Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs

Autores
Cardoso, Nancy Patricia; Rivero, Carla Ivanna; Castillo, Mariángeles; Mansilla, Florencia Celeste; Pastorino, Florencia Laura; Piccirilli, Guadalupe; Alonso, Laura; Martínez, Gustavo; Di Lullo, David; Bentancor, Leticia Veronica; Capozzo, Alejandra Victoria
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The Coronavirus Disease 2019 (COVID-19) is a zoonotic disease caused by the pandemic virus SARS-CoV-2. Domestic and wild animals are susceptible to infection and are potential reservoirs for virus variants. To date, there is no information about the exposure of companion animals in Buenos Aires Suburbs, the area with the largest population in Argentina where the highest number of COVID-19 human cases occurred during the first infection wave. Here we developed a multi-species indirect ELISA to measure antibodies reactive to the SARS-CoV-2 receptor-binding domain (RBD) from several vertebrates constituting the class Mammalia, making it a valuable tool for field serosurveillance. The ELISA cut-off value was estimated by sera from dogs, cats, cattle, and pigs sampled before 2019 (n = 170), considering a 98% percentile and a grey zone to completely exclude any false positive result. Specificity was confirmed by measuring levels of neutralizing antibodies against canine coronavirus, the avidity of specific antibodies, and their capacity to impede the binding of a recombinant RBD protein to VERO cells in an In-Cell ELISA. Sera from 464 cats and dogs sampled in 2020 and 2021 (“pandemic” samples) were assessed using the RBD-ELISA. Information on COVID-19 disease in the household and the animals’ lifestyles was collected. In Buenos Aires Suburbs cats were infected at a higher proportion than dogs, seroprevalence was 7.1 and 1.68%, respectively. Confirmed COVID-19 in the caregivers and outdoor lifestyle were statistically associated with seropositivity in cats. The risk of cats getting infected living indoors in COVID-19-negative households was null. The susceptibility of mammals to SARS-CoV-2, the possibility of transmission between animals themselves and humans, together with the free-roaming lifestyle typical of Buenos Aires suburban companion animals, urge pursuing responsible animal care and avoiding human interaction with animals during the disease course. The multi-species RBD-ELISA we developed can be used as a tool for serosurveillance of SARS-CoV-2 infection in mammalians (domestic and wild), guiding further targeted virological analyses to encounter susceptible species, interspecies transmission, and potential virus reservoirs in our region.
Fil: Cardoso, Nancy Patricia. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas.; Argentina
Fil: Rivero, Carla Ivanna. Universidad Nacional de Jose Clemente Paz. Instituto de Estudios Para El Desarrollo Productivo y la Innovacion.; Argentina
Fil: Castillo, Mariángeles. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas.; Argentina
Fil: Mansilla, Florencia Celeste. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas.; Argentina
Fil: Pastorino, Florencia Laura. Gobierno de la Provincia de Buenos Aires. Ministerio de Salud. Departamento de Zoonosis Urbanas.; Argentina
Fil: Piccirilli, Guadalupe. Gobierno de la Provincia de Buenos Aires. Ministerio de Salud. Departamento de Zoonosis Urbanas.; Argentina
Fil: Alonso, Laura. Gobierno de la Provincia de Buenos Aires. Ministerio de Salud. Departamento de Zoonosis Urbanas.; Argentina
Fil: Martínez, Gustavo. Gobierno de la Provincia de Buenos Aires. Ministerio de Salud. Departamento de Zoonosis Urbanas.; Argentina
Fil: Di Lullo, David. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet Noa Sur. Instituto Multidisciplinario de Salud, Tecnologia y Desarrollo. - Universidad Nacional de Santiago del Estero. Instituto Multidisciplinario de Salud, Tecnologia y Desarrollo.; Argentina
Fil: Bentancor, Leticia Veronica. Universidad Nacional de Jose Clemente Paz. Instituto de Estudios Para El Desarrollo Productivo y la Innovacion.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Capozzo, Alejandra Victoria. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas.; Argentina
Materia
COMPANION ANIMAL LIFESTYLE
COVID-19
MULTISPECIES SEROLOGY
RESPONSIBLE ANIMAL CARE
SEROEPIDEMIOLOGY STUDIES
TRANSMISSION DYNAMICS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/221593

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network_name_str CONICET Digital (CONICET)
