Anionic Quantum Dots reveal actin-microridges in zebrafish epidermis

Autores
Sigot, Valeria; Cabral Filho, Paulo E.; Sampedro, Maria Florencia; Santos, Beate S.; Fontes, Adriana
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Enhancement of hydrophilicity and functionalization of CdTe QDs (Quantum Dots) via surface modifications have made them suitable to be used as specific probes for cell imaging. Applications for targeting cell surfaces have been widely demonstrated in vitro but their use in animal models is not trivial. Here, we reported the interaction of mercaptosuccinic-coated (MSA) CdTe QDs with the epidermis of living and Carnoy-fixed zebrafish embryos. QDs concentrate along adherent junctions and reveal the characteristic pattern of actin microridges at the apical surface of the enveloping layer. In our study, labeling with anionic QDs is attained within few minutes at submicromolar concentrations in whole mounted Carnoy-fixed zebrafish embryos, providing a faster approach compared with immunodetection or standard Phalloidin staining of actin for visualization by fluorescence microscopy.
Fil: Sigot, Valeria. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; Argentina. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina
Fil: Cabral Filho, Paulo E.. Universidade Federal de Pernambuco; Brasil
Fil: Sampedro, Maria Florencia. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; Argentina. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina
Fil: Santos, Beate S.. Universidade Federal de Pernambuco; Brasil
Fil: Fontes, Adriana. Universidade Federal de Pernambuco; Brasil
Materia
ACTIN MICRORIDGES
ANIONIC QUANTUM DOTS
ZEBRAFISH EPIDERMIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/142929
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Anionic Quantum Dots reveal actin-microridges in zebrafish epidermisSigot, ValeriaCabral Filho, Paulo E.Sampedro, Maria FlorenciaSantos, Beate S.Fontes, AdrianaACTIN MICRORIDGESANIONIC QUANTUM DOTSZEBRAFISH EPIDERMIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Enhancement of hydrophilicity and functionalization of CdTe QDs (Quantum Dots) via surface modifications have made them suitable to be used as specific probes for cell imaging. Applications for targeting cell surfaces have been widely demonstrated in vitro but their use in animal models is not trivial. Here, we reported the interaction of mercaptosuccinic-coated (MSA) CdTe QDs with the epidermis of living and Carnoy-fixed zebrafish embryos. QDs concentrate along adherent junctions and reveal the characteristic pattern of actin microridges at the apical surface of the enveloping layer. In our study, labeling with anionic QDs is attained within few minutes at submicromolar concentrations in whole mounted Carnoy-fixed zebrafish embryos, providing a faster approach compared with immunodetection or standard Phalloidin staining of actin for visualization by fluorescence microscopy.Fil: Sigot, Valeria. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; Argentina. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; ArgentinaFil: Cabral Filho, Paulo E.. Universidade Federal de Pernambuco; BrasilFil: Sampedro, Maria Florencia. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; Argentina. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; ArgentinaFil: Santos, Beate S.. Universidade Federal de Pernambuco; BrasilFil: Fontes, Adriana. Universidade Federal de Pernambuco; BrasilIOP Publishing2020-07-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/142929Sigot, Valeria; Cabral Filho, Paulo E.; Sampedro, Maria Florencia; Santos, Beate S.; Fontes, Adriana; Anionic Quantum Dots reveal actin-microridges in zebrafish epidermis; IOP Publishing; Methods and Applications in Fluorescence; 8; 3; 18-7-2020; 1-82050-6120CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://iopscience.iop.org/article/10.1088/2050-6120/ab9124info:eu-repo/semantics/altIdentifier/doi/10.1088/2050-6120/ab9124info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:28:21Zoai:ri.conicet.gov.ar:11336/142929instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:28:21.992CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Anionic Quantum Dots reveal actin-microridges in zebrafish epidermis
title Anionic Quantum Dots reveal actin-microridges in zebrafish epidermis
spellingShingle Anionic Quantum Dots reveal actin-microridges in zebrafish epidermis
Sigot, Valeria
ACTIN MICRORIDGES
ANIONIC QUANTUM DOTS
ZEBRAFISH EPIDERMIS
title_short Anionic Quantum Dots reveal actin-microridges in zebrafish epidermis
title_full Anionic Quantum Dots reveal actin-microridges in zebrafish epidermis
title_fullStr Anionic Quantum Dots reveal actin-microridges in zebrafish epidermis
title_full_unstemmed Anionic Quantum Dots reveal actin-microridges in zebrafish epidermis
title_sort Anionic Quantum Dots reveal actin-microridges in zebrafish epidermis
dc.creator.none.fl_str_mv Sigot, Valeria
Cabral Filho, Paulo E.
