Evaluation of stress responses to water molds in embryos of Physalaemus albonotatus (Anura: Leptodactylidae)
- Autores
- Ghirardi, Romina; Cazenave, Jimena; Lopez, Javier Alejandro; Antoniazzi, Carolina Elisabet; Perotti, Maria Gabriela
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Infections by water molds of the genus Saprolegnia (Oomycetes) have been associated with the death ofeggs and embryos in many anuran species; however, how water molds induce physiological responses in early andsusceptible developmental stages of anurans is poorly known. To assess whether or not embryos of Physalaemusalbonotatus (immobile and susceptible life stages) respond to Oomycetes infection, we performed a 4-d experimentexposing early stage embryos to the water mold Saprolegnia-like sp. We assessed mortality, hatching time, andoxidative stress (antioxidant enzyme activities and lipid peroxidation levels). Presence of water molds in infectedembryos was not evident to the naked eye until 1 to 1.5 d after inoculation. Mortality was significantly higher in thewater mold treatment than in the control treatment. Embryos exposed to Saprolegnia-like sp. hatched 24 h earlierthan those in control treatment. Among enzymatic activities, only catalase enzyme showed a significant depletionin embryos exposed to water molds compared to control groups. These results emphasize the need to exploreoxidative stress markers along with embryonic development at key stages, focusing on those early stages wherehatching is induced by water molds infection.
Fil: Ghirardi, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Cazenave, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Lopez, Javier Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Antoniazzi, Carolina Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Perotti, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina - Materia
-
amphibian embryo
hatching time
mortality
oxidative stress - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/88562
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CONICET Digital (CONICET) |
spelling |
Evaluation of stress responses to water molds in embryos of Physalaemus albonotatus (Anura: Leptodactylidae)Ghirardi, RominaCazenave, JimenaLopez, Javier AlejandroAntoniazzi, Carolina ElisabetPerotti, Maria Gabrielaamphibian embryohatching timemortalityoxidative stresshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Infections by water molds of the genus Saprolegnia (Oomycetes) have been associated with the death ofeggs and embryos in many anuran species; however, how water molds induce physiological responses in early andsusceptible developmental stages of anurans is poorly known. To assess whether or not embryos of Physalaemusalbonotatus (immobile and susceptible life stages) respond to Oomycetes infection, we performed a 4-d experimentexposing early stage embryos to the water mold Saprolegnia-like sp. We assessed mortality, hatching time, andoxidative stress (antioxidant enzyme activities and lipid peroxidation levels). Presence of water molds in infectedembryos was not evident to the naked eye until 1 to 1.5 d after inoculation. Mortality was significantly higher in thewater mold treatment than in the control treatment. Embryos exposed to Saprolegnia-like sp. hatched 24 h earlierthan those in control treatment. Among enzymatic activities, only catalase enzyme showed a significant depletionin embryos exposed to water molds compared to control groups. These results emphasize the need to exploreoxidative stress markers along with embryonic development at key stages, focusing on those early stages wherehatching is induced by water molds infection.Fil: Ghirardi, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; ArgentinaFil: Cazenave, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; ArgentinaFil: Lopez, Javier Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; ArgentinaFil: Antoniazzi, Carolina Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; ArgentinaFil: Perotti, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; ArgentinaHerpetological Conservation and Biology2018-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/88562Ghirardi, Romina; Cazenave, Jimena; Lopez, Javier Alejandro; Antoniazzi, Carolina Elisabet; Perotti, Maria Gabriela; Evaluation of stress responses to water molds in embryos of Physalaemus albonotatus (Anura: Leptodactylidae); Herpetological Conservation and Biology; Herpetological Conservation and Biology; 13; 7-2018; 213-2231931-7603CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://herpconbio.org/volumes.htmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:26Zoai:ri.conicet.gov.ar:11336/88562instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:27.061CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Evaluation of stress responses to water molds in embryos of Physalaemus albonotatus (Anura: Leptodactylidae) |
title |
Evaluation of stress responses to water molds in embryos of Physalaemus albonotatus (Anura: Leptodactylidae) |
spellingShingle |
Evaluation of stress responses to water molds in embryos of Physalaemus albonotatus (Anura: Leptodactylidae) Ghirardi, Romina amphibian embryo hatching time mortality oxidative stress |
title_short |
