Evaluation of the Anti-Inflammatory Effect of Milk Fermented by a Strain of IL-10-Producing Lactococcus lactis Using a Murine Model of Crohn’s Disease

Autores
del Carmen, Silvina Andrea; de Moreno, Maria Alejandra; Perdigon, Gabriela del Valle; Bastos Pereira, Vanessa; Miyoshi, Anderson; Azevedo, Vasco; Leblanc, Jean Guy Joseph
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Interleukin-10 (IL-10) is the most important anti-inflammatory cytokine at intestinal level, and its absence is involved in inflammatory bowel diseases. However, oral treatment with IL-10 is difficult because of its low survival in the gastrointestinal tract and systemic treatments lead to undesirable side effects. The aim of this paper was to evaluate the anti-inflammatory effect of the administration of milks fermented by Lactococcus lactis strains that produce IL-10 under the control of the xylose-inducible expression system using a trinitrobenzenesulfonic acid-induced colitis murine model. Mice that received milks fermented by L. lactis strains producing IL-10 in the cytoplasm (Cyt strain) or  secreted to the product (Sec strain) showed lower damage scores in their large intestines, decreased IFN- gamma levels in their intestinal fluids and lower microbial translocation to liver, compared to mice receiving milk fermented by the wild-type strain or those not receiving any treatment. The results obtained in this study show that the employment of fermented milks as a new form of administration of IL-10-producing L. lactis is effective in the prevention of inflammatory bowel disease in a murine model.
Fil: del Carmen, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Perdigon, Gabriela del Valle. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología. Cátedra de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Bastos Pereira, Vanessa. Universidade Federal de Minas Gerais; Brasil
Fil: Miyoshi, Anderson. Universidade Federal de Minas Gerais; Brasil
Fil: Azevedo, Vasco. Universidade Federal de Minas Gerais; Brasil
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Materia
IL-10
INFLAMMATORY BOWEL DISEASE
LACTIC ACID BACTERIA
GENETIC MODIFIED BACTERIA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/268886
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/268886
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of the Anti-Inflammatory Effect of Milk Fermented by a Strain of IL-10-Producing Lactococcus lactis Using a Murine Model of Crohn’s Diseasedel Carmen, Silvina Andreade Moreno, Maria AlejandraPerdigon, Gabriela del ValleBastos Pereira, VanessaMiyoshi, AndersonAzevedo, VascoLeblanc, Jean Guy JosephIL-10INFLAMMATORY BOWEL DISEASELACTIC ACID BACTERIAGENETIC MODIFIED BACTERIAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Interleukin-10 (IL-10) is the most important anti-inflammatory cytokine at intestinal level, and its absence is involved in inflammatory bowel diseases. However, oral treatment with IL-10 is difficult because of its low survival in the gastrointestinal tract and systemic treatments lead to undesirable side effects. The aim of this paper was to evaluate the anti-inflammatory effect of the administration of milks fermented by Lactococcus lactis strains that produce IL-10 under the control of the xylose-inducible expression system using a trinitrobenzenesulfonic acid-induced colitis murine model. Mice that received milks fermented by L. lactis strains producing IL-10 in the cytoplasm (Cyt strain) or  secreted to the product (Sec strain) showed lower damage scores in their large intestines, decreased IFN- gamma levels in their intestinal fluids and lower microbial translocation to liver, compared to mice receiving milk fermented by the wild-type strain or those not receiving any treatment. The results obtained in this study show that the employment of fermented milks as a new form of administration of IL-10-producing L. lactis is effective in the prevention of inflammatory bowel disease in a murine model.Fil: del Carmen, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Perdigon, Gabriela del Valle. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología. Cátedra de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Bastos Pereira, Vanessa. Universidade Federal de Minas Gerais; BrasilFil: Miyoshi, Anderson. Universidade Federal de Minas Gerais; BrasilFil: Azevedo, Vasco. Universidade Federal de Minas Gerais; BrasilFil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaKarger2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/268886del Carmen, Silvina Andrea; de Moreno, Maria Alejandra; Perdigon, Gabriela del Valle; Bastos Pereira, Vanessa; Miyoshi, Anderson; et al.; Evaluation of the Anti-Inflammatory Effect of Milk Fermented by a Strain of IL-10-Producing Lactococcus lactis Using a Murine Model of Crohn’s Disease; Karger; Journal of Molecular Microbiology and Biotechnology; 21; 3-4; 1-2012; 138-1461464-18012673-1673CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1159/000333830info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:22Zoai:ri.conicet.gov.ar:11336/268886instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:22.801CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of the Anti-Inflammatory Effect of Milk Fermented by a Strain of IL-10-Producing Lactococcus lactis Using a Murine Model of Crohn’s Disease
title Evaluation of the Anti-Inflammatory Effect of Milk Fermented by a Strain of IL-10-Producing Lactococcus lactis Using a Murine Model of Crohn’s Disease
spellingShingle Evaluation of the Anti-Inflammatory Effect of Milk Fermented by a Strain of IL-10-Producing Lactococcus lactis Using a Murine Model of Crohn’s Disease
del Carmen, Silvina Andrea
IL-10
INFLAMMATORY BOWEL DISEASE
