Involvement of glutamate in retinal protection against ischemia/reperfusion damage induced by post-conditioning.

Autores
Fernández, Diego Carlos; Chianelli, Mónica Silvia; Rosenstein, Ruth Estela
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Retinal ischemia could provoke blindness and there is no effective treatment against retinal ischemic damage. Brief intermittent ischemia applied during the onset of reperfusion (i.e., postconditioning) protects the retina from ischemia/reperfusion injury. Multiple evidences support that glutamate is implicated in retinal ischemic damage. We investigated the involvement of glutamate clearance in postconditioning-induced protection. For this purpose, ischemia was induced by increasing intraocular pressure for 40 min, and 5 min after reperfusion, animals underwent 7 cycles of 1min/1min ischemia/reperfusion. One, 3, or 7 days after ischemia, animals were subjected to electroretinography and histological analysis. The functional and histological protection induced by postconditioning was evident at 7 (but not 1 or 3) days post-ischemia. An increase in Müller cell glial fibrillary acidic protein (GFAP) levels was observed at 1, 3, and 7 days after ischemia, whereas postconditioning reduced Müller cells GFAP levels at 3 and 7 days post-ischemia. Three days after ischemia, a significant decrease in glutamate uptake and glutamine synthetase activity was observed, whereas postconditioning reversed the effect of ischemia. The intravitreal injection of supraphysiological levels of glutamate mimicked electroretinographic and histological alterations provoked by ischemia, which were abrogated by postconditioning. These results support the involvement of glutamate in retinal protection against ischemia/reperfusion damage induced by postconditioning.
Fil: Fernández, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
Fil: Chianelli, Mónica Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
Fil: Rosenstein, Ruth Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
Materia
RETINA
ISCHEMIA
POSTCONDITIONING
GLUTAMATE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/123219
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/123219
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Involvement of glutamate in retinal protection against ischemia/reperfusion damage induced by post-conditioning.Fernández, Diego CarlosChianelli, Mónica SilviaRosenstein, Ruth EstelaRETINAISCHEMIAPOSTCONDITIONINGGLUTAMATEhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Retinal ischemia could provoke blindness and there is no effective treatment against retinal ischemic damage. Brief intermittent ischemia applied during the onset of reperfusion (i.e., postconditioning) protects the retina from ischemia/reperfusion injury. Multiple evidences support that glutamate is implicated in retinal ischemic damage. We investigated the involvement of glutamate clearance in postconditioning-induced protection. For this purpose, ischemia was induced by increasing intraocular pressure for 40 min, and 5 min after reperfusion, animals underwent 7 cycles of 1min/1min ischemia/reperfusion. One, 3, or 7 days after ischemia, animals were subjected to electroretinography and histological analysis. The functional and histological protection induced by postconditioning was evident at 7 (but not 1 or 3) days post-ischemia. An increase in Müller cell glial fibrillary acidic protein (GFAP) levels was observed at 1, 3, and 7 days after ischemia, whereas postconditioning reduced Müller cells GFAP levels at 3 and 7 days post-ischemia. Three days after ischemia, a significant decrease in glutamate uptake and glutamine synthetase activity was observed, whereas postconditioning reversed the effect of ischemia. The intravitreal injection of supraphysiological levels of glutamate mimicked electroretinographic and histological alterations provoked by ischemia, which were abrogated by postconditioning. These results support the involvement of glutamate in retinal protection against ischemia/reperfusion damage induced by postconditioning.Fil: Fernández, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; ArgentinaFil: Chianelli, Mónica Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; ArgentinaFil: Rosenstein, Ruth Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; ArgentinaWiley Blackwell Publishing, Inc2009-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/123219Fernández, Diego Carlos; Chianelli, Mónica Silvia; Rosenstein, Ruth Estela; Involvement of glutamate in retinal protection against ischemia/reperfusion damage induced by post-conditioning.; Wiley Blackwell Publishing, Inc; Journal of Neurochemistry; 111; 2; 12-2009; 488-4980022-3042CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1471-4159.2009.06334.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1471-4159.2009.06334.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:14Zoai:ri.conicet.gov.ar:11336/123219instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:14.44CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Involvement of glutamate in retinal protection against ischemia/reperfusion damage induced by post-conditioning.
title Involvement of glutamate in retinal protection against ischemia/reperfusion damage induced by post-conditioning.
spellingShingle Involvement of glutamate in retinal protection against ischemia/reperfusion damage induced by post-conditioning.
Fernández, Diego Carlos
RETINA
ISCHEMIA
POSTCONDITIONING
GLUTAMATE
title_short Involvement of glutamate in retinal protection against ischemia/reperfusion damage induced by post-conditioning.
title_full Involvement of glutamate in retinal protection against ischemia/reperfusion damage induced by post-conditioning.
title_fullStr Involvement of glutamate in retinal protection against ischemia/reperfusion damage induced by post-conditioning.
title_full_unstemmed Involvement of glutamate in retinal protection against ischemia/reperfusion damage induced by post-conditioning.
title_sort Involvement of glutamate in retinal protection against ischemia/reperfusion damage induced by post-conditioning.
dc.creator.none.fl_str_mv Fernández, Diego Carlos
Chianelli, Mónica Silvia
Rosenstein, Ruth Estela
author Fernández, Diego Carlos
author_facet Fernández, Diego Carlos
Chianelli, Mónica Silvia
Rosenstein, Ruth Estela
author_role author
author2 Chianelli, Mónica Silvia
Rosenstein, Ruth Estela
author2_role author
author
dc.subject.none.fl_str_mv RETINA
ISCHEMIA
POSTCONDITIONING
GLUTAMATE
topic RETