High affinity receptors but not low affinity receptors for neurotensin are involved in neuronal Na/K-ATPase inhibition by the peptide

Autores
Alvarez Juliá, Anabel; Gutnisky, Alicia; López Ordieres, María Graciela; Rodriguez, Georgina Emma
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Neurotensin acts as a neuromodulator or as a neurotransmitter that binds to a group of receptors. Two of the receptors, namely NTS1 and NTS2, bind to neurotensin with high affinity and low affinity, respectively. Neurotensin added in vitro inhibits synaptosomal membrane Na+ , K+ - ATPase activity. This effect seems to be mediated by NTS1 receptor because it is fully blocked by antagonist SR 48692. Herein neurotensin effect was assayed after administration of SR 48692 and levocabastine which are antagonists for NTS1 and NTS2 receptors, respectively. Male Wistar rats were administered by i.p. injection, with 150 µg/kg SR 48692 (Sanofi-Aventis U.S., Inc.) suspended in the vehicle (0.01% Tween 80 in saline solution), 50 μg/kg levocabastine (disolved in saline solution) and the corresponding vehicle solutions. Thirty minutes later, the animals were sacrificed, cerebral cortices removed, separately pooled, and processed to obtain synaptosomal membranes. In membrane samples, Na+ , K+ - and Mg2+-ATPase activities were determined in the absence and presence of 3.5 x 10-6 M neurotensin. Basal Na+ , K+ -ATPase activity in membranes isolated from control rats (vehicle injected) decreased roughly by 60% by the peptide. This effect was entirely prevented by the administration of NTS1 antagonist SR 48692. Administration of levocabastine, which enhanced basal Na+ , K+ - ATPase activity, failed to prevent neurotensin inhibitory effect on this enzyme activity. Mg2+- ATPase activity remained unaltered in all conditions tested. It is concluded that Na+ , K+ - ATPase inhibition by neurotensin seems mediated only by NTS1 receptor because the administration of NTS1 antagonist SR 48692, and not the NTS2 antagonist levocabastine prevented the effect of the peptide.
Fil: Alvarez Juliá, Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia ; Argentina
Fil: Gutnisky, Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia ; Argentina
Fil: López Ordieres, María Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia ; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología. Cátedra de Farmacología; Argentina
Fil: Rodriguez, Georgina Emma. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia ; Argentina
Materia
Na+, Inhibition
Receptors
High Affinity
Neurotensin
K+-Atpase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/17155
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/17155
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling High affinity receptors but not low affinity receptors for neurotensin are involved in neuronal Na/K-ATPase inhibition by the peptideAlvarez Juliá, AnabelGutnisky, AliciaLópez Ordieres, María GracielaRodriguez, Georgina EmmaNa+, InhibitionReceptorsHigh AffinityNeurotensinK+-Atpasehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Neurotensin acts as a neuromodulator or as a neurotransmitter that binds to a group of receptors. Two of the receptors, namely NTS1 and NTS2, bind to neurotensin with high affinity and low affinity, respectively. Neurotensin added in vitro inhibits synaptosomal membrane Na+ , K+ - ATPase activity. This effect seems to be mediated by NTS1 receptor because it is fully blocked by antagonist SR 48692. Herein neurotensin effect was assayed after administration of SR 48692 and levocabastine which are antagonists for NTS1 and NTS2 receptors, respectively. Male Wistar rats were administered by i.p. injection, with 150 µg/kg SR 48692 (Sanofi-Aventis U.S., Inc.) suspended in the vehicle (0.01% Tween 80 in saline solution), 50 μg/kg levocabastine (disolved in saline solution) and the corresponding vehicle solutions. Thirty minutes later, the animals were sacrificed, cerebral cortices removed, separately pooled, and processed to obtain synaptosomal membranes. In membrane samples, Na+ , K+ - and Mg2+-ATPase activities were determined in the absence and presence of 3.5 x 10-6 M neurotensin. Basal Na+ , K+ -ATPase activity in membranes isolated from control rats (vehicle injected) decreased roughly by 60% by the peptide. This effect was entirely prevented by the administration of NTS1 antagonist SR 48692. Administration of levocabastine, which enhanced basal Na+ , K+ - ATPase activity, failed to prevent neurotensin inhibitory effect on this enzyme activity. Mg2+- ATPase activity remained unaltered in all conditions tested. It is concluded that Na+ , K+ - ATPase inhibition by neurotensin seems mediated only by NTS1 receptor because the administration of NTS1 antagonist SR 48692, and not the NTS2 antagonist levocabastine prevented the effect of the peptide.Fil: Alvarez Juliá, Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia ; ArgentinaFil: Gutnisky, Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia ; ArgentinaFil: López Ordieres, María Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia ; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología. Cátedra de Farmacología; ArgentinaFil: Rodriguez, Georgina Emma. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia ; ArgentinaResearch Trends2013-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/17155Alvarez Juliá, Anabel; Gutnisky, Alicia; López Ordieres, María Graciela; Rodriguez, Georgina Emma; High affinity receptors but not low affinity receptors for neurotensin are involved in neuronal Na/K-ATPase inhibition by the peptide; Research Trends; Current Topics in Peptide & Protein Research; 14; 7-2013; 27-320972-4524enginfo:eu-repo/semantics/altIdentifier/url/http://www.researchtrends.net/tia/abstract.asp?in=0&vn=14&tid=26&aid=5154&pub=2013&type=3info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:38:09Zoai:ri.conicet.gov.ar:11336/17155instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:38:09.463CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv High affinity receptors but not low affinity receptors for neurotensin are involved in neuronal Na/K-ATPase inhibition by the peptide
title High affinity receptors but not low affinity receptors for neurotensin are involved in neuronal Na/K-ATPase inhibition by the peptide
spellingShingle High affinity receptors but not low affinity receptors for neurotensin are involved in neuronal Na/K-ATPase inhibition by the peptide
Alvarez Juliá, Anabel
Na+, Inhibition
Receptors
High Affinity
Neurotensin
K+-Atpase
title_short High affinity receptors but not low affinity receptors for neurotensin are involved in neuronal Na/K-ATPase inhibition by the peptide
title_full High affinity receptors but not low affinity receptors for neurotensin are involved in neuronal Na/K-ATPase inhibition by the peptide
title_fullStr High affinity receptors but not low affinity receptors for neurotensin are involved in neuronal Na/K-ATPase inhibition by the peptide
title_full_unstemmed High affinity receptors but not low affinity receptors for neurotensin are involved in neuronal Na/K-ATPase inhibition by the peptide
title_sort High affinity receptors but not low affinity receptors for neurotensin are involved in neuronal Na/K-ATPase inhibition by the peptide
dc.creator.none.fl_str_mv Alvarez Juliá, Anabel
Gutnisky, Alicia
López Ordieres, María Graciela
Rodriguez, Georgina Emma
author Alvarez Juliá, Anabel
author_facet Alvarez Juliá, Anabel
Gutnisky, Alicia
López Ordieres, María Graciela
Rodriguez, Georgina Emma
author_role author
author2 Gutnisky, Alicia
López Ordieres, María Graciela
Rodriguez, Georgina Emma
author2_role author
author
author
dc.subject.none.fl_str_mv Na+, Inhibition
Receptors
High Affinity
Neurotensin
K+-Atpase
topic Na+, Inhibition
Receptors
High Affinity
Neurotensin
K+-Atpase
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Neurotensin acts as a neuromodulator or as a neurotransmitter that binds to a group of receptors. Two of the receptors, namely NTS1 and NTS2, bind to neurotensin with high affinity and low affinity, respectively. Neurotensin added in vitro inhibits synaptosomal membrane Na+ , K+ - ATPase activity. This effect seems to be mediated by NTS1 receptor because it is fully blocked by antagonist SR 48692. Herein neurotensin effect was assayed after administration of SR 48692 and levocabastine which are antagonists for NTS1 and NTS2 receptors, respectively. Male Wistar rats were administered by i.p. injection, with 150 µg/kg SR 48692 (Sanofi-Aventis U.S., Inc.) suspended in the vehicle (0.01% Tween 80 in saline solution), 50 μg/kg levocabastine (disolved in saline solution) and the corresponding vehicle solutions. Thirty minutes later, the animals were sacrificed, cerebral cortices removed, separately pooled, and processed to obtain synaptosomal membranes. In membrane samples, Na+ , K+ - and Mg2+-ATPase activities were determined in the absence and presence of 3.5 x 10-6 M neurotensin. Basal Na+ , K+ -ATPase activity in membranes isolated from control rats (vehicle injected) decreased roughly by 60% by the peptide. This effect was entirely prevented by the administration of NTS1 antagonist SR 48692. Administration of levocabastine, which enhanced basal Na+ , K+ - ATPase activity, failed to prevent neurotensin inhibitory effect on this enzyme activity. Mg2+- ATPase activity remained unaltered in all conditions tested. It is concluded that Na+ , K+ - ATPase inhibition by neurotensin seems mediated only by NTS1 receptor because the administration of NTS1 antagonist SR 48692, and not the NTS2 antagonist levocabastine prevented the effect of the peptide.
