Tissular Distribution of Argentinean Strains of Bovine Herpesvirus Type 4 (BoHV-4) in Experimentally-Infected Calves

Autores
Morán, Pedro Edgardo; Perez, Sandra; Garcia, Jo; Castro, Alejandra Nelly Cristina; Spetter Lucas, Maximiliano Joaquín; Odeón, Anselmo Carlos; Verna, Andrea Elizabeth
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Although bovine herpesvirus type 4 (BoHV-4) is primarily associated with reproductive disorders of cattle, it canproduce a variety of clinical signs. To determine the distribution, the presence and type of microscopic lesions causedby BoHV-4 strains of different genotypes an in vivo model, calves were infected with three phylogenetically differentArgentinean BoHV-4 strains. Samples from nasal and ocular secretions, peripheral blood leukocytes, tissues andserum were analyzed. BoHV-4 was isolated from nasal and ocular secretions at 7 and 14 days post-inoculation (dpi).Viral DNA was detected by nested PCR in peripheral blood leukocytes at 14 and 21 dpi for two out of three strainsand in tissues, such as nervous system, trachea, pulmonary and retropharyngeal lymph nodes, spleen and kidney, at21 dpi. Antibody levels detected by viral seroneutralization test were mostly low and varied widely for the differentstrains. The tissue distribution of the BoHV-4 strains and the variations observed in the levels of neutralizing antibodiesindicate that certain differences can be established among the patterns of biological behavior of each strain.This is an initial step to get insight into the biological characteristics of Argentinean BoHV-4 isolates. However,further evaluation involving a higher number of inoculated animals will be required to be conclusive on this aspect.
Fil: Morán, Pedro Edgardo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virologia; Argentina
Fil: Perez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virologia; Argentina
Fil: Garcia, Jo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virologia; Argentina
Fil: Castro, Alejandra Nelly Cristina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virologia; Argentina
Fil: Spetter Lucas, Maximiliano Joaquín. Ministerio de Ciencia, Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica. Fondo para la Investigación Científica y Tecnológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Odeón, Anselmo Carlos. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virologia; Argentina
Fil: Verna, Andrea Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
Materia
BoHV-4
Argentinean strains
Tissue Distribution
Cattle
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/63392
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Tissular Distribution of Argentinean Strains of Bovine Herpesvirus Type 4 (BoHV-4) in Experimentally-Infected CalvesMorán, Pedro EdgardoPerez, SandraGarcia, JoCastro, Alejandra Nelly CristinaSpetter Lucas, Maximiliano JoaquínOdeón, Anselmo CarlosVerna, Andrea ElizabethBoHV-4Argentinean strainsTissue DistributionCattlehttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Although bovine herpesvirus type 4 (BoHV-4) is primarily associated with reproductive disorders of cattle, it canproduce a variety of clinical signs. To determine the distribution, the presence and type of microscopic lesions causedby BoHV-4 strains of different genotypes an in vivo model, calves were infected with three phylogenetically differentArgentinean BoHV-4 strains. Samples from nasal and ocular secretions, peripheral blood leukocytes, tissues andserum were analyzed. BoHV-4 was isolated from nasal and ocular secretions at 7 and 14 days post-inoculation (dpi).Viral DNA was detected by nested PCR in peripheral blood leukocytes at 14 and 21 dpi for two out of three strainsand in tissues, such as nervous system, trachea, pulmonary and retropharyngeal lymph nodes, spleen and kidney, at21 dpi. Antibody levels detected by viral seroneutralization test were mostly low and varied widely for the differentstrains. The tissue distribution of the BoHV-4 strains and the variations observed in the levels of neutralizing antibodiesindicate that certain differences can be established among the patterns of biological behavior of each strain.This is an initial step to get insight into the biological characteristics of Argentinean BoHV-4 isolates. However,further evaluation involving a higher number of inoculated animals will be required to be conclusive on this aspect.Fil: Morán, Pedro Edgardo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virologia; ArgentinaFil: Perez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virologia; ArgentinaFil: Garcia, Jo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virologia; ArgentinaFil: Castro, Alejandra Nelly Cristina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virologia; ArgentinaFil: Spetter Lucas, Maximiliano Joaquín. Ministerio de Ciencia, Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica. Fondo para la Investigación Científica y Tecnológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Odeón, Anselmo Carlos. