Nucleoredoxin Plays a Key Role in the Maintenance of Retinal Pigmented Epithelium Differentiation

Autores
Holubiec, Mariana Ines; Romero, Juan Ignacio; Urbainsky, Claudia; Gellert, Manuela; Galeano, Pablo; Capani, Francisco; Lillig, Christopher Horst; Hanschmann, Eva Maria
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nucleoredoxin (Nrx) belongs to the Thioredoxin protein family and functions in redox-mediated signal transduction. It contains the dithiol active site motif Cys-Pro-Pro-Cys and interacts and regulates different proteins in distinct cellular pathways. Nrx was shown to be catalytically active in the insulin assay and recent findings indicate that Nrx functions, in fact, as oxidase. Here, we have analyzed Nrx in the mammalian retina exposed to (perinatal) hypoxia-ischemia/reoxygenation, combining ex vivo and in vitro models. Our data show that Nrx regulates cell differentiation, which is important to (i) increase the number of glial cells and (ii) replenish neurons that are lost following the hypoxic insult. Nrx is essential to maintain cell morphology. These regulatory changes are related to VEGF but do not seem to be linked to the Wnt/β-catenin pathway, which is not affected by Nrx knock-down. In conclusion, our results strongly suggest that hypoxia-ischemia could lead to alterations in the organization of the retina, related to changes in RPE cell differentiation. Nrx may play an essential role in the maintenance of the RPE cell differentiation state via the regulation of VEGF release.
Fil: Holubiec, Mariana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Romero, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Urbainsky, Claudia. Ernst Moritz Arndt Universität Greifswald. Institut fur Geographie und Geologie; Alemania
Fil: Gellert, Manuela. Ernst Moritz Arndt Universität Greifswald. Institut fur Geographie und Geologie; Alemania
Fil: Galeano, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Capani, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina. Universidad Autonoma de Chile; Chile. Universidad Abierta Interamericana. Secretaría de Investigación. Centro de Altos Estudios En Ciencias Humanas y de la Salud - Sede Buenos Aires; Argentina
Fil: Lillig, Christopher Horst. Ernst Moritz Arndt Universität Greifswald. Institut fur Geographie und Geologie; Alemania
Fil: Hanschmann, Eva Maria. Ernst Moritz Arndt Universität Greifswald. Institut fur Geographie und Geologie; Alemania. Heinrich-Heine University; Alemania
Materia
DIFFERENTIATION
GLIA
HYPOXIA-ISCHEMIA
MORPHOLOGY
NEURONS
NUCLEOREDOXIN
REDOX REGULATION
REOXYGENATION
RETINA
RPE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/214766

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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Nucleoredoxin Plays a Key Role in the Maintenance of Retinal Pigmented Epithelium DifferentiationHolubiec, Mariana InesRomero, Juan IgnacioUrbainsky, ClaudiaGellert, ManuelaGaleano, PabloCapani, FranciscoLillig, Christopher HorstHanschmann, Eva MariaDIFFERENTIATIONGLIAHYPOXIA-ISCHEMIAMORPHOLOGYNEURONSNUCLEOREDOXINREDOX REGULATIONREOXYGENATIONRETINARPEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Nucleoredoxin (Nrx) belongs to the Thioredoxin protein family and functions in redox-mediated signal transduction. It contains the dithiol active site motif Cys-Pro-Pro-Cys and interacts and regulates different proteins in distinct cellular pathways. Nrx was shown to be catalytically active in the insulin assay and recent findings indicate that Nrx functions, in fact, as oxidase. Here, we have analyzed Nrx in the mammalian retina exposed to (perinatal) hypoxia-ischemia/reoxygenation, combining ex vivo and in vitro models. Our data show that Nrx regulates cell differentiation, which is important to (i) increase the number of glial cells and (ii) replenish neurons that are lost following the hypoxic insult. Nrx is essential to maintain cell morphology. These regulatory changes are related to VEGF but do not seem to be linked to the Wnt/β-catenin pathway, which is not affected by Nrx knock-down. In conclusion, our results strongly suggest that hypoxia-ischemia could lead to alterations in the organization of the retina, related to changes in RPE cell differentiation. Nrx may play an essential role in the maintenance of the RPE cell differentiation state via the regulation of VEGF release.Fil: Holubiec, Mariana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; ArgentinaFil: Romero, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Urbainsky, Claudia. Ernst Moritz Arndt Universität Greifswald. Institut