Metal Enhanced fluorescence emission and Quenching protection effect with a host-guest Nanophotonic-supramolecular structure

Autores
Veglia, Alicia Viviana; Bracamonte, Angel Guillermo
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The functionalization of the nanoparticle's (NP) surface is one method for tuning their overall properties to fit targeted applications. We developed a nanosensor based on the specific supramolecular interactions between β-cyclodextrin (βCD) nanocavities and organic molecules of biological interests using the metal-enhanced fluorescence effect (MEF) as the detection signal. We grafted βCD, a typical macrocyclic host molecule that interacts specifically with different organic molecules and changes their physical properties (such as their fluorescence emission intensity), on gold NPs. To evaluate this nanosensor and the effect of the metallic core, we worked with a typical organic molecule, Rhodamine B (RhB), that has a strong association constant with βCD (5700 M-1) and is well-known to be quenched in the presence of cyclodextrins (CDs). The results show that, by grafting βCD on gold NPs, it is possible to increase the sensitivity of RhB detection by 70%, 80%, and 294% when compared with solutions in (1) a phosphate buffer, (2) with βCD, and (3) with Au NPs, respectively. These results show that the use of a supramolecular system attached to a metallic NP can interact specifically with a dye to enhance its fluorescence emission through the MEF effect. Moreover, this type of nanosystem can overcome the quenching of the signal by the matrix, such in the case of RhB with CDs. Eventually, this concept could be extended to other dyes with different quenching effects. For this reason, this type of nanosensor system could be used in the future to protect and enhance the dye emission of fluorophores in different biological media.
Fil: Veglia, Alicia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Bracamonte, Angel Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina. Laval University; Canadá
Materia
ENHANCED SENSITIVITY
GOLD NANOPARTICLES
METAL-ENHANCED FLUORESCENCE
NANOPHOTONIC-SUPRAMOLECULAR SYSTEMS
QUENCHING PROTECTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/90899
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/90899
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Metal Enhanced fluorescence emission and Quenching protection effect with a host-guest Nanophotonic-supramolecular structureVeglia, Alicia VivianaBracamonte, Angel GuillermoENHANCED SENSITIVITYGOLD NANOPARTICLESMETAL-ENHANCED FLUORESCENCENANOPHOTONIC-SUPRAMOLECULAR SYSTEMSQUENCHING PROTECTIONhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The functionalization of the nanoparticle's (NP) surface is one method for tuning their overall properties to fit targeted applications. We developed a nanosensor based on the specific supramolecular interactions between β-cyclodextrin (βCD) nanocavities and organic molecules of biological interests using the metal-enhanced fluorescence effect (MEF) as the detection signal. We grafted βCD, a typical macrocyclic host molecule that interacts specifically with different organic molecules and changes their physical properties (such as their fluorescence emission intensity), on gold NPs. To evaluate this nanosensor and the effect of the metallic core, we worked with a typical organic molecule, Rhodamine B (RhB), that has a strong association constant with βCD (5700 M-1) and is well-known to be quenched in the presence of cyclodextrins (CDs). The results show that, by grafting βCD on gold NPs, it is possible to increase the sensitivity of RhB detection by 70%, 80%, and 294% when compared with solutions in (1) a phosphate buffer, (2) with βCD, and (3) with Au NPs, respectively. These results show that the use of a supramolecular system attached to a metallic NP can interact specifically with a dye to enhance its fluorescence emission through the MEF effect. Moreover, this type of nanosystem can overcome the quenching of the signal by the matrix, such in the case of RhB with CDs. Eventually, this concept could be extended to other dyes with different quenching effects. For this reason, this type of nanosensor system could be used in the future to protect and enhance the dye emission of fluorophores in different biological media.Fil: Veglia, Alicia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Bracamonte, Angel Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina. Laval University; CanadáSociety of Photo-Optical Instrumentation Engineers2018-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/90899Veglia, Alicia Viviana; Bracamonte, Angel Guillermo; Metal Enhanced fluorescence emission and Quenching protection effect with a host-guest Nanophotonic-supramolecular structure; Society of Photo-Optical Instrumentation Engineers; Journal Of Nanophotonics; 12; 3; 7-20181934-2608CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.spiedigitallibrary.org/journals/journal-of-nanophotonics/volume-12/issue-03/033004/Metal-enhanced-fluorescence-emission-and-quenching-protection-effect-with-a/10.1117/1.JNP.12.033004.fullinfo:eu-repo/semantics/altIdentifier/doi/10.1117/1.JNP.12.033004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T12:33:03Zoai:ri.conicet.gov.ar:11336/90899instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 12:33:04.141CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Metal Enhanced fluorescence emission and Quenching protection effect with a host-guest Nanophotonic-supramolecular structure
title Metal Enhanced fluorescence emission and Quenching protection effect with a host-guest Nanophotonic-supramolecular structure
spellingShingle Metal Enhanced fluorescence emission and Quenching protection effect with a host-guest Nanophotonic-supramolecular structure
Veglia, Alicia Viviana
ENHANCED SENSITIVITY
GOLD NANOPARTICLES
METAL-ENHANCED FLUORESCENCE
NANOPHOTONIC-SUPRAMOLECULAR SYSTEMS
QUENCHING PROTECTION
