosis in apolipoprotein E-deficient miceRudolph, Tanja K.Rudolph, VolkerEdreira, Martin MiguelCole, Marsha P.Bonacci, Gustavo RobertoSchopfer, Francisco J.Woodcock, Steven R.Franek, AndreasPekarova, MichaelaKhoo, Nicholas K. H.Hasty, Alyssa H.Baldus, StephanFreeman, Bruce A.AtherosclerosisElectrophileFoam CellsInflammationNitro-Fatty AcidsOxidative Stresshttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Objective-: Inflammatory processes and foam cell formation are key determinants in the initiation and progression of atherosclerosis. Electrophilic nitro-fatty acids, byproducts of nitric oxide-and nitrite-dependent redox reactions of unsaturated fatty acids, exhibit antiinflammatory signaling actions in inflammatory and vascular cell model systems. The in vivo action of nitro-fatty acids in chronic inflammatory processes such as atherosclerosis remains to be elucidated.Methods and results-: Herein, we demonstrate that subcutaneously administered 9-and 10-nitro-octadecenoic acid (nitro-oleic acid) potently reduced atherosclerotic lesion formation in apolipoprotein E-deficient mice. Nitro-fatty acids did not modulate serum lipoprotein profiles. Immunostaining and gene expression analyses revealed that nitro-oleic acid attenuated lesion formation by suppressing tissue oxidant generation, inhibiting adhesion molecule expression, and decreasing vessel wall infiltration of inflammatory cells. In addition, nitro-oleic acid reduced foam cell formation by attenuating oxidized low-density lipoprotein-induced phosphorylation of signal transducer and activator of transcription-1, a transcription factor linked to foam cell formation in atherosclerotic plaques. Atherosclerotic lesions of nitro-oleic acid-treated animals also showed an increased content of collagen and α-smooth muscle actin, suggesting conferral of higher plaque stability. Conclusion-: These results reveal the antiatherogenic actions of electrophilic nitro-fatty acids in a murine model of atherosclerosis.Fil: Rudolph, Tanja K.. University of Pittsburgh; Estados Unidos. University Heart Center Hamburg; AlemaniaFil: Rudolph, Volker. University of Pittsburgh; Estados Unidos. University Heart Center Hamburg; AlemaniaFil: Edreira, Martin Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of Pittsburgh; Estados UnidosFil: Cole, Marsha P.. University of Pittsburgh; Estados UnidosFil: Bonacci, Gustavo Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of Pittsburgh; Estados UnidosFil: Schopfer, Francisco J.. University of Pittsburgh; Estados UnidosFil: Woodcock, Steven R.. University of Pittsburgh; Estados UnidosFil: Franek, Andreas. University Heart Center Hamburg; AlemaniaFil: Pekarova, Michaela. Biology Centre of the Academy of Sciences of the Czech Republic; República ChecaFil: Khoo, Nicholas K. H.. University of Pittsburgh; Estados UnidosFil: Hasty, Alyssa H.. Vanderbilt University Medical Center; Estados UnidosFil: Baldus, Stephan. University Heart Center Hamburg; AlemaniaFil: Freeman, Bruce A.. University of Pittsburgh; Estados UnidosLippincott Williams2010-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79596Rudolph, Tanja K.; Rudolph, Volker; Edreira, Martin Miguel; Cole, Marsha P.; Bonacci, Gustavo Roberto; et al.; Nitro-fatty acids reduce atherosclerosis in apolipoprotein E-deficient mice; Lippincott Williams; Arteriosclerosis, Thrombosis, and Vascular Biology; 30; 5; 5-2010; 938-9451079-5642CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ahajournals.org/doi/10.1161/ATVBAHA.109.201582info:eu-repo/semantics/altIdentifier/doi/10.1161/ATVBAHA.109.201582info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:10Zoai:ri.conicet.gov.ar:11336/79596instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:11.213CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nitro-fatty acids reduce atherosclerosis in apolipoprotein E-deficient mice
title Nitro-fatty acids reduce atherosclerosis in apolipoprotein E-deficient mice
spellingShingle Nitro-fatty acids reduce atherosclerosis in apolipoprotein E-deficient mice
Rudolph, Tanja K.