LACTIC ACID BACTERIA
GENETIC MODIFIED BACTERIA
title_short Evaluation of the Anti-Inflammatory Effect of Milk Fermented by a Strain of IL-10-Producing Lactococcus lactis Using a Murine Model of Crohn’s Disease
title_full Evaluation of the Anti-Inflammatory Effect of Milk Fermented by a Strain of IL-10-Producing Lactococcus lactis Using a Murine Model of Crohn’s Disease
title_fullStr Evaluation of the Anti-Inflammatory Effect of Milk Fermented by a Strain of IL-10-Producing Lactococcus lactis Using a Murine Model of Crohn’s Disease
title_full_unstemmed Evaluation of the Anti-Inflammatory Effect of Milk Fermented by a Strain of IL-10-Producing Lactococcus lactis Using a Murine Model of Crohn’s Disease
title_sort Evaluation of the Anti-Inflammatory Effect of Milk Fermented by a Strain of IL-10-Producing Lactococcus lactis Using a Murine Model of Crohn’s Disease
dc.creator.none.fl_str_mv del Carmen, Silvina Andrea
de Moreno, Maria Alejandra
Perdigon, Gabriela del Valle
Bastos Pereira, Vanessa
Miyoshi, Anderson
Azevedo, Vasco
Leblanc, Jean Guy Joseph
author del Carmen, Silvina Andrea
author_facet del Carmen, Silvina Andrea
de Moreno, Maria Alejandra
Perdigon, Gabriela del Valle
Bastos Pereira, Vanessa
Miyoshi, Anderson
Azevedo, Vasco
Leblanc, Jean Guy Joseph
author_role author
author2 de Moreno, Maria Alejandra
Perdigon, Gabriela del Valle
Bastos Pereira, Vanessa
Miyoshi, Anderson
Azevedo, Vasco
Leblanc, Jean Guy Joseph
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv IL-10
INFLAMMATORY BOWEL DISEASE
LACTIC ACID BACTERIA
GENETIC MODIFIED BACTERIA
topic IL-10
INFLAMMATORY BOWEL DISEASE
LACTIC ACID BACTERIA
GENETIC MODIFIED BACTERIA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Interleukin-10 (IL-10) is the most important anti-inflammatory cytokine at intestinal level, and its absence is involved in inflammatory bowel diseases. However, oral treatment with IL-10 is difficult because of its low survival in the gastrointestinal tract and systemic treatments lead to undesirable side effects. The aim of this paper was to evaluate the anti-inflammatory effect of the administration of milks fermented by Lactococcus lactis strains that produce IL-10 under the control of the xylose-inducible expression system using a trinitrobenzenesulfonic acid-induced colitis murine model. Mice that received milks fermented by L. lactis strains producing IL-10 in the cytoplasm (Cyt strain) or  secreted to the product (Sec strain) showed lower damage scores in their large intestines, decreased IFN- gamma levels in their intestinal fluids and lower microbial translocation to liver, compared to mice receiving milk fermented by the wild-type strain or those not receiving any treatment. The results obtained in this study show that the employment of fermented milks as a new form of administration of IL-10-producing L. lactis is effective in the prevention of inflammatory bowel disease in a murine model.
Fil: del Carmen, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Perdigon, Gabriela del Valle. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología. Cátedra de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Bastos Pereira, Vanessa. Universidade Federal de Minas Gerais; Brasil
Fil: Miyoshi, Anderson. Universidade Federal de Minas Gerais; Brasil
Fil: Azevedo, Vasco. Universidade Federal de Minas Gerais; Brasil
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
description Interleukin-10 (IL-10) is the most important anti-inflammatory cytokine at intestinal level, and its absence is involved in inflammatory bowel diseases. However, oral treatment with IL-10 is difficult because of its low survival in the gastrointestinal tract and systemic treatments lead to undesirable side effects. The aim of this paper was to evaluate the anti-inflammatory effect of the administration of milks fermented by Lactococcus lactis strains that produce IL-10 under the control of the xylose-inducible expression system using a trinitrobenzenesulfonic acid-induced colitis murine model. Mice that received milks fermented by L. lactis strains producing IL-10 in the cytoplasm (Cyt strain) or  secreted to the product (Sec strain) showed lower damage scores in their large intestines, decreased IFN- gamma levels in their intestinal fluids and lower microbial translocation to liver, compared to mice receiving milk fermented by the wild-type strain or those not receiving any treatment. The results obtained in this study show that the employment of fermented milks as a new form of administration of IL-10-producing L. lactis is effective in the prevention of inflammatory bowel disease in a murine model.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/268886
del Carmen, Silvina Andrea; de Moreno, Maria Alejandra; Perdigon, Gabriela del Valle; Bastos Pereira, Vanessa; Miyoshi, Anderson; et al.; Evaluation of the Anti-Inflammatory Effect of Milk Fermented by a Strain of IL-10-Producing Lactococcus lactis Using a Murine Model of Crohn’s Disease; Karger; Journal of Molecular Microbiology and Biotechnology; 21; 3-4; 1-2012; 138-146
1464-1801
2673-1673
CONICET Digital
CONICET
url http://hdl.handle.net/11336/268886
identifier_str_mv del Carmen, Silvina Andrea; de Moreno, Maria Alejandra; Perdigon, Gabriela del Valle; Bastos Pereira, Vanessa; Miyoshi, Anderson; et al.; Evaluation of the Anti-Inflammatory Effect of Milk Fermented by a Strain of IL-10-Producing Lactococcus lactis Using a Murine Model of Crohn’s Disease; Karger; Journal of Molecular Microbiology and Biotechnology; 21; 3-4; 1-2012; 138-146
1464-1801
2673-1673
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1159/000333830
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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