Screen-printed immunosensor for quantification of human serum IgG antibodies to Helicobacter pylori

Autores
Messina, Germán Alejandro; de Vito, Irma Esther; Raba, Julio
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This paper describes the development of a screen-printed immunosensor for the rapid and sensitive quantification of human serum immunoglobulin G (IgG) antibodies to Helicobacter pylori. This microorganism cause peptic ulcers and chronic gastritis, affecting around the 10% of the world population. Antibodies in the serum sample are allowed to react immunologically with the purified H. pylori antigens that are immobilized on graphite screen-printed electrodes (GSPE). The bound antibodies are quantified by alkaline phosphatase (AP) enzyme-labeled second antibodies specific to human IgG. p-Aminophenyl phosphate (p-APP) was converted to p-aminophenol (p-AP) by AP, and an electroactive product was quantified using Osteryoung square wave voltammetry (OSWV). The electrochemical detection can be done within 1 min and total assay time was 25 min. The calculated detection limits for electrochemical detection and the ELISA procedure are 0.5 and 1.8 U ml-1, respectively. Reproducibility assays were made using repetitive standards of H. pylori specific antibody (measured as the activity of the correspondent anti-serum's enzyme conjugated) and the intra- and inter-assay coefficients of variation were below 5%. The electrochemical immunosensor showed higher sensitivity and lower time consumed than the standard spectrophotometric detection ELISA method, demonstrate its potential usefulness for early assessment of human serum immunoglobulin G (IgG) antibodies to H. pylori. © 2007 Elsevier B.V. All rights reserved.
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: de Vito, Irma Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Materia
ALKALINE PHOSPHATASE
FIA
HELICOBACTER PYLORI
IMMUNOSENSOR
P-AMINOPHENYL PHOSPHATE
SCREEN-PRINTED ELECTRODES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/93545
Acceder
id CONICETDig_4fb82b5ffa0ba6acc1537a80b8e56c32
oai_identifier_str oai:ri.conicet.gov.ar:11336/93545
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Screen-printed immunosensor for quantification of human serum IgG antibodies to Helicobacter pyloriMessina, Germán Alejandrode Vito, Irma EstherRaba, JulioALKALINE PHOSPHATASEFIAHELICOBACTER PYLORIIMMUNOSENSORP-AMINOPHENYL PHOSPHATESCREEN-PRINTED ELECTRODEShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1This paper describes the development of a screen-printed immunosensor for the rapid and sensitive quantification of human serum immunoglobulin G (IgG) antibodies to Helicobacter pylori. This microorganism cause peptic ulcers and chronic gastritis, affecting around the 10% of the world population. Antibodies in the serum sample are allowed to react immunologically with the purified H. pylori antigens that are immobilized on graphite screen-printed electrodes (GSPE). The bound antibodies are quantified by alkaline phosphatase (AP) enzyme-labeled second antibodies specific to human IgG. p-Aminophenyl phosphate (p-APP) was converted to p-aminophenol (p-AP) by AP, and an electroactive product was quantified using Osteryoung square wave voltammetry (OSWV). The electrochemical detection can be done within 1 min and total assay time was 25 min. The calculated detection limits for electrochemical detection and the ELISA procedure are 0.5 and 1.8 U ml-1, respectively. Reproducibility assays were made using repetitive standards of H. pylori specific antibody (measured as the activity of the correspondent anti-serum's enzyme conjugated) and the intra- and inter-assay coefficients of variation were below 5%. The electrochemical immunosensor showed higher sensitivity and lower time consumed than the standard spectrophotometric detection ELISA method, demonstrate its potential usefulness for early assessment of human serum immunoglobulin G (IgG) antibodies to H. pylori. © 2007 Elsevier B.V. All rights reserved.Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: de Vito, Irma Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaElsevier Science Sa2007-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/93545Messina, Germán Alejandro; de Vito, Irma Esther; Raba, Julio; Screen-printed immunosensor for quantification of human serum IgG antibodies to Helicobacter pylori; Elsevier Science Sa; Sensors and Actuators B: Chemical; 128; 1; 12-2007; 23-300925-4005CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2007.05.024info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0925400507003681info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:53Zoai:ri.conicet.gov.ar:11336/93545instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:54.306CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Screen-printed immunosensor for quantification of human serum IgG antibodies to Helicobacter pylori
title Screen-printed immunosensor for quantification of human serum IgG antibodies to Helicobacter pylori
spellingShingle Screen-printed immunosensor for quantification of human serum IgG antibodies to Helicobacter pylori
Messina, Germán Alejandro
ALKALINE PHOSPHATASE
FIA
HELICOBACTER PYLORI
IMMUNOSENSOR
P-AMINOPHENYL PHOSPHATE
SCREEN-PRINTED ELECTRODES
