Ultraluminescent gold core-shell nanoparticles applied to individual bacterial detection based on metal-enhanced fluorescence nanoimaging

Autores
Gontero, Daniela; Veglia, Alicia Viviana; Boudreau, Denis; Bracamonte, Angel Guillermo
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Gold core-shell nanoparticles were synthesized based on metallic cores, variable silica shell spacers covered with modified fluorescent silica layers. Ultraluminescent properties were obtained based on metal-enhanced fluorescence (MEF). Different silica spacers were synthesized to optimize the MEF enhancement factor (MEFEF). An optimal MEFEF was determined equal to 9.5 for shorter silica spacers (d-SiO2-=10 nm). These nanoparticles were deposed on Escherichia coli bacteria at different concentration levels for Bioimaging generation over their surfaces. The best luminescent nanoparticles were deposed on intermediate and higher bacteria concentrations. In the presence of intermediate bacteria concentrations, the ultraluminescent nanoparticles adsorbed showed an increase of 35% to 45% compared with individual nanoparticles. To modify the surface of individual bacteria, diluted samples of bacteria were used in which a 20% decrease in fluorescence emission was measured. In the presence of higher bacteria concentrations, fewer clear and bright images were obtained. At diluted ultraluminescent nanoparticle concentrations, a decrease in brightness and image detail was observed; and in the absence of nanoparticle deposition, no image was recorded. Accordingly, these ultraluminescent gold core-shell nanoparticles have been shown to be useful as platforms for biodetection and tracking applications.
Fil: Gontero, Daniela. Clínica de la Familia II; Argentina
Fil: Veglia, Alicia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Boudreau, Denis. Laval University; Canadá
Fil: Bracamonte, Angel Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia
BACTERIAL DETECTION
GOLD CORE-SHELL NANOPARTICLES
METAL-ENHANCED FLUORESCENCE
NANOIMAGING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/64370
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/64370
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Ultraluminescent gold core-shell nanoparticles applied to individual bacterial detection based on metal-enhanced fluorescence nanoimagingGontero, DanielaVeglia, Alicia VivianaBoudreau, DenisBracamonte, Angel GuillermoBACTERIAL DETECTIONGOLD CORE-SHELL NANOPARTICLESMETAL-ENHANCED FLUORESCENCENANOIMAGINGhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Gold core-shell nanoparticles were synthesized based on metallic cores, variable silica shell spacers covered with modified fluorescent silica layers. Ultraluminescent properties were obtained based on metal-enhanced fluorescence (MEF). Different silica spacers were synthesized to optimize the MEF enhancement factor (MEFEF). An optimal MEFEF was determined equal to 9.5 for shorter silica spacers (d-SiO2-=10 nm). These nanoparticles were deposed on Escherichia coli bacteria at different concentration levels for Bioimaging generation over their surfaces. The best luminescent nanoparticles were deposed on intermediate and higher bacteria concentrations. In the presence of intermediate bacteria concentrations, the ultraluminescent nanoparticles adsorbed showed an increase of 35% to 45% compared with individual nanoparticles. To modify the surface of individual bacteria, diluted samples of bacteria were used in which a 20% decrease in fluorescence emission was measured. In the presence of higher bacteria concentrations, fewer clear and bright images were obtained. At diluted ultraluminescent nanoparticle concentrations, a decrease in brightness and image detail was observed; and in the absence of nanoparticle deposition, no image was recorded. Accordingly, these ultraluminescent gold core-shell nanoparticles have been shown to be useful as platforms for biodetection and tracking applications.Fil: Gontero, Daniela. Clínica de la Familia II; ArgentinaFil: Veglia, Alicia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Boudreau, Denis. Laval University; CanadáFil: Bracamonte, Angel Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaSociety of Photo-Optical Instrumentation Engineers2018-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/64370Gontero, Daniela; Veglia, Alicia Viviana; Boudreau, Denis; Bracamonte, Angel Guillermo; Ultraluminescent gold core-shell nanoparticles applied to individual bacterial detection based on metal-enhanced fluorescence nanoimaging; Society of Photo-Optical Instrumentation Engineers; Journal Of Nanophotonics; 12; 1; 1-2018; 1-121934-26081934-2608CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://nanophotonics.spiedigitallibrary.org/article.aspx?doi=10.1117/1.JNP.12.012505info:eu-repo/semantics/altIdentifier/doi/10.1117/1.JNP.12.012505info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:48Zoai:ri.conicet.gov.ar:11336/64370instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:49.107CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ultraluminescent gold core-shell nanoparticles applied to individual bacterial detection based on metal-enhanced fluorescence nanoimaging
title Ultraluminescent gold core-shell nanoparticles applied to individual bacterial detection based on metal-enhanced fluorescence nanoimaging
spellingShingle Ultraluminescent gold core-shell nanoparticles applied to individual bacterial detection based on metal-enhanced fluorescence nanoimaging
Gontero, Daniela
BACTERIAL DETECTION
GOLD CORE-SHELL NANOPARTICLES
METAL-ENHANCED FLUORESCENCE
NANOIMAGING
title_short Ultraluminescent gold core-shell nanoparticles applied to individual bacterial detection based on metal-enhanced fluorescence nanoimaging
