Enzootic bovine leukosis: development of an indirect enzyme linked immunosorbent assay (I-ELISA) in seroepidemiological studies

Autores
González, Ester Teresa; Bonzo, Estela Beatriz; Echeverría, María Gabriela; Licursi, María; Etcheverrigaray, María Elisa
Año de publicación
1999
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bovine Leukemia Virus (BLV) is the etiologic agent of Enzootic Bovine Leukosis, a retrovirus exogenous to the bovine species. Once infected, there is no detectable viraemia but instead there is a strong and persistent immunological response to BLV structural proteins, essentially the gp51 envelope glycoprotein and the mayor core protein p24. We describe the test procedure of an indirect ELISA (I-ELISA) using polyclonal reagents for the detection of antibodies to BLV. For comparison, the sera were simultaneously tested by agar gel immunodiffussion (AGID) test, which is currently used as diagnostic standard for BLV infection. The antigen applied does not require a high degree of purification and the data from the analysis of the negative sera showed that the establishment of a cut-off level corresponding to 3 times the standard deviation (SD) above the mean for the negative control set of sera provided acceptable specificity, reducing the risk of false positives results. A comparison of the results obtained by AGID test and I-ELISA showed that considering a total of 465 serum samples, all of the 234 samples (50%) that were positive by AGID were positive to the I-ELISA. Of 225 serum samples which yielded negative results in the AGID test, 69 (15%) were found to be positive by the I-ELISA and 156 (33%) were negative by both techniques. Few sera (2%) that were non-specific by AGID were defined as negative or positive by I-ELISA.
Facultad de Ciencias Veterinarias
Materia
Ciencias Veterinarias
Bovine Leukaemia
indirect ELISA
seroepidemiology
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/115089

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network_name_str SEDICI (UNLP)
spelling Enzootic bovine leukosis: development of an indirect enzyme linked immunosorbent assay (I-ELISA) in seroepidemiological studiesGonzález, Ester TeresaBonzo, Estela BeatrizEcheverría, María GabrielaLicursi, MaríaEtcheverrigaray, María ElisaCiencias VeterinariasBovine Leukaemiaindirect ELISAseroepidemiologyBovine Leukemia Virus (BLV) is the etiologic agent of Enzootic Bovine Leukosis, a retrovirus exogenous to the bovine species. Once infected, there is no detectable viraemia but instead there is a strong and persistent immunological response to BLV structural proteins, essentially the gp51 envelope glycoprotein and the mayor core protein p24. We describe the test procedure of an indirect ELISA (I-ELISA) using polyclonal reagents for the detection of antibodies to BLV. For comparison, the sera were simultaneously tested by agar gel immunodiffussion (AGID) test, which is currently used as diagnostic standard for BLV infection. The antigen applied does not require a high degree of purification and the data from the analysis of the negative sera showed that the establishment of a cut-off level corresponding to 3 times the standard deviation (SD) above the mean for the negative control set of sera provided acceptable specificity, reducing the risk of false positives results. A comparison of the results obtained by AGID test and I-ELISA showed that considering a total of 465 serum samples, all of the 234 samples (50%) that were positive by AGID were positive to the I-ELISA. Of 225 serum samples which yielded negative results in the AGID test, 69 (15%) were found to be positive by the I-ELISA and 156 (33%) were negative by both techniques. Few sera (2%) that were non-specific by AGID were defined as negative or positive by I-ELISA.Facultad de Ciencias Veterinarias1999info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf37-42http://sedici.unlp.edu.ar/handle/10915/115089enginfo:eu-repo/semantics/altIdentifier/issn/0001-3714info:eu-repo/semantics/altIdentifier/doi/10.1590/S0001-37141999000100007info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/4.0/Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:58:58Zoai:sedici.unlp.edu.ar:10915/115089Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:58:58.786SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Enzootic bovine leukosis: development of an indirect enzyme linked immunosorbent assay (I-ELISA) in seroepidemiological studies
title Enzootic bovine leukosis: development of an indirect enzyme linked immunosorbent assay (I-ELISA) in seroepidemiological studies
spellingShingle Enzootic bovine leukosis: development of an indirect enzyme linked immunosorbent assay (I-ELISA) in seroepidemiological studies
González, Ester Teresa
Ciencias Veterinarias
Bovine Leukaemia
indirect ELISA
seroepidemiology