Fil: Alvarez Juliá, Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia ; Argentina
Fil: Gutnisky, Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia ; Argentina
Fil: López Ordieres, María Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia ; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología. Cátedra de Farmacología; Argentina
Fil: Rodriguez, Georgina Emma. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia ; Argentina
description Neurotensin acts as a neuromodulator or as a neurotransmitter that binds to a group of receptors. Two of the receptors, namely NTS1 and NTS2, bind to neurotensin with high affinity and low affinity, respectively. Neurotensin added in vitro inhibits synaptosomal membrane Na+ , K+ - ATPase activity. This effect seems to be mediated by NTS1 receptor because it is fully blocked by antagonist SR 48692. Herein neurotensin effect was assayed after administration of SR 48692 and levocabastine which are antagonists for NTS1 and NTS2 receptors, respectively. Male Wistar rats were administered by i.p. injection, with 150 µg/kg SR 48692 (Sanofi-Aventis U.S., Inc.) suspended in the vehicle (0.01% Tween 80 in saline solution), 50 μg/kg levocabastine (disolved in saline solution) and the corresponding vehicle solutions. Thirty minutes later, the animals were sacrificed, cerebral cortices removed, separately pooled, and processed to obtain synaptosomal membranes. In membrane samples, Na+ , K+ - and Mg2+-ATPase activities were determined in the absence and presence of 3.5 x 10-6 M neurotensin. Basal Na+ , K+ -ATPase activity in membranes isolated from control rats (vehicle injected) decreased roughly by 60% by the peptide. This effect was entirely prevented by the administration of NTS1 antagonist SR 48692. Administration of levocabastine, which enhanced basal Na+ , K+ - ATPase activity, failed to prevent neurotensin inhibitory effect on this enzyme activity. Mg2+- ATPase activity remained unaltered in all conditions tested. It is concluded that Na+ , K+ - ATPase inhibition by neurotensin seems mediated only by NTS1 receptor because the administration of NTS1 antagonist SR 48692, and not the NTS2 antagonist levocabastine prevented the effect of the peptide.
publishDate 2013
dc.date.none.fl_str_mv 2013-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/17155
Alvarez Juliá, Anabel; Gutnisky, Alicia; López Ordieres, María Graciela; Rodriguez, Georgina Emma; High affinity receptors but not low affinity receptors for neurotensin are involved in neuronal Na/K-ATPase inhibition by the peptide; Research Trends; Current Topics in Peptide & Protein Research; 14; 7-2013; 27-32
0972-4524
url http://hdl.handle.net/11336/17155
identifier_str_mv Alvarez Juliá, Anabel; Gutnisky, Alicia; López Ordieres, María Graciela; Rodriguez, Georgina Emma; High affinity receptors but not low affinity receptors for neurotensin are involved in neuronal Na/K-ATPase inhibition by the peptide; Research Trends; Current Topics in Peptide & Protein Research; 14; 7-2013; 27-32
0972-4524
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.researchtrends.net/tia/abstract.asp?in=0&vn=14&tid=26&aid=5154&pub=2013&type=3
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Research Trends
publisher.none.fl_str_mv Research Trends
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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