Photodynamic toxicity and its prevention by antioxidative agents in Bufo arenarum embryos

Autores
Stockert, Juan C.; Herkovits, Jorge
Año de publicación
2003
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work we describe an experimental model to evaluate the photodynamic toxicity on amphibian embryos, as well as the protective effect of antioxidants against the lethal oxidative stress induced by photosensitization. Bufo arenarum embryos were treated with 10 mg/l methylene blue (MB) in AMPHITOX solution for 72 h and then irradiated with a red laser or white light for variable times. Both light sources affected the survival of MB-treated animals and lethal effects occurred within the initial 12 h post-irradiation. For white light irradiation, the most effective phototoxic condition in our study, the LD10, 50 and 90 at 6 h post-irradiation corresponded to 13.57, 19.87 and 29.10 J/cm2, respectively. To explore the action of antioxidants against the photogenerated oxidative stress, MB-treated embryos were incubated with 1 mM glutathione (GSH) or ascorbic acid (AA) during 48 h before irradiation. For GSH and 21.6 J/cm2 irradiation, the survival increased from 20 to 90%, whereas 100% survival was achieved with AA even after 43.2 J/cm2 irradiation. These results indicate that both the lethal photodynamic effect and its prevention by antioxidants can be evaluated by means of a simple toxicity test employing amphibian embryos.
Fil: Stockert, Juan C.. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina
Fil: Herkovits, Jorge. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Amphibian embryo
Oxidative stress
Protection
Phototoxicity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/243329
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/243329
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Photodynamic toxicity and its prevention by antioxidative agents in Bufo arenarum embryosStockert, Juan C.Herkovits, JorgeAmphibian embryoOxidative stressProtectionPhototoxicityhttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1In this work we describe an experimental model to evaluate the photodynamic toxicity on amphibian embryos, as well as the protective effect of antioxidants against the lethal oxidative stress induced by photosensitization. Bufo arenarum embryos were treated with 10 mg/l methylene blue (MB) in AMPHITOX solution for 72 h and then irradiated with a red laser or white light for variable times. Both light sources affected the survival of MB-treated animals and lethal effects occurred within the initial 12 h post-irradiation. For white light irradiation, the most effective phototoxic condition in our study, the LD10, 50 and 90 at 6 h post-irradiation corresponded to 13.57, 19.87 and 29.10 J/cm2, respectively. To explore the action of antioxidants against the photogenerated oxidative stress, MB-treated embryos were incubated with 1 mM glutathione (GSH) or ascorbic acid (AA) during 48 h before irradiation. For GSH and 21.6 J/cm2 irradiation, the survival increased from 20 to 90%, whereas 100% survival was achieved with AA even after 43.2 J/cm2 irradiation. These results indicate that both the lethal photodynamic effect and its prevention by antioxidants can be evaluated by means of a simple toxicity test employing amphibian embryos.Fil: Stockert, Juan C.. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; ArgentinaFil: Herkovits, Jorge. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Ireland2003-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/243329Stockert, Juan C.; Herkovits, Jorge; Photodynamic toxicity and its prevention by antioxidative agents in Bufo arenarum embryos; Elsevier Ireland; Toxicology; 192; 2-3; 11-2003; 211-2180300-483XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0300483X03003342info:eu-repo/semantics/altIdentifier/doi/10.1016/S0300-483X(03)00334-2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:43:07Zoai:ri.conicet.gov.ar:11336/243329instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:43:07.847CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Photodynamic toxicity and its prevention by antioxidative agents in Bufo arenarum embryos
title Photodynamic toxicity and its prevention by antioxidative agents in Bufo arenarum embryos
spellingShingle Photodynamic toxicity and its prevention by antioxidative agents in Bufo arenarum embryos
Stockert, Juan C.
