Evaluation of stress responses to water molds in embryos of Physalaemus albonotatus (Anura: Leptodactylidae)

Autores
Ghirardi, Romina; Cazenave, Jimena; Lopez, Javier Alejandro; Antoniazzi, Carolina Elisabet; Perotti, Maria Gabriela
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Infections by water molds of the genus Saprolegnia (Oomycetes) have been associated with the death ofeggs and embryos in many anuran species; however, how water molds induce physiological responses in early andsusceptible developmental stages of anurans is poorly known. To assess whether or not embryos of Physalaemusalbonotatus (immobile and susceptible life stages) respond to Oomycetes infection, we performed a 4-d experimentexposing early stage embryos to the water mold Saprolegnia-like sp. We assessed mortality, hatching time, andoxidative stress (antioxidant enzyme activities and lipid peroxidation levels). Presence of water molds in infectedembryos was not evident to the naked eye until 1 to 1.5 d after inoculation. Mortality was significantly higher in thewater mold treatment than in the control treatment. Embryos exposed to Saprolegnia-like sp. hatched 24 h earlierthan those in control treatment. Among enzymatic activities, only catalase enzyme showed a significant depletionin embryos exposed to water molds compared to control groups. These results emphasize the need to exploreoxidative stress markers along with embryonic development at key stages, focusing on those early stages wherehatching is induced by water molds infection.
Fil: Ghirardi, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Cazenave, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Lopez, Javier Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Antoniazzi, Carolina Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Perotti, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina
Materia
amphibian embryo
hatching time
mortality
oxidative stress
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/88562
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/88562
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of stress responses to water molds in embryos of Physalaemus albonotatus (Anura: Leptodactylidae)Ghirardi, RominaCazenave, JimenaLopez, Javier AlejandroAntoniazzi, Carolina ElisabetPerotti, Maria Gabrielaamphibian embryohatching timemortalityoxidative stresshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Infections by water molds of the genus Saprolegnia (Oomycetes) have been associated with the death ofeggs and embryos in many anuran species; however, how water molds induce physiological responses in early andsusceptible developmental stages of anurans is poorly known. To assess whether or not embryos of Physalaemusalbonotatus (immobile and susceptible life stages) respond to Oomycetes infection, we performed a 4-d experimentexposing early stage embryos to the water mold Saprolegnia-like sp. We assessed mortality, hatching time, andoxidative stress (antioxidant enzyme activities and lipid peroxidation levels). Presence of water molds in infectedembryos was not evident to the naked eye until 1 to 1.5 d after inoculation. Mortality was significantly higher in thewater mold treatment than in the control treatment. Embryos exposed to Saprolegnia-like sp. hatched 24 h earlierthan those in control treatment. Among enzymatic activities, only catalase enzyme showed a significant depletionin embryos exposed to water molds compared to control groups. These results emphasize the need to exploreoxidative stress markers along with embryonic development at key stages, focusing on those early stages wherehatching is induced by water molds infection.Fil: Ghirardi, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; ArgentinaFil: Cazenave, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; ArgentinaFil: Lopez, Javier Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; ArgentinaFil: Antoniazzi, Carolina Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; ArgentinaFil: Perotti, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; ArgentinaHerpetological Conservation and Biology2018-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/88562Ghirardi, Romina; Cazenave, Jimena; Lopez, Javier Alejandro; Antoniazzi, Carolina Elisabet; Perotti, Maria Gabriela; Evaluation of stress responses to water molds in embryos of Physalaemus albonotatus (Anura: Leptodactylidae); Herpetological Conservation and Biology; Herpetological Conservation and Biology; 13; 7-2018; 213-2231931-7603CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://herpconbio.org/volumes.htmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:57:29Zoai:ri.conicet.gov.ar:11336/88562instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:57:29.4CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of stress responses to water molds in embryos of Physalaemus albonotatus (Anura: Leptodactylidae)
title Evaluation of stress responses to water molds in embryos of Physalaemus albonotatus (Anura: Leptodactylidae)
spellingShingle Evaluation of stress responses to water molds in embryos of Physalaemus albonotatus (Anura: Leptodactylidae)
Ghirardi, Romina
amphibian embryo
hatching time
mortality
oxidative stress
title_short Evaluation of stress responses to water molds in embryos of Physalaemus albonotatus (Anura: Leptodactylidae)
