Neurotensin inhibits neuronal Na+, K+-ATPase activity through high affinity peptide receptor

Autores
López Ordieres, María Graciela; Rodriguez, Georgina Emma
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Neurotensin is a peptide present in mammalian CNS and peripheral tissues, which may play a major role in neurotransmission or neuromodulation, subserving diverse physiological functions. We studied the effect of added neurotensin on ATPase activities in synaptosomal membranes isolated from rat cerebral cortex. Neurotensin at 3 x 10-8-3 x 10-6 M concentration decreased 20-44% Na+, K+-ATPase activity but failed to modify Mg2+-ATPase activity; lower neurotensin concentrations (3 x 10-14-3 x 10-10 M) had no effect on enzyme activities. This inhibitory effect was abolished by neurotensin heating, by enzyme preincubation with neurotensin during periods exceeding 10 min, or by adding 1 x 10-6 M SR 48692, a high affinity neurotensin receptor antagonist. Levocabastine, which blocks low affinity neurotensin receptor, failed to alter enzyme inhibition by the peptide. It is suggested that the sodium pump may be a target for neurotensin effects at neuronal level involving the participation of high affinity neurotensin receptor. © 2000 Elsevier Science Inc.
Fil: López Ordieres, María Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia ; Argentina
Fil: Rodriguez, Georgina Emma. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia ; Argentina
Materia
Atpases
K+-Atpase
Na+
Neurotensin Effect
Neurotensin Receptor
Synaptosomal Membrane Enzymes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/39162
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/39162
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Neurotensin inhibits neuronal Na+, K+-ATPase activity through high affinity peptide receptorLópez Ordieres, María GracielaRodriguez, Georgina EmmaAtpasesK+-AtpaseNa+Neurotensin EffectNeurotensin ReceptorSynaptosomal Membrane Enzymeshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Neurotensin is a peptide present in mammalian CNS and peripheral tissues, which may play a major role in neurotransmission or neuromodulation, subserving diverse physiological functions. We studied the effect of added neurotensin on ATPase activities in synaptosomal membranes isolated from rat cerebral cortex. Neurotensin at 3 x 10-8-3 x 10-6 M concentration decreased 20-44% Na+, K+-ATPase activity but failed to modify Mg2+-ATPase activity; lower neurotensin concentrations (3 x 10-14-3 x 10-10 M) had no effect on enzyme activities. This inhibitory effect was abolished by neurotensin heating, by enzyme preincubation with neurotensin during periods exceeding 10 min, or by adding 1 x 10-6 M SR 48692, a high affinity neurotensin receptor antagonist. Levocabastine, which blocks low affinity neurotensin receptor, failed to alter enzyme inhibition by the peptide. It is suggested that the sodium pump may be a target for neurotensin effects at neuronal level involving the participation of high affinity neurotensin receptor. © 2000 Elsevier Science Inc.Fil: López Ordieres, María Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia ; ArgentinaFil: Rodriguez, Georgina Emma. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia ; ArgentinaElsevier Science Inc2000-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/39162López Ordieres, María Graciela; Rodriguez, Georgina Emma; Neurotensin inhibits neuronal Na+, K+-ATPase activity through high affinity peptide receptor; Elsevier Science Inc; Peptides; 21; 4; 3-2000; 571-5760196-9781CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://europepmc.org/abstract/med/10822114info:eu-repo/semantics/altIdentifier/doi/10.1016/S0196-9781(00)00183-2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:17Zoai:ri.conicet.gov.ar:11336/39162instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:17.787CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Neurotensin inhibits neuronal Na+, K+-ATPase activity through high affinity peptide receptor
title Neurotensin inhibits neuronal Na+, K+-ATPase activity through high affinity peptide receptor
spellingShingle Neurotensin inhibits neuronal Na+, K+-ATPase activity through high affinity peptide receptor
López Ordieres, María Graciela
Atpases
K+-Atpase
Na+
Neurotensin Effect
