Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs

Autores
Cardoso, Nancy; Rivero, Carla; Castillo, Mariangeles; Mansilla, Florencia Celeste; Pastorino, Florencia; Piccirilli, Guadalupe; Alonso, Laura; Martinez, Gustavo; Di Lullo, David; Bentancor, Leticia Veronica; Capozzo, Alejandra
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The Coronavirus Disease 2019 (COVID-19) is a zoonotic disease caused by the pandemic virus SARS-CoV-2. Domestic and wild animals are susceptible to infection and are potential reservoirs for virus variants. To date, there is no information about the exposure of companion animals in Buenos Aires Suburbs, the area with the largest population in Argentina where the highest number of COVID-19 human cases occurred during the first infection wave. Here we developed a multi-species indirect ELISA to measure antibodies reactive to the SARS-CoV-2 receptor-binding domain (RBD) from several vertebrates constituting the class Mammalia, making it a valuable tool for field serosurveillance. The ELISA cut-off value was estimated by sera from dogs, cats, cattle, and pigs sampled before 2019 (n = 170), considering a 98% percentile and a grey zone to completely exclude any false positive result. Specificity was confirmed by measuring levels of neutralizing antibodies against canine coronavirus, the avidity of specific antibodies, and their capacity to impede the binding of a recombinant RBD protein to VERO cells in an In-Cell ELISA. Sera from 464 cats and dogs sampled in 2020 and 2021 (“pandemic” samples) were assessed using the RBD-ELISA. Information on COVID-19 disease in the household and the animals’ lifestyles was collected. In Buenos Aires Suburbs cats were infected at a higher proportion than dogs, seroprevalence was 7.1 and 1.68%, respectively. Confirmed COVID-19 in the caregivers and outdoor lifestyle were statistically associated with seropositivity in cats. The risk of cats getting infected living indoors in COVID-19-negative households was null. The susceptibility of mammals to SARS-CoV-2, the possibility of transmission between animals themselves and humans, together with the free-roaming lifestyle typical of Buenos Aires suburban companion animals, urge pursuing responsible animal care and avoiding human interaction with animals during the disease course. The multi-species RBD-ELISA we developed can be used as a tool for serosurveillance of SARS-CoV-2 infection in mammalians (domestic and wild), guiding further targeted virological analyses to encounter susceptible species, interspecies transmission, and potential virus reservoirs in our region.
Instituto de Virología
Fil: Cardoso, Nancy Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Cardoso, Nancy Patricia. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Fil: Rivero, Carla. Universidad Nacional de José Clemente Paz. Instituto de Estudios para el Desarrollo Productivo y la Innovación; Argentina
Fil: Castillo, Mariangeles. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Castillo, Mariangeles. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Fil: Mansilla, Florencia Celeste. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina.
Fil: Pastorino, Florencia. Provincia de Buenos Aires. Ministerio de Salud. Departamento Zoonosis Urbanas; Argentina
Fil: Piccirilli, Guadalupe. Provincia de Buenos Aires. Ministerio de Salud. Departamento Zoonosis Urbanas; Argentina
Fil: Alonso, Maria Laura. Provincia de Buenos Aires. Ministerio de Salud. Departamento Zoonosis Urbanas; Argentina
Fil: Martinez, Gustavo. Provincia de Buenos Aires. Ministerio de Salud. Departamento Zoonosis Urbanas; Argentina
Fil: Di Lullo, David. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Salud, Tecnología y Desarrollo; Argentina
Fil: Di Lullo, David. Universidad Nacional de Santiago del Estero. Instituto Multidisciplinario de Salud, Tecnología y Desarrollo; Argentina
Fil: Bentancor, Leticia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bentancor, Leticia Veronica. Universidad Nacional de José Clemente Paz. Instituto de Estudios para el Desarrollo Productivo y la Innovación; Argentina
Fil: Capozzo, Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Capozzo, Alejandra Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Frontiers in Veterinary Science 10 : 1161820. (May 2023)
Materia
COVID-19
Severe Acute Respiratory Syndrome Coronavirus 2
Domestic Animals
Immunodiagnosis
ELISA
Screening
Coronavirus del Síndrome Respiratorio Agudo Grave 2
Animales Domésticos
Inmunodiagnóstico
Evaluación Preliminar
SARS-CoV-2
Suburbios de Buenos Aires
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/14703
Acceder