fur Geographie und Geologie; AlemaniaFil: Gellert, Manuela. Ernst Moritz Arndt Universität Greifswald. Institut fur Geographie und Geologie; AlemaniaFil: Galeano, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Capani, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina. Universidad Autonoma de Chile; Chile. Universidad Abierta Interamericana. Secretaría de Investigación. Centro de Altos Estudios En Ciencias Humanas y de la Salud - Sede Buenos Aires; ArgentinaFil: Lillig, Christopher Horst. Ernst Moritz Arndt Universität Greifswald. Institut fur Geographie und Geologie; AlemaniaFil: Hanschmann, Eva Maria. Ernst Moritz Arndt Universität Greifswald. Institut fur Geographie und Geologie; Alemania. Heinrich-Heine University; AlemaniaMultidisciplinary Digital Publishing Institute2022-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/214766Holubiec, Mariana Ines; Romero, Juan Ignacio; Urbainsky, Claudia; Gellert, Manuela ; Galeano, Pablo; et al.; Nucleoredoxin Plays a Key Role in the Maintenance of Retinal Pigmented Epithelium Differentiation; Multidisciplinary Digital Publishing Institute; Antioxidants; 11; 6; 6-2022; 1-172076-3921CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-3921/11/6/1106info:eu-repo/semantics/altIdentifier/doi/10.3390/antiox11061106info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:28:14Zoai:ri.conicet.gov.ar:11336/214766instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:28:14.842CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nucleoredoxin Plays a Key Role in the Maintenance of Retinal Pigmented Epithelium Differentiation
title Nucleoredoxin Plays a Key Role in the Maintenance of Retinal Pigmented Epithelium Differentiation
spellingShingle Nucleoredoxin Plays a Key Role in the Maintenance of Retinal Pigmented Epithelium Differentiation
Holubiec, Mariana Ines
DIFFERENTIATION
GLIA
HYPOXIA-ISCHEMIA
MORPHOLOGY
NEURONS
NUCLEOREDOXIN
REDOX REGULATION
REOXYGENATION
RETINA
RPE
title_short Nucleoredoxin Plays a Key Role in the Maintenance of Retinal Pigmented Epithelium Differentiation
title_full Nucleoredoxin Plays a Key Role in the Maintenance of Retinal Pigmented Epithelium Differentiation
title_fullStr Nucleoredoxin Plays a Key Role in the Maintenance of Retinal Pigmented Epithelium Differentiation
title_full_unstemmed Nucleoredoxin Plays a Key Role in the Maintenance of Retinal Pigmented Epithelium Differentiation
title_sort Nucleoredoxin Plays a Key Role in the Maintenance of Retinal Pigmented Epithelium Differentiation
dc.creator.none.fl_str_mv Holubiec, Mariana Ines
Romero, Juan Ignacio
Urbainsky, Claudia
Gellert, Manuela
Galeano, Pablo
Capani, Francisco
Lillig, Christopher Horst
Hanschmann, Eva Maria
author Holubiec, Mariana Ines
author_facet Holubiec, Mariana Ines
Romero, Juan Ignacio
Urbainsky, Claudia
Gellert, Manuela
Galeano, Pablo
Capani, Francisco
Lillig, Christopher Horst
Hanschmann, Eva Maria
author_role author
author2 Romero, Juan Ignacio
Urbainsky, Claudia
Gellert, Manuela
Galeano, Pablo
Capani, Francisco
Lillig, Christopher Horst
Hanschmann, Eva Maria
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DIFFERENTIATION
GLIA
HYPOXIA-ISCHEMIA
MORPHOLOGY
NEURONS
NUCLEOREDOXIN
REDOX REGULATION
REOXYGENATION
RETINA
RPE
topic DIFFERENTIATION
GLIA
HYPOXIA-ISCHEMIA
MORPHOLOGY
NEURONS
NUCLEOREDOXIN
REDOX REGULATION
REOXYGENATION
RETINA
RPE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Nucleoredoxin (Nrx) belongs to the Thioredoxin protein family and functions in redox-mediated signal transduction. It contains the dithiol active site motif Cys-Pro-Pro-Cys and interacts and regulates different proteins in distinct cellular pathways. Nrx was shown to be catalytically active in the insulin assay and recent findings indicate that Nrx functions, in fact, as oxidase. Here, we have analyzed Nrx in the mammalian retina exposed to (perinatal) hypoxia-ischemia/reoxygenation, combining ex vivo and in vitro models. Our data show that Nrx regulates cell differentiation, which is important to (i) increase the number of glial cells and (ii) replenish neurons that are lost following the hypoxic insult. Nrx is essential to maintain cell morphology. These regulatory changes are related to VEGF but do not seem to be linked to the Wnt/β-catenin pathway, which is not affected by Nrx knock-down. In conclusion, our results strongly suggest that hypoxia-ischemia could lead to alterations in the organization of the retina, related to changes in RPE cell differentiation. Nrx may play an essential role in the maintenance of the RPE cell differentiation state via the regulation of VEGF release.
