E-cadherin expression pattern during zebrafish embryonic epidermis development [version 3; referees: 3 approved]

Autores
Sampedro, Maria Florencia; Izaguirre, María Fernanda; Sigot, Valeria
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: E-cadherin is the major adhesion receptor in epithelial adherens junctions (AJs). On established epidermis, E-cadherin performs fine-tuned cell-cell contact remodeling to maintain tissue integrity, which is characterized by modulation of cell shape, size and packing density. In zebrafish, the organization and distribution of E-cadherin in AJs during embryonic epidermis development remain scarcely described. Methods: Combining classical immunofluorescence, deconvolution microscopy and 3D-segmentation of AJs in epithelial cells, a quantitative approach was implemented to assess the spatial and temporal distribution of E-cadherin across zebrafish epidermis between 24 and 72 hpf. Results: increasing levels of E-cadh protein parallel higher cell density and the appearance of hexagonal cells in the enveloping layer (EVL) as well as the establishments of new cell-cell contacts in the epidermal basal layer (EBL), being significantly between 31 and 48 hpf. Conclusions: Increasing levels of E-cadherin in AJs correlates with extensive changes in cell morphology towards hexagonal packing during the epidermis morphogenesis.
Fil: Sampedro, Maria Florencia. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; Argentina
Fil: Izaguirre, María Fernanda. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; Argentina
Fil: Sigot, Valeria. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; Argentina
Materia
3D SEGMENTATION
DECONVOLUTION
E-CADHERIN; ADHERENS JUNCTIONS; ZEBRAFISH EPIDERMIS; ENVELOPING LAYER (EVL)
EPIDERMIS BASAL LAYER (EBL)
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/116222
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/116222
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling E-cadherin expression pattern during zebrafish embryonic epidermis development [version 3; referees: 3 approved]Sampedro, Maria FlorenciaIzaguirre, María FernandaSigot, Valeria3D SEGMENTATIONDECONVOLUTIONE-CADHERIN; ADHERENS JUNCTIONS; ZEBRAFISH EPIDERMIS; ENVELOPING LAYER (EVL)EPIDERMIS BASAL LAYER (EBL)https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: E-cadherin is the major adhesion receptor in epithelial adherens junctions (AJs). On established epidermis, E-cadherin performs fine-tuned cell-cell contact remodeling to maintain tissue integrity, which is characterized by modulation of cell shape, size and packing density. In zebrafish, the organization and distribution of E-cadherin in AJs during embryonic epidermis development remain scarcely described. Methods: Combining classical immunofluorescence, deconvolution microscopy and 3D-segmentation of AJs in epithelial cells, a quantitative approach was implemented to assess the spatial and temporal distribution of E-cadherin across zebrafish epidermis between 24 and 72 hpf. Results: increasing levels of E-cadh protein parallel higher cell density and the appearance of hexagonal cells in the enveloping layer (EVL) as well as the establishments of new cell-cell contacts in the epidermal basal layer (EBL), being significantly between 31 and 48 hpf. Conclusions: Increasing levels of E-cadherin in AJs correlates with extensive changes in cell morphology towards hexagonal packing during the epidermis morphogenesis.Fil: Sampedro, Maria Florencia. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; ArgentinaFil: Izaguirre, María Fernanda. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; ArgentinaFil: Sigot, Valeria. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; ArgentinaF1000 Research Ltd2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/116222Sampedro, Maria Florencia; Izaguirre, María Fernanda; Sigot, Valeria; E-cadherin expression pattern during zebrafish embryonic epidermis development [version 3; referees: 3 approved]; F1000 Research Ltd; F1000Research; 7; 12-2018; 1489-14992046-1402CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://f1000research.com/articles/7-1489/v3info:eu-repo/semantics/altIdentifier/doi/10.12688/f1000research.15932.3info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:34Zoai:ri.conicet.gov.ar:11336/116222instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:35.058CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv E-cadherin expression pattern during zebrafish embryonic epidermis development [version 3; referees: 3 approved]
title E-cadherin expression pattern during zebrafish embryonic epidermis development [version 3; referees: 3 approved]
spellingShingle E-cadherin expression pattern during zebrafish embryonic epidermis development [version 3; referees: 3 approved]
Sampedro, Maria Florencia
3D SEGMENTATION
DECONVOLUTION
E-CADHERIN; ADHERENS JUNCTIONS; ZEBRAFISH EPIDERMIS; ENVELOPING LAYER (EVL)
