Neurotensin decreases high affinity [ 3H]-ouabain binding to cerebral cortex membranes

Autores
Rosin, Carina; López Ordieres, María Graciela; Rodriguez, Georgina Emma
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Previous work from this laboratory showed the ability of neurotensin to inhibit synaptosomal membrane Na +, K +-ATPase activity, the effect being blocked by SR 48692, a non-peptidic antagonist for high affinity neurotensin receptor (NTS1) [López Ordieres and Rodríguez de Lores Arnaiz 2000; 2001]. To further study neurotensin interaction with Na +, K +-ATPase, peptide effect on high affinity [ 3H]-ouabain binding was studied in cerebral cortex membranes. It was observed that neurotensin modified binding in a dose-dependent manner, leading to 80% decrease with 1×10 4M concentration. On the other hand, the single addition of 1×10 -6M, 1×10 5M and 1×10 M SR 48692 (Sanofi-Aventis, U.S., Inc.) decreased [ 3H]-ouabain binding (in %) to 87±16; 74±16 and 34±17, respectively. Simultaneous addition of neurotensin and SR 48692 led to additive or synergic effects. Partial NTS2 agonist levocabastine inhibited [ 3H]-ouabain binding likewise. Saturation assays followed by Scatchard analyses showed that neurotensin increased K d value whereas failed to modify B max value, indicating a competitive type interaction of the peptide at Na +, K +-ATPase ouabain site. At variance, SR 48692 decreased B max value whereas it did not modify K d value. [ 3H]-ouabain binding was also studied in cerebral cortex membranes obtained from rats injected i. p. 30min earlier with 100μg and 250μg/kg SR 48692. It was observed that the 250μg/kg SR 48692 dose led to 19% decrease in basal [ 3H]-ouabain binding. After SR 48692 treatments, addition of 1×10 6M led to additive or synergic effect. Results suggested that [ 3H]-ouabain binding inhibition by neurotensin hardly involves NTS1 receptor. © 2010 Elsevier B.V.
Fil: Rosin, Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología. Cátedra de Farmacología; Argentina
Fil: López Ordieres, María Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología. Cátedra de Farmacología; Argentina
Fil: Rodriguez, Georgina Emma. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología. Cátedra de Farmacología; Argentina
Materia
[ 3h]-Ouabain Binding
Cns Membranes
Neurotensin
Nts1 Receptor
Nts2 Receptor
Sr 48692
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/67552
Acceder
id CONICETDig_65aa919865c952b77387bc48c6645bcc
oai_identifier_str oai:ri.conicet.gov.ar:11336/67552
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Neurotensin decreases high affinity [ 3H]-ouabain binding to cerebral cortex membranesRosin, CarinaLópez Ordieres, María GracielaRodriguez, Georgina Emma[ 3h]-Ouabain BindingCns MembranesNeurotensinNts1 ReceptorNts2 ReceptorSr 48692https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Previous work from this laboratory showed the ability of neurotensin to inhibit synaptosomal membrane Na +, K +-ATPase activity, the effect being blocked by SR 48692, a non-peptidic antagonist for high affinity neurotensin receptor (NTS1) [López Ordieres and Rodríguez de Lores Arnaiz 2000; 2001]. To further study neurotensin interaction with Na +, K +-ATPase, peptide effect on high affinity [ 3H]-ouabain binding was studied in cerebral cortex membranes. It was observed that neurotensin modified binding in a dose-dependent manner, leading to 80% decrease with 1×10 4M concentration. On the other hand, the single addition of 1×10 -6M, 1×10 5M and 1×10 M SR 48692 (Sanofi-Aventis, U.S., Inc.) decreased [ 3H]-ouabain binding (in %) to 87±16; 74±16 and 34±17, respectively. Simultaneous addition of neurotensin and SR 48692 led to additive or synergic effects. Partial NTS2 agonist levocabastine inhibited [ 3H]-ouabain binding likewise. Saturation assays followed by Scatchard analyses showed that neurotensin increased K d value whereas failed to modify B max value, indicating a competitive type interaction of the peptide at Na +, K +-ATPase ouabain site. At variance, SR 48692 decreased B max value whereas it did not modify K d value. [ 3H]-ouabain binding was also studied in cerebral cortex membranes obtained from rats injected i. p. 30min earlier with 100μg and 250μg/kg SR 48692. It was observed that the 250μg/kg SR 48692 dose led to 19% decrease in basal [ 3H]-ouabain binding. After SR 48692 treatments, addition of 1×10 6M led to additive or synergic effect. Results suggested that [ 3H]-ouabain binding inhibition by neurotensin hardly involves NTS1 receptor. © 2010 Elsevier B.V.Fil: Rosin, Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología. Cátedra de Farmacología; ArgentinaFil: López Ordieres, María Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología. Cátedra de Farmacología; ArgentinaFil: Rodriguez, Georgina Emma. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología. Cátedra de Farmacología; ArgentinaElsevier Science2011-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/67552Rosin, Carina; López Ordieres, María Graciela; Rodriguez, Georgina Emma; Neurotensin decreases high affinity [ 3H]-ouabain binding to cerebral cortex membranes; Elsevier Science; Regulatory Peptides; 172; 1-3; 12-2011; 35-400167-0115CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.regpep.2011.08.004info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0167011511001522info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:22Zoai:ri.conicet.gov.ar:11336/67552instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:22.508CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Neurotensin decreases high affinity [ 3H]-ouabain binding to cerebral cortex membranes
title Neurotensin decreases high affinity [ 3H]-ouabain binding to cerebral cortex membranes
spellingShingle Neurotensin decreases high affinity [ 3H]-ouabain binding to cerebral cortex membranes
Rosin, Carina
[ 3h]-Ouabain Binding
Cns Membranes
Neurotensin
Nts1 Receptor
Nts2 Receptor
Sr 48692
title_short Neurotensin decreases high affinity [ 3H]-ouabain binding to cerebral cortex membranes
title_full Neurotensin decreases high affinity [ 3H]-ouabain binding to cerebral cortex membranes
title_fullStr Neurotensin decreases high affinity [ 3H]-ouabain binding to cerebral cortex membranes
title_full_unstemmed Neurotensin decreases high affinity [ 3H]-ouabain binding to cerebral cortex membranes
title_sort Neurotensin decreases high affinity [ 3H]-ouabain binding to cerebral cortex membranes
dc.creator.none.fl_str_mv Rosin, Carina
López Ordieres, María Graciela
Rodriguez, Georgina Emma
author Rosin, Carina
author_facet Rosin, Carina
López Ordieres, María Graciela
Rodriguez, Georgina Emma
author_role author
author2 López Ordieres, María Graciela
Rodriguez, Georgina Emma
author2_role author
author
dc.subject.none.fl_str_mv [ 3h]-Ouabain Binding
Cns Membranes
Neurotensin
Nts1 Receptor
Nts2 Receptor
Sr 48692
topic [ 3h]-Ouabain Binding
Cns Membranes
Neurotensin
Nts1 Receptor
Nts2 Receptor
Sr 48692
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Previous work from this laboratory showed the ability of neurotensin to inhibit synaptosomal membrane Na +, K +-ATPase activity, the effect being blocked by SR 48692, a non-peptidic antagonist for high affinity neurotensin receptor (NTS1) [López Ordieres and Rodríguez de Lores Arnaiz 2000; 2001]. To further study neurotensin interaction with Na +, K +-ATPase, peptide effect on high affinity [ 3H]-ouabain binding was studied in cerebral cortex membranes. It was observed that neurotensin modified binding in a dose-dependent manner, leading to 80% decrease with 1×10 4M concentration. On the other hand, the single addition of 1×10 -6M, 1×10 5M and 1×10 M SR 48692 (Sanofi-Aventis, U.S., Inc.) decreased [ 3H]-ouabain binding (in %) to 87±16; 74±16 and 34±17, respectively. Simultaneous addition of neurotensin and SR 48692 led to additive or synergic effects. Partial NTS2 agonist levocabastine inhibited [ 3H]-ouabain binding likewise. Saturation assays followed by Scatchard analyses showed that neurotensin increased K d value whereas failed to modify B max value, indicating a competitive type interaction of the peptide at Na +, K +-ATPase ouabain site. At variance, SR 48692 decreased B max value whereas it did not modify K d value. [ 3H]-ouabain binding was also studied in cerebral cortex membranes obtained from rats injected i. p. 30min earlier with 100μg and 250μg/kg SR 48692. It was observed that the 250μg/kg SR 48692 dose led to 19% decrease in basal [ 3H]-ouabain binding. After SR 48692 treatments, addition of 1×10 6M led to additive or synergic effect. Results suggested that [ 3H]-ouabain binding inhibition by neurotensin hardly involves NTS1 receptor. © 2010 Elsevier B.V.