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virologia; ArgentinaFil: Verna, Andrea Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; ArgentinaSociedade Brasileira de Virologia2017-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/63392Morán, Pedro Edgardo; Perez, Sandra; Garcia, Jo; Castro, Alejandra Nelly Cristina; Spetter Lucas, Maximiliano Joaquín; et al.; Tissular Distribution of Argentinean Strains of Bovine Herpesvirus Type 4 (BoHV-4) in Experimentally-Infected Calves; Sociedade Brasileira de Virologia; Virus Reviews And Research; 22; 9-2017; 12-191519-25632357-9323CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.17525/vrrjournal.v22i1.330info:eu-repo/semantics/altIdentifier/url/http://157.86.113.86/index.php/vrrjournal/article/view/330info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:23:11Zoai:ri.conicet.gov.ar:11336/63392instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:23:11.668CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Tissular Distribution of Argentinean Strains of Bovine Herpesvirus Type 4 (BoHV-4) in Experimentally-Infected Calves
title Tissular Distribution of Argentinean Strains of Bovine Herpesvirus Type 4 (BoHV-4) in Experimentally-Infected Calves
spellingShingle Tissular Distribution of Argentinean Strains of Bovine Herpesvirus Type 4 (BoHV-4) in Experimentally-Infected Calves
Morán, Pedro Edgardo
BoHV-4
Argentinean strains
Tissue Distribution
Cattle
title_short Tissular Distribution of Argentinean Strains of Bovine Herpesvirus Type 4 (BoHV-4) in Experimentally-Infected Calves
title_full Tissular Distribution of Argentinean Strains of Bovine Herpesvirus Type 4 (BoHV-4) in Experimentally-Infected Calves
title_fullStr Tissular Distribution of Argentinean Strains of Bovine Herpesvirus Type 4 (BoHV-4) in Experimentally-Infected Calves
title_full_unstemmed Tissular Distribution of Argentinean Strains of Bovine Herpesvirus Type 4 (BoHV-4) in Experimentally-Infected Calves
title_sort Tissular Distribution of Argentinean Strains of Bovine Herpesvirus Type 4 (BoHV-4) in Experimentally-Infected Calves
dc.creator.none.fl_str_mv Morán, Pedro Edgardo
Perez, Sandra
Garcia, Jo
Castro, Alejandra Nelly Cristina
Spetter Lucas, Maximiliano Joaquín
Odeón, Anselmo Carlos
Verna, Andrea Elizabeth
author Morán, Pedro Edgardo
author_facet Morán, Pedro Edgardo
Perez, Sandra
Garcia, Jo
Castro, Alejandra Nelly Cristina
Spetter Lucas, Maximiliano Joaquín
Odeón, Anselmo Carlos
Verna, Andrea Elizabeth
author_role author
author2 Perez, Sandra
Garcia, Jo
Castro, Alejandra Nelly Cristina
Spetter Lucas, Maximiliano Joaquín
Odeón, Anselmo Carlos
Verna, Andrea Elizabeth
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BoHV-4
Argentinean strains
Tissue Distribution
Cattle
topic BoHV-4
Argentinean strains
Tissue Distribution
Cattle
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Although bovine herpesvirus type 4 (BoHV-4) is primarily associated with reproductive disorders of cattle, it canproduce a variety of clinical signs. To determine the distribution, the presence and type of microscopic lesions causedby BoHV-4 strains of different genotypes an in vivo model, calves were infected with three phylogenetically differentArgentinean BoHV-4 strains. Samples from nasal and ocular secretions, peripheral blood leukocytes, tissues andserum were analyzed. BoHV-4 was isolated from nasal and ocular secretions at 7 and 14 days post-inoculation (dpi).Viral DNA was detected by nested PCR in peripheral blood leukocytes at 14 and 21 dpi for two out of three strainsand in tissues, such as nervous system, trachea, pulmonary and retropharyngeal lymph nodes, spleen and kidney, at21 dpi. Antibody levels detected by viral seroneutralization test were mostly low and varied widely for the differentstrains. The tissue distribution of the BoHV-4 strains and the variations observed in the levels of neutralizing antibodiesindicate that certain differences can be established among the patterns of biological behavior of each strain.This is an initial step to get insight into the biological characteristics of Argentinean BoHV-4 isolates. However,further evaluation involving a higher number of inoculated animals will be required to be conclusive on this aspect.
