Circadian rhythms in the critical human pathogen Acinetobacter baumannii

Autores
Mussi, María Alejandra; Permingeat, Valentin; Perez Mora, Barbara Edith; Migliori, María Laura; Arana, Natalia Alejandra; Fernández, Julia; Allasia, María Belén; Lamberti, Melisa Luciana; Di Venanzio, Gisela Andrea; Feldman, Mario; Golombek, Diego Andrés
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Acinetobacter baumannii is recognized as the paradigm of multidrug resistant superbug, topping the WHO priority list of critical human pathogens. Interestingly, it senses and responds to blue light, which modulates global aspects of its physiology including the pathogenicity. We hypothesized that light could serve as a signal to synchronize the bacterial physiology to the host’s behavior, or to the environment. At environmental temperatures, light regulation is mainly governed by the BLUF-type photoreceptor BlsA. In this work, we identified the existence of daily rhythms in blsA expression displaying a robust response to light, as well as endogenous circadian rhythms in A. baumannii. In fact, we show that blsA gene expression can be synchronized to 24-hour blue light-dark cycles, which immediately resynchronizes after a phase shift due to a longer night. Upon release to constant darkness, bacterial populations present free-running oscillations with a period close to 24 hours. Furthermore, our data indicate that BlsA is involved in synchronization to the zeitgeber during light-dark cycles. Importantly, β-lactamase activity varied along the day in cultures under light-dark period, establishing a new paradigm. Our work contributes to the developing field of circadian clocks in bacterial human pathogens, which could impact the microorganisms’ lifestyle and pathogenicity.
Fil: Mussi, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Permingeat, Valentin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Perez Mora, Barbara Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Migliori, María Laura. Universidad Nacional de Quilmes; Argentina
Fil: Arana, Natalia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Fernández, Julia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas.; Argentina
Fil: Allasia, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas.; Argentina
Fil: Lamberti, Melisa Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina
Fil: Di Venanzio, Gisela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of Washington. School of Medicine; Estados Unidos
Fil: Feldman, Mario. University of Washington. School of Medicine; Estados Unidos
Fil: Golombek, Diego Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de San Andrés; Argentina
Materia
ACINETOBACTER BAUMANNII
CIRCADIAN RHYTHM
PATHOGEN
ANTIBIOTIC SUSCEPTIBILITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/258304
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/258304
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Circadian rhythms in the critical human pathogen Acinetobacter baumanniiMussi, María AlejandraPermingeat, ValentinPerez Mora, Barbara EdithMigliori, María LauraArana, Natalia AlejandraFernández, JuliaAllasia, María BelénLamberti, Melisa LucianaDi Venanzio, Gisela AndreaFeldman, MarioGolombek, Diego AndrésACINETOBACTER BAUMANNIICIRCADIAN RHYTHMPATHOGENANTIBIOTIC SUSCEPTIBILITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Acinetobacter baumannii is recognized as the paradigm of multidrug resistant superbug, topping the WHO priority list of critical human pathogens. Interestingly, it senses and responds to blue light, which modulates global aspects of its physiology including the pathogenicity. We hypothesized that light could serve as a signal to synchronize the bacterial physiology to the host’s behavior, or to the environment. At environmental temperatures, light regulation is mainly governed by the BLUF-type photoreceptor BlsA. In this work, we identified the existence of daily rhythms in blsA expression displaying a robust response to light, as well as endogenous circadian rhythms in A. baumannii. In fact, we show that blsA gene expression can be synchronized to 24-hour blue light-dark cycles, which immediately resynchronizes after a phase shift due to a longer night. Upon release to constant darkness, bacterial populations present free-running oscillations with a period close to 24 hours. Furthermore, our data indicate that BlsA is involved in synchronization to the zeitgeber during light-dark cycles. Importantly, β-lactamase activity varied along the day in cultures under light-dark period, establishing a new paradigm. Our work contributes to the developing field of circadian clocks in bacterial human pathogens, which could impact the microorganisms’ lifestyle and pathogenicity.Fil: Mussi, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Permingeat, Valentin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Perez Mora, Barbara Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Migliori, María Laura. Universidad Nacional de Quilmes; ArgentinaFil: Arana, Natalia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Fernández, Julia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas.; ArgentinaFil: Allasia, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas.; ArgentinaFil: Lamberti, Melisa Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; ArgentinaFil: Di Venanzio, Gisela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of Washington. School of Medicine; Estados UnidosFil: Feldman, Mario. University of Washington. School of Medicine; Estados UnidosFil: Golombek, Diego Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de San Andrés; ArgentinaResearch Square Company2024-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/258304Mussi, María Alejandra; Permingeat, Valentin; Perez Mora, Barbara Edith; Migliori, María Laura; Arana, Natalia Alejandra; et al.; Circadian rhythms in the critical human pathogen Acinetobacter baumannii; Research Square Company; Research Square; 11-2024; 1-212693-5015CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.researchsquare.com/article/rs-5277866/v1info:eu-repo/semantics/altIdentifier/doi/10.21203/rs.3.rs-5277866/v1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:33:38Zoai:ri.conicet.gov.ar:11336/258304instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:33:38.553CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Circadian rhythms in the critical human pathogen Acinetobacter baumannii
title Circadian rhythms in the critical human pathogen Acinetobacter baumannii
