HLA-G1 increases the radiosensitivity of human tumoral cells

Autores
Gallegos, Cristina Eugenia; Michelin, Severino; Baffa Trasci, Sofía; Aballay Lobos, Elizabeth; Dubner, Diana; Carosella, Edgardo D.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Different molecules regulate the response of tumoral tissues to ionizing radiation. The objective of this work was to determine if HLA-G1 expression modulates the radiosensitivity of human tumoral cell lines. To this end, human melanoma M8 and human erythroleukemia K562 cell lines, with their correspondent HLA-G1 negative and positive variants, were gamma irradiated and the survival frequency was determined by clonogenic assay. The survival fraction of HLA-G1 expressing cells was around 60% of HLA-G1 negative cells. The generation of acidic vesicular organelles was higher in HLA-G1 positive cells. Apoptosis levels showed statistically significant differences only in K562 cells, whereas the variation in G2/M cycle progression was only significant in M8 cells. In addition, irradiation diminished cell-surface HLA-G1 and increased soluble HLA-G1 levels. Soluble HLA-G1 has no influence on cell survival in any cell line. In summary, we could demonstrate that HLA-G1 confers higher radiosensitivity to HLA-G1 expressing cells.
Fil: Gallegos, Cristina Eugenia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico Bahia Blanca. Instituto de Ciencias Biologicas y Biomedicas del Sur; Argentina. Universidad Nacional del Sur; Argentina. Autoridad Regulatoria Nuclear; Argentina
Fil: Michelin, Severino. Autoridad Regulatoria Nuclear; Argentina
Fil: Baffa Trasci, Sofía. Autoridad Regulatoria Nuclear; Argentina
Fil: Aballay Lobos, Elizabeth. Autoridad Regulatoria Nuclear; Argentina
Fil: Dubner, Diana. Autoridad Regulatoria Nuclear; Argentina
Fil: Carosella, Edgardo D.. University Paris Diderot; Francia. Commissariat a l’Energie Atomique et aux Energies Alternatives. Institute of Emerging Diseases and Innovative Therapies. Research Division in Hematology and Immunology; Francia
Materia
Hla-G1
Ionizing Radiation
Radiosensitivity
Tumoral Cells
Melanoma Cells
Erythroleukemia Cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/6356
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling HLA-G1 increases the radiosensitivity of human tumoral cellsGallegos, Cristina EugeniaMichelin, SeverinoBaffa Trasci, SofíaAballay Lobos, ElizabethDubner, DianaCarosella, Edgardo D.Hla-G1Ionizing RadiationRadiosensitivityTumoral CellsMelanoma CellsErythroleukemia Cellshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Different molecules regulate the response of tumoral tissues to ionizing radiation. The objective of this work was to determine if HLA-G1 expression modulates the radiosensitivity of human tumoral cell lines. To this end, human melanoma M8 and human erythroleukemia K562 cell lines, with their correspondent HLA-G1 negative and positive variants, were gamma irradiated and the survival frequency was determined by clonogenic assay. The survival fraction of HLA-G1 expressing cells was around 60% of HLA-G1 negative cells. The generation of acidic vesicular organelles was higher in HLA-G1 positive cells. Apoptosis levels showed statistically significant differences only in K562 cells, whereas the variation in G2/M cycle progression was only significant in M8 cells. In addition, irradiation diminished cell-surface HLA-G1 and increased soluble HLA-G1 levels. Soluble HLA-G1 has no influence on cell survival in any cell line. In summary, we could demonstrate that HLA-G1 confers higher radiosensitivity to HLA-G1 expressing cells.Fil: Gallegos, Cristina Eugenia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico Bahia Blanca. Instituto de Ciencias Biologicas y Biomedicas del Sur; Argentina. Universidad Nacional del Sur; Argentina. Autoridad Regulatoria Nuclear; ArgentinaFil: Michelin, Severino. Autoridad Regulatoria Nuclear; ArgentinaFil: Baffa Trasci, Sofía. Autoridad Regulatoria Nuclear; ArgentinaFil: Aballay Lobos, Elizabeth. Autoridad Regulatoria Nuclear; ArgentinaFil: Dubner, Diana. Autoridad Regulatoria Nuclear; ArgentinaFil: Carosella, Edgardo D.. University Paris Diderot; Francia. Commissariat a l’Energie Atomique et aux Energies Alternatives. Institute of Emerging Diseases and Innovative Therapies. Research Division in Hematology and Immunology; FranciaElsevier2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6356Gallegos, Cristina Eugenia; Michelin, Severino; Baffa Trasci, Sofía; Aballay Lobos, Elizabeth; Dubner, Diana; et al.; HLA-G1 increases the radiosensitivity of human tumoral cells; Elsevier; Cellular Immunology; 287; 2; 2-2014; 106-1110008-8749enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.cellimm.2014.01.005info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/pmid/24487034info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0008874914000148info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:44:20Zoai:ri.conicet.gov.ar:11336/6356instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:44:20.429CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv HLA-G1 increases the radiosensitivity of human tumoral cells
title HLA-G1 increases the radiosensitivity of human tumoral cells
spellingShingle HLA-G1 increases the radiosensitivity of human tumoral cells
Gallegos, Cristina Eugenia
Hla-G1
Ionizing Radiation
Radiosensitivity
Tumoral Cells
Melanoma Cells
Erythroleukemia Cells
title_short HLA-G1 increases the radiosensitivity of human tumoral cells
title_full HLA-G1 increases the radiosensitivity of human tumoral cells
title_fullStr HLA-G1 increases the radiosensitivity of human tumoral cells
title_full_unstemmed HLA-G1 increases the radiosensitivity of human tumoral cells
title_sort HLA-G1 increases the radiosensitivity of human tumoral cells
dc.creator.none.fl_str_mv Gallegos, Cristina Eugenia
Michelin, Severino
Baffa Trasci, Sofía
Aballay Lobos, Elizabeth
Dubner, Diana
Carosella, Edgardo D.
