Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the Host Determine the Outcome?
- Autores
- Pimentel, Camila; Le, Casin; Tuttobene, Marisel Romina; Subils, Tomás; Papp Wallace, Krisztina M.; Bonomo, Robert A.; Tolmasky, Marcelo E.; Ramirez, Maria Soledad
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Acinetobacter baumannii has become a serious threat to human health due to its extreme antibiotic resistance, environmental persistence, and capacity to survive within the host. Two A. baumannii strains, A118 and AB5075, commonly used as model systems, and three carbapenem-resistant strains, which are becoming ever more dangerous due to the multiple drugs they can resist, were exposed to 3.5% human serum albumin (HSA) and human serum (HS) to evaluate their response with respect to antimicrobial resistance, biofilm formation, and quorum sensing, all features responsible for increasing survival and persistence in the environment and human body. Expression levels of antibiotic resistance genes were modified differently when examined in different strains. The cmlA gene was upregulated or downregulated in conditions of exposure to 3.5% HSA or HS depending on the strain. Expression levels of pbp1 and pbp3 tended to be increased by the presence of HSA and HS, but the effect was not seen in all strains. A. baumannii A118 growing in the presence of HS did not experience increased expression of these genes. Aminoglycoside-modifying enzymes were also expressed at higher or lower levels in the presence of HSA or HS. Still, the response was not uniform; in some cases, expression was enhanced, and in other cases, it was tapered. While A. baumannii AB5075 became more susceptible to rifampicin in the presence of 3.5% HSA or HS, strain A118 did not show any changes. Expression of arr2, a gene involved in resistance to rifampicin present in A. baumannii AMA16, was expressed at higher levels when HS was present in the culture medium. HSA and HS reduced biofilm formation and production of N-Acyl Homoserine Lactone, a compound intimately associated with quorum sensing. In conclusion, HSA, the main component of HS, stimulates a variety of adaptative responses in infecting A. baumannii strains.
Fil: Pimentel, Camila. University of California; Estados Unidos
Fil: Le, Casin. University of California; Estados Unidos
Fil: Tuttobene, Marisel Romina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Subils, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Papp Wallace, Krisztina M.. Case Western Reserve University School of Medicine; Estados Unidos
Fil: Bonomo, Robert A.. Case Western Reserve University School of Medicine; Estados Unidos
Fil: Tolmasky, Marcelo E.. University of California; Estados Unidos
Fil: Ramirez, Maria Soledad. University of California; Estados Unidos - Materia
-
ACINETOBACTER BAUMANNII
ALBUMIN
ANTIBIOTIC RESISTANCE
BIOFILM
HUMAN SERUM
QUORUM SENSING - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/182588
Ver los metadatos del registro completo
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Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the Host Determine the Outcome?Pimentel, CamilaLe, CasinTuttobene, Marisel RominaSubils, TomásPapp Wallace, Krisztina M.Bonomo, Robert A.Tolmasky, Marcelo E.Ramirez, Maria SoledadACINETOBACTER BAUMANNIIALBUMINANTIBIOTIC RESISTANCEBIOFILMHUMAN SERUMQUORUM SENSINGhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Acinetobacter baumannii has become a serious threat to human health due to its extreme antibiotic resistance, environmental persistence, and capacity to survive within the host. Two A. baumannii strains, A118 and AB5075, commonly used as model systems, and three carbapenem-resistant strains, which are becoming ever more dangerous due to the multiple drugs they can resist, were exposed to 3.5% human serum albumin (HSA) and human serum (HS) to evaluate their response with respect to antimicrobial resistance, biofilm formation, and quorum sensing, all features responsible for increasing survival and persistence in the environment and human body. Expression levels of antibiotic resistance genes were modified differently when examined in different strains. The cmlA gene was upregulated or downregulated in conditions of exposure to 3.5% HSA or HS depending on the strain. Expression levels of pbp1 and pbp3 tended to be increased by the presence of HSA and HS, but the effect was not seen in all strains. A. baumannii A118 growing in the presence of HS did not experience increased expression of these genes. Aminoglycoside-modifying