spellingShingle Circadian rhythms in the critical human pathogen Acinetobacter baumannii
Mussi, María Alejandra
ACINETOBACTER BAUMANNII
CIRCADIAN RHYTHM
PATHOGEN
ANTIBIOTIC SUSCEPTIBILITY
title_short Circadian rhythms in the critical human pathogen Acinetobacter baumannii
title_full Circadian rhythms in the critical human pathogen Acinetobacter baumannii
title_fullStr Circadian rhythms in the critical human pathogen Acinetobacter baumannii
title_full_unstemmed Circadian rhythms in the critical human pathogen Acinetobacter baumannii
title_sort Circadian rhythms in the critical human pathogen Acinetobacter baumannii
dc.creator.none.fl_str_mv Mussi, María Alejandra
Permingeat, Valentin
Perez Mora, Barbara Edith
Migliori, María Laura
Arana, Natalia Alejandra
Fernández, Julia
Allasia, María Belén
Lamberti, Melisa Luciana
Di Venanzio, Gisela Andrea
Feldman, Mario
Golombek, Diego Andrés
author Mussi, María Alejandra
author_facet Mussi, María Alejandra
Permingeat, Valentin
Perez Mora, Barbara Edith
Migliori, María Laura
Arana, Natalia Alejandra
Fernández, Julia
Allasia, María Belén
Lamberti, Melisa Luciana
Di Venanzio, Gisela Andrea
Feldman, Mario
Golombek, Diego Andrés
author_role author
author2 Permingeat, Valentin
Perez Mora, Barbara Edith
Migliori, María Laura
Arana, Natalia Alejandra
Fernández, Julia
Allasia, María Belén
Lamberti, Melisa Luciana
Di Venanzio, Gisela Andrea
Feldman, Mario
Golombek, Diego Andrés
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ACINETOBACTER BAUMANNII
CIRCADIAN RHYTHM
PATHOGEN
ANTIBIOTIC SUSCEPTIBILITY
topic ACINETOBACTER BAUMANNII
CIRCADIAN RHYTHM
PATHOGEN
ANTIBIOTIC SUSCEPTIBILITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Acinetobacter baumannii is recognized as the paradigm of multidrug resistant superbug, topping the WHO priority list of critical human pathogens. Interestingly, it senses and responds to blue light, which modulates global aspects of its physiology including the pathogenicity. We hypothesized that light could serve as a signal to synchronize the bacterial physiology to the host’s behavior, or to the environment. At environmental temperatures, light regulation is mainly governed by the BLUF-type photoreceptor BlsA. In this work, we identified the existence of daily rhythms in blsA expression displaying a robust response to light, as well as endogenous circadian rhythms in A. baumannii. In fact, we show that blsA gene expression can be synchronized to 24-hour blue light-dark cycles, which immediately resynchronizes after a phase shift due to a longer night. Upon release to constant darkness, bacterial populations present free-running oscillations with a period close to 24 hours. Furthermore, our data indicate that BlsA is involved in synchronization to the zeitgeber during light-dark cycles. Importantly, β-lactamase activity varied along the day in cultures under light-dark period, establishing a new paradigm. Our work contributes to the developing field of circadian clocks in bacterial human pathogens, which could impact the microorganisms’ lifestyle and pathogenicity.
Fil: Mussi, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Permingeat, Valentin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Perez Mora, Barbara Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Migliori, María Laura. Universidad Nacional de Quilmes; Argentina
Fil: Arana, Natalia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Fernández, Julia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas.; Argentina
Fil: Allasia, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas.; Argentina
Fil: Lamberti, Melisa Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina
Fil: Di Venanzio, Gisela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of Washington. School of Medicine; Estados Unidos
Fil: Feldman, Mario. University of Washington. School of Medicine; Estados Unidos
Fil: Golombek, Diego Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de San Andrés; Argentina
description Acinetobacter baumannii is recognized as the paradigm of multidrug resistant superbug, topping the WHO priority list of critical human pathogens. Interestingly, it senses and responds to blue light, which modulates global aspects of its physiology including the pathogenicity. We hypothesized that light could serve as a signal to synchronize the bacterial physiology to the host’s behavior, or to the environment. At environmental temperatures, light regulation is mainly governed by the BLUF-type photoreceptor BlsA. In this work, we identified the existence of daily rhythms in blsA expression displaying a robust response to light, as well as endogenous circadian rhythms in A. baumannii. In fact, we show that blsA gene expression can be synchronized to 24-hour blue light-dark cycles, which immediately resynchronizes after a phase shift due to a longer night. Upon release to constant darkness, bacterial populations present free-running oscillations with a period close to 24 hours. Furthermore, our data indicate that BlsA is involved in synchronization to the zeitgeber during light-dark cycles. Importantly, β-lactamase activity varied along the day in cultures under light-dark period, establishing a new paradigm. Our work contributes to the developing field of circadian clocks in bacterial human pathogens, which could impact the microorganisms’ lifestyle and pathogenicity.
publishDate 2024
dc.date.none.fl_str_mv 2024-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/258304
Mussi, María Alejandra; Permingeat, Valentin; Perez Mora, Barbara Edith; Migliori, María Laura; Arana, Natalia Alejandra; et al.; Circadian rhythms in the critical human pathogen Acinetobacter baumannii; Research Square Company; Research Square; 11-2024; 1-21
2693-5015
CONICET Digital
CONICET
url http://hdl.handle.net/11336/258304
identifier_str_mv Mussi, María Alejandra; Permingeat, Valentin; Perez Mora, Barbara Edith; Migliori, María Laura; Arana, Natalia Alejandra; et al.; Circadian rhythms in the critical human pathogen Acinetobacter baumannii; Research Square Company; Research Square; 11-2024; 1-21
2693-5015
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.researchsquare.com/article/rs-5277866/v1
info:eu-repo/semantics/altIdentifier/doi/10.21203/rs.3.rs-5277866/v1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Research Square Company
publisher.none.fl_str_mv Research Square Company
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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