Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the Host Determine the Outcome?

Autores
Pimentel, Camila; Le, Casin; Tuttobene, Marisel Romina; Subils, Tomás; Papp Wallace, Krisztina M.; Bonomo, Robert A.; Tolmasky, Marcelo E.; Ramirez, Maria Soledad
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Acinetobacter baumannii has become a serious threat to human health due to its extreme antibiotic resistance, environmental persistence, and capacity to survive within the host. Two A. baumannii strains, A118 and AB5075, commonly used as model systems, and three carbapenem-resistant strains, which are becoming ever more dangerous due to the multiple drugs they can resist, were exposed to 3.5% human serum albumin (HSA) and human serum (HS) to evaluate their response with respect to antimicrobial resistance, biofilm formation, and quorum sensing, all features responsible for increasing survival and persistence in the environment and human body. Expression levels of antibiotic resistance genes were modified differently when examined in different strains. The cmlA gene was upregulated or downregulated in conditions of exposure to 3.5% HSA or HS depending on the strain. Expression levels of pbp1 and pbp3 tended to be increased by the presence of HSA and HS, but the effect was not seen in all strains. A. baumannii A118 growing in the presence of HS did not experience increased expression of these genes. Aminoglycoside-modifying enzymes were also expressed at higher or lower levels in the presence of HSA or HS. Still, the response was not uniform; in some cases, expression was enhanced, and in other cases, it was tapered. While A. baumannii AB5075 became more susceptible to rifampicin in the presence of 3.5% HSA or HS, strain A118 did not show any changes. Expression of arr2, a gene involved in resistance to rifampicin present in A. baumannii AMA16, was expressed at higher levels when HS was present in the culture medium. HSA and HS reduced biofilm formation and production of N-Acyl Homoserine Lactone, a compound intimately associated with quorum sensing. In conclusion, HSA, the main component of HS, stimulates a variety of adaptative responses in infecting A. baumannii strains.
Fil: Pimentel, Camila. University of California; Estados Unidos
Fil: Le, Casin. University of California; Estados Unidos
Fil: Tuttobene, Marisel Romina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Subils, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Papp Wallace, Krisztina M.. Case Western Reserve University School of Medicine; Estados Unidos
Fil: Bonomo, Robert A.. Case Western Reserve University School of Medicine; Estados Unidos
Fil: Tolmasky, Marcelo E.. University of California; Estados Unidos
Fil: Ramirez, Maria Soledad. University of California; Estados Unidos
Materia
ACINETOBACTER BAUMANNII
ALBUMIN
ANTIBIOTIC RESISTANCE
BIOFILM
HUMAN SERUM
QUORUM SENSING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/182588

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network_name_str CONICET Digital (CONICET)
spelling Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the Host Determine the Outcome?Pimentel, CamilaLe, CasinTuttobene, Marisel RominaSubils, TomásPapp Wallace, Krisztina M.Bonomo, Robert A.Tolmasky, Marcelo E.Ramirez, Maria SoledadACINETOBACTER BAUMANNIIALBUMINANTIBIOTIC RESISTANCEBIOFILMHUMAN SERUMQUORUM SENSINGhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Acinetobacter baumannii has become a serious threat to human health due to its extreme antibiotic resistance, environmental persistence, and capacity to survive within the host. Two A. baumannii strains, A118 and AB5075, commonly used as model systems, and three carbapenem-resistant strains, which are becoming ever more dangerous due to the multiple drugs they can resist, were exposed to 3.5% human serum albumin (HSA) and human serum (HS) to evaluate their response with respect to antimicrobial resistance, biofilm formation, and