L-arginine supplementation reduces mortality and improves disease outcome in mice infected with Trypanosoma cruzi

Autores
Carbajosa, Sofía; Rodríguez Angulo, Héctor O.; Gea, Susana; Chillón Marinas, Carlos; Poveda, Cristina; Maza, María C.; Colombet, Diana; Fresno, Manuel; Girones Pujol, Nuria
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chagas disease caused by Trypanosoma cruzi is a neglected disease that affects about 7 million people in Latin America, recently emerging on other continents due to migration. As infection in mice is characterized by depletion of plasma L-arginine, the effect on infection outcome was tested in mice with or without L-arginine supplementation and treatment with 1400W, a specific inhibitor of inducible nitric oxide synthase (iNOS). We found that levels of L-arginine and citrulline were reduced in the heart and plasma of infected mice, whereas levels of asymmetric dimethylarginine, an endogenous iNOS inhibitor, were higher. Moreover, L-arginine supplementation decreased parasitemia and heart parasite burden, improving clinical score and survival. Nitric oxide production in heart tissue and plasma was increased by L-arginine supplementation, while pharmacological inhibition of iNOS yielded an increase in parasitemia and worse clinical score. Interestingly, electrocardiograms improved in mice supplemented with L-arginine, suggesting that it modulates infection and heart function and is thus a potential biomarker of pathology. More importantly, L-arginine may be useful for treating T. cruzi infection, either alone or in combination with other antiparasitic drugs.
Fil: Carbajosa, Sofía. Consejo Superior de Investigaciones Científicas; España. Universidad Autónoma de Madrid; España
Fil: Rodríguez Angulo, Héctor O.. Instituto Venezolano de Investigaciones Científicas; Venezuela
Fil: Gea, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Chillón Marinas, Carlos. Universidad Autónoma de Madrid; España. Consejo Superior de Investigaciones Científicas; España
Fil: Poveda, Cristina. Universidad Autónoma de Madrid; España. Consejo Superior de Investigaciones Científicas; España
Fil: Maza, María C.. Consejo Superior de Investigaciones Científicas; España. Universidad Autonoma de Madrid. Hospital Universitario de la Princesa; España. Universidad Autónoma de Madrid; España
Fil: Colombet, Diana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Fresno, Manuel. Universidad Autonoma de Madrid. Hospital Universitario de la Princesa; España. Universidad Autónoma de Madrid; España. Consejo Superior de Investigaciones Científicas; España
Fil: Girones Pujol, Nuria. Consejo Superior de Investigaciones Científicas; España. Universidad Autonoma de Madrid. Hospital Universitario de la Princesa; España. Universidad Autónoma de Madrid; España
Materia
Trypanosoma cruzi
L-arginina
Chagas Disease
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/95904
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/95904
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling L-arginine supplementation reduces mortality and improves disease outcome in mice infected with Trypanosoma cruziCarbajosa, SofíaRodríguez Angulo, Héctor O.Gea, SusanaChillón Marinas, CarlosPoveda, CristinaMaza, María C.Colombet, DianaFresno, ManuelGirones Pujol, NuriaTrypanosoma cruziL-argininaChagas Diseasehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Chagas disease caused by Trypanosoma cruzi is a neglected disease that affects about 7 million people in Latin America, recently emerging on other continents due to migration. As infection in mice is characterized by depletion of plasma L-arginine, the effect on infection outcome was tested in mice with or without L-arginine supplementation and treatment with 1400W, a specific inhibitor of inducible nitric oxide synthase (iNOS). We found that levels of L-arginine and citrulline were reduced in the heart and plasma of infected mice, whereas levels of asymmetric dimethylarginine, an endogenous iNOS inhibitor, were higher. Moreover, L-arginine supplementation decreased parasitemia and