title_short Screen-printed immunosensor for quantification of human serum IgG antibodies to Helicobacter pylori
title_full Screen-printed immunosensor for quantification of human serum IgG antibodies to Helicobacter pylori
title_fullStr Screen-printed immunosensor for quantification of human serum IgG antibodies to Helicobacter pylori
title_full_unstemmed Screen-printed immunosensor for quantification of human serum IgG antibodies to Helicobacter pylori
title_sort Screen-printed immunosensor for quantification of human serum IgG antibodies to Helicobacter pylori
dc.creator.none.fl_str_mv Messina, Germán Alejandro
de Vito, Irma Esther
Raba, Julio
author Messina, Germán Alejandro
author_facet Messina, Germán Alejandro
de Vito, Irma Esther
Raba, Julio
author_role author
author2 de Vito, Irma Esther
Raba, Julio
author2_role author
author
dc.subject.none.fl_str_mv ALKALINE PHOSPHATASE
FIA
HELICOBACTER PYLORI
IMMUNOSENSOR
P-AMINOPHENYL PHOSPHATE
SCREEN-PRINTED ELECTRODES
topic ALKALINE PHOSPHATASE
FIA
HELICOBACTER PYLORI
IMMUNOSENSOR
P-AMINOPHENYL PHOSPHATE
SCREEN-PRINTED ELECTRODES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This paper describes the development of a screen-printed immunosensor for the rapid and sensitive quantification of human serum immunoglobulin G (IgG) antibodies to Helicobacter pylori. This microorganism cause peptic ulcers and chronic gastritis, affecting around the 10% of the world population. Antibodies in the serum sample are allowed to react immunologically with the purified H. pylori antigens that are immobilized on graphite screen-printed electrodes (GSPE). The bound antibodies are quantified by alkaline phosphatase (AP) enzyme-labeled second antibodies specific to human IgG. p-Aminophenyl phosphate (p-APP) was converted to p-aminophenol (p-AP) by AP, and an electroactive product was quantified using Osteryoung square wave voltammetry (OSWV). The electrochemical detection can be done within 1 min and total assay time was 25 min. The calculated detection limits for electrochemical detection and the ELISA procedure are 0.5 and 1.8 U ml-1, respectively. Reproducibility assays were made using repetitive standards of H. pylori specific antibody (measured as the activity of the correspondent anti-serum's enzyme conjugated) and the intra- and inter-assay coefficients of variation were below 5%. The electrochemical immunosensor showed higher sensitivity and lower time consumed than the standard spectrophotometric detection ELISA method, demonstrate its potential usefulness for early assessment of human serum immunoglobulin G (IgG) antibodies to H. pylori. © 2007 Elsevier B.V. All rights reserved.
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: de Vito, Irma Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
description This paper describes the development of a screen-printed immunosensor for the rapid and sensitive quantification of human serum immunoglobulin G (IgG) antibodies to Helicobacter pylori. This microorganism cause peptic ulcers and chronic gastritis, affecting around the 10% of the world population. Antibodies in the serum sample are allowed to react immunologically with the purified H. pylori antigens that are immobilized on graphite screen-printed electrodes (GSPE). The bound antibodies are quantified by alkaline phosphatase (AP) enzyme-labeled second antibodies specific to human IgG. p-Aminophenyl phosphate (p-APP) was converted to p-aminophenol (p-AP) by AP, and an electroactive product was quantified using Osteryoung square wave voltammetry (OSWV). The electrochemical detection can be done within 1 min and total assay time was 25 min. The calculated detection limits for electrochemical detection and the ELISA procedure are 0.5 and 1.8 U ml-1, respectively. Reproducibility assays were made using repetitive standards of H. pylori specific antibody (measured as the activity of the correspondent anti-serum's enzyme conjugated) and the intra- and inter-assay coefficients of variation were below 5%. The electrochemical immunosensor showed higher sensitivity and lower time consumed than the standard spectrophotometric detection ELISA method, demonstrate its potential usefulness for early assessment of human serum immunoglobulin G (IgG) antibodies to H. pylori. © 2007 Elsevier B.V. All rights reserved.
publishDate 2007
dc.date.none.fl_str_mv 2007-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/93545
Messina, Germán Alejandro; de Vito, Irma Esther; Raba, Julio; Screen-printed immunosensor for quantification of human serum IgG antibodies to Helicobacter pylori; Elsevier Science Sa; Sensors and Actuators B: Chemical; 128; 1; 12-2007; 23-30
0925-4005
CONICET Digital
CONICET
url http://hdl.handle.net/11336/93545
identifier_str_mv Messina, Germán Alejandro; de Vito, Irma Esther; Raba, Julio; Screen-printed immunosensor for quantification of human serum IgG antibodies to Helicobacter pylori; Elsevier Science Sa; Sensors and Actuators B: Chemical; 128; 1; 12-2007; 23-30
0925-4005
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2007.05.024
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0925400507003681
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Sa
publisher.none.fl_str_mv Elsevier Science Sa
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269124137320448
score 13.13397

Publicaciones similares