author Gallegos, Cristina Eugenia
author_facet Gallegos, Cristina Eugenia
Michelin, Severino
Baffa Trasci, Sofía
Aballay Lobos, Elizabeth
Dubner, Diana
Carosella, Edgardo D.
author_role author
author2 Michelin, Severino
Baffa Trasci, Sofía
Aballay Lobos, Elizabeth
Dubner, Diana
Carosella, Edgardo D.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Hla-G1
Ionizing Radiation
Radiosensitivity
Tumoral Cells
Melanoma Cells
Erythroleukemia Cells
topic Hla-G1
Ionizing Radiation
Radiosensitivity
Tumoral Cells
Melanoma Cells
Erythroleukemia Cells
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Different molecules regulate the response of tumoral tissues to ionizing radiation. The objective of this work was to determine if HLA-G1 expression modulates the radiosensitivity of human tumoral cell lines. To this end, human melanoma M8 and human erythroleukemia K562 cell lines, with their correspondent HLA-G1 negative and positive variants, were gamma irradiated and the survival frequency was determined by clonogenic assay. The survival fraction of HLA-G1 expressing cells was around 60% of HLA-G1 negative cells. The generation of acidic vesicular organelles was higher in HLA-G1 positive cells. Apoptosis levels showed statistically significant differences only in K562 cells, whereas the variation in G2/M cycle progression was only significant in M8 cells. In addition, irradiation diminished cell-surface HLA-G1 and increased soluble HLA-G1 levels. Soluble HLA-G1 has no influence on cell survival in any cell line. In summary, we could demonstrate that HLA-G1 confers higher radiosensitivity to HLA-G1 expressing cells.
Fil: Gallegos, Cristina Eugenia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico Bahia Blanca. Instituto de Ciencias Biologicas y Biomedicas del Sur; Argentina. Universidad Nacional del Sur; Argentina. Autoridad Regulatoria Nuclear; Argentina
Fil: Michelin, Severino. Autoridad Regulatoria Nuclear; Argentina
Fil: Baffa Trasci, Sofía. Autoridad Regulatoria Nuclear; Argentina
Fil: Aballay Lobos, Elizabeth. Autoridad Regulatoria Nuclear; Argentina
Fil: Dubner, Diana. Autoridad Regulatoria Nuclear; Argentina
Fil: Carosella, Edgardo D.. University Paris Diderot; Francia. Commissariat a l’Energie Atomique et aux Energies Alternatives. Institute of Emerging Diseases and Innovative Therapies. Research Division in Hematology and Immunology; Francia
description Different molecules regulate the response of tumoral tissues to ionizing radiation. The objective of this work was to determine if HLA-G1 expression modulates the radiosensitivity of human tumoral cell lines. To this end, human melanoma M8 and human erythroleukemia K562 cell lines, with their correspondent HLA-G1 negative and positive variants, were gamma irradiated and the survival frequency was determined by clonogenic assay. The survival fraction of HLA-G1 expressing cells was around 60% of HLA-G1 negative cells. The generation of acidic vesicular organelles was higher in HLA-G1 positive cells. Apoptosis levels showed statistically significant differences only in K562 cells, whereas the variation in G2/M cycle progression was only significant in M8 cells. In addition, irradiation diminished cell-surface HLA-G1 and increased soluble HLA-G1 levels. Soluble HLA-G1 has no influence on cell survival in any cell line. In summary, we could demonstrate that HLA-G1 confers higher radiosensitivity to HLA-G1 expressing cells.
publishDate 2014
dc.date.none.fl_str_mv 2014-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/6356
Gallegos, Cristina Eugenia; Michelin, Severino; Baffa Trasci, Sofía; Aballay Lobos, Elizabeth; Dubner, Diana; et al.; HLA-G1 increases the radiosensitivity of human tumoral cells; Elsevier; Cellular Immunology; 287; 2; 2-2014; 106-111
0008-8749
url http://hdl.handle.net/11336/6356
identifier_str_mv Gallegos, Cristina Eugenia; Michelin, Severino; Baffa Trasci, Sofía; Aballay Lobos, Elizabeth; Dubner, Diana; et al.; HLA-G1 increases the radiosensitivity of human tumoral cells; Elsevier; Cellular Immunology; 287; 2; 2-2014; 106-111
0008-8749
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cellimm.2014.01.005
info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/pmid/24487034
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0008874914000148
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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