Atherosclerosis
Electrophile
Foam Cells
Inflammation
Nitro-Fatty Acids
Oxidative Stress
title_short Nitro-fatty acids reduce atherosclerosis in apolipoprotein E-deficient mice
title_full Nitro-fatty acids reduce atherosclerosis in apolipoprotein E-deficient mice
title_fullStr Nitro-fatty acids reduce atherosclerosis in apolipoprotein E-deficient mice
title_full_unstemmed Nitro-fatty acids reduce atherosclerosis in apolipoprotein E-deficient mice
title_sort Nitro-fatty acids reduce atherosclerosis in apolipoprotein E-deficient mice
dc.creator.none.fl_str_mv Rudolph, Tanja K.
Rudolph, Volker
Edreira, Martin Miguel
Cole, Marsha P.
Bonacci, Gustavo Roberto
Schopfer, Francisco J.
Woodcock, Steven R.
Franek, Andreas
Pekarova, Michaela
Khoo, Nicholas K. H.
Hasty, Alyssa H.
Baldus, Stephan
Freeman, Bruce A.
author Rudolph, Tanja K.
author_facet Rudolph, Tanja K.
Rudolph, Volker
Edreira, Martin Miguel
Cole, Marsha P.
Bonacci, Gustavo Roberto
Schopfer, Francisco J.
Woodcock, Steven R.
Franek, Andreas
Pekarova, Michaela
Khoo, Nicholas K. H.
Hasty, Alyssa H.
Baldus, Stephan
Freeman, Bruce A.
author_role author
author2 Rudolph, Volker
Edreira, Martin Miguel
Cole, Marsha P.
Bonacci, Gustavo Roberto
Schopfer, Francisco J.
Woodcock, Steven R.
Franek, Andreas
Pekarova, Michaela
Khoo, Nicholas K. H.
Hasty, Alyssa H.
Baldus, Stephan
Freeman, Bruce A.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Atherosclerosis
Electrophile
Foam Cells
Inflammation
Nitro-Fatty Acids
Oxidative Stress
topic Atherosclerosis
Electrophile
Foam Cells
Inflammation
Nitro-Fatty Acids
Oxidative Stress
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Objective-: Inflammatory processes and foam cell formation are key determinants in the initiation and progression of atherosclerosis. Electrophilic nitro-fatty acids, byproducts of nitric oxide-and nitrite-dependent redox reactions of unsaturated fatty acids, exhibit antiinflammatory signaling actions in inflammatory and vascular cell model systems. The in vivo action of nitro-fatty acids in chronic inflammatory processes such as atherosclerosis remains to be elucidated.Methods and results-: Herein, we demonstrate that subcutaneously administered 9-and 10-nitro-octadecenoic acid (nitro-oleic acid) potently reduced atherosclerotic lesion formation in apolipoprotein E-deficient mice. Nitro-fatty acids did not modulate serum lipoprotein profiles. Immunostaining and gene expression analyses revealed that nitro-oleic acid attenuated lesion formation by suppressing tissue oxidant generation, inhibiting adhesion molecule expression, and decreasing vessel wall infiltration of inflammatory cells. In addition, nitro-oleic acid reduced foam cell formation by attenuating oxidized low-density lipoprotein-induced phosphorylation of signal transducer and activator of transcription-1, a transcription factor linked to foam cell formation in atherosclerotic plaques. Atherosclerotic lesions of nitro-oleic acid-treated animals also showed an increased content of collagen and α-smooth muscle actin, suggesting conferral of higher plaque stability. Conclusion-: These results reveal the antiatherogenic actions of electrophilic nitro-fatty acids in a murine model of atherosclerosis.