title_full Ultraluminescent gold core-shell nanoparticles applied to individual bacterial detection based on metal-enhanced fluorescence nanoimaging
title_fullStr Ultraluminescent gold core-shell nanoparticles applied to individual bacterial detection based on metal-enhanced fluorescence nanoimaging
title_full_unstemmed Ultraluminescent gold core-shell nanoparticles applied to individual bacterial detection based on metal-enhanced fluorescence nanoimaging
title_sort Ultraluminescent gold core-shell nanoparticles applied to individual bacterial detection based on metal-enhanced fluorescence nanoimaging
dc.creator.none.fl_str_mv Gontero, Daniela
Veglia, Alicia Viviana
Boudreau, Denis
Bracamonte, Angel Guillermo
author Gontero, Daniela
author_facet Gontero, Daniela
Veglia, Alicia Viviana
Boudreau, Denis
Bracamonte, Angel Guillermo
author_role author
author2 Veglia, Alicia Viviana
Boudreau, Denis
Bracamonte, Angel Guillermo
author2_role author
author
author
dc.subject.none.fl_str_mv BACTERIAL DETECTION
GOLD CORE-SHELL NANOPARTICLES
METAL-ENHANCED FLUORESCENCE
NANOIMAGING
topic BACTERIAL DETECTION
GOLD CORE-SHELL NANOPARTICLES
METAL-ENHANCED FLUORESCENCE
NANOIMAGING
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Gold core-shell nanoparticles were synthesized based on metallic cores, variable silica shell spacers covered with modified fluorescent silica layers. Ultraluminescent properties were obtained based on metal-enhanced fluorescence (MEF). Different silica spacers were synthesized to optimize the MEF enhancement factor (MEFEF). An optimal MEFEF was determined equal to 9.5 for shorter silica spacers (d-SiO2-=10 nm). These nanoparticles were deposed on Escherichia coli bacteria at different concentration levels for Bioimaging generation over their surfaces. The best luminescent nanoparticles were deposed on intermediate and higher bacteria concentrations. In the presence of intermediate bacteria concentrations, the ultraluminescent nanoparticles adsorbed showed an increase of 35% to 45% compared with individual nanoparticles. To modify the surface of individual bacteria, diluted samples of bacteria were used in which a 20% decrease in fluorescence emission was measured. In the presence of higher bacteria concentrations, fewer clear and bright images were obtained. At diluted ultraluminescent nanoparticle concentrations, a decrease in brightness and image detail was observed; and in the absence of nanoparticle deposition, no image was recorded. Accordingly, these ultraluminescent gold core-shell nanoparticles have been shown to be useful as platforms for biodetection and tracking applications.
Fil: Gontero, Daniela. Clínica de la Familia II; Argentina
Fil: Veglia, Alicia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Boudreau, Denis. Laval University; Canadá
Fil: Bracamonte, Angel Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description Gold core-shell nanoparticles were synthesized based on metallic cores, variable silica shell spacers covered with modified fluorescent silica layers. Ultraluminescent properties were obtained based on metal-enhanced fluorescence (MEF). Different silica spacers were synthesized to optimize the MEF enhancement factor (MEFEF). An optimal MEFEF was determined equal to 9.5 for shorter silica spacers (d-SiO2-=10 nm). These nanoparticles were deposed on Escherichia coli bacteria at different concentration levels for Bioimaging generation over their surfaces. The best luminescent nanoparticles were deposed on intermediate and higher bacteria concentrations. In the presence of intermediate bacteria concentrations, the ultraluminescent nanoparticles adsorbed showed an increase of 35% to 45% compared with individual nanoparticles. To modify the surface of individual bacteria, diluted samples of bacteria were used in which a 20% decrease in fluorescence emission was measured. In the presence of higher bacteria concentrations, fewer clear and bright images were obtained. At diluted ultraluminescent nanoparticle concentrations, a decrease in brightness and image detail was observed; and in the absence of nanoparticle deposition, no image was recorded. Accordingly, these ultraluminescent gold core-shell nanoparticles have been shown to be useful as platforms for biodetection and tracking applications.
publishDate 2018
dc.date.none.fl_str_mv 2018-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/64370
Gontero, Daniela; Veglia, Alicia Viviana; Boudreau, Denis; Bracamonte, Angel Guillermo; Ultraluminescent gold core-shell nanoparticles applied to individual bacterial detection based on metal-enhanced fluorescence nanoimaging; Society of Photo-Optical Instrumentation Engineers; Journal Of Nanophotonics; 12; 1; 1-2018; 1-12
1934-2608
1934-2608
CONICET Digital
CONICET
url http://hdl.handle.net/11336/64370
identifier_str_mv Gontero, Daniela; Veglia, Alicia Viviana; Boudreau, Denis; Bracamonte, Angel Guillermo; Ultraluminescent gold core-shell nanoparticles applied to individual bacterial detection based on metal-enhanced fluorescence nanoimaging; Society of Photo-Optical Instrumentation Engineers; Journal Of Nanophotonics; 12; 1; 1-2018; 1-12
1934-2608
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://nanophotonics.spiedigitallibrary.org/article.aspx?doi=10.1117/1.JNP.12.012505
info:eu-repo/semantics/altIdentifier/doi/10.1117/1.JNP.12.012505
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society of Photo-Optical Instrumentation Engineers
publisher.none.fl_str_mv Society of Photo-Optical Instrumentation Engineers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269719873191936
score 13.13397

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