Estradiol uptake, toxicity, metabolism, and adverse effects on cadmium-treated amphibian embryos

Autores
Fridman, Osvaldo; Corró, Lucrecia; Herkovits, Jorge
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The exposure of Bufo arenarum embryos to 25 µmol/L 17β-estradiol (E2) resulted in 100% lethality within 48 hr, whereas 10 µmol//L E2 was the no observed effect concentration value for shortterm chronic (7 days) exposure. The toxicity profile curves show that lethal effects were proportional to the E2 concentration and the time of exposure. The E2 uptake resulted in 20.1 ng E2/mg embryo at 8 hr posttreatment, but 67.3% of this value was achieved during the first 30 min of incubation with this estrogen. Regarding metabolism, the embryos synthesize estrone (E1) from E2 by means of 17β-hydroxysteroid dehydrogenase. Simultaneous treatments of Bufo arenarum embryos with 1 mg/L Cd2+ and 0.1, 1, or 10 µmol/L E2 enhanced the lethality exerted by cadmium in 76.7, 80, and 83.3% of embryos, respectively. The results indicate that estrogenic endocrine disruptors could have an adverse effect on amphibian embryos and enhance the toxic effect of Cd on amphibian embryos. This study points to the possibility of using the AMPHITOX test as a screening method for potential endocrine disruption as well as the combined effects of chemical mixtures.
Fil: Fridman, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Pro Salud y Medio Ambiente; Argentina
Fil: Corró, Lucrecia. Fundación Pro Salud y Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Herkovits, Jorge. Fundación Pro Salud y Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
estradiol toxicity
cadmium
endocrine disruption
amphibian embryo
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/243330
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/243330
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Estradiol uptake, toxicity, metabolism, and adverse effects on cadmium-treated amphibian embryosFridman, OsvaldoCorró, LucreciaHerkovits, Jorgeestradiol toxicitycadmiumendocrine disruptionamphibian embryohttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The exposure of Bufo arenarum embryos to 25 µmol/L 17β-estradiol (E2) resulted in 100% lethality within 48 hr, whereas 10 µmol//L E2 was the no observed effect concentration value for shortterm chronic (7 days) exposure. The toxicity profile curves show that lethal effects were proportional to the E2 concentration and the time of exposure. The E2 uptake resulted in 20.1 ng E2/mg embryo at 8 hr posttreatment, but 67.3% of this value was achieved during the first 30 min of incubation with this estrogen. Regarding metabolism, the embryos synthesize estrone (E1) from E2 by means of 17β-hydroxysteroid dehydrogenase. Simultaneous treatments of Bufo arenarum embryos with 1 mg/L Cd2+ and 0.1, 1, or 10 µmol/L E2 enhanced the lethality exerted by cadmium in 76.7, 80, and 83.3% of embryos, respectively. The results indicate that estrogenic endocrine disruptors could have an adverse effect on amphibian embryos and enhance the toxic effect of Cd on amphibian embryos. This study points to the possibility of using the AMPHITOX test as a screening method for potential endocrine disruption as well as the combined effects of chemical mixtures.Fil: Fridman, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Pro Salud y Medio Ambiente; ArgentinaFil: Corró, Lucrecia. Fundación Pro Salud y Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Herkovits, Jorge. Fundación Pro Salud y Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaU.S. Department of Health and Human Sciences. Public Health and Science2004-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/243330Fridman, Osvaldo; Corró, Lucrecia; Herkovits, Jorge; Estradiol uptake, toxicity, metabolism, and adverse effects on cadmium-treated amphibian embryos; U.S. Department of Health and Human Sciences. Public Health and Science; Environmental Health Perspectives; 112; 8; 6-2004; 862-8660091-6765CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://ehp.niehs.nih.gov/doi/10.1289/ehp.6647?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmedinfo:eu-repo/semantics/altIdentifier/doi/10.1289/ehp.6647info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:26:59Zoai:ri.conicet.gov.ar:11336/243330instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:26:59.442CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Estradiol uptake, toxicity, metabolism, and adverse effects on cadmium-treated amphibian embryos
title Estradiol uptake, toxicity, metabolism, and adverse effects on cadmium-treated amphibian embryos
spellingShingle Estradiol uptake, toxicity, metabolism, and adverse effects on cadmium-treated amphibian embryos
Fridman, Osvaldo
estradiol toxicity
cadmium
endocrine disruption
amphibian embryo