Fil: Rudolph, Tanja K.. University of Pittsburgh; Estados Unidos. University Heart Center Hamburg; Alemania
Fil: Rudolph, Volker. University of Pittsburgh; Estados Unidos. University Heart Center Hamburg; Alemania
Fil: Edreira, Martin Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of Pittsburgh; Estados Unidos
Fil: Cole, Marsha P.. University of Pittsburgh; Estados Unidos
Fil: Bonacci, Gustavo Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of Pittsburgh; Estados Unidos
Fil: Schopfer, Francisco J.. University of Pittsburgh; Estados Unidos
Fil: Woodcock, Steven R.. University of Pittsburgh; Estados Unidos
Fil: Franek, Andreas. University Heart Center Hamburg; Alemania
Fil: Pekarova, Michaela. Biology Centre of the Academy of Sciences of the Czech Republic; República Checa
Fil: Khoo, Nicholas K. H.. University of Pittsburgh; Estados Unidos
Fil: Hasty, Alyssa H.. Vanderbilt University Medical Center; Estados Unidos
Fil: Baldus, Stephan. University Heart Center Hamburg; Alemania
Fil: Freeman, Bruce A.. University of Pittsburgh; Estados Unidos
description Objective-: Inflammatory processes and foam cell formation are key determinants in the initiation and progression of atherosclerosis. Electrophilic nitro-fatty acids, byproducts of nitric oxide-and nitrite-dependent redox reactions of unsaturated fatty acids, exhibit antiinflammatory signaling actions in inflammatory and vascular cell model systems. The in vivo action of nitro-fatty acids in chronic inflammatory processes such as atherosclerosis remains to be elucidated.Methods and results-: Herein, we demonstrate that subcutaneously administered 9-and 10-nitro-octadecenoic acid (nitro-oleic acid) potently reduced atherosclerotic lesion formation in apolipoprotein E-deficient mice. Nitro-fatty acids did not modulate serum lipoprotein profiles. Immunostaining and gene expression analyses revealed that nitro-oleic acid attenuated lesion formation by suppressing tissue oxidant generation, inhibiting adhesion molecule expression, and decreasing vessel wall infiltration of inflammatory cells. In addition, nitro-oleic acid reduced foam cell formation by attenuating oxidized low-density lipoprotein-induced phosphorylation of signal transducer and activator of transcription-1, a transcription factor linked to foam cell formation in atherosclerotic plaques. Atherosclerotic lesions of nitro-oleic acid-treated animals also showed an increased content of collagen and α-smooth muscle actin, suggesting conferral of higher plaque stability. Conclusion-: These results reveal the antiatherogenic actions of electrophilic nitro-fatty acids in a murine model of atherosclerosis.
publishDate 2010
dc.date.none.fl_str_mv 2010-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79596
Rudolph, Tanja K.; Rudolph, Volker; Edreira, Martin Miguel; Cole, Marsha P.; Bonacci, Gustavo Roberto; et al.; Nitro-fatty acids reduce atherosclerosis in apolipoprotein E-deficient mice; Lippincott Williams; Arteriosclerosis, Thrombosis, and Vascular Biology; 30; 5; 5-2010; 938-945
1079-5642
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79596
identifier_str_mv Rudolph, Tanja K.; Rudolph, Volker; Edreira, Martin Miguel; Cole, Marsha P.; Bonacci, Gustavo Roberto; et al.; Nitro-fatty acids reduce atherosclerosis in apolipoprotein E-deficient mice; Lippincott Williams; Arteriosclerosis, Thrombosis, and Vascular Biology; 30; 5; 5-2010; 938-945
1079-5642
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ahajournals.org/doi/10.1161/ATVBAHA.109.201582
info:eu-repo/semantics/altIdentifier/doi/10.1161/ATVBAHA.109.201582
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Lippincott Williams
publisher.none.fl_str_mv Lippincott Williams
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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