EPIDERMIS BASAL LAYER (EBL)
title_short E-cadherin expression pattern during zebrafish embryonic epidermis development [version 3; referees: 3 approved]
title_full E-cadherin expression pattern during zebrafish embryonic epidermis development [version 3; referees: 3 approved]
title_fullStr E-cadherin expression pattern during zebrafish embryonic epidermis development [version 3; referees: 3 approved]
title_full_unstemmed E-cadherin expression pattern during zebrafish embryonic epidermis development [version 3; referees: 3 approved]
title_sort E-cadherin expression pattern during zebrafish embryonic epidermis development [version 3; referees: 3 approved]
dc.creator.none.fl_str_mv Sampedro, Maria Florencia
Izaguirre, María Fernanda
Sigot, Valeria
author Sampedro, Maria Florencia
author_facet Sampedro, Maria Florencia
Izaguirre, María Fernanda
Sigot, Valeria
author_role author
author2 Izaguirre, María Fernanda
Sigot, Valeria
author2_role author
author
dc.subject.none.fl_str_mv 3D SEGMENTATION
DECONVOLUTION
E-CADHERIN; ADHERENS JUNCTIONS; ZEBRAFISH EPIDERMIS; ENVELOPING LAYER (EVL)
EPIDERMIS BASAL LAYER (EBL)
topic 3D SEGMENTATION
DECONVOLUTION
E-CADHERIN; ADHERENS JUNCTIONS; ZEBRAFISH EPIDERMIS; ENVELOPING LAYER (EVL)
EPIDERMIS BASAL LAYER (EBL)
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: E-cadherin is the major adhesion receptor in epithelial adherens junctions (AJs). On established epidermis, E-cadherin performs fine-tuned cell-cell contact remodeling to maintain tissue integrity, which is characterized by modulation of cell shape, size and packing density. In zebrafish, the organization and distribution of E-cadherin in AJs during embryonic epidermis development remain scarcely described. Methods: Combining classical immunofluorescence, deconvolution microscopy and 3D-segmentation of AJs in epithelial cells, a quantitative approach was implemented to assess the spatial and temporal distribution of E-cadherin across zebrafish epidermis between 24 and 72 hpf. Results: increasing levels of E-cadh protein parallel higher cell density and the appearance of hexagonal cells in the enveloping layer (EVL) as well as the establishments of new cell-cell contacts in the epidermal basal layer (EBL), being significantly between 31 and 48 hpf. Conclusions: Increasing levels of E-cadherin in AJs correlates with extensive changes in cell morphology towards hexagonal packing during the epidermis morphogenesis.
Fil: Sampedro, Maria Florencia. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; Argentina
Fil: Izaguirre, María Fernanda. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; Argentina
Fil: Sigot, Valeria. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; Argentina
description Background: E-cadherin is the major adhesion receptor in epithelial adherens junctions (AJs). On established epidermis, E-cadherin performs fine-tuned cell-cell contact remodeling to maintain tissue integrity, which is characterized by modulation of cell shape, size and packing density. In zebrafish, the organization and distribution of E-cadherin in AJs during embryonic epidermis development remain scarcely described. Methods: Combining classical immunofluorescence, deconvolution microscopy and 3D-segmentation of AJs in epithelial cells, a quantitative approach was implemented to assess the spatial and temporal distribution of E-cadherin across zebrafish epidermis between 24 and 72 hpf. Results: increasing levels of E-cadh protein parallel higher cell density and the appearance of hexagonal cells in the enveloping layer (EVL) as well as the establishments of new cell-cell contacts in the epidermal basal layer (EBL), being significantly between 31 and 48 hpf. Conclusions: Increasing levels of E-cadherin in AJs correlates with extensive changes in cell morphology towards hexagonal packing during the epidermis morphogenesis.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/116222
Sampedro, Maria Florencia; Izaguirre, María Fernanda; Sigot, Valeria; E-cadherin expression pattern during zebrafish embryonic epidermis development [version 3; referees: 3 approved]; F1000 Research Ltd; F1000Research; 7; 12-2018; 1489-1499
2046-1402
CONICET Digital
CONICET
url http://hdl.handle.net/11336/116222
identifier_str_mv Sampedro, Maria Florencia; Izaguirre, María Fernanda; Sigot, Valeria; E-cadherin expression pattern during zebrafish embryonic epidermis development [version 3; referees: 3 approved]; F1000 Research Ltd; F1000Research; 7; 12-2018; 1489-1499
2046-1402
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://f1000research.com/articles/7-1489/v3
info:eu-repo/semantics/altIdentifier/doi/10.12688/f1000research.15932.3
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv F1000 Research Ltd
publisher.none.fl_str_mv F1000 Research Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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