enzymes were also expressed at higher or lower levels in the presence of HSA or HS. Still, the response was not uniform; in some cases, expression was enhanced, and in other cases, it was tapered. While A. baumannii AB5075 became more susceptible to rifampicin in the presence of 3.5% HSA or HS, strain A118 did not show any changes. Expression of arr2, a gene involved in resistance to rifampicin present in A. baumannii AMA16, was expressed at higher levels when HS was present in the culture medium. HSA and HS reduced biofilm formation and production of N-Acyl Homoserine Lactone, a compound intimately associated with quorum sensing. In conclusion, HSA, the main component of HS, stimulates a variety of adaptative responses in infecting A. baumannii strains.Fil: Pimentel, Camila. University of California; Estados UnidosFil: Le, Casin. University of California; Estados UnidosFil: Tuttobene, Marisel Romina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Subils, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; ArgentinaFil: Papp Wallace, Krisztina M.. Case Western Reserve University School of Medicine; Estados UnidosFil: Bonomo, Robert A.. Case Western Reserve University School of Medicine; Estados UnidosFil: Tolmasky, Marcelo E.. University of California; Estados UnidosFil: Ramirez, Maria Soledad. University of California; Estados UnidosMDPI AG2021-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/182588Pimentel, Camila; Le, Casin; Tuttobene, Marisel Romina; Subils, Tomás; Papp Wallace, Krisztina M.; et al.; Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the Host Determine the Outcome?; MDPI AG; Antibiotics; 10; 7; 7-2021; 1-142079-6382CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2079-6382/10/7/833info:eu-repo/semantics/altIdentifier/doi/10.3390/antibiotics10070833info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:57Zoai:ri.conicet.gov.ar:11336/182588instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:58.201CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the Host Determine the Outcome? |
title |
Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the Host Determine the Outcome? |
spellingShingle |
Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the Host Determine the Outcome? Pimentel, Camila ACINETOBACTER BAUMANNII ALBUMIN ANTIBIOTIC RESISTANCE BIOFILM HUMAN SERUM QUORUM SENSING |
title_short |
Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the Host Determine the Outcome? |
title_full |
Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the Host Determine the Outcome? |
title_fullStr |
Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the Host Determine the Outcome? |
title_full_unstemmed |
Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the Host Determine the Outcome? |
title_sort |
Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the Host Determine the Outcome? |
dc.creator.none.fl_str_mv |
Pimentel, Camila Le, Casin Tuttobene, Marisel Romina Subils, Tomás Papp Wallace, Krisztina M. Bonomo, Robert A. Tolmasky, Marcelo E. Ramirez, Maria Soledad |
author |
Pimentel, Camila |
author_facet |
Pimentel, Camila Le, Casin Tuttobene, Marisel Romina Subils, Tomás Papp Wallace, Krisztina M. Bonomo, Robert A. Tolmasky, Marcelo E. Ramirez, Maria Soledad |
author_role |
author |
author2 |
Le, Casin Tuttobene, Marisel Romina Subils, Tomás Papp Wallace, Krisztina M. Bonomo, Robert A. Tolmasky, Marcelo E. Ramirez, Maria Soledad |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
ACINETOBACTER BAUMANNII ALBUMIN ANTIBIOTIC RESISTANCE BIOFILM HUMAN SERUM QUORUM SENSING |
topic |
ACINETOBACTER BAUMANNII ALBUMIN ANTIBIOTIC RESISTANCE BIOFILM HUMAN SERUM QUORUM SENSING |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Acinetobacter baumannii has become a serious threat to human health due to its extreme antibiotic resistance, environmental persistence, and capacity to survive within the host. Two A. baumannii strains, A118 and AB5075, commonly used as model systems, and three carbapenem-resistant strains, which are becoming ever more dangerous due to the multiple drugs they can resist, were exposed to 3.5% human serum albumin (HSA) and human serum (HS) to evaluate their response with respect to antimicrobial resistance, biofilm formation, and quorum sensing, all features responsible for increasing survival and persistence in the environment and human body. Expression levels of antibiotic resistance genes were modified differently when examined in different strains. The cmlA gene was upregulated or downregulated