Tissular distribution of argentinean strains of bovine herpesvirus type 4 (BoHV-4) in experimentally-infected calves
- Autores
- Morán, Pedro Edgardo; Pérez, Sandra Elizabet; García, Jorge Pablo; Castro, Alejandra Nelly Cristina; Sppeter, Lucas Maximiliano; Odeon, Anselmo Carlos; Verna, Andrea Elizabeth
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Although bovine herpesvirus type 4 (BoHV-4) is primarily associated with reproductive disorders of cattle, it can produce a variety of clinical signs. To determine the distribution, the presence and type of microscopic lesions caused by BoHV-4 strains of different genotypes an in vivo model, calves were infected with three phylogenetically different Argentinean BoHV-4 strains. Samples from nasal and ocular secretions, peripheral blood leukocytes, tissues and serum were analyzed. BoHV-4 was isolated from nasal and ocular secretions at 7 and 14 days post-inoculation (dpi). Viral DNA was detected by nested PCR in peripheral blood leukocytes at 14 and 21 dpi for two out of three strains and in tissues, such as nervous system, trachea, pulmonary and retropharyngeal lymph nodes, spleen and kidney, at 21 dpi. Antibody levels detected by viral seroneutralization test were mostly low and varied widely for the different strains. The tissue distribution of the BoHV-4 strains and the variations observed in the levels of neutralizing antibodies indicate that certain differences can be established among the patterns of biological behavior of each strain. This is an initial step to get insight into the biological characteristics of Argentinean BoHV-4 isolates. However, further evaluation involving a higher number of inoculated animals will be required to be conclusive on this aspect.
EEA Balcarce
Fil: Morán, Pedro Edgardo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Perez, Sandra Elizabet. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: García, Jorge Pablo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Castro, Alejandra Nelly Cristina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Sppeter, Lucas Maximiliano. Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT); Argentina
Fil: Odeon, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Fuente
- Virus Reviews & Research 22 : 12-19 (2017)
- Materia
-
Ternero
Virus de los Animales
Herpes Virus Bovino
Distribución en Tejidos
Ganado Bovino
Animal Viruses
Bovine Herpesvirus
Tissue Distribution
Cattle
Argentina - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/2023
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Tissular distribution of argentinean strains of bovine herpesvirus type 4 (BoHV-4) in experimentally-infected calvesMorán, Pedro EdgardoPérez, Sandra ElizabetGarcía, Jorge PabloCastro, Alejandra Nelly CristinaSppeter, Lucas MaximilianoOdeon, Anselmo CarlosVerna, Andrea ElizabethTerneroVirus de los AnimalesHerpes Virus BovinoDistribución en TejidosGanado BovinoAnimal VirusesBovine HerpesvirusTissue DistributionCattleArgentinaAlthough bovine herpesvirus type 4 (BoHV-4) is primarily associated with reproductive disorders of cattle, it can produce a variety of clinical signs. To determine the distribution, the presence and type of microscopic lesions caused by BoHV-4 strains of different genotypes an in vivo model, calves were infected with three phylogenetically different Argentinean BoHV-4 strains. Samples from nasal and ocular secretions, peripheral blood leukocytes, tissues and serum were analyzed. BoHV-4 was isolated from nasal and ocular secretions at 7 and 14 days post-inoculation (dpi). Viral DNA was detected by nested PCR in peripheral blood leukocytes at 14 and 21 dpi for two out of three strains and in tissues, such as nervous system, trachea, pulmonary and retropharyngeal lymph nodes, spleen and kidney, at 21 dpi. Antibody levels detected by viral seroneutralization test were mostly low and varied widely for the different strains. The tissue distribution of the BoHV-4 strains and the variations observed in the levels of neutralizing antibodies indicate that certain differences can be established among the patterns of biological behavior of each strain. This is an initial step to get insight into the biological characteristics of Argentinean BoHV-4 isolates. However, further evaluation involving a higher number of inoculated animals will be required to be conclusive on this aspect.EEA BalcarceFil: Morán, Pedro Edgardo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Perez, Sandra Elizabet. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: García, Jorge Pablo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Castro, Alejandra Nelly Cristina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Sppeter, Lucas Maximiliano. Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT); ArgentinaFil: Odeon, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina2018-03-15T11:26:57Z2018-03-15T11:26:57Z2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/2023http://157.86.113.86/index.php/vrrjournal/article/view/330/3031519-2563 (Print)2357-9323 (Online)http://dx.doi.org/10.17525/vrrjournal.v22i1.330Virus Reviews & Research 22 : 12-19 (2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:47:10Zoai:localhost:20.500.12123/2023instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:10.893INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Tissular distribution of argentinean strains of bovine herpesvirus type 4 (BoHV-4) in experimentally-infected calves |
title |
Tissular distribution of argentinean strains of bovine herpesvirus type 4 (BoHV-4) in experimentally-infected calves |
spellingShingle |
Tissular distribution of argentinean strains of bovine herpesvirus type 4 (BoHV-4) in experimentally-infected calves Morán, Pedro Edgardo Ternero Virus de los Animales Herpes Virus Bovino Distribución en Tejidos Ganado Bovino Animal Viruses Bovine Herpesvirus Tissue Distribution Cattle Argentina |
title_short |