Sampedro, Maria Florencia
Santos, Beate S.
Fontes, Adriana
author Sigot, Valeria
author_facet Sigot, Valeria
Cabral Filho, Paulo E.
Sampedro, Maria Florencia
Santos, Beate S.
Fontes, Adriana
author_role author
author2 Cabral Filho, Paulo E.
Sampedro, Maria Florencia
Santos, Beate S.
Fontes, Adriana
author2_role author
author
author
author
dc.subject.none.fl_str_mv ACTIN MICRORIDGES
ANIONIC QUANTUM DOTS
ZEBRAFISH EPIDERMIS
topic ACTIN MICRORIDGES
ANIONIC QUANTUM DOTS
ZEBRAFISH EPIDERMIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Enhancement of hydrophilicity and functionalization of CdTe QDs (Quantum Dots) via surface modifications have made them suitable to be used as specific probes for cell imaging. Applications for targeting cell surfaces have been widely demonstrated in vitro but their use in animal models is not trivial. Here, we reported the interaction of mercaptosuccinic-coated (MSA) CdTe QDs with the epidermis of living and Carnoy-fixed zebrafish embryos. QDs concentrate along adherent junctions and reveal the characteristic pattern of actin microridges at the apical surface of the enveloping layer. In our study, labeling with anionic QDs is attained within few minutes at submicromolar concentrations in whole mounted Carnoy-fixed zebrafish embryos, providing a faster approach compared with immunodetection or standard Phalloidin staining of actin for visualization by fluorescence microscopy.
Fil: Sigot, Valeria. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; Argentina. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina
Fil: Cabral Filho, Paulo E.. Universidade Federal de Pernambuco; Brasil
Fil: Sampedro, Maria Florencia. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; Argentina. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina
Fil: Santos, Beate S.. Universidade Federal de Pernambuco; Brasil
Fil: Fontes, Adriana. Universidade Federal de Pernambuco; Brasil
description Enhancement of hydrophilicity and functionalization of CdTe QDs (Quantum Dots) via surface modifications have made them suitable to be used as specific probes for cell imaging. Applications for targeting cell surfaces have been widely demonstrated in vitro but their use in animal models is not trivial. Here, we reported the interaction of mercaptosuccinic-coated (MSA) CdTe QDs with the epidermis of living and Carnoy-fixed zebrafish embryos. QDs concentrate along adherent junctions and reveal the characteristic pattern of actin microridges at the apical surface of the enveloping layer. In our study, labeling with anionic QDs is attained within few minutes at submicromolar concentrations in whole mounted Carnoy-fixed zebrafish embryos, providing a faster approach compared with immunodetection or standard Phalloidin staining of actin for visualization by fluorescence microscopy.
publishDate 2020
dc.date.none.fl_str_mv 2020-07-18
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/142929
Sigot, Valeria; Cabral Filho, Paulo E.; Sampedro, Maria Florencia; Santos, Beate S.; Fontes, Adriana; Anionic Quantum Dots reveal actin-microridges in zebrafish epidermis; IOP Publishing; Methods and Applications in Fluorescence; 8; 3; 18-7-2020; 1-8
2050-6120
CONICET Digital
CONICET
url http://hdl.handle.net/11336/142929
identifier_str_mv Sigot, Valeria; Cabral Filho, Paulo E.; Sampedro, Maria Florencia; Santos, Beate S.; Fontes, Adriana; Anionic Quantum Dots reveal actin-microridges in zebrafish epidermis; IOP Publishing; Methods and Applications in Fluorescence; 8; 3; 18-7-2020; 1-8
2050-6120
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://iopscience.iop.org/article/10.1088/2050-6120/ab9124
info:eu-repo/semantics/altIdentifier/doi/10.1088/2050-6120/ab9124
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv IOP Publishing
publisher.none.fl_str_mv IOP Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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