quorum sensing, all features responsible for increasing survival and persistence in the environment and human body. Expression levels of antibiotic resistance genes were modified differently when examined in different strains. The cmlA gene was upregulated or downregulated in conditions of exposure to 3.5% HSA or HS depending on the strain. Expression levels of pbp1 and pbp3 tended to be increased by the presence of HSA and HS, but the effect was not seen in all strains. A. baumannii A118 growing in the presence of HS did not experience increased expression of these genes. Aminoglycoside-modifying enzymes were also expressed at higher or lower levels in the presence of HSA or HS. Still, the response was not uniform; in some cases, expression was enhanced, and in other cases, it was tapered. While A. baumannii AB5075 became more susceptible to rifampicin in the presence of 3.5% HSA or HS, strain A118 did not show any changes. Expression of arr2, a gene involved in resistance to rifampicin present in A. baumannii AMA16, was expressed at higher levels when HS was present in the culture medium. HSA and HS reduced biofilm formation and production of N-Acyl Homoserine Lactone, a compound intimately associated with quorum sensing. In conclusion, HSA, the main component of HS, stimulates a variety of adaptative responses in infecting A. baumannii strains.Fil: Pimentel, Camila. University of California; Estados UnidosFil: Le, Casin. University of California; Estados UnidosFil: Tuttobene, Marisel Romina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Subils, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; ArgentinaFil: Papp Wallace, Krisztina M.. Case Western Reserve University School of Medicine; Estados UnidosFil: Bonomo, Robert A.. Case Western Reserve University School of Medicine; Estados UnidosFil: Tolmasky, Marcelo E.. University of California; Estados UnidosFil: Ramirez, Maria Soledad. University of California; Estados UnidosMDPI AG2021-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/182588Pimentel, Camila; Le, Casin; Tuttobene, Marisel Romina; Subils, Tomás; Papp Wallace, Krisztina M.; et al.; Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the Host Determine the Outcome?; MDPI AG; Antibiotics; 10; 7; 7-2021; 1-142079-6382CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2079-6382/10/7/833info:eu-repo/semantics/altIdentifier/doi/10.3390/antibiotics10070833info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:57Zoai:ri.conicet.gov.ar:11336/182588instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:58.201CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the Host Determine the Outcome?
title Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the Host Determine the Outcome?
spellingShingle Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the Host Determine the Outcome?
Pimentel, Camila
ACINETOBACTER BAUMANNII
ALBUMIN
ANTIBIOTIC RESISTANCE
BIOFILM
HUMAN SERUM
QUORUM SENSING
title_short Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the Host Determine the Outcome?
title_full Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the Host Determine the Outcome?
title_fullStr Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the Host Determine the Outcome?
title_full_unstemmed Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the Host Determine the Outcome?
title_sort Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the Host Determine the Outcome?
dc.creator.none.fl_str_mv Pimentel, Camila
Le, Casin
Tuttobene, Marisel Romina
Subils, Tomás
Papp Wallace, Krisztina M.
Bonomo, Robert A.
Tolmasky, Marcelo E.
Ramirez, Maria Soledad
author Pimentel, Camila
author_facet Pimentel, Camila
Le, Casin
Tuttobene, Marisel Romina
Subils, Tomás
Papp Wallace, Krisztina M.
Bonomo, Robert A.
Tolmasky, Marcelo E.
Ramirez, Maria Soledad
author_role author
author2 Le, Casin
Tuttobene, Marisel Romina
Subils, Tomás
Papp Wallace, Krisztina M.
Bonomo, Robert A.
Tolmasky, Marcelo E.