Evaluation of stress responses to water molds in embryos of Physalaemus albonotatus (Anura: Leptodactylidae) |
title_full |
Evaluation of stress responses to water molds in embryos of Physalaemus albonotatus (Anura: Leptodactylidae) |
title_fullStr |
Evaluation of stress responses to water molds in embryos of Physalaemus albonotatus (Anura: Leptodactylidae) |
title_full_unstemmed |
Evaluation of stress responses to water molds in embryos of Physalaemus albonotatus (Anura: Leptodactylidae) |
title_sort |
Evaluation of stress responses to water molds in embryos of Physalaemus albonotatus (Anura: Leptodactylidae) |
dc.creator.none.fl_str_mv |
Ghirardi, Romina Cazenave, Jimena Lopez, Javier Alejandro Antoniazzi, Carolina Elisabet Perotti, Maria Gabriela |
author |
Ghirardi, Romina |
author_facet |
Ghirardi, Romina Cazenave, Jimena Lopez, Javier Alejandro Antoniazzi, Carolina Elisabet Perotti, Maria Gabriela |
author_role |
author |
author2 |
Cazenave, Jimena Lopez, Javier Alejandro Antoniazzi, Carolina Elisabet Perotti, Maria Gabriela |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
amphibian embryo hatching time mortality oxidative stress |
topic |
amphibian embryo hatching time mortality oxidative stress |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Infections by water molds of the genus Saprolegnia (Oomycetes) have been associated with the death ofeggs and embryos in many anuran species; however, how water molds induce physiological responses in early andsusceptible developmental stages of anurans is poorly known. To assess whether or not embryos of Physalaemusalbonotatus (immobile and susceptible life stages) respond to Oomycetes infection, we performed a 4-d experimentexposing early stage embryos to the water mold Saprolegnia-like sp. We assessed mortality, hatching time, andoxidative stress (antioxidant enzyme activities and lipid peroxidation levels). Presence of water molds in infectedembryos was not evident to the naked eye until 1 to 1.5 d after inoculation. Mortality was significantly higher in thewater mold treatment than in the control treatment. Embryos exposed to Saprolegnia-like sp. hatched 24 h earlierthan those in control treatment. Among enzymatic activities, only catalase enzyme showed a significant depletionin embryos exposed to water molds compared to control groups. These results emphasize the need to exploreoxidative stress markers along with embryonic development at key stages, focusing on those early stages wherehatching is induced by water molds infection. Fil: Ghirardi, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina Fil: Cazenave, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina Fil: Lopez, Javier Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina Fil: Antoniazzi, Carolina Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina Fil: Perotti, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina |
description |
Infections by water molds of the genus Saprolegnia (Oomycetes) have been associated with the death ofeggs and embryos in many anuran species; however, how water molds induce physiological responses in early andsusceptible developmental stages of anurans is poorly known. To assess whether or not embryos of Physalaemusalbonotatus (immobile and susceptible life stages) respond to Oomycetes infection, we performed a 4-d experimentexposing early stage embryos to the water mold Saprolegnia-like sp. We assessed mortality, hatching time, andoxidative stress (antioxidant enzyme activities and lipid peroxidation levels). Presence of water molds in infectedembryos was not evident to the naked eye until 1 to 1.5 d after inoculation. Mortality was significantly higher in thewater mold treatment than in the control treatment. Embryos exposed to Saprolegnia-like sp. hatched 24 h earlierthan those in control treatment. Among enzymatic activities, only catalase enzyme showed a significant depletionin embryos exposed to water molds compared to control groups. These results emphasize the need to exploreoxidative stress markers along with embryonic development at key stages, focusing on those early stages wherehatching is induced by water molds infection. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/88562 Ghirardi, Romina; Cazenave, Jimena; Lopez, Javier Alejandro; Antoniazzi, Carolina Elisabet; Perotti, Maria Gabriela; Evaluation of stress responses to water molds in embryos of Physalaemus albonotatus (Anura: Leptodactylidae); Herpetological Conservation and Biology; Herpetological Conservation and Biology; 13; 7-2018; 213-223 1931-7603 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/88562 |
identifier_str_mv |
Ghirardi, Romina; Cazenave, Jimena; Lopez, Javier Alejandro; Antoniazzi, Carolina Elisabet; Perotti, Maria Gabriela; Evaluation of stress responses to water molds in embryos of Physalaemus albonotatus (Anura: Leptodactylidae); Herpetological Conservation and Biology; Herpetological Conservation and Biology; 13; 7-2018; 213-223 1931-7603 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://herpconbio.org/volumes.html |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Herpetological Conservation and Biology |
publisher.none.fl_str_mv |
Herpetological Conservation and Biology |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613808272703488 |
score |
13.070432 |