Tissular distribution of argentinean strains of bovine herpesvirus type 4 (BoHV-4) in experimentally-infected calves |
title_full |
Tissular distribution of argentinean strains of bovine herpesvirus type 4 (BoHV-4) in experimentally-infected calves |
title_fullStr |
Tissular distribution of argentinean strains of bovine herpesvirus type 4 (BoHV-4) in experimentally-infected calves |
title_full_unstemmed |
Tissular distribution of argentinean strains of bovine herpesvirus type 4 (BoHV-4) in experimentally-infected calves |
title_sort |
Tissular distribution of argentinean strains of bovine herpesvirus type 4 (BoHV-4) in experimentally-infected calves |
dc.creator.none.fl_str_mv |
Morán, Pedro Edgardo Pérez, Sandra Elizabet García, Jorge Pablo Castro, Alejandra Nelly Cristina Sppeter, Lucas Maximiliano Odeon, Anselmo Carlos Verna, Andrea Elizabeth |
author |
Morán, Pedro Edgardo |
author_facet |
Morán, Pedro Edgardo Pérez, Sandra Elizabet García, Jorge Pablo Castro, Alejandra Nelly Cristina Sppeter, Lucas Maximiliano Odeon, Anselmo Carlos Verna, Andrea Elizabeth |
author_role |
author |
author2 |
Pérez, Sandra Elizabet García, Jorge Pablo Castro, Alejandra Nelly Cristina Sppeter, Lucas Maximiliano Odeon, Anselmo Carlos Verna, Andrea Elizabeth |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Ternero Virus de los Animales Herpes Virus Bovino Distribución en Tejidos Ganado Bovino Animal Viruses Bovine Herpesvirus Tissue Distribution Cattle Argentina |
topic |
Ternero Virus de los Animales Herpes Virus Bovino Distribución en Tejidos Ganado Bovino Animal Viruses Bovine Herpesvirus Tissue Distribution Cattle Argentina |
dc.description.none.fl_txt_mv |
Although bovine herpesvirus type 4 (BoHV-4) is primarily associated with reproductive disorders of cattle, it can produce a variety of clinical signs. To determine the distribution, the presence and type of microscopic lesions caused by BoHV-4 strains of different genotypes an in vivo model, calves were infected with three phylogenetically different Argentinean BoHV-4 strains. Samples from nasal and ocular secretions, peripheral blood leukocytes, tissues and serum were analyzed. BoHV-4 was isolated from nasal and ocular secretions at 7 and 14 days post-inoculation (dpi). Viral DNA was detected by nested PCR in peripheral blood leukocytes at 14 and 21 dpi for two out of three strains and in tissues, such as nervous system, trachea, pulmonary and retropharyngeal lymph nodes, spleen and kidney, at 21 dpi. Antibody levels detected by viral seroneutralization test were mostly low and varied widely for the different strains. The tissue distribution of the BoHV-4 strains and the variations observed in the levels of neutralizing antibodies indicate that certain differences can be established among the patterns of biological behavior of each strain. This is an initial step to get insight into the biological characteristics of Argentinean BoHV-4 isolates. However, further evaluation involving a higher number of inoculated animals will be required to be conclusive on this aspect. EEA Balcarce Fil: Morán, Pedro Edgardo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina Fil: Perez, Sandra Elizabet. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigación Veterinaria de Tandil; Argentina Fil: García, Jorge Pablo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina Fil: Castro, Alejandra Nelly Cristina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina Fil: Sppeter, Lucas Maximiliano. Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT); Argentina Fil: Odeon, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
Although bovine herpesvirus type 4 (BoHV-4) is primarily associated with reproductive disorders of cattle, it can produce a variety of clinical signs. To determine the distribution, the presence and type of microscopic lesions caused by BoHV-4 strains of different genotypes an in vivo model, calves were infected with three phylogenetically different Argentinean BoHV-4 strains. Samples from nasal and ocular secretions, peripheral blood leukocytes, tissues and serum were analyzed. BoHV-4 was isolated from nasal and ocular secretions at 7 and 14 days post-inoculation (dpi). Viral DNA was detected by nested PCR in peripheral blood leukocytes at 14 and 21 dpi for two out of three strains and in tissues, such as nervous system, trachea, pulmonary and retropharyngeal lymph nodes, spleen and kidney, at 21 dpi. Antibody levels detected by viral seroneutralization test were mostly low and varied widely for the different strains. The tissue distribution of the BoHV-4 strains and the variations observed in the levels of neutralizing antibodies indicate that certain differences can be established among the patterns of biological behavior of each strain. This is an initial step to get insight into the biological characteristics of Argentinean BoHV-4 isolates. However, further evaluation involving a higher number of inoculated animals will be required to be conclusive on this aspect. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 2018-03-15T11:26:57Z 2018-03-15T11:26:57Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/2023 http://157.86.113.86/index.php/vrrjournal/article/view/330/303 1519-2563 (Print) 2357-9323 (Online) http://dx.doi.org/10.17525/vrrjournal.v22i1.330 |
url |
http://hdl.handle.net/20.500.12123/2023 http://157.86.113.86/index.php/vrrjournal/article/view/330/303 http://dx.doi.org/10.17525/vrrjournal.v22i1.330 |
identifier_str_mv |
1519-2563 (Print) 2357-9323 (Online) |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Virus Reviews & Research 22 : 12-19 (2017) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
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Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
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tripaldi.nicolas@inta.gob.ar |
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