Fil: Holubiec, Mariana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Romero, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Urbainsky, Claudia. Ernst Moritz Arndt Universität Greifswald. Institut fur Geographie und Geologie; Alemania
Fil: Gellert, Manuela. Ernst Moritz Arndt Universität Greifswald. Institut fur Geographie und Geologie; Alemania
Fil: Galeano, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Capani, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina. Universidad Autonoma de Chile; Chile. Universidad Abierta Interamericana. Secretaría de Investigación. Centro de Altos Estudios En Ciencias Humanas y de la Salud - Sede Buenos Aires; Argentina
Fil: Lillig, Christopher Horst. Ernst Moritz Arndt Universität Greifswald. Institut fur Geographie und Geologie; Alemania
Fil: Hanschmann, Eva Maria. Ernst Moritz Arndt Universität Greifswald. Institut fur Geographie und Geologie; Alemania. Heinrich-Heine University; Alemania
description Nucleoredoxin (Nrx) belongs to the Thioredoxin protein family and functions in redox-mediated signal transduction. It contains the dithiol active site motif Cys-Pro-Pro-Cys and interacts and regulates different proteins in distinct cellular pathways. Nrx was shown to be catalytically active in the insulin assay and recent findings indicate that Nrx functions, in fact, as oxidase. Here, we have analyzed Nrx in the mammalian retina exposed to (perinatal) hypoxia-ischemia/reoxygenation, combining ex vivo and in vitro models. Our data show that Nrx regulates cell differentiation, which is important to (i) increase the number of glial cells and (ii) replenish neurons that are lost following the hypoxic insult. Nrx is essential to maintain cell morphology. These regulatory changes are related to VEGF but do not seem to be linked to the Wnt/β-catenin pathway, which is not affected by Nrx knock-down. In conclusion, our results strongly suggest that hypoxia-ischemia could lead to alterations in the organization of the retina, related to changes in RPE cell differentiation. Nrx may play an essential role in the maintenance of the RPE cell differentiation state via the regulation of VEGF release.
publishDate 2022
dc.date.none.fl_str_mv 2022-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/214766
Holubiec, Mariana Ines; Romero, Juan Ignacio; Urbainsky, Claudia; Gellert, Manuela ; Galeano, Pablo; et al.; Nucleoredoxin Plays a Key Role in the Maintenance of Retinal Pigmented Epithelium Differentiation; Multidisciplinary Digital Publishing Institute; Antioxidants; 11; 6; 6-2022; 1-17
2076-3921
CONICET Digital
CONICET
url http://hdl.handle.net/11336/214766
identifier_str_mv Holubiec, Mariana Ines; Romero, Juan Ignacio; Urbainsky, Claudia; Gellert, Manuela ; Galeano, Pablo; et al.; Nucleoredoxin Plays a Key Role in the Maintenance of Retinal Pigmented Epithelium Differentiation; Multidisciplinary Digital Publishing Institute; Antioxidants; 11; 6; 6-2022; 1-17
2076-3921
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-3921/11/6/1106
info:eu-repo/semantics/altIdentifier/doi/10.3390/antiox11061106
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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