In vitro antistaphylococcal effects of a novel 45S5 bioglass/agar-gelatin biocomposite films

Autores
Rivadeneira, Josefina; Audisio, Marcela Carina; Boccaccini, Aldo R.; Gorustovich Alonso, Alejandro Adrian
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims: To assess the antibacterial efficacy of new composite materials developed from microparticles of 45S5 bioactive glass (BG) and agar-gelatin films. Methods and Results: In vitro antibacterial activity was evaluated against Staphylococcus spp. because of the importance of this pathogen in damaged tissues and in failures associated with biomaterial implants. To our knowledge, this is the first paper reporting on the suitable combination of BG and agar-gelatin for bioactive and antibacterial films. Bacterial suspensions up or below 105 CFU ml-1 reflecting situations of wound infection and of noninfection, respectively, were prepared and then put in contact with the biomaterials at 37°C. After 24 and 48 h of incubation, the pH value was measured and the staphylococci strains viability was determined by counting in Mueller-Hinton agar plates. Moreover, the biomaterials were prepared for observation under scanning electron microscopy (SEM). Biocomposites (BCs) showed a strong antibacterial effect against all staphylococci strains tested. Some differences were found depending on the strain, the inoculum size and the contact time. This effect was correlated with an alkalinization of the media. By SEM analyses, no bacterial presence was observed on the surface of BCs in any of the cell concentrations tested at any time. Conclusions: Overall, the coating of 45S5 BG on agar-gelatin films promoted BCs with strong antistaphylococcal activity. The effect was efficient under bacterial concentration up or below 105 CFU ml-1. Additionally, none of the strains were found on BCs surfaces. Significance and Impact of Study: 45S5 bioglass/agar-gelatin biocomposite films are reported for the first time. The results suggest a potential application as wound dressing.
Fil: Rivadeneira, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; Argentina
Fil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Boccaccini, Aldo R.. Universitat Erlangen-Nuremberg; Alemania
Fil: Gorustovich Alonso, Alejandro Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; Argentina
Materia
Antibacterial Activity
Bioactive Glass
Biocomposites
Biopolymers
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/77809
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/77809
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro antistaphylococcal effects of a novel 45S5 bioglass/agar-gelatin biocomposite filmsRivadeneira, JosefinaAudisio, Marcela CarinaBoccaccini, Aldo R.Gorustovich Alonso, Alejandro AdrianAntibacterial ActivityBioactive GlassBiocompositesBiopolymershttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Aims: To assess the antibacterial efficacy of new composite materials developed from microparticles of 45S5 bioactive glass (BG) and agar-gelatin films. Methods and Results: In vitro antibacterial activity was evaluated against Staphylococcus spp. because of the importance of this pathogen in damaged tissues and in failures associated with biomaterial implants. To our knowledge, this is the first paper reporting on the suitable combination of BG and agar-gelatin for bioactive and antibacterial films. Bacterial suspensions up or below 105 CFU ml-1 reflecting situations of wound infection and of noninfection, respectively, were prepared and then put in contact with the biomaterials at 37°C. After 24 and 48 h of incubation, the pH value was measured and the staphylococci strains viability was determined by counting in Mueller-Hinton agar plates. Moreover, the biomaterials were prepared for observation under scanning electron microscopy (SEM). Biocomposites (BCs) showed a strong antibacterial effect against all staphylococci strains tested. Some differences were found depending on the strain, the inoculum size and the contact time. This effect was correlated with an alkalinization of the media. By SEM analyses, no bacterial presence was observed on the surface of BCs in any of the cell concentrations tested at any time. Conclusions: Overall, the coating of 45S5 BG on agar-gelatin films promoted BCs with strong antistaphylococcal activity. The effect was efficient under bacterial concentration up or below 105 CFU ml-1. Additionally, none of the strains were found on BCs surfaces. Significance and Impact of Study: 45S5 bioglass/agar-gelatin biocomposite films are reported for the first time. The results suggest a potential application as wound dressing.Fil: Rivadeneira, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; ArgentinaFil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; ArgentinaFil: Boccaccini, Aldo R.. Universitat Erlangen-Nuremberg; AlemaniaFil: Gorustovich Alonso, Alejandro Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; ArgentinaWiley Blackwell Publishing, Inc2013-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/77809Rivadeneira, Josefina; Audisio, Marcela Carina; Boccaccini, Aldo R.; Gorustovich Alonso, Alejandro Adrian; In vitro antistaphylococcal effects of a novel 45S5 bioglass/agar-gelatin biocomposite films; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 115; 2; 8-2013; 604-6121364-5072CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pubmed/23679823info:eu-repo/semantics/altIdentifier/doi/10.1111/jam.12254info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:59:57Zoai:ri.conicet.gov.ar:11336/77809instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:59:57.727CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro antistaphylococcal effects of a novel 45S5 bioglass/agar-gelatin biocomposite films
title In vitro antistaphylococcal effects of a novel 45S5 bioglass/agar-gelatin biocomposite films
spellingShingle In vitro antistaphylococcal effects of a novel 45S5 bioglass/agar-gelatin biocomposite films
Rivadeneira, Josefina
Antibacterial Activity
Bioactive Glass
Biocomposites
Biopolymers
title_short In vitro antistaphylococcal effects of a novel 45S5 bioglass/agar-gelatin biocomposite films
title_full In vitro antistaphylococcal effects of a novel 45S5 bioglass/agar-gelatin biocomposite films
title_fullStr In vitro antistaphylococcal effects of a novel 45S5 bioglass/agar-gelatin biocomposite films
title_full_unstemmed In vitro antistaphylococcal effects of a novel 45S5 bioglass/agar-gelatin biocomposite films
title_sort In vitro antistaphylococcal effects of a novel 45S5 bioglass/agar-gelatin biocomposite films
dc.creator.none.fl_str_mv Rivadeneira, Josefina
Audisio, Marcela Carina
Boccaccini, Aldo R.
