Genomic analysis of Acinetobacter baumannii A118 by comparison of optical maps: Identification of structures related to its susceptibility phenotype

Autores
Ramirez, Maria Soledad; Adams, Mark D.; Bonomo, Robert A.; Centron, Daniela; Tolmasky, Marcelo E.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Acinetobacter baumannii A118, a naturally competent clinical isolate, is unusually susceptible to several antibiotics. Comparison of the optical map of strain A118 with in silico-generated restriction maps of sequenced genomes and sequence analyses showed that the AbaR region, commonly found inserted within the comM gene in other isolates, is missing in strain A118, which could in part explain the susceptible phenotype exhibited by this isolate. These comparative studies also showed differences in regions where genes coding for functions that may be involved in drug resistance or susceptibility are located. Further sequencing demonstrated that cat and blaADC, named blaADC-55, are present but that a tet(A) gene usually found in other strains is not. In addition, carO and pbp2, which may play a role in susceptibility to carbapenems, are present in strain A118. These findings support the idea that A. baumannii strains possess multiple mechanisms that contribute to antibiotic resistance, and the presence of some of them is not sufficient for a resistant phenotype. The results shown here indicate that optical mapping is a useful tool for preliminary comparative genomic analysis.
Fil: Ramirez, Maria Soledad. Universidad de Buenos Aires. Facultad de Medicina; Argentina. California State University Fullerton; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Adams, Mark D.. Case Western Reserve University; Estados Unidos
Fil: Bonomo, Robert A.. Case Western Reserve University; Estados Unidos
Fil: Centron, Daniela. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tolmasky, Marcelo E.. California State University Fullerton; Estados Unidos
Materia
ACINETOBACTER BAUMANNII
OPTICAL MAPPING
NOSOCOMIAL INFECTION
ANTIBIOTIC RESISTANCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/67451
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Genomic analysis of Acinetobacter baumannii A118 by comparison of optical maps: Identification of structures related to its susceptibility phenotypeRamirez, Maria SoledadAdams, Mark D.Bonomo, Robert A.Centron, DanielaTolmasky, Marcelo E.ACINETOBACTER BAUMANNIIOPTICAL MAPPINGNOSOCOMIAL INFECTIONANTIBIOTIC RESISTANCEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Acinetobacter baumannii A118, a naturally competent clinical isolate, is unusually susceptible to several antibiotics. Comparison of the optical map of strain A118 with in silico-generated restriction maps of sequenced genomes and sequence analyses showed that the AbaR region, commonly found inserted within the comM gene in other isolates, is missing in strain A118, which could in part explain the susceptible phenotype exhibited by this isolate. These comparative studies also showed differences in regions where genes coding for functions that may be involved in drug resistance or susceptibility are located. Further sequencing demonstrated that cat and blaADC, named blaADC-55, are present but that a tet(A) gene usually found in other strains is not. In addition, carO and pbp2, which may play a role in susceptibility to carbapenems, are present in strain A118. These findings support the idea that A. baumannii strains possess multiple mechanisms that contribute to antibiotic resistance, and the presence of some of them is not sufficient for a resistant phenotype. The results shown here indicate that optical mapping is a useful tool for preliminary comparative genomic analysis.Fil: Ramirez, Maria Soledad. Universidad de Buenos Aires. Facultad de Medicina; Argentina. California State University Fullerton; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Adams, Mark D.. Case Western Reserve University; Estados UnidosFil: Bonomo, Robert A.. Case Western Reserve University; Estados UnidosFil: Centron, Daniela. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tolmasky, Marcelo E.. California State University Fullerton; Estados UnidosAmerican Society for Microbiology2011-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/67451Ramirez, Maria Soledad; Adams, Mark D.; Bonomo, Robert A.; Centron, Daniela; Tolmasky, Marcelo E.; Genomic analysis of Acinetobacter baumannii A118 by comparison of optical maps: Identification of structures related to its susceptibility phenotype; American Society for Microbiology; Antimicrobial Agents and Chemotherapy; 55; 4; 4-2011; 1520-15260066-4804CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1128/AAC.01595-10info:eu-repo/semantics/altIdentifier/url/https://aac.asm.org/content/55/4/1520info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:28:46Zoai:ri.conicet.gov.ar:11336/67451instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:28:46.882CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genomic analysis of Acinetobacter baumannii A118 by comparison of optical maps: Identification of structures related to its susceptibility phenotype
title Genomic analysis of Acinetobacter baumannii A118 by comparison of optical maps: Identification of structures related to its susceptibility phenotype
spellingShingle Genomic analysis of Acinetobacter baumannii A118 by comparison of optical maps: Identification of structures related to its susceptibility phenotype
Ramirez, Maria Soledad
ACINETOBACTER BAUMANNII
OPTICAL MAPPING
NOSOCOMIAL INFECTION
ANTIBIOTIC RESISTANCE
title_short Genomic analysis of Acinetobacter baumannii A118 by comparison of optical maps: Identification of structures related to its susceptibility phenotype
title_full Genomic analysis of Acinetobacter baumannii A118 by comparison of optical maps: Identification of structures related to its susceptibility phenotype
title_fullStr Genomic analysis of Acinetobacter baumannii A118 by comparison of optical maps: Identification of structures related to its susceptibility phenotype
title_full_unstemmed Genomic analysis of Acinetobacter baumannii A118 by comparison of optical maps: Identification of structures related to its susceptibility phenotype
title_sort Genomic analysis of Acinetobacter baumannii A118 by comparison of optical maps: Identification of structures related to its susceptibility phenotype
dc.creator.none.fl_str_mv Ramirez, Maria Soledad
Adams, Mark D.