Fil: Morán, Pedro Edgardo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virologia; Argentina
Fil: Perez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virologia; Argentina
Fil: Garcia, Jo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virologia; Argentina
Fil: Castro, Alejandra Nelly Cristina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virologia; Argentina
Fil: Spetter Lucas, Maximiliano Joaquín. Ministerio de Ciencia, Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica. Fondo para la Investigación Científica y Tecnológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Odeón, Anselmo Carlos. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virologia; Argentina
Fil: Verna, Andrea Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
description Although bovine herpesvirus type 4 (BoHV-4) is primarily associated with reproductive disorders of cattle, it canproduce a variety of clinical signs. To determine the distribution, the presence and type of microscopic lesions causedby BoHV-4 strains of different genotypes an in vivo model, calves were infected with three phylogenetically differentArgentinean BoHV-4 strains. Samples from nasal and ocular secretions, peripheral blood leukocytes, tissues andserum were analyzed. BoHV-4 was isolated from nasal and ocular secretions at 7 and 14 days post-inoculation (dpi).Viral DNA was detected by nested PCR in peripheral blood leukocytes at 14 and 21 dpi for two out of three strainsand in tissues, such as nervous system, trachea, pulmonary and retropharyngeal lymph nodes, spleen and kidney, at21 dpi. Antibody levels detected by viral seroneutralization test were mostly low and varied widely for the differentstrains. The tissue distribution of the BoHV-4 strains and the variations observed in the levels of neutralizing antibodiesindicate that certain differences can be established among the patterns of biological behavior of each strain.This is an initial step to get insight into the biological characteristics of Argentinean BoHV-4 isolates. However,further evaluation involving a higher number of inoculated animals will be required to be conclusive on this aspect.
publishDate 2017
dc.date.none.fl_str_mv 2017-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/63392
Morán, Pedro Edgardo; Perez, Sandra; Garcia, Jo; Castro, Alejandra Nelly Cristina; Spetter Lucas, Maximiliano Joaquín; et al.; Tissular Distribution of Argentinean Strains of Bovine Herpesvirus Type 4 (BoHV-4) in Experimentally-Infected Calves; Sociedade Brasileira de Virologia; Virus Reviews And Research; 22; 9-2017; 12-19
1519-2563
2357-9323
CONICET Digital
CONICET
url http://hdl.handle.net/11336/63392
identifier_str_mv Morán, Pedro Edgardo; Perez, Sandra; Garcia, Jo; Castro, Alejandra Nelly Cristina; Spetter Lucas, Maximiliano Joaquín; et al.; Tissular Distribution of Argentinean Strains of Bovine Herpesvirus Type 4 (BoHV-4) in Experimentally-Infected Calves; Sociedade Brasileira de Virologia; Virus Reviews And Research; 22; 9-2017; 12-19
1519-2563
2357-9323
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.17525/vrrjournal.v22i1.330
info:eu-repo/semantics/altIdentifier/url/http://157.86.113.86/index.php/vrrjournal/article/view/330
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Virologia
publisher.none.fl_str_mv Sociedade Brasileira de Virologia
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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