Gorustovich Alonso, Alejandro Adrian
author Rivadeneira, Josefina
author_facet Rivadeneira, Josefina
Audisio, Marcela Carina
Boccaccini, Aldo R.
Gorustovich Alonso, Alejandro Adrian
author_role author
author2 Audisio, Marcela Carina
Boccaccini, Aldo R.
Gorustovich Alonso, Alejandro Adrian
author2_role author
author
author
dc.subject.none.fl_str_mv Antibacterial Activity
Bioactive Glass
Biocomposites
Biopolymers
topic Antibacterial Activity
Bioactive Glass
Biocomposites
Biopolymers
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Aims: To assess the antibacterial efficacy of new composite materials developed from microparticles of 45S5 bioactive glass (BG) and agar-gelatin films. Methods and Results: In vitro antibacterial activity was evaluated against Staphylococcus spp. because of the importance of this pathogen in damaged tissues and in failures associated with biomaterial implants. To our knowledge, this is the first paper reporting on the suitable combination of BG and agar-gelatin for bioactive and antibacterial films. Bacterial suspensions up or below 105 CFU ml-1 reflecting situations of wound infection and of noninfection, respectively, were prepared and then put in contact with the biomaterials at 37°C. After 24 and 48 h of incubation, the pH value was measured and the staphylococci strains viability was determined by counting in Mueller-Hinton agar plates. Moreover, the biomaterials were prepared for observation under scanning electron microscopy (SEM). Biocomposites (BCs) showed a strong antibacterial effect against all staphylococci strains tested. Some differences were found depending on the strain, the inoculum size and the contact time. This effect was correlated with an alkalinization of the media. By SEM analyses, no bacterial presence was observed on the surface of BCs in any of the cell concentrations tested at any time. Conclusions: Overall, the coating of 45S5 BG on agar-gelatin films promoted BCs with strong antistaphylococcal activity. The effect was efficient under bacterial concentration up or below 105 CFU ml-1. Additionally, none of the strains were found on BCs surfaces. Significance and Impact of Study: 45S5 bioglass/agar-gelatin biocomposite films are reported for the first time. The results suggest a potential application as wound dressing.
Fil: Rivadeneira, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; Argentina
Fil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Boccaccini, Aldo R.. Universitat Erlangen-Nuremberg; Alemania
Fil: Gorustovich Alonso, Alejandro Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; Argentina
description Aims: To assess the antibacterial efficacy of new composite materials developed from microparticles of 45S5 bioactive glass (BG) and agar-gelatin films. Methods and Results: In vitro antibacterial activity was evaluated against Staphylococcus spp. because of the importance of this pathogen in damaged tissues and in failures associated with biomaterial implants. To our knowledge, this is the first paper reporting on the suitable combination of BG and agar-gelatin for bioactive and antibacterial films. Bacterial suspensions up or below 105 CFU ml-1 reflecting situations of wound infection and of noninfection, respectively, were prepared and then put in contact with the biomaterials at 37°C. After 24 and 48 h of incubation, the pH value was measured and the staphylococci strains viability was determined by counting in Mueller-Hinton agar plates. Moreover, the biomaterials were prepared for observation under scanning electron microscopy (SEM). Biocomposites (BCs) showed a strong antibacterial effect against all staphylococci strains tested. Some differences were found depending on the strain, the inoculum size and the contact time. This effect was correlated with an alkalinization of the media. By SEM analyses, no bacterial presence was observed on the surface of BCs in any of the cell concentrations tested at any time. Conclusions: Overall, the coating of 45S5 BG on agar-gelatin films promoted BCs with strong antistaphylococcal activity. The effect was efficient under bacterial concentration up or below 105 CFU ml-1. Additionally, none of the strains were found on BCs surfaces. Significance and Impact of Study: 45S5 bioglass/agar-gelatin biocomposite films are reported for the first time. The results suggest a potential application as wound dressing.
publishDate 2013
dc.date.none.fl_str_mv 2013-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/77809
Rivadeneira, Josefina; Audisio, Marcela Carina; Boccaccini, Aldo R.; Gorustovich Alonso, Alejandro Adrian; In vitro antistaphylococcal effects of a novel 45S5 bioglass/agar-gelatin biocomposite films; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 115; 2; 8-2013; 604-612
1364-5072
CONICET Digital
CONICET
url http://hdl.handle.net/11336/77809
identifier_str_mv Rivadeneira, Josefina; Audisio, Marcela Carina; Boccaccini, Aldo R.; Gorustovich Alonso, Alejandro Adrian; In vitro antistaphylococcal effects of a novel 45S5 bioglass/agar-gelatin biocomposite films; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 115; 2; 8-2013; 604-612
1364-5072
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pubmed/23679823
info:eu-repo/semantics/altIdentifier/doi/10.1111/jam.12254
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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