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network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbsCardoso, NancyRivero, CarlaCastillo, MariangelesMansilla, Florencia CelestePastorino, FlorenciaPiccirilli, GuadalupeAlonso, LauraMartinez, GustavoDi Lullo, DavidBentancor, Leticia VeronicaCapozzo, AlejandraCOVID-19Severe Acute Respiratory Syndrome Coronavirus 2Domestic AnimalsImmunodiagnosisELISAScreeningCoronavirus del Síndrome Respiratorio Agudo Grave 2Animales DomésticosInmunodiagnósticoEvaluación PreliminarSARS-CoV-2Suburbios de Buenos AiresThe Coronavirus Disease 2019 (COVID-19) is a zoonotic disease caused by the pandemic virus SARS-CoV-2. Domestic and wild animals are susceptible to infection and are potential reservoirs for virus variants. To date, there is no information about the exposure of companion animals in Buenos Aires Suburbs, the area with the largest population in Argentina where the highest number of COVID-19 human cases occurred during the first infection wave. Here we developed a multi-species indirect ELISA to measure antibodies reactive to the SARS-CoV-2 receptor-binding domain (RBD) from several vertebrates constituting the class Mammalia, making it a valuable tool for field serosurveillance. The ELISA cut-off value was estimated by sera from dogs, cats, cattle, and pigs sampled before 2019 (n = 170), considering a 98% percentile and a grey zone to completely exclude any false positive result. Specificity was confirmed by measuring levels of neutralizing antibodies against canine coronavirus, the avidity of specific antibodies, and their capacity to impede the binding of a recombinant RBD protein to VERO cells in an In-Cell ELISA. Sera from 464 cats and dogs sampled in 2020 and 2021 (“pandemic” samples) were assessed using the RBD-ELISA. Information on COVID-19 disease in the household and the animals’ lifestyles was collected. In Buenos Aires Suburbs cats were infected at a higher proportion than dogs, seroprevalence was 7.1 and 1.68%, respectively. Confirmed COVID-19 in the caregivers and outdoor lifestyle were statistically associated with seropositivity in cats. The risk of cats getting infected living indoors in COVID-19-negative households was null. The susceptibility of mammals to SARS-CoV-2, the possibility of transmission between animals themselves and humans, together with the free-roaming lifestyle typical of Buenos Aires suburban companion animals, urge pursuing responsible animal care and avoiding human interaction with animals during the disease course. The multi-species RBD-ELISA we developed can be used as a tool for serosurveillance of SARS-CoV-2 infection in mammalians (domestic and wild), guiding further targeted virological analyses to encounter susceptible species, interspecies transmission, and potential virus reservoirs in our region.Instituto de VirologíaFil: Cardoso, Nancy Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Cardoso, Nancy Patricia. Consejo Nacional de Investigaciones Científicas y Tecnológicas; ArgentinaFil: Rivero, Carla. Universidad Nacional de José Clemente Paz. Instituto de Estudios para el Desarrollo Productivo y la Innovación; ArgentinaFil: Castillo, Mariangeles. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Castillo, Mariangeles. Consejo Nacional de Investigaciones Científicas y Tecnológicas; ArgentinaFil: Mansilla, Florencia Celeste. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina.Fil: Pastorino, Florencia. Provincia de Buenos Aires. Ministerio de Salud. Departamento Zoonosis Urbanas; ArgentinaFil: Piccirilli, Guadalupe. Provincia de Buenos Aires. Ministerio de Salud. Departamento Zoonosis Urbanas; ArgentinaFil: Alonso, Maria Laura. Provincia de Buenos Aires. Ministerio de Salud. Departamento Zoonosis Urbanas; ArgentinaFil: Martinez, Gustavo. Provincia de Buenos Aires. Ministerio de Salud. Departamento Zoonosis Urbanas; ArgentinaFil: Di Lullo, David. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Salud, Tecnología y Desarrollo; ArgentinaFil: Di Lullo, David. Universidad Nacional de Santiago del Estero. Instituto Multidisciplinario de Salud, Tecnología y Desarrollo; ArgentinaFil: Bentancor, Leticia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bentancor, Leticia Veronica. Universidad Nacional de José Clemente Paz. Instituto de Estudios para el Desarrollo Productivo y la Innovación; ArgentinaFil: Capozzo, Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Capozzo, Alejandra Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFrontiers Media2023-07-05T17:42:07Z2023-07-05T17:42:07Z2023-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/14703https://www.frontiersin.org/articles/10.3389/fvets.2023.1161820/full2297-1769https://doi.org/10.3389/fvets.2023.1161820Frontiers in Veterinary Science 10 : 1161820. (May 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E5-I105-001/2019-PD-E5-I105-001, Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco ?Una Salud?Greater Buenos Aires .......... (general region) (World, South America, Argentina)8725264info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:31:12Zoai:localhost:20.500.12123/14703instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:31:12.358INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs
title Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs
spellingShingle Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs
Cardoso, Nancy
COVID-19
Severe Acute Respiratory Syndrome Coronavirus 2
Domestic Animals
Immunodiagnosis
ELISA
Screening
Coronavirus del Síndrome Respiratorio Agudo Grave 2
Animales Domésticos
Inmunodiagnóstico
Evaluación Preliminar
SARS-CoV-2
Suburbios de Buenos Aires
title_short Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs
title_full Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs
title_fullStr Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs
title_full_unstemmed Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs
title_sort Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs
dc.creator.none.fl_str_mv Cardoso, Nancy
Rivero, Carla
Castillo, Mariangeles
Mansilla, Florencia Celeste
Pastorino, Florencia
Piccirilli, Guadalupe
Alonso, Laura
Martinez, Gustavo
Di Lullo, David
Bentancor, Leticia Veronica
Capozzo, Alejandra
author Cardoso, Nancy
author_facet Cardoso, Nancy
Rivero, Carla
Castillo, Mariangeles
Mansilla, Florencia Celeste
Pastorino, Florencia
Piccirilli, Guadalupe
Alonso, Laura
Martinez, Gustavo
Di Lullo, David
Bentancor, Leticia Veronica
Capozzo, Alejandra
author_role author
author2 Rivero, Carla
Castillo, Mariangeles
Mansilla, Florencia Celeste
Pastorino, Florencia
Piccirilli, Guadalupe
Alonso, Laura
Martinez, Gustavo
Di Lullo, David
Bentancor, Leticia Veronica
Capozzo, Alejandra
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv COVID-19
Severe Acute Respiratory Syndrome Coronavirus 2
Domestic Animals
Immunodiagnosis
ELISA
Screening
Coronavirus del Síndrome Respiratorio Agudo Grave 2
Animales Domésticos
Inmunodiagnóstico
Evaluación Preliminar
SARS-CoV-2
Suburbios de Buenos Aires
topic COVID-19
Severe Acute Respiratory Syndrome Coronavirus 2
Domestic Animals
Immunodiagnosis
ELISA
Screening
Coronavirus del Síndrome Respiratorio Agudo Grave 2
Animales Domésticos
Inmunodiagnóstico
Evaluación Preliminar
SARS-CoV-2
Suburbios de Buenos Aires
dc.description.none.fl_txt_mv The Coronavirus Disease 2019 (COVID-19) is a zoonotic disease caused by the pandemic virus SARS-CoV-2. Domestic and wild animals are susceptible to infection and are potential reservoirs for virus variants. To date, there is no information about the exposure of companion animals in Buenos Aires Suburbs, the area with the largest population in Argentina where the highest number of COVID-19 human cases occurred during the first infection wave. Here we developed a multi-species indirect ELISA to measure antibodies reactive to the SARS-CoV-2 receptor-binding domain (RBD) from several vertebrates constituting the class Mammalia, making it a valuable tool for field serosurveillance. The ELISA cut-off value was estimated by sera from dogs, cats, cattle, and pigs sampled before 2019 (n = 170), considering a 98% percentile and a grey zone to completely exclude any false positive result. Specificity was confirmed by measuring levels of neutralizing antibodies against canine coronavirus, the avidity of specific antibodies, and their capacity to impede the binding of a recombinant RBD protein to VERO cells in an In-Cell ELISA. Sera from 464 cats and dogs sampled in 2020 and 2021 (“pandemic” samples) were assessed using the RBD-ELISA. Information on COVID-19 disease in the household and the animals’ lifestyles was collected. In Buenos Aires Suburbs cats were infected at a higher proportion than dogs, seroprevalence was 7.1 and 1.68%, respectively. Confirmed COVID-19 in the caregivers and outdoor lifestyle were statistically associated with seropositivity in cats. The risk of cats getting infected living indoors in COVID-19-negative households was null. The susceptibility of mammals to SARS-CoV-2, the possibility of transmission between animals themselves and humans, together with the free-roaming lifestyle typical of Buenos Aires suburban companion animals, urge pursuing responsible animal care and avoiding human interaction with animals during the disease course. The multi-species RBD-ELISA we developed can be used as a tool for serosurveillance of SARS-CoV-2 infection in mammalians (domestic and wild), guiding further targeted virological analyses to encounter susceptible species, interspecies transmission, and potential virus reservoirs in our region.