title_full Evaluation of stress responses to water molds in embryos of Physalaemus albonotatus (Anura: Leptodactylidae)
title_fullStr Evaluation of stress responses to water molds in embryos of Physalaemus albonotatus (Anura: Leptodactylidae)
title_full_unstemmed Evaluation of stress responses to water molds in embryos of Physalaemus albonotatus (Anura: Leptodactylidae)
title_sort Evaluation of stress responses to water molds in embryos of Physalaemus albonotatus (Anura: Leptodactylidae)
dc.creator.none.fl_str_mv Ghirardi, Romina
Cazenave, Jimena
Lopez, Javier Alejandro
Antoniazzi, Carolina Elisabet
Perotti, Maria Gabriela
author Ghirardi, Romina
author_facet Ghirardi, Romina
Cazenave, Jimena
Lopez, Javier Alejandro
Antoniazzi, Carolina Elisabet
Perotti, Maria Gabriela
author_role author
author2 Cazenave, Jimena
Lopez, Javier Alejandro
Antoniazzi, Carolina Elisabet
Perotti, Maria Gabriela
author2_role author
author
author
author
dc.subject.none.fl_str_mv amphibian embryo
hatching time
mortality
oxidative stress
topic amphibian embryo
hatching time
mortality
oxidative stress
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Infections by water molds of the genus Saprolegnia (Oomycetes) have been associated with the death ofeggs and embryos in many anuran species; however, how water molds induce physiological responses in early andsusceptible developmental stages of anurans is poorly known. To assess whether or not embryos of Physalaemusalbonotatus (immobile and susceptible life stages) respond to Oomycetes infection, we performed a 4-d experimentexposing early stage embryos to the water mold Saprolegnia-like sp. We assessed mortality, hatching time, andoxidative stress (antioxidant enzyme activities and lipid peroxidation levels). Presence of water molds in infectedembryos was not evident to the naked eye until 1 to 1.5 d after inoculation. Mortality was significantly higher in thewater mold treatment than in the control treatment. Embryos exposed to Saprolegnia-like sp. hatched 24 h earlierthan those in control treatment. Among enzymatic activities, only catalase enzyme showed a significant depletionin embryos exposed to water molds compared to control groups. These results emphasize the need to exploreoxidative stress markers along with embryonic development at key stages, focusing on those early stages wherehatching is induced by water molds infection.
Fil: Ghirardi, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Cazenave, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Lopez, Javier Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Antoniazzi, Carolina Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Perotti, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina
description Infections by water molds of the genus Saprolegnia (Oomycetes) have been associated with the death ofeggs and embryos in many anuran species; however, how water molds induce physiological responses in early andsusceptible developmental stages of anurans is poorly known. To assess whether or not embryos of Physalaemusalbonotatus (immobile and susceptible life stages) respond to Oomycetes infection, we performed a 4-d experimentexposing early stage embryos to the water mold Saprolegnia-like sp. We assessed mortality, hatching time, andoxidative stress (antioxidant enzyme activities and lipid peroxidation levels). Presence of water molds in infectedembryos was not evident to the naked eye until 1 to 1.5 d after inoculation. Mortality was significantly higher in thewater mold treatment than in the control treatment. Embryos exposed to Saprolegnia-like sp. hatched 24 h earlierthan those in control treatment. Among enzymatic activities, only catalase enzyme showed a significant depletionin embryos exposed to water molds compared to control groups. These results emphasize the need to exploreoxidative stress markers along with embryonic development at key stages, focusing on those early stages wherehatching is induced by water molds infection.
publishDate 2018
dc.date.none.fl_str_mv 2018-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/88562
Ghirardi, Romina; Cazenave, Jimena; Lopez, Javier Alejandro; Antoniazzi, Carolina Elisabet; Perotti, Maria Gabriela; Evaluation of stress responses to water molds in embryos of Physalaemus albonotatus (Anura: Leptodactylidae); Herpetological Conservation and Biology; Herpetological Conservation and Biology; 13; 7-2018; 213-223
1931-7603
CONICET Digital
CONICET
url http://hdl.handle.net/11336/88562
identifier_str_mv Ghirardi, Romina; Cazenave, Jimena; Lopez, Javier Alejandro; Antoniazzi, Carolina Elisabet; Perotti, Maria Gabriela; Evaluation of stress responses to water molds in embryos of Physalaemus albonotatus (Anura: Leptodactylidae); Herpetological Conservation and Biology; Herpetological Conservation and Biology; 13; 7-2018; 213-223
1931-7603
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://herpconbio.org/volumes.html
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Herpetological Conservation and Biology
publisher.none.fl_str_mv Herpetological Conservation and Biology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083113940156416
score 13.22299

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