Amphibian embryo
Oxidative stress
Protection
Phototoxicity
title_short Photodynamic toxicity and its prevention by antioxidative agents in Bufo arenarum embryos
title_full Photodynamic toxicity and its prevention by antioxidative agents in Bufo arenarum embryos
title_fullStr Photodynamic toxicity and its prevention by antioxidative agents in Bufo arenarum embryos
title_full_unstemmed Photodynamic toxicity and its prevention by antioxidative agents in Bufo arenarum embryos
title_sort Photodynamic toxicity and its prevention by antioxidative agents in Bufo arenarum embryos
dc.creator.none.fl_str_mv Stockert, Juan C.
Herkovits, Jorge
author Stockert, Juan C.
author_facet Stockert, Juan C.
Herkovits, Jorge
author_role author
author2 Herkovits, Jorge
author2_role author
dc.subject.none.fl_str_mv Amphibian embryo
Oxidative stress
Protection
Phototoxicity
topic Amphibian embryo
Oxidative stress
Protection
Phototoxicity
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this work we describe an experimental model to evaluate the photodynamic toxicity on amphibian embryos, as well as the protective effect of antioxidants against the lethal oxidative stress induced by photosensitization. Bufo arenarum embryos were treated with 10 mg/l methylene blue (MB) in AMPHITOX solution for 72 h and then irradiated with a red laser or white light for variable times. Both light sources affected the survival of MB-treated animals and lethal effects occurred within the initial 12 h post-irradiation. For white light irradiation, the most effective phototoxic condition in our study, the LD10, 50 and 90 at 6 h post-irradiation corresponded to 13.57, 19.87 and 29.10 J/cm2, respectively. To explore the action of antioxidants against the photogenerated oxidative stress, MB-treated embryos were incubated with 1 mM glutathione (GSH) or ascorbic acid (AA) during 48 h before irradiation. For GSH and 21.6 J/cm2 irradiation, the survival increased from 20 to 90%, whereas 100% survival was achieved with AA even after 43.2 J/cm2 irradiation. These results indicate that both the lethal photodynamic effect and its prevention by antioxidants can be evaluated by means of a simple toxicity test employing amphibian embryos.
Fil: Stockert, Juan C.. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina
Fil: Herkovits, Jorge. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description In this work we describe an experimental model to evaluate the photodynamic toxicity on amphibian embryos, as well as the protective effect of antioxidants against the lethal oxidative stress induced by photosensitization. Bufo arenarum embryos were treated with 10 mg/l methylene blue (MB) in AMPHITOX solution for 72 h and then irradiated with a red laser or white light for variable times. Both light sources affected the survival of MB-treated animals and lethal effects occurred within the initial 12 h post-irradiation. For white light irradiation, the most effective phototoxic condition in our study, the LD10, 50 and 90 at 6 h post-irradiation corresponded to 13.57, 19.87 and 29.10 J/cm2, respectively. To explore the action of antioxidants against the photogenerated oxidative stress, MB-treated embryos were incubated with 1 mM glutathione (GSH) or ascorbic acid (AA) during 48 h before irradiation. For GSH and 21.6 J/cm2 irradiation, the survival increased from 20 to 90%, whereas 100% survival was achieved with AA even after 43.2 J/cm2 irradiation. These results indicate that both the lethal photodynamic effect and its prevention by antioxidants can be evaluated by means of a simple toxicity test employing amphibian embryos.
publishDate 2003
dc.date.none.fl_str_mv 2003-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/243329
Stockert, Juan C.; Herkovits, Jorge; Photodynamic toxicity and its prevention by antioxidative agents in Bufo arenarum embryos; Elsevier Ireland; Toxicology; 192; 2-3; 11-2003; 211-218
0300-483X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/243329
identifier_str_mv Stockert, Juan C.; Herkovits, Jorge; Photodynamic toxicity and its prevention by antioxidative agents in Bufo arenarum embryos; Elsevier Ireland; Toxicology; 192; 2-3; 11-2003; 211-218
0300-483X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0300483X03003342
info:eu-repo/semantics/altIdentifier/doi/10.1016/S0300-483X(03)00334-2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614465617657856
score 13.070432

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