heart parasite burden, improving clinical score and survival. Nitric oxide production in heart tissue and plasma was increased by L-arginine supplementation, while pharmacological inhibition of iNOS yielded an increase in parasitemia and worse clinical score. Interestingly, electrocardiograms improved in mice supplemented with L-arginine, suggesting that it modulates infection and heart function and is thus a potential biomarker of pathology. More importantly, L-arginine may be useful for treating T. cruzi infection, either alone or in combination with other antiparasitic drugs.Fil: Carbajosa, Sofía. Consejo Superior de Investigaciones Científicas; España. Universidad Autónoma de Madrid; EspañaFil: Rodríguez Angulo, Héctor O.. Instituto Venezolano de Investigaciones Científicas; VenezuelaFil: Gea, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Chillón Marinas, Carlos. Universidad Autónoma de Madrid; España. Consejo Superior de Investigaciones Científicas; EspañaFil: Poveda, Cristina. Universidad Autónoma de Madrid; España. Consejo Superior de Investigaciones Científicas; EspañaFil: Maza, María C.. Consejo Superior de Investigaciones Científicas; España. Universidad Autonoma de Madrid. Hospital Universitario de la Princesa; España. Universidad Autónoma de Madrid; EspañaFil: Colombet, Diana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Fresno, Manuel. Universidad Autonoma de Madrid. Hospital Universitario de la Princesa; España. Universidad Autónoma de Madrid; España. Consejo Superior de Investigaciones Científicas; EspañaFil: Girones Pujol, Nuria. Consejo Superior de Investigaciones Científicas; España. Universidad Autonoma de Madrid. Hospital Universitario de la Princesa; España. Universidad Autónoma de Madrid; EspañaPublic Library of Science2018-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/95904Carbajosa, Sofía; Rodríguez Angulo, Héctor O.; Gea, Susana; Chillón Marinas, Carlos; Poveda, Cristina; et al.; L-arginine supplementation reduces mortality and improves disease outcome in mice infected with Trypanosoma cruzi; Public Library of Science; PLoS Neglected Tropical Diseases; 12; 1; 1-2018; 1-161935-27271935-2735CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0006179info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0006179info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:17Zoai:ri.conicet.gov.ar:11336/95904instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:17.949CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv L-arginine supplementation reduces mortality and improves disease outcome in mice infected with Trypanosoma cruzi
title L-arginine supplementation reduces mortality and improves disease outcome in mice infected with Trypanosoma cruzi
spellingShingle L-arginine supplementation reduces mortality and improves disease outcome in mice infected with Trypanosoma cruzi
Carbajosa, Sofía
Trypanosoma cruzi
L-arginina
Chagas Disease
title_short L-arginine supplementation reduces mortality and improves disease outcome in mice infected with Trypanosoma cruzi
title_full L-arginine supplementation reduces mortality and improves disease outcome in mice infected with Trypanosoma cruzi
title_fullStr L-arginine supplementation reduces mortality and improves disease outcome in mice infected with Trypanosoma cruzi
title_full_unstemmed L-arginine supplementation reduces mortality and improves disease outcome in mice infected with Trypanosoma cruzi
title_sort L-arginine supplementation reduces mortality and improves disease outcome in mice infected with Trypanosoma cruzi
dc.creator.none.fl_str_mv Carbajosa, Sofía
Rodríguez Angulo, Héctor O.
Gea, Susana
Chillón Marinas, Carlos
Poveda, Cristina
Maza, María C.
Colombet, Diana
Fresno, Manuel
Girones Pujol, Nuria
author Carbajosa, Sofía
author_facet Carbajosa, Sofía
Rodríguez Angulo, Héctor O.
Gea, Susana
Chillón Marinas, Carlos
Poveda, Cristina
Maza, María C.
Colombet, Diana
Fresno, Manuel
Girones Pujol, Nuria
author_role author
author2 Rodríguez Angulo, Héctor O.
Gea, Susana
Chillón Marinas, Carlos
Poveda, Cristina
Maza, María C.