Fil: Rosin, Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología. Cátedra de Farmacología; Argentina
Fil: López Ordieres, María Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología. Cátedra de Farmacología; Argentina
Fil: Rodriguez, Georgina Emma. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología. Cátedra de Farmacología; Argentina
description Previous work from this laboratory showed the ability of neurotensin to inhibit synaptosomal membrane Na +, K +-ATPase activity, the effect being blocked by SR 48692, a non-peptidic antagonist for high affinity neurotensin receptor (NTS1) [López Ordieres and Rodríguez de Lores Arnaiz 2000; 2001]. To further study neurotensin interaction with Na +, K +-ATPase, peptide effect on high affinity [ 3H]-ouabain binding was studied in cerebral cortex membranes. It was observed that neurotensin modified binding in a dose-dependent manner, leading to 80% decrease with 1×10 4M concentration. On the other hand, the single addition of 1×10 -6M, 1×10 5M and 1×10 M SR 48692 (Sanofi-Aventis, U.S., Inc.) decreased [ 3H]-ouabain binding (in %) to 87±16; 74±16 and 34±17, respectively. Simultaneous addition of neurotensin and SR 48692 led to additive or synergic effects. Partial NTS2 agonist levocabastine inhibited [ 3H]-ouabain binding likewise. Saturation assays followed by Scatchard analyses showed that neurotensin increased K d value whereas failed to modify B max value, indicating a competitive type interaction of the peptide at Na +, K +-ATPase ouabain site. At variance, SR 48692 decreased B max value whereas it did not modify K d value. [ 3H]-ouabain binding was also studied in cerebral cortex membranes obtained from rats injected i. p. 30min earlier with 100μg and 250μg/kg SR 48692. It was observed that the 250μg/kg SR 48692 dose led to 19% decrease in basal [ 3H]-ouabain binding. After SR 48692 treatments, addition of 1×10 6M led to additive or synergic effect. Results suggested that [ 3H]-ouabain binding inhibition by neurotensin hardly involves NTS1 receptor. © 2010 Elsevier B.V.
publishDate 2011
dc.date.none.fl_str_mv 2011-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/67552
Rosin, Carina; López Ordieres, María Graciela; Rodriguez, Georgina Emma; Neurotensin decreases high affinity [ 3H]-ouabain binding to cerebral cortex membranes; Elsevier Science; Regulatory Peptides; 172; 1-3; 12-2011; 35-40
0167-0115
CONICET Digital
CONICET
url http://hdl.handle.net/11336/67552
identifier_str_mv Rosin, Carina; López Ordieres, María Graciela; Rodriguez, Georgina Emma; Neurotensin decreases high affinity [ 3H]-ouabain binding to cerebral cortex membranes; Elsevier Science; Regulatory Peptides; 172; 1-3; 12-2011; 35-40
0167-0115
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.regpep.2011.08.004
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0167011511001522
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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