spelling Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbsCardoso, Nancy PatriciaRivero, Carla IvannaCastillo, MariángelesMansilla, Florencia CelestePastorino, Florencia LauraPiccirilli, GuadalupeAlonso, LauraMartínez, GustavoDi Lullo, DavidBentancor, Leticia VeronicaCapozzo, Alejandra VictoriaCOMPANION ANIMAL LIFESTYLECOVID-19MULTISPECIES SEROLOGYRESPONSIBLE ANIMAL CARESEROEPIDEMIOLOGY STUDIESTRANSMISSION DYNAMICShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Coronavirus Disease 2019 (COVID-19) is a zoonotic disease caused by the pandemic virus SARS-CoV-2. Domestic and wild animals are susceptible to infection and are potential reservoirs for virus variants. To date, there is no information about the exposure of companion animals in Buenos Aires Suburbs, the area with the largest population in Argentina where the highest number of COVID-19 human cases occurred during the first infection wave. Here we developed a multi-species indirect ELISA to measure antibodies reactive to the SARS-CoV-2 receptor-binding domain (RBD) from several vertebrates constituting the class Mammalia, making it a valuable tool for field serosurveillance. The ELISA cut-off value was estimated by sera from dogs, cats, cattle, and pigs sampled before 2019 (n = 170), considering a 98% percentile and a grey zone to completely exclude any false positive result. Specificity was confirmed by measuring levels of neutralizing antibodies against canine coronavirus, the avidity of specific antibodies, and their capacity to impede the binding of a recombinant RBD protein to VERO cells in an In-Cell ELISA. Sera from 464 cats and dogs sampled in 2020 and 2021 (“pandemic” samples) were assessed using the RBD-ELISA. Information on COVID-19 disease in the household and the animals’ lifestyles was collected. In Buenos Aires Suburbs cats were infected at a higher proportion than dogs, seroprevalence was 7.1 and 1.68%, respectively. Confirmed COVID-19 in the caregivers and outdoor lifestyle were statistically associated with seropositivity in cats. The risk of cats getting infected living indoors in COVID-19-negative households was null. The susceptibility of mammals to SARS-CoV-2, the possibility of transmission between animals themselves and humans, together with the free-roaming lifestyle typical of Buenos Aires suburban companion animals, urge pursuing responsible animal care and avoiding human interaction with animals during the disease course. The multi-species RBD-ELISA we developed can be used as a tool for serosurveillance of SARS-CoV-2 infection in mammalians (domestic and wild), guiding further targeted virological analyses to encounter susceptible species, interspecies transmission, and potential virus reservoirs in our region.Fil: Cardoso, Nancy Patricia. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas.; ArgentinaFil: Rivero, Carla Ivanna. Universidad Nacional de Jose Clemente Paz. Instituto de Estudios Para El Desarrollo Productivo y la Innovacion.; ArgentinaFil: Castillo, Mariángeles. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas.; ArgentinaFil: Mansilla, Florencia Celeste. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas.; ArgentinaFil: Pastorino, Florencia Laura. Gobierno de la Provincia de Buenos Aires. Ministerio de Salud. Departamento de Zoonosis Urbanas.; ArgentinaFil: Piccirilli, Guadalupe. Gobierno de la Provincia de Buenos Aires. Ministerio de Salud. Departamento de Zoonosis Urbanas.; ArgentinaFil: Alonso, Laura. Gobierno de la Provincia de Buenos Aires. Ministerio de Salud. Departamento de Zoonosis Urbanas.; ArgentinaFil: Martínez, Gustavo. Gobierno de la Provincia de Buenos Aires. Ministerio de Salud. Departamento de Zoonosis Urbanas.; ArgentinaFil: Di Lullo, David. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet Noa Sur. Instituto Multidisciplinario de Salud, Tecnologia y Desarrollo. - Universidad Nacional de Santiago del Estero. Instituto Multidisciplinario de Salud, Tecnologia y Desarrollo.; ArgentinaFil: Bentancor, Leticia Veronica. Universidad Nacional de Jose Clemente Paz. Instituto de Estudios Para El Desarrollo Productivo y la Innovacion.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Capozzo, Alejandra Victoria. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas.; ArgentinaFrontiers Media2023-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/221593Cardoso, Nancy Patricia; Rivero, Carla Ivanna; Castillo, Mariángeles; Mansilla, Florencia Celeste; Pastorino, Florencia Laura; et al.; Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs; Frontiers Media; Frontiers in Veterinary Science; 10; 5-2023; 1-102297-1769CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fvets.2023.1161820/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fvets.2023.1161820info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T10:26:45Zoai:ri.conicet.gov.ar:11336/221593instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 10:26:46.174CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs
title Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs
spellingShingle Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs
Cardoso, Nancy Patricia
COMPANION ANIMAL LIFESTYLE
COVID-19
MULTISPECIES SEROLOGY
RESPONSIBLE ANIMAL CARE
SEROEPIDEMIOLOGY STUDIES
TRANSMISSION DYNAMICS
title_short Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs
title_full Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs
title_fullStr Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs
title_full_unstemmed Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs
title_sort Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs
dc.creator.none.fl_str_mv Cardoso, Nancy Patricia
Rivero, Carla Ivanna
Castillo, Mariángeles
Mansilla, Florencia Celeste
Pastorino, Florencia Laura
Piccirilli, Guadalupe
Alonso, Laura
Martínez, Gustavo
Di Lullo, David
Bentancor, Leticia Veronica
Capozzo, Alejandra Victoria
author Cardoso, Nancy Patricia
author_facet Cardoso, Nancy Patricia
Rivero, Carla Ivanna
Castillo, Mariángeles
Mansilla, Florencia Celeste
Pastorino, Florencia Laura
Piccirilli, Guadalupe
Alonso, Laura
Martínez, Gustavo
Di Lullo, David
Bentancor, Leticia Veronica
Capozzo, Alejandra Victoria
author_role author
author2 Rivero, Carla Ivanna
Castillo, Mariángeles
Mansilla, Florencia Celeste
Pastorino, Florencia Laura
Piccirilli, Guadalupe
Alonso, Laura
Martínez, Gustavo
Di Lullo, David
Bentancor, Leticia Veronica
Capozzo, Alejandra Victoria
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv COMPANION ANIMAL LIFESTYLE
COVID-19
MULTISPECIES SEROLOGY
RESPONSIBLE ANIMAL CARE
SEROEPIDEMIOLOGY STUDIES
TRANSMISSION DYNAMICS
topic COMPANION ANIMAL LIFESTYLE
COVID-19
MULTISPECIES SEROLOGY
RESPONSIBLE ANIMAL CARE
SEROEPIDEMIOLOGY STUDIES
TRANSMISSION DYNAMICS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The Coronavirus Disease 2019 (COVID-19) is a zoonotic disease caused by the pandemic virus SARS-CoV-2. Domestic and wild animals are susceptible to infection and are potential reservoirs for virus variants. To date, there is no information about the exposure of companion animals in Buenos Aires Suburbs, the area with the largest population in Argentina where the highest number of COVID-19 human cases occurred during the first infection wave. Here we developed a multi-species indirect ELISA to measure antibodies reactive to the SARS-CoV-2 receptor-binding domain (RBD) from several vertebrates constituting the class Mammalia, making it a valuable tool for field serosurveillance. The ELISA cut-off value was estimated by sera from dogs, cats, cattle, and pigs sampled before 2019 (n = 170), considering a 98% percentile and a grey zone to completely exclude any false positive result. Specificity was confirmed by measuring levels of neutralizing antibodies against canine coronavirus, the avidity of specific antibodies, and their capacity to impede the binding of a recombinant RBD protein to VERO cells in an In-Cell ELISA. Sera from 464 cats and dogs sampled in 2020 and 2021 (“pandemic” samples) were assessed using the RBD-ELISA. Information on COVID-19 disease in the household and the animals’ lifestyles was collected. In Buenos Aires Suburbs cats were infected at a higher proportion than dogs, seroprevalence was 7.1 and 1.68%, respectively. Confirmed COVID-19 in the caregivers and outdoor lifestyle were statistically associated with seropositivity in cats. The risk of cats getting infected living indoors in COVID-19-negative households was null. The susceptibility of mammals to SARS-CoV-2, the possibility of transmission between animals themselves and humans, together with the free-roaming lifestyle typical of Buenos Aires suburban companion animals, urge pursuing responsible animal care and avoiding human interaction with animals during the disease course. The multi-species RBD-ELISA we developed can be used as a tool for serosurveillance of SARS-CoV-2 infection in mammalians (domestic and wild), guiding further targeted virological analyses to encounter susceptible species, interspecies transmission, and potential virus reservoirs in our region.