title_short Enzootic bovine leukosis: development of an indirect enzyme linked immunosorbent assay (I-ELISA) in seroepidemiological studies
title_full Enzootic bovine leukosis: development of an indirect enzyme linked immunosorbent assay (I-ELISA) in seroepidemiological studies
title_fullStr Enzootic bovine leukosis: development of an indirect enzyme linked immunosorbent assay (I-ELISA) in seroepidemiological studies
title_full_unstemmed Enzootic bovine leukosis: development of an indirect enzyme linked immunosorbent assay (I-ELISA) in seroepidemiological studies
title_sort Enzootic bovine leukosis: development of an indirect enzyme linked immunosorbent assay (I-ELISA) in seroepidemiological studies
dc.creator.none.fl_str_mv González, Ester Teresa
Bonzo, Estela Beatriz
Echeverría, María Gabriela
Licursi, María
Etcheverrigaray, María Elisa
author González, Ester Teresa
author_facet González, Ester Teresa
Bonzo, Estela Beatriz
Echeverría, María Gabriela
Licursi, María
Etcheverrigaray, María Elisa
author_role author
author2 Bonzo, Estela Beatriz
Echeverría, María Gabriela
Licursi, María
Etcheverrigaray, María Elisa
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Bovine Leukaemia
indirect ELISA
seroepidemiology
topic Ciencias Veterinarias
Bovine Leukaemia
indirect ELISA
seroepidemiology
dc.description.none.fl_txt_mv Bovine Leukemia Virus (BLV) is the etiologic agent of Enzootic Bovine Leukosis, a retrovirus exogenous to the bovine species. Once infected, there is no detectable viraemia but instead there is a strong and persistent immunological response to BLV structural proteins, essentially the gp51 envelope glycoprotein and the mayor core protein p24. We describe the test procedure of an indirect ELISA (I-ELISA) using polyclonal reagents for the detection of antibodies to BLV. For comparison, the sera were simultaneously tested by agar gel immunodiffussion (AGID) test, which is currently used as diagnostic standard for BLV infection. The antigen applied does not require a high degree of purification and the data from the analysis of the negative sera showed that the establishment of a cut-off level corresponding to 3 times the standard deviation (SD) above the mean for the negative control set of sera provided acceptable specificity, reducing the risk of false positives results. A comparison of the results obtained by AGID test and I-ELISA showed that considering a total of 465 serum samples, all of the 234 samples (50%) that were positive by AGID were positive to the I-ELISA. Of 225 serum samples which yielded negative results in the AGID test, 69 (15%) were found to be positive by the I-ELISA and 156 (33%) were negative by both techniques. Few sera (2%) that were non-specific by AGID were defined as negative or positive by I-ELISA.
Facultad de Ciencias Veterinarias
description Bovine Leukemia Virus (BLV) is the etiologic agent of Enzootic Bovine Leukosis, a retrovirus exogenous to the bovine species. Once infected, there is no detectable viraemia but instead there is a strong and persistent immunological response to BLV structural proteins, essentially the gp51 envelope glycoprotein and the mayor core protein p24. We describe the test procedure of an indirect ELISA (I-ELISA) using polyclonal reagents for the detection of antibodies to BLV. For comparison, the sera were simultaneously tested by agar gel immunodiffussion (AGID) test, which is currently used as diagnostic standard for BLV infection. The antigen applied does not require a high degree of purification and the data from the analysis of the negative sera showed that the establishment of a cut-off level corresponding to 3 times the standard deviation (SD) above the mean for the negative control set of sera provided acceptable specificity, reducing the risk of false positives results. A comparison of the results obtained by AGID test and I-ELISA showed that considering a total of 465 serum samples, all of the 234 samples (50%) that were positive by AGID were positive to the I-ELISA. Of 225 serum samples which yielded negative results in the AGID test, 69 (15%) were found to be positive by the I-ELISA and 156 (33%) were negative by both techniques. Few sera (2%) that were non-specific by AGID were defined as negative or positive by I-ELISA.
publishDate 1999
dc.date.none.fl_str_mv 1999
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
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format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/115089
url http://sedici.unlp.edu.ar/handle/10915/115089
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0001-3714
info:eu-repo/semantics/altIdentifier/doi/10.1590/S0001-37141999000100007
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc/4.0/
Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0/
Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
dc.format.none.fl_str_mv application/pdf
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