Ramirez, Maria Soledad
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ACINETOBACTER BAUMANNII
ALBUMIN
ANTIBIOTIC RESISTANCE
BIOFILM
HUMAN SERUM
QUORUM SENSING
topic ACINETOBACTER BAUMANNII
ALBUMIN
ANTIBIOTIC RESISTANCE
BIOFILM
HUMAN SERUM
QUORUM SENSING
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Acinetobacter baumannii has become a serious threat to human health due to its extreme antibiotic resistance, environmental persistence, and capacity to survive within the host. Two A. baumannii strains, A118 and AB5075, commonly used as model systems, and three carbapenem-resistant strains, which are becoming ever more dangerous due to the multiple drugs they can resist, were exposed to 3.5% human serum albumin (HSA) and human serum (HS) to evaluate their response with respect to antimicrobial resistance, biofilm formation, and quorum sensing, all features responsible for increasing survival and persistence in the environment and human body. Expression levels of antibiotic resistance genes were modified differently when examined in different strains. The cmlA gene was upregulated or downregulated in conditions of exposure to 3.5% HSA or HS depending on the strain. Expression levels of pbp1 and pbp3 tended to be increased by the presence of HSA and HS, but the effect was not seen in all strains. A. baumannii A118 growing in the presence of HS did not experience increased expression of these genes. Aminoglycoside-modifying enzymes were also expressed at higher or lower levels in the presence of HSA or HS. Still, the response was not uniform; in some cases, expression was enhanced, and in other cases, it was tapered. While A. baumannii AB5075 became more susceptible to rifampicin in the presence of 3.5% HSA or HS, strain A118 did not show any changes. Expression of arr2, a gene involved in resistance to rifampicin present in A. baumannii AMA16, was expressed at higher levels when HS was present in the culture medium. HSA and HS reduced biofilm formation and production of N-Acyl Homoserine Lactone, a compound intimately associated with quorum sensing. In conclusion, HSA, the main component of HS, stimulates a variety of adaptative responses in infecting A. baumannii strains.
Fil: Pimentel, Camila. University of California; Estados Unidos
Fil: Le, Casin. University of California; Estados Unidos
Fil: Tuttobene, Marisel Romina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Subils, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Papp Wallace, Krisztina M.. Case Western Reserve University School of Medicine; Estados Unidos
Fil: Bonomo, Robert A.. Case Western Reserve University School of Medicine; Estados Unidos
Fil: Tolmasky, Marcelo E.. University of California; Estados Unidos
Fil: Ramirez, Maria Soledad. University of California; Estados Unidos
description Acinetobacter baumannii has become a serious threat to human health due to its extreme antibiotic resistance, environmental persistence, and capacity to survive within the host. Two A. baumannii strains, A118 and AB5075, commonly used as model systems, and three carbapenem-resistant strains, which are becoming ever more dangerous due to the multiple drugs they can resist, were exposed to 3.5% human serum albumin (HSA) and human serum (HS) to evaluate their response with respect to antimicrobial resistance, biofilm formation, and quorum sensing, all features responsible for increasing survival and persistence in the environment and human body. Expression levels of antibiotic resistance genes were modified differently when examined in different strains. The cmlA gene was upregulated or downregulated in conditions of exposure to 3.5% HSA or HS depending on the strain. Expression levels of pbp1 and pbp3 tended to be increased by the presence of HSA and HS, but the effect was not seen in all strains. A. baumannii A118 growing in the presence of HS did not experience increased expression of these genes. Aminoglycoside-modifying enzymes were also expressed at higher or lower levels in the presence of HSA or HS. Still, the response was not uniform; in some cases, expression was enhanced, and in other cases, it was tapered. While A. baumannii AB5075 became more susceptible to rifampicin in the presence of 3.5% HSA or HS, strain A118 did not show any changes. Expression of arr2, a gene involved in resistance to rifampicin present in A. baumannii AMA16, was expressed at higher levels when HS was present in the culture medium. HSA and HS reduced biofilm formation and production of N-Acyl Homoserine Lactone, a compound intimately associated with quorum sensing. In conclusion, HSA, the main component of HS, stimulates a variety of adaptative responses in infecting A. baumannii strains.
publishDate 2021
dc.date.none.fl_str_mv 2021-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/182588
Pimentel, Camila; Le, Casin; Tuttobene, Marisel Romina; Subils, Tomás; Papp Wallace, Krisztina M.; et al.; Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the Host Determine the Outcome?; MDPI AG; Antibiotics; 10; 7; 7-2021; 1-14
2079-6382
CONICET Digital
CONICET
url http://hdl.handle.net/11336/182588
identifier_str_mv Pimentel, Camila; Le, Casin; Tuttobene, Marisel Romina; Subils, Tomás; Papp Wallace, Krisztina M.; et al.; Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the Host Determine the Outcome?; MDPI AG; Antibiotics; 10; 7; 7-2021; 1-14
2079-6382
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2079-6382/10/7/833
info:eu-repo/semantics/altIdentifier/doi/10.3390/antibiotics10070833
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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