Tissular distribution of argentinean strains of bovine herpesvirus type 4 (BoHV-4) in experimentally-infected calves

Autores
Morán, Pedro Edgardo; Pérez, Sandra Elizabet; García, Jorge Pablo; Castro, Alejandra Nelly Cristina; Sppeter, Lucas Maximiliano; Odeon, Anselmo Carlos; Verna, Andrea Elizabeth
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Although bovine herpesvirus type 4 (BoHV-4) is primarily associated with reproductive disorders of cattle, it can produce a variety of clinical signs. To determine the distribution, the presence and type of microscopic lesions caused by BoHV-4 strains of different genotypes an in vivo model, calves were infected with three phylogenetically different Argentinean BoHV-4 strains. Samples from nasal and ocular secretions, peripheral blood leukocytes, tissues and serum were analyzed. BoHV-4 was isolated from nasal and ocular secretions at 7 and 14 days post-inoculation (dpi). Viral DNA was detected by nested PCR in peripheral blood leukocytes at 14 and 21 dpi for two out of three strains and in tissues, such as nervous system, trachea, pulmonary and retropharyngeal lymph nodes, spleen and kidney, at 21 dpi. Antibody levels detected by viral seroneutralization test were mostly low and varied widely for the different strains. The tissue distribution of the BoHV-4 strains and the variations observed in the levels of neutralizing antibodies indicate that certain differences can be established among the patterns of biological behavior of each strain. This is an initial step to get insight into the biological characteristics of Argentinean BoHV-4 isolates. However, further evaluation involving a higher number of inoculated animals will be required to be conclusive on this aspect.
EEA Balcarce
Fil: Morán, Pedro Edgardo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Perez, Sandra Elizabet. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: García, Jorge Pablo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Castro, Alejandra Nelly Cristina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Sppeter, Lucas Maximiliano. Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT); Argentina
Fil: Odeon, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Virus Reviews & Research 22 : 12-19 (2017)
Materia
Ternero
Virus de los Animales
Herpes Virus Bovino
Distribución en Tejidos
Ganado Bovino
Animal Viruses
Bovine Herpesvirus
Tissue Distribution
Cattle
Argentina
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2023
Acceder
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oai_identifier_str oai:localhost:20.500.12123/2023
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Tissular distribution of argentinean strains of bovine herpesvirus type 4 (BoHV-4) in experimentally-infected calvesMorán, Pedro EdgardoPérez, Sandra ElizabetGarcía, Jorge PabloCastro, Alejandra Nelly CristinaSppeter, Lucas MaximilianoOdeon, Anselmo CarlosVerna, Andrea ElizabethTerneroVirus de los AnimalesHerpes Virus BovinoDistribución en TejidosGanado BovinoAnimal VirusesBovine HerpesvirusTissue DistributionCattleArgentinaAlthough bovine herpesvirus type 4 (BoHV-4) is primarily associated with reproductive disorders of cattle, it can produce a variety of clinical signs. To determine the distribution, the presence and type of microscopic lesions caused by BoHV-4 strains of different genotypes an in vivo model, calves were infected with three phylogenetically different Argentinean BoHV-4 strains. Samples from nasal and ocular secretions, peripheral blood leukocytes, tissues and serum were analyzed. BoHV-4 was isolated from nasal and ocular secretions at 7 and 14 days post-inoculation (dpi). Viral DNA was detected by nested PCR in peripheral blood leukocytes at 14 and 21 dpi for two out of three strains and in tissues, such as nervous system, trachea, pulmonary and retropharyngeal lymph nodes, spleen and kidney, at 21 dpi. Antibody levels detected by viral seroneutralization test were mostly low and varied widely for the different strains. The tissue distribution of the BoHV-4 strains and the variations observed in the levels of neutralizing antibodies indicate that certain differences can be established among the patterns of biological behavior of each strain. This is an initial step to get insight into the biological characteristics of Argentinean BoHV-4 isolates. However, further evaluation involving a higher number of inoculated animals will be required to be conclusive on this aspect.EEA BalcarceFil: Morán, Pedro Edgardo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Perez, Sandra Elizabet. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: García, Jorge Pablo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Castro, Alejandra Nelly Cristina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Sppeter, Lucas Maximiliano. Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT); ArgentinaFil: Odeon, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina2018-03-15T11:26:57Z2018-03-15T11:26:57Z2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/2023http://157.86.113.86/index.php/vrrjournal/article/view/330/3031519-2563 (Print)2357-9323 (Online)http://dx.doi.org/10.17525/vrrjournal.v22i1.330Virus Reviews & Research 22 : 12-19 (2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:16Zoai:localhost:20.500.12123/2023instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:16.501INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Tissular distribution of argentinean strains of bovine herpesvirus type 4 (BoHV-4) in experimentally-infected calves
title Tissular distribution of argentinean strains of bovine herpesvirus type 4 (BoHV-4) in experimentally-infected calves
spellingShingle Tissular distribution of argentinean strains of bovine herpesvirus type 4 (BoHV-4) in experimentally-infected calves