in conditions of exposure to 3.5% HSA or HS depending on the strain. Expression levels of pbp1 and pbp3 tended to be increased by the presence of HSA and HS, but the effect was not seen in all strains. A. baumannii A118 growing in the presence of HS did not experience increased expression of these genes. Aminoglycoside-modifying enzymes were also expressed at higher or lower levels in the presence of HSA or HS. Still, the response was not uniform; in some cases, expression was enhanced, and in other cases, it was tapered. While A. baumannii AB5075 became more susceptible to rifampicin in the presence of 3.5% HSA or HS, strain A118 did not show any changes. Expression of arr2, a gene involved in resistance to rifampicin present in A. baumannii AMA16, was expressed at higher levels when HS was present in the culture medium. HSA and HS reduced biofilm formation and production of N-Acyl Homoserine Lactone, a compound intimately associated with quorum sensing. In conclusion, HSA, the main component of HS, stimulates a variety of adaptative responses in infecting A. baumannii strains. Fil: Pimentel, Camila. University of California; Estados Unidos Fil: Le, Casin. University of California; Estados Unidos Fil: Tuttobene, Marisel Romina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina Fil: Subils, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina Fil: Papp Wallace, Krisztina M.. Case Western Reserve University School of Medicine; Estados Unidos Fil: Bonomo, Robert A.. Case Western Reserve University School of Medicine; Estados Unidos Fil: Tolmasky, Marcelo E.. University of California; Estados Unidos Fil: Ramirez, Maria Soledad. University of California; Estados Unidos |
description |
Acinetobacter baumannii has become a serious threat to human health due to its extreme antibiotic resistance, environmental persistence, and capacity to survive within the host. Two A. baumannii strains, A118 and AB5075, commonly used as model systems, and three carbapenem-resistant strains, which are becoming ever more dangerous due to the multiple drugs they can resist, were exposed to 3.5% human serum albumin (HSA) and human serum (HS) to evaluate their response with respect to antimicrobial resistance, biofilm formation, and quorum sensing, all features responsible for increasing survival and persistence in the environment and human body. Expression levels of antibiotic resistance genes were modified differently when examined in different strains. The cmlA gene was upregulated or downregulated in conditions of exposure to 3.5% HSA or HS depending on the strain. Expression levels of pbp1 and pbp3 tended to be increased by the presence of HSA and HS, but the effect was not seen in all strains. A. baumannii A118 growing in the presence of HS did not experience increased expression of these genes. Aminoglycoside-modifying enzymes were also expressed at higher or lower levels in the presence of HSA or HS. Still, the response was not uniform; in some cases, expression was enhanced, and in other cases, it was tapered. While A. baumannii AB5075 became more susceptible to rifampicin in the presence of 3.5% HSA or HS, strain A118 did not show any changes. Expression of arr2, a gene involved in resistance to rifampicin present in A. baumannii AMA16, was expressed at higher levels when HS was present in the culture medium. HSA and HS reduced biofilm formation and production of N-Acyl Homoserine Lactone, a compound intimately associated with quorum sensing. In conclusion, HSA, the main component of HS, stimulates a variety of adaptative responses in infecting A. baumannii strains. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/182588 Pimentel, Camila; Le, Casin; Tuttobene, Marisel Romina; Subils, Tomás; Papp Wallace, Krisztina M.; et al.; Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the Host Determine the Outcome?; MDPI AG; Antibiotics; 10; 7; 7-2021; 1-14 2079-6382 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/182588 |
identifier_str_mv |
Pimentel, Camila; Le, Casin; Tuttobene, Marisel Romina; Subils, Tomás; Papp Wallace, Krisztina M.; et al.; Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the Host Determine the Outcome?; MDPI AG; Antibiotics; 10; 7; 7-2021; 1-14 2079-6382 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2079-6382/10/7/833 info:eu-repo/semantics/altIdentifier/doi/10.3390/antibiotics10070833 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
MDPI AG |
publisher.none.fl_str_mv |
MDPI AG |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.13397 |