Fil: Cardoso, Nancy Patricia. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas.; Argentina
Fil: Rivero, Carla Ivanna. Universidad Nacional de Jose Clemente Paz. Instituto de Estudios Para El Desarrollo Productivo y la Innovacion.; Argentina
Fil: Castillo, Mariángeles. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas.; Argentina
Fil: Mansilla, Florencia Celeste. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas.; Argentina
Fil: Pastorino, Florencia Laura. Gobierno de la Provincia de Buenos Aires. Ministerio de Salud. Departamento de Zoonosis Urbanas.; Argentina
Fil: Piccirilli, Guadalupe. Gobierno de la Provincia de Buenos Aires. Ministerio de Salud. Departamento de Zoonosis Urbanas.; Argentina
Fil: Alonso, Laura. Gobierno de la Provincia de Buenos Aires. Ministerio de Salud. Departamento de Zoonosis Urbanas.; Argentina
Fil: Martínez, Gustavo. Gobierno de la Provincia de Buenos Aires. Ministerio de Salud. Departamento de Zoonosis Urbanas.; Argentina
Fil: Di Lullo, David. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet Noa Sur. Instituto Multidisciplinario de Salud, Tecnologia y Desarrollo. - Universidad Nacional de Santiago del Estero. Instituto Multidisciplinario de Salud, Tecnologia y Desarrollo.; Argentina
Fil: Bentancor, Leticia Veronica. Universidad Nacional de Jose Clemente Paz. Instituto de Estudios Para El Desarrollo Productivo y la Innovacion.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Capozzo, Alejandra Victoria. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas.; Argentina
description The Coronavirus Disease 2019 (COVID-19) is a zoonotic disease caused by the pandemic virus SARS-CoV-2. Domestic and wild animals are susceptible to infection and are potential reservoirs for virus variants. To date, there is no information about the exposure of companion animals in Buenos Aires Suburbs, the area with the largest population in Argentina where the highest number of COVID-19 human cases occurred during the first infection wave. Here we developed a multi-species indirect ELISA to measure antibodies reactive to the SARS-CoV-2 receptor-binding domain (RBD) from several vertebrates constituting the class Mammalia, making it a valuable tool for field serosurveillance. The ELISA cut-off value was estimated by sera from dogs, cats, cattle, and pigs sampled before 2019 (n = 170), considering a 98% percentile and a grey zone to completely exclude any false positive result. Specificity was confirmed by measuring levels of neutralizing antibodies against canine coronavirus, the avidity of specific antibodies, and their capacity to impede the binding of a recombinant RBD protein to VERO cells in an In-Cell ELISA. Sera from 464 cats and dogs sampled in 2020 and 2021 (“pandemic” samples) were assessed using the RBD-ELISA. Information on COVID-19 disease in the household and the animals’ lifestyles was collected. In Buenos Aires Suburbs cats were infected at a higher proportion than dogs, seroprevalence was 7.1 and 1.68%, respectively. Confirmed COVID-19 in the caregivers and outdoor lifestyle were statistically associated with seropositivity in cats. The risk of cats getting infected living indoors in COVID-19-negative households was null. The susceptibility of mammals to SARS-CoV-2, the possibility of transmission between animals themselves and humans, together with the free-roaming lifestyle typical of Buenos Aires suburban companion animals, urge pursuing responsible animal care and avoiding human interaction with animals during the disease course. The multi-species RBD-ELISA we developed can be used as a tool for serosurveillance of SARS-CoV-2 infection in mammalians (domestic and wild), guiding further targeted virological analyses to encounter susceptible species, interspecies transmission, and potential virus reservoirs in our region.
publishDate 2023
dc.date.none.fl_str_mv 2023-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/221593
Cardoso, Nancy Patricia; Rivero, Carla Ivanna; Castillo, Mariángeles; Mansilla, Florencia Celeste; Pastorino, Florencia Laura; et al.; Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs; Frontiers Media; Frontiers in Veterinary Science; 10; 5-2023; 1-10
2297-1769
CONICET Digital
CONICET
url http://hdl.handle.net/11336/221593
identifier_str_mv Cardoso, Nancy Patricia; Rivero, Carla Ivanna; Castillo, Mariángeles; Mansilla, Florencia Celeste; Pastorino, Florencia Laura; et al.; Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs; Frontiers Media; Frontiers in Veterinary Science; 10; 5-2023; 1-10
2297-1769
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.3389/fvets.2023.1161820
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
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reponame_str CONICET Digital (CONICET)
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