Colombet, Diana
Fresno, Manuel
Girones Pujol, Nuria
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Trypanosoma cruzi
L-arginina
Chagas Disease
topic Trypanosoma cruzi
L-arginina
Chagas Disease
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Chagas disease caused by Trypanosoma cruzi is a neglected disease that affects about 7 million people in Latin America, recently emerging on other continents due to migration. As infection in mice is characterized by depletion of plasma L-arginine, the effect on infection outcome was tested in mice with or without L-arginine supplementation and treatment with 1400W, a specific inhibitor of inducible nitric oxide synthase (iNOS). We found that levels of L-arginine and citrulline were reduced in the heart and plasma of infected mice, whereas levels of asymmetric dimethylarginine, an endogenous iNOS inhibitor, were higher. Moreover, L-arginine supplementation decreased parasitemia and heart parasite burden, improving clinical score and survival. Nitric oxide production in heart tissue and plasma was increased by L-arginine supplementation, while pharmacological inhibition of iNOS yielded an increase in parasitemia and worse clinical score. Interestingly, electrocardiograms improved in mice supplemented with L-arginine, suggesting that it modulates infection and heart function and is thus a potential biomarker of pathology. More importantly, L-arginine may be useful for treating T. cruzi infection, either alone or in combination with other antiparasitic drugs.
Fil: Carbajosa, Sofía. Consejo Superior de Investigaciones Científicas; España. Universidad Autónoma de Madrid; España
Fil: Rodríguez Angulo, Héctor O.. Instituto Venezolano de Investigaciones Científicas; Venezuela
Fil: Gea, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Chillón Marinas, Carlos. Universidad Autónoma de Madrid; España. Consejo Superior de Investigaciones Científicas; España
Fil: Poveda, Cristina. Universidad Autónoma de Madrid; España. Consejo Superior de Investigaciones Científicas; España
Fil: Maza, María C.. Consejo Superior de Investigaciones Científicas; España. Universidad Autonoma de Madrid. Hospital Universitario de la Princesa; España. Universidad Autónoma de Madrid; España
Fil: Colombet, Diana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Fresno, Manuel. Universidad Autonoma de Madrid. Hospital Universitario de la Princesa; España. Universidad Autónoma de Madrid; España. Consejo Superior de Investigaciones Científicas; España
Fil: Girones Pujol, Nuria. Consejo Superior de Investigaciones Científicas; España. Universidad Autonoma de Madrid. Hospital Universitario de la Princesa; España. Universidad Autónoma de Madrid; España
description Chagas disease caused by Trypanosoma cruzi is a neglected disease that affects about 7 million people in Latin America, recently emerging on other continents due to migration. As infection in mice is characterized by depletion of plasma L-arginine, the effect on infection outcome was tested in mice with or without L-arginine supplementation and treatment with 1400W, a specific inhibitor of inducible nitric oxide synthase (iNOS). We found that levels of L-arginine and citrulline were reduced in the heart and plasma of infected mice, whereas levels of asymmetric dimethylarginine, an endogenous iNOS inhibitor, were higher. Moreover, L-arginine supplementation decreased parasitemia and heart parasite burden, improving clinical score and survival. Nitric oxide production in heart tissue and plasma was increased by L-arginine supplementation, while pharmacological inhibition of iNOS yielded an increase in parasitemia and worse clinical score. Interestingly, electrocardiograms improved in mice supplemented with L-arginine, suggesting that it modulates infection and heart function and is thus a potential biomarker of pathology. More importantly, L-arginine may be useful for treating T. cruzi infection, either alone or in combination with other antiparasitic drugs.
publishDate 2018
dc.date.none.fl_str_mv 2018-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/95904
Carbajosa, Sofía; Rodríguez Angulo, Héctor O.; Gea, Susana; Chillón Marinas, Carlos; Poveda, Cristina; et al.; L-arginine supplementation reduces mortality and improves disease outcome in mice infected with Trypanosoma cruzi; Public Library of Science; PLoS Neglected Tropical Diseases; 12; 1; 1-2018; 1-16
1935-2727
1935-2735
CONICET Digital
CONICET
url http://hdl.handle.net/11336/95904
identifier_str_mv Carbajosa, Sofía; Rodríguez Angulo, Héctor O.; Gea, Susana; Chillón Marinas, Carlos; Poveda, Cristina; et al.; L-arginine supplementation reduces mortality and improves disease outcome in mice infected with Trypanosoma cruzi; Public Library of Science; PLoS Neglected Tropical Diseases; 12; 1; 1-2018; 1-16
1935-2727
1935-2735
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0006179
info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0006179
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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