Instituto de Virología
Fil: Cardoso, Nancy Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Cardoso, Nancy Patricia. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Fil: Rivero, Carla. Universidad Nacional de José Clemente Paz. Instituto de Estudios para el Desarrollo Productivo y la Innovación; Argentina
Fil: Castillo, Mariangeles. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Castillo, Mariangeles. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Fil: Mansilla, Florencia Celeste. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina.
Fil: Pastorino, Florencia. Provincia de Buenos Aires. Ministerio de Salud. Departamento Zoonosis Urbanas; Argentina
Fil: Piccirilli, Guadalupe. Provincia de Buenos Aires. Ministerio de Salud. Departamento Zoonosis Urbanas; Argentina
Fil: Alonso, Maria Laura. Provincia de Buenos Aires. Ministerio de Salud. Departamento Zoonosis Urbanas; Argentina
Fil: Martinez, Gustavo. Provincia de Buenos Aires. Ministerio de Salud. Departamento Zoonosis Urbanas; Argentina
Fil: Di Lullo, David. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Salud, Tecnología y Desarrollo; Argentina
Fil: Di Lullo, David. Universidad Nacional de Santiago del Estero. Instituto Multidisciplinario de Salud, Tecnología y Desarrollo; Argentina
Fil: Bentancor, Leticia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bentancor, Leticia Veronica. Universidad Nacional de José Clemente Paz. Instituto de Estudios para el Desarrollo Productivo y la Innovación; Argentina
Fil: Capozzo, Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Capozzo, Alejandra Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The Coronavirus Disease 2019 (COVID-19) is a zoonotic disease caused by the pandemic virus SARS-CoV-2. Domestic and wild animals are susceptible to infection and are potential reservoirs for virus variants. To date, there is no information about the exposure of companion animals in Buenos Aires Suburbs, the area with the largest population in Argentina where the highest number of COVID-19 human cases occurred during the first infection wave. Here we developed a multi-species indirect ELISA to measure antibodies reactive to the SARS-CoV-2 receptor-binding domain (RBD) from several vertebrates constituting the class Mammalia, making it a valuable tool for field serosurveillance. The ELISA cut-off value was estimated by sera from dogs, cats, cattle, and pigs sampled before 2019 (n = 170), considering a 98% percentile and a grey zone to completely exclude any false positive result. Specificity was confirmed by measuring levels of neutralizing antibodies against canine coronavirus, the avidity of specific antibodies, and their capacity to impede the binding of a recombinant RBD protein to VERO cells in an In-Cell ELISA. Sera from 464 cats and dogs sampled in 2020 and 2021 (“pandemic” samples) were assessed using the RBD-ELISA. Information on COVID-19 disease in the household and the animals’ lifestyles was collected. In Buenos Aires Suburbs cats were infected at a higher proportion than dogs, seroprevalence was 7.1 and 1.68%, respectively. Confirmed COVID-19 in the caregivers and outdoor lifestyle were statistically associated with seropositivity in cats. The risk of cats getting infected living indoors in COVID-19-negative households was null. The susceptibility of mammals to SARS-CoV-2, the possibility of transmission between animals themselves and humans, together with the free-roaming lifestyle typical of Buenos Aires suburban companion animals, urge pursuing responsible animal care and avoiding human interaction with animals during the disease course. The multi-species RBD-ELISA we developed can be used as a tool for serosurveillance of SARS-CoV-2 infection in mammalians (domestic and wild), guiding further targeted virological analyses to encounter susceptible species, interspecies transmission, and potential virus reservoirs in our region.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-05T17:42:07Z
2023-07-05T17:42:07Z
2023-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/14703
https://www.frontiersin.org/articles/10.3389/fvets.2023.1161820/full
2297-1769
https://doi.org/10.3389/fvets.2023.1161820
url http://hdl.handle.net/20.500.12123/14703
https://www.frontiersin.org/articles/10.3389/fvets.2023.1161820/full
https://doi.org/10.3389/fvets.2023.1161820
identifier_str_mv 2297-1769
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E5-I105-001/2019-PD-E5-I105-001, Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco ?Una Salud?
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
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rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Greater Buenos Aires .......... (general region) (World, South America, Argentina)
8725264
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Frontiers in Veterinary Science 10 : 1161820. (May 2023)
reponame:INTA Digital (INTA)
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instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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