Bonomo, Robert A.
Centron, Daniela
Tolmasky, Marcelo E.
author Ramirez, Maria Soledad
author_facet Ramirez, Maria Soledad
Adams, Mark D.
Bonomo, Robert A.
Centron, Daniela
Tolmasky, Marcelo E.
author_role author
author2 Adams, Mark D.
Bonomo, Robert A.
Centron, Daniela
Tolmasky, Marcelo E.
author2_role author
author
author
author
dc.subject.none.fl_str_mv ACINETOBACTER BAUMANNII
OPTICAL MAPPING
NOSOCOMIAL INFECTION
ANTIBIOTIC RESISTANCE
topic ACINETOBACTER BAUMANNII
OPTICAL MAPPING
NOSOCOMIAL INFECTION
ANTIBIOTIC RESISTANCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Acinetobacter baumannii A118, a naturally competent clinical isolate, is unusually susceptible to several antibiotics. Comparison of the optical map of strain A118 with in silico-generated restriction maps of sequenced genomes and sequence analyses showed that the AbaR region, commonly found inserted within the comM gene in other isolates, is missing in strain A118, which could in part explain the susceptible phenotype exhibited by this isolate. These comparative studies also showed differences in regions where genes coding for functions that may be involved in drug resistance or susceptibility are located. Further sequencing demonstrated that cat and blaADC, named blaADC-55, are present but that a tet(A) gene usually found in other strains is not. In addition, carO and pbp2, which may play a role in susceptibility to carbapenems, are present in strain A118. These findings support the idea that A. baumannii strains possess multiple mechanisms that contribute to antibiotic resistance, and the presence of some of them is not sufficient for a resistant phenotype. The results shown here indicate that optical mapping is a useful tool for preliminary comparative genomic analysis.
Fil: Ramirez, Maria Soledad. Universidad de Buenos Aires. Facultad de Medicina; Argentina. California State University Fullerton; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Adams, Mark D.. Case Western Reserve University; Estados Unidos
Fil: Bonomo, Robert A.. Case Western Reserve University; Estados Unidos
Fil: Centron, Daniela. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tolmasky, Marcelo E.. California State University Fullerton; Estados Unidos
description Acinetobacter baumannii A118, a naturally competent clinical isolate, is unusually susceptible to several antibiotics. Comparison of the optical map of strain A118 with in silico-generated restriction maps of sequenced genomes and sequence analyses showed that the AbaR region, commonly found inserted within the comM gene in other isolates, is missing in strain A118, which could in part explain the susceptible phenotype exhibited by this isolate. These comparative studies also showed differences in regions where genes coding for functions that may be involved in drug resistance or susceptibility are located. Further sequencing demonstrated that cat and blaADC, named blaADC-55, are present but that a tet(A) gene usually found in other strains is not. In addition, carO and pbp2, which may play a role in susceptibility to carbapenems, are present in strain A118. These findings support the idea that A. baumannii strains possess multiple mechanisms that contribute to antibiotic resistance, and the presence of some of them is not sufficient for a resistant phenotype. The results shown here indicate that optical mapping is a useful tool for preliminary comparative genomic analysis.
publishDate 2011
dc.date.none.fl_str_mv 2011-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/67451
Ramirez, Maria Soledad; Adams, Mark D.; Bonomo, Robert A.; Centron, Daniela; Tolmasky, Marcelo E.; Genomic analysis of Acinetobacter baumannii A118 by comparison of optical maps: Identification of structures related to its susceptibility phenotype; American Society for Microbiology; Antimicrobial Agents and Chemotherapy; 55; 4; 4-2011; 1520-1526
0066-4804
CONICET Digital
CONICET
url http://hdl.handle.net/11336/67451
identifier_str_mv Ramirez, Maria Soledad; Adams, Mark D.; Bonomo, Robert A.; Centron, Daniela; Tolmasky, Marcelo E.; Genomic analysis of Acinetobacter baumannii A118 by comparison of optical maps: Identification of structures related to its susceptibility phenotype; American Society for Microbiology; Antimicrobial Agents and Chemotherapy; 55; 4; 4-2011; 1520-1526
0066-4804
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1128/AAC.01595-10
info:eu-repo/semantics/altIdentifier/url/https://aac.asm.org/content/55/4/1520
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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