Neurotensin Receptor
Synaptosomal Membrane Enzymes
title_short Neurotensin inhibits neuronal Na+, K+-ATPase activity through high affinity peptide receptor
title_full Neurotensin inhibits neuronal Na+, K+-ATPase activity through high affinity peptide receptor
title_fullStr Neurotensin inhibits neuronal Na+, K+-ATPase activity through high affinity peptide receptor
title_full_unstemmed Neurotensin inhibits neuronal Na+, K+-ATPase activity through high affinity peptide receptor
title_sort Neurotensin inhibits neuronal Na+, K+-ATPase activity through high affinity peptide receptor
dc.creator.none.fl_str_mv López Ordieres, María Graciela
Rodriguez, Georgina Emma
author López Ordieres, María Graciela
author_facet López Ordieres, María Graciela
Rodriguez, Georgina Emma
author_role author
author2 Rodriguez, Georgina Emma
author2_role author
dc.subject.none.fl_str_mv Atpases
K+-Atpase
Na+
Neurotensin Effect
Neurotensin Receptor
Synaptosomal Membrane Enzymes
topic Atpases
K+-Atpase
Na+
Neurotensin Effect
Neurotensin Receptor
Synaptosomal Membrane Enzymes
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Neurotensin is a peptide present in mammalian CNS and peripheral tissues, which may play a major role in neurotransmission or neuromodulation, subserving diverse physiological functions. We studied the effect of added neurotensin on ATPase activities in synaptosomal membranes isolated from rat cerebral cortex. Neurotensin at 3 x 10-8-3 x 10-6 M concentration decreased 20-44% Na+, K+-ATPase activity but failed to modify Mg2+-ATPase activity; lower neurotensin concentrations (3 x 10-14-3 x 10-10 M) had no effect on enzyme activities. This inhibitory effect was abolished by neurotensin heating, by enzyme preincubation with neurotensin during periods exceeding 10 min, or by adding 1 x 10-6 M SR 48692, a high affinity neurotensin receptor antagonist. Levocabastine, which blocks low affinity neurotensin receptor, failed to alter enzyme inhibition by the peptide. It is suggested that the sodium pump may be a target for neurotensin effects at neuronal level involving the participation of high affinity neurotensin receptor. © 2000 Elsevier Science Inc.
Fil: López Ordieres, María Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia ; Argentina
Fil: Rodriguez, Georgina Emma. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia ; Argentina
description Neurotensin is a peptide present in mammalian CNS and peripheral tissues, which may play a major role in neurotransmission or neuromodulation, subserving diverse physiological functions. We studied the effect of added neurotensin on ATPase activities in synaptosomal membranes isolated from rat cerebral cortex. Neurotensin at 3 x 10-8-3 x 10-6 M concentration decreased 20-44% Na+, K+-ATPase activity but failed to modify Mg2+-ATPase activity; lower neurotensin concentrations (3 x 10-14-3 x 10-10 M) had no effect on enzyme activities. This inhibitory effect was abolished by neurotensin heating, by enzyme preincubation with neurotensin during periods exceeding 10 min, or by adding 1 x 10-6 M SR 48692, a high affinity neurotensin receptor antagonist. Levocabastine, which blocks low affinity neurotensin receptor, failed to alter enzyme inhibition by the peptide. It is suggested that the sodium pump may be a target for neurotensin effects at neuronal level involving the participation of high affinity neurotensin receptor. © 2000 Elsevier Science Inc.
publishDate 2000
dc.date.none.fl_str_mv 2000-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/39162
López Ordieres, María Graciela; Rodriguez, Georgina Emma; Neurotensin inhibits neuronal Na+, K+-ATPase activity through high affinity peptide receptor; Elsevier Science Inc; Peptides; 21; 4; 3-2000; 571-576
0196-9781
CONICET Digital
CONICET
url http://hdl.handle.net/11336/39162
identifier_str_mv López Ordieres, María Graciela; Rodriguez, Georgina Emma; Neurotensin inhibits neuronal Na+, K+-ATPase activity through high affinity peptide receptor; Elsevier Science Inc; Peptides; 21; 4; 3-2000; 571-576
0196-9781
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://europepmc.org/abstract/med/10822114
info:eu-repo/semantics/altIdentifier/doi/10.1016/S0196-9781(00)00183-2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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