Screen-printed enzymatic biosensor modified with carbon nanotube for the methimazole determination in pharmaceuticals formulations

Autores
Martinez, Noelia Anabel; Messina, Germán Alejandro; Bertolino, Franco Adrián; Salinas, Eloy; Raba, Julio
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This paper describes the development of a screen-printed sensor, modified with carbon nanotubes for the rapid and sensitive quantification of methimazole (MT) in pharmaceuticals formulations. Tyrosinase [EC 1.14.18.1], immobilized on a rotating disk, catalyzed the oxidation of catechols to o-benzoquinone, whose back electrochemical reduction was detected on graphite screen-printed electrodes modified with carbon nanotubes at −150 mV. Thus, when MT was added to the solution, this thiol-containing compound participate in Michael type addition reactions with o-benzoquinone to form the corresponding thioquinone derivatives, decreasing the reduction current obtained proportionally to the increase of its concentration. This method could be used to determine MT concentration in the range of 0.074–63.5 μM (r = 0.998). The determination of MT concentration was possible with a detection limit of 0.056 μM in the processing of as many as 25 samples per hour. The biosensor has a reasonable reproducibility (R.S.D. < 3.50%) and a very stable amperometric response toward this compound (more than 1 month). The application of this analysis to different pharmaceutical samples containing MT supports the utility this biosensor.
Fil: Martinez, Noelia Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Bertolino, Franco Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Salinas, Eloy. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Materia
CATECHOL
ENZYMATIC SENSOR
FIA
METHIMAZOLE
SCREEN-PRINTED ELECTRODES
TYROSINASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/159735
Acceder
id CONICETDig_c28098b089f301d15a3e3ce3e58d5ed6
oai_identifier_str oai:ri.conicet.gov.ar:11336/159735
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Screen-printed enzymatic biosensor modified with carbon nanotube for the methimazole determination in pharmaceuticals formulationsMartinez, Noelia AnabelMessina, Germán AlejandroBertolino, Franco AdriánSalinas, EloyRaba, JulioCATECHOLENZYMATIC SENSORFIAMETHIMAZOLESCREEN-PRINTED ELECTRODESTYROSINASEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3This paper describes the development of a screen-printed sensor, modified with carbon nanotubes for the rapid and sensitive quantification of methimazole (MT) in pharmaceuticals formulations. Tyrosinase [EC 1.14.18.1], immobilized on a rotating disk, catalyzed the oxidation of catechols to o-benzoquinone, whose back electrochemical reduction was detected on graphite screen-printed electrodes modified with carbon nanotubes at −150 mV. Thus, when MT was added to the solution, this thiol-containing compound participate in Michael type addition reactions with o-benzoquinone to form the corresponding thioquinone derivatives, decreasing the reduction current obtained proportionally to the increase of its concentration. This method could be used to determine MT concentration in the range of 0.074–63.5 μM (r = 0.998). The determination of MT concentration was possible with a detection limit of 0.056 μM in the processing of as many as 25 samples per hour. The biosensor has a reasonable reproducibility (R.S.D. < 3.50%) and a very stable amperometric response toward this compound (more than 1 month). The application of this analysis to different pharmaceutical samples containing MT supports the utility this biosensor.Fil: Martinez, Noelia Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Bertolino, Franco Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Salinas, Eloy. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaElsevier2008-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/159735Martinez, Noelia Anabel; Messina, Germán Alejandro; Bertolino, Franco Adrián; Salinas, Eloy; Raba, Julio; Screen-printed enzymatic biosensor modified with carbon nanotube for the methimazole determination in pharmaceuticals formulations; Elsevier; Sensors and Actuators B: Chemical; 133; 1; 7-2008; 256-2620925-4005CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0925400508001433info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2008.02.025info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:18:39Zoai:ri.conicet.gov.ar:11336/159735instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:18:39.618CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Screen-printed enzymatic biosensor modified with carbon nanotube for the methimazole determination in pharmaceuticals formulations
title Screen-printed enzymatic biosensor modified with carbon nanotube for the methimazole determination in pharmaceuticals formulations
spellingShingle Screen-printed enzymatic biosensor modified with carbon nanotube for the methimazole determination in pharmaceuticals formulations
Martinez, Noelia Anabel
CATECHOL
ENZYMATIC SENSOR
FIA
METHIMAZOLE
SCREEN-PRINTED ELECTRODES
TYROSINASE