title_short Estradiol uptake, toxicity, metabolism, and adverse effects on cadmium-treated amphibian embryos
title_full Estradiol uptake, toxicity, metabolism, and adverse effects on cadmium-treated amphibian embryos
title_fullStr Estradiol uptake, toxicity, metabolism, and adverse effects on cadmium-treated amphibian embryos
title_full_unstemmed Estradiol uptake, toxicity, metabolism, and adverse effects on cadmium-treated amphibian embryos
title_sort Estradiol uptake, toxicity, metabolism, and adverse effects on cadmium-treated amphibian embryos
dc.creator.none.fl_str_mv Fridman, Osvaldo
Corró, Lucrecia
Herkovits, Jorge
author Fridman, Osvaldo
author_facet Fridman, Osvaldo
Corró, Lucrecia
Herkovits, Jorge
author_role author
author2 Corró, Lucrecia
Herkovits, Jorge
author2_role author
author
dc.subject.none.fl_str_mv estradiol toxicity
cadmium
endocrine disruption
amphibian embryo
topic estradiol toxicity
cadmium
endocrine disruption
amphibian embryo
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The exposure of Bufo arenarum embryos to 25 µmol/L 17β-estradiol (E2) resulted in 100% lethality within 48 hr, whereas 10 µmol//L E2 was the no observed effect concentration value for shortterm chronic (7 days) exposure. The toxicity profile curves show that lethal effects were proportional to the E2 concentration and the time of exposure. The E2 uptake resulted in 20.1 ng E2/mg embryo at 8 hr posttreatment, but 67.3% of this value was achieved during the first 30 min of incubation with this estrogen. Regarding metabolism, the embryos synthesize estrone (E1) from E2 by means of 17β-hydroxysteroid dehydrogenase. Simultaneous treatments of Bufo arenarum embryos with 1 mg/L Cd2+ and 0.1, 1, or 10 µmol/L E2 enhanced the lethality exerted by cadmium in 76.7, 80, and 83.3% of embryos, respectively. The results indicate that estrogenic endocrine disruptors could have an adverse effect on amphibian embryos and enhance the toxic effect of Cd on amphibian embryos. This study points to the possibility of using the AMPHITOX test as a screening method for potential endocrine disruption as well as the combined effects of chemical mixtures.
Fil: Fridman, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Pro Salud y Medio Ambiente; Argentina
Fil: Corró, Lucrecia. Fundación Pro Salud y Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Herkovits, Jorge. Fundación Pro Salud y Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The exposure of Bufo arenarum embryos to 25 µmol/L 17β-estradiol (E2) resulted in 100% lethality within 48 hr, whereas 10 µmol//L E2 was the no observed effect concentration value for shortterm chronic (7 days) exposure. The toxicity profile curves show that lethal effects were proportional to the E2 concentration and the time of exposure. The E2 uptake resulted in 20.1 ng E2/mg embryo at 8 hr posttreatment, but 67.3% of this value was achieved during the first 30 min of incubation with this estrogen. Regarding metabolism, the embryos synthesize estrone (E1) from E2 by means of 17β-hydroxysteroid dehydrogenase. Simultaneous treatments of Bufo arenarum embryos with 1 mg/L Cd2+ and 0.1, 1, or 10 µmol/L E2 enhanced the lethality exerted by cadmium in 76.7, 80, and 83.3% of embryos, respectively. The results indicate that estrogenic endocrine disruptors could have an adverse effect on amphibian embryos and enhance the toxic effect of Cd on amphibian embryos. This study points to the possibility of using the AMPHITOX test as a screening method for potential endocrine disruption as well as the combined effects of chemical mixtures.
publishDate 2004
dc.date.none.fl_str_mv 2004-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/243330
Fridman, Osvaldo; Corró, Lucrecia; Herkovits, Jorge; Estradiol uptake, toxicity, metabolism, and adverse effects on cadmium-treated amphibian embryos; U.S. Department of Health and Human Sciences. Public Health and Science; Environmental Health Perspectives; 112; 8; 6-2004; 862-866
0091-6765
CONICET Digital
CONICET
url http://hdl.handle.net/11336/243330
identifier_str_mv Fridman, Osvaldo; Corró, Lucrecia; Herkovits, Jorge; Estradiol uptake, toxicity, metabolism, and adverse effects on cadmium-treated amphibian embryos; U.S. Department of Health and Human Sciences. Public Health and Science; Environmental Health Perspectives; 112; 8; 6-2004; 862-866
0091-6765
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ehp.niehs.nih.gov/doi/10.1289/ehp.6647?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmed
info:eu-repo/semantics/altIdentifier/doi/10.1289/ehp.6647
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv U.S. Department of Health and Human Sciences. Public Health and Science
publisher.none.fl_str_mv U.S. Department of Health and Human Sciences. Public Health and Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614271472762880
score 13.070432

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