Biochemical characterization of a low-affinity arginine permease from the parasite Trypanosoma cruzi

Autores
Canepa, Gaspar Exequiel; Silber, Ariel; Bouvier, León Alberto; Pereira, Claudio Alejandro
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Trypanosoma cruzi, the etiological agent of Chagas disease, uses arginine for several metabolic processes, including energy reserves management. In the present work, a novel low-affinity arginine transport system has been studied. Maximum velocity (97 pmol min−1 per 107 cells), and an estimate for the apparent Km value (350 ìM) of this arginine transporter, were 6-fold and 80-fold higher respectively, when compared with the previously described high-affinity arginine transport system. This transport activity seems to be H+-mediated, presents a broad specificity by other amino acids such as methionine, and is regulated along the parasite growth curve and life cycle.
Fil: Canepa, Gaspar Exequiel. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Silber, Ariel. Universidade de Sao Paulo; Brasil
Fil: Bouvier, León Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Pereira, Claudio Alejandro. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Universidade de Sao Paulo; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Trypanosoma cruzi
Metabolite transport
Arginine
Aminoacid transporter
Chagas disease
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/110035
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Biochemical characterization of a low-affinity arginine permease from the parasite Trypanosoma cruziCanepa, Gaspar ExequielSilber, ArielBouvier, León AlbertoPereira, Claudio AlejandroTrypanosoma cruziMetabolite transportArginineAminoacid transporterChagas diseasehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Trypanosoma cruzi, the etiological agent of Chagas disease, uses arginine for several metabolic processes, including energy reserves management. In the present work, a novel low-affinity arginine transport system has been studied. Maximum velocity (97 pmol min−1 per 107 cells), and an estimate for the apparent Km value (350 ìM) of this arginine transporter, were 6-fold and 80-fold higher respectively, when compared with the previously described high-affinity arginine transport system. This transport activity seems to be H+-mediated, presents a broad specificity by other amino acids such as methionine, and is regulated along the parasite growth curve and life cycle.Fil: Canepa, Gaspar Exequiel. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Silber, Ariel. Universidade de Sao Paulo; BrasilFil: Bouvier, León Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Pereira, Claudio Alejandro. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Universidade de Sao Paulo; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley Blackwell Publishing, Inc2004-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/110035Canepa, Gaspar Exequiel; Silber, Ariel; Bouvier, León Alberto; Pereira, Claudio Alejandro; Biochemical characterization of a low-affinity arginine permease from the parasite Trypanosoma cruzi; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 236; 1; 7-2004; 79-840378-1097CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1574-6968.2004.tb09630.x?sid=nlm%3Apubmedinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6968.2004.tb09630.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:38Zoai:ri.conicet.gov.ar:11336/110035instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:38.57CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biochemical characterization of a low-affinity arginine permease from the parasite Trypanosoma cruzi
title Biochemical characterization of a low-affinity arginine permease from the parasite Trypanosoma cruzi
spellingShingle Biochemical characterization of a low-affinity arginine permease from the parasite Trypanosoma cruzi
Canepa, Gaspar Exequiel
Trypanosoma cruzi
Metabolite transport
Arginine
Aminoacid transporter
Chagas disease
title_short Biochemical characterization of a low-affinity arginine permease from the parasite Trypanosoma cruzi
title_full Biochemical characterization of a low-affinity arginine permease from the parasite Trypanosoma cruzi
title_fullStr Biochemical characterization of a low-affinity arginine permease from the parasite Trypanosoma cruzi
title_full_unstemmed Biochemical characterization of a low-affinity arginine permease from the parasite Trypanosoma cruzi
title_sort Biochemical characterization of a low-affinity arginine permease from the parasite Trypanosoma cruzi
dc.creator.none.fl_str_mv Canepa, Gaspar Exequiel
Silber, Ariel
Bouvier, León Alberto
Pereira, Claudio Alejandro
author Canepa, Gaspar Exequiel
author_facet Canepa, Gaspar Exequiel
Silber, Ariel
Bouvier, León Alberto
Pereira, Claudio Alejandro
author_role author
author2 Silber, Ariel
Bouvier, León Alberto
Pereira, Claudio Alejandro
author2_role author
author
author
dc.subject.none.fl_str_mv Trypanosoma cruzi
Metabolite transport
Arginine
Aminoacid transporter
Chagas disease
topic Trypanosoma cruzi
Metabolite transport
Arginine
Aminoacid transporter
Chagas disease
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Trypanosoma cruzi, the etiological agent of Chagas disease, uses arginine for several metabolic processes, including energy reserves management. In the present work, a novel low-affinity arginine transport system has been studied. Maximum velocity (97 pmol min−1 per 107 cells), and an estimate for the apparent Km value (350 ìM) of this arginine transporter, were 6-fold and 80-fold higher respectively, when compared with the previously described high-affinity arginine transport system. This transport activity seems to be H+-mediated, presents a broad specificity by other amino acids such as methionine, and is regulated along the parasite growth curve and life cycle.
Fil: Canepa, Gaspar Exequiel. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Silber, Ariel. Universidade de Sao Paulo; Brasil
Fil: Bouvier, León Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Pereira, Claudio Alejandro. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Universidade de Sao Paulo; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Trypanosoma cruzi, the etiological agent of Chagas disease, uses arginine for several metabolic processes, including energy reserves management. In the present work, a novel low-affinity arginine transport system has been studied. Maximum velocity (97 pmol min−1 per 107 cells), and an estimate for the apparent Km value (350 ìM) of this arginine transporter, were 6-fold and 80-fold higher respectively, when compared with the previously described high-affinity arginine transport system. This transport activity seems to be H+-mediated, presents a broad specificity by other amino acids such as methionine, and is regulated along the parasite growth curve and life cycle.
publishDate 2004
dc.date.none.fl_str_mv 2004-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/110035
Canepa, Gaspar Exequiel; Silber, Ariel; Bouvier, León Alberto; Pereira, Claudio Alejandro; Biochemical characterization of a low-affinity arginine permease from the parasite Trypanosoma cruzi; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 236; 1; 7-2004; 79-84
0378-1097
CONICET Digital
CONICET
url http://hdl.handle.net/11336/110035
identifier_str_mv Canepa, Gaspar Exequiel; Silber, Ariel; Bouvier, León Alberto; Pereira, Claudio Alejandro; Biochemical characterization of a low-affinity arginine permease from the parasite Trypanosoma cruzi; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 236; 1; 7-2004; 79-84
0378-1097
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1574-6968.2004.tb09630.x?sid=nlm%3Apubmed
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6968.2004.tb09630.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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