title_short Screen-printed enzymatic biosensor modified with carbon nanotube for the methimazole determination in pharmaceuticals formulations
title_full Screen-printed enzymatic biosensor modified with carbon nanotube for the methimazole determination in pharmaceuticals formulations
title_fullStr Screen-printed enzymatic biosensor modified with carbon nanotube for the methimazole determination in pharmaceuticals formulations
title_full_unstemmed Screen-printed enzymatic biosensor modified with carbon nanotube for the methimazole determination in pharmaceuticals formulations
title_sort Screen-printed enzymatic biosensor modified with carbon nanotube for the methimazole determination in pharmaceuticals formulations
dc.creator.none.fl_str_mv Martinez, Noelia Anabel
Messina, Germán Alejandro
Bertolino, Franco Adrián
Salinas, Eloy
Raba, Julio
author Martinez, Noelia Anabel
author_facet Martinez, Noelia Anabel
Messina, Germán Alejandro
Bertolino, Franco Adrián
Salinas, Eloy
Raba, Julio
author_role author
author2 Messina, Germán Alejandro
Bertolino, Franco Adrián
Salinas, Eloy
Raba, Julio
author2_role author
author
author
author
dc.subject.none.fl_str_mv CATECHOL
ENZYMATIC SENSOR
FIA
METHIMAZOLE
SCREEN-PRINTED ELECTRODES
TYROSINASE
topic CATECHOL
ENZYMATIC SENSOR
FIA
METHIMAZOLE
SCREEN-PRINTED ELECTRODES
TYROSINASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv This paper describes the development of a screen-printed sensor, modified with carbon nanotubes for the rapid and sensitive quantification of methimazole (MT) in pharmaceuticals formulations. Tyrosinase [EC 1.14.18.1], immobilized on a rotating disk, catalyzed the oxidation of catechols to o-benzoquinone, whose back electrochemical reduction was detected on graphite screen-printed electrodes modified with carbon nanotubes at −150 mV. Thus, when MT was added to the solution, this thiol-containing compound participate in Michael type addition reactions with o-benzoquinone to form the corresponding thioquinone derivatives, decreasing the reduction current obtained proportionally to the increase of its concentration. This method could be used to determine MT concentration in the range of 0.074–63.5 μM (r = 0.998). The determination of MT concentration was possible with a detection limit of 0.056 μM in the processing of as many as 25 samples per hour. The biosensor has a reasonable reproducibility (R.S.D. < 3.50%) and a very stable amperometric response toward this compound (more than 1 month). The application of this analysis to different pharmaceutical samples containing MT supports the utility this biosensor.
Fil: Martinez, Noelia Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Bertolino, Franco Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Salinas, Eloy. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
description This paper describes the development of a screen-printed sensor, modified with carbon nanotubes for the rapid and sensitive quantification of methimazole (MT) in pharmaceuticals formulations. Tyrosinase [EC 1.14.18.1], immobilized on a rotating disk, catalyzed the oxidation of catechols to o-benzoquinone, whose back electrochemical reduction was detected on graphite screen-printed electrodes modified with carbon nanotubes at −150 mV. Thus, when MT was added to the solution, this thiol-containing compound participate in Michael type addition reactions with o-benzoquinone to form the corresponding thioquinone derivatives, decreasing the reduction current obtained proportionally to the increase of its concentration. This method could be used to determine MT concentration in the range of 0.074–63.5 μM (r = 0.998). The determination of MT concentration was possible with a detection limit of 0.056 μM in the processing of as many as 25 samples per hour. The biosensor has a reasonable reproducibility (R.S.D. < 3.50%) and a very stable amperometric response toward this compound (more than 1 month). The application of this analysis to different pharmaceutical samples containing MT supports the utility this biosensor.
publishDate 2008
dc.date.none.fl_str_mv 2008-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/159735
Martinez, Noelia Anabel; Messina, Germán Alejandro; Bertolino, Franco Adrián; Salinas, Eloy; Raba, Julio; Screen-printed enzymatic biosensor modified with carbon nanotube for the methimazole determination in pharmaceuticals formulations; Elsevier; Sensors and Actuators B: Chemical; 133; 1; 7-2008; 256-262
0925-4005
CONICET Digital
CONICET
url http://hdl.handle.net/11336/159735
identifier_str_mv Martinez, Noelia Anabel; Messina, Germán Alejandro; Bertolino, Franco Adrián; Salinas, Eloy; Raba, Julio; Screen-printed enzymatic biosensor modified with carbon nanotube for the methimazole determination in pharmaceuticals formulations; Elsevier; Sensors and Actuators B: Chemical; 133; 1; 7-2008; 256-262
0925-4005
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0925400508001433
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2008.02.025
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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