title_short Metal Enhanced fluorescence emission and Quenching protection effect with a host-guest Nanophotonic-supramolecular structure
title_full Metal Enhanced fluorescence emission and Quenching protection effect with a host-guest Nanophotonic-supramolecular structure
title_fullStr Metal Enhanced fluorescence emission and Quenching protection effect with a host-guest Nanophotonic-supramolecular structure
title_full_unstemmed Metal Enhanced fluorescence emission and Quenching protection effect with a host-guest Nanophotonic-supramolecular structure
title_sort Metal Enhanced fluorescence emission and Quenching protection effect with a host-guest Nanophotonic-supramolecular structure
dc.creator.none.fl_str_mv Veglia, Alicia Viviana
Bracamonte, Angel Guillermo
author Veglia, Alicia Viviana
author_facet Veglia, Alicia Viviana
Bracamonte, Angel Guillermo
author_role author
author2 Bracamonte, Angel Guillermo
author2_role author
dc.subject.none.fl_str_mv ENHANCED SENSITIVITY
GOLD NANOPARTICLES
METAL-ENHANCED FLUORESCENCE
NANOPHOTONIC-SUPRAMOLECULAR SYSTEMS
QUENCHING PROTECTION
topic ENHANCED SENSITIVITY
GOLD NANOPARTICLES
METAL-ENHANCED FLUORESCENCE
NANOPHOTONIC-SUPRAMOLECULAR SYSTEMS
QUENCHING PROTECTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The functionalization of the nanoparticle's (NP) surface is one method for tuning their overall properties to fit targeted applications. We developed a nanosensor based on the specific supramolecular interactions between β-cyclodextrin (βCD) nanocavities and organic molecules of biological interests using the metal-enhanced fluorescence effect (MEF) as the detection signal. We grafted βCD, a typical macrocyclic host molecule that interacts specifically with different organic molecules and changes their physical properties (such as their fluorescence emission intensity), on gold NPs. To evaluate this nanosensor and the effect of the metallic core, we worked with a typical organic molecule, Rhodamine B (RhB), that has a strong association constant with βCD (5700 M-1) and is well-known to be quenched in the presence of cyclodextrins (CDs). The results show that, by grafting βCD on gold NPs, it is possible to increase the sensitivity of RhB detection by 70%, 80%, and 294% when compared with solutions in (1) a phosphate buffer, (2) with βCD, and (3) with Au NPs, respectively. These results show that the use of a supramolecular system attached to a metallic NP can interact specifically with a dye to enhance its fluorescence emission through the MEF effect. Moreover, this type of nanosystem can overcome the quenching of the signal by the matrix, such in the case of RhB with CDs. Eventually, this concept could be extended to other dyes with different quenching effects. For this reason, this type of nanosensor system could be used in the future to protect and enhance the dye emission of fluorophores in different biological media.
Fil: Veglia, Alicia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Bracamonte, Angel Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina. Laval University; Canadá
description The functionalization of the nanoparticle's (NP) surface is one method for tuning their overall properties to fit targeted applications. We developed a nanosensor based on the specific supramolecular interactions between β-cyclodextrin (βCD) nanocavities and organic molecules of biological interests using the metal-enhanced fluorescence effect (MEF) as the detection signal. We grafted βCD, a typical macrocyclic host molecule that interacts specifically with different organic molecules and changes their physical properties (such as their fluorescence emission intensity), on gold NPs. To evaluate this nanosensor and the effect of the metallic core, we worked with a typical organic molecule, Rhodamine B (RhB), that has a strong association constant with βCD (5700 M-1) and is well-known to be quenched in the presence of cyclodextrins (CDs). The results show that, by grafting βCD on gold NPs, it is possible to increase the sensitivity of RhB detection by 70%, 80%, and 294% when compared with solutions in (1) a phosphate buffer, (2) with βCD, and (3) with Au NPs, respectively. These results show that the use of a supramolecular system attached to a metallic NP can interact specifically with a dye to enhance its fluorescence emission through the MEF effect. Moreover, this type of nanosystem can overcome the quenching of the signal by the matrix, such in the case of RhB with CDs. Eventually, this concept could be extended to other dyes with different quenching effects. For this reason, this type of nanosensor system could be used in the future to protect and enhance the dye emission of fluorophores in different biological media.
publishDate 2018
dc.date.none.fl_str_mv 2018-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/90899
Veglia, Alicia Viviana; Bracamonte, Angel Guillermo; Metal Enhanced fluorescence emission and Quenching protection effect with a host-guest Nanophotonic-supramolecular structure; Society of Photo-Optical Instrumentation Engineers; Journal Of Nanophotonics; 12; 3; 7-2018
1934-2608
CONICET Digital
CONICET
url http://hdl.handle.net/11336/90899
identifier_str_mv Veglia, Alicia Viviana; Bracamonte, Angel Guillermo; Metal Enhanced fluorescence emission and Quenching protection effect with a host-guest Nanophotonic-supramolecular structure; Society of Photo-Optical Instrumentation Engineers; Journal Of Nanophotonics; 12; 3; 7-2018
1934-2608
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.spiedigitallibrary.org/journals/journal-of-nanophotonics/volume-12/issue-03/033004/Metal-enhanced-fluorescence-emission-and-quenching-protection-effect-with-a/10.1117/1.JNP.12.033004.full
info:eu-repo/semantics/altIdentifier/doi/10.1117/1.JNP.12.033004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society of Photo-Optical Instrumentation Engineers
publisher.none.fl_str_mv Society of Photo-Optical Instrumentation Engineers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.10058

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