Morán, Pedro Edgardo
Ternero
Virus de los Animales
Herpes Virus Bovino
Distribución en Tejidos
Ganado Bovino
Animal Viruses
Bovine Herpesvirus
Tissue Distribution
Cattle
Argentina
title_short Tissular distribution of argentinean strains of bovine herpesvirus type 4 (BoHV-4) in experimentally-infected calves
title_full Tissular distribution of argentinean strains of bovine herpesvirus type 4 (BoHV-4) in experimentally-infected calves
title_fullStr Tissular distribution of argentinean strains of bovine herpesvirus type 4 (BoHV-4) in experimentally-infected calves
title_full_unstemmed Tissular distribution of argentinean strains of bovine herpesvirus type 4 (BoHV-4) in experimentally-infected calves
title_sort Tissular distribution of argentinean strains of bovine herpesvirus type 4 (BoHV-4) in experimentally-infected calves
dc.creator.none.fl_str_mv Morán, Pedro Edgardo
Pérez, Sandra Elizabet
García, Jorge Pablo
Castro, Alejandra Nelly Cristina
Sppeter, Lucas Maximiliano
Odeon, Anselmo Carlos
Verna, Andrea Elizabeth
author Morán, Pedro Edgardo
author_facet Morán, Pedro Edgardo
Pérez, Sandra Elizabet
García, Jorge Pablo
Castro, Alejandra Nelly Cristina
Sppeter, Lucas Maximiliano
Odeon, Anselmo Carlos
Verna, Andrea Elizabeth
author_role author
author2 Pérez, Sandra Elizabet
García, Jorge Pablo
Castro, Alejandra Nelly Cristina
Sppeter, Lucas Maximiliano
Odeon, Anselmo Carlos
Verna, Andrea Elizabeth
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ternero
Virus de los Animales
Herpes Virus Bovino
Distribución en Tejidos
Ganado Bovino
Animal Viruses
Bovine Herpesvirus
Tissue Distribution
Cattle
Argentina
topic Ternero
Virus de los Animales
Herpes Virus Bovino
Distribución en Tejidos
Ganado Bovino
Animal Viruses
Bovine Herpesvirus
Tissue Distribution
Cattle
Argentina
dc.description.none.fl_txt_mv Although bovine herpesvirus type 4 (BoHV-4) is primarily associated with reproductive disorders of cattle, it can produce a variety of clinical signs. To determine the distribution, the presence and type of microscopic lesions caused by BoHV-4 strains of different genotypes an in vivo model, calves were infected with three phylogenetically different Argentinean BoHV-4 strains. Samples from nasal and ocular secretions, peripheral blood leukocytes, tissues and serum were analyzed. BoHV-4 was isolated from nasal and ocular secretions at 7 and 14 days post-inoculation (dpi). Viral DNA was detected by nested PCR in peripheral blood leukocytes at 14 and 21 dpi for two out of three strains and in tissues, such as nervous system, trachea, pulmonary and retropharyngeal lymph nodes, spleen and kidney, at 21 dpi. Antibody levels detected by viral seroneutralization test were mostly low and varied widely for the different strains. The tissue distribution of the BoHV-4 strains and the variations observed in the levels of neutralizing antibodies indicate that certain differences can be established among the patterns of biological behavior of each strain. This is an initial step to get insight into the biological characteristics of Argentinean BoHV-4 isolates. However, further evaluation involving a higher number of inoculated animals will be required to be conclusive on this aspect.
EEA Balcarce
Fil: Morán, Pedro Edgardo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Perez, Sandra Elizabet. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: García, Jorge Pablo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Castro, Alejandra Nelly Cristina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Sppeter, Lucas Maximiliano. Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT); Argentina
Fil: Odeon, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Although bovine herpesvirus type 4 (BoHV-4) is primarily associated with reproductive disorders of cattle, it can produce a variety of clinical signs. To determine the distribution, the presence and type of microscopic lesions caused by BoHV-4 strains of different genotypes an in vivo model, calves were infected with three phylogenetically different Argentinean BoHV-4 strains. Samples from nasal and ocular secretions, peripheral blood leukocytes, tissues and serum were analyzed. BoHV-4 was isolated from nasal and ocular secretions at 7 and 14 days post-inoculation (dpi). Viral DNA was detected by nested PCR in peripheral blood leukocytes at 14 and 21 dpi for two out of three strains and in tissues, such as nervous system, trachea, pulmonary and retropharyngeal lymph nodes, spleen and kidney, at 21 dpi. Antibody levels detected by viral seroneutralization test were mostly low and varied widely for the different strains. The tissue distribution of the BoHV-4 strains and the variations observed in the levels of neutralizing antibodies indicate that certain differences can be established among the patterns of biological behavior of each strain. This is an initial step to get insight into the biological characteristics of Argentinean BoHV-4 isolates. However, further evaluation involving a higher number of inoculated animals will be required to be conclusive on this aspect.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018-03-15T11:26:57Z
2018-03-15T11:26:57Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/2023
http://157.86.113.86/index.php/vrrjournal/article/view/330/303
1519-2563 (Print)
2357-9323 (Online)
http://dx.doi.org/10.17525/vrrjournal.v22i1.330
url http://hdl.handle.net/20.500.12123/2023
http://157.86.113.86/index.php/vrrjournal/article/view/330/303
http://dx.doi.org/10.17525/vrrjournal.v22i1.330
identifier_str_mv 1519-2563 (Print)
2357-9323 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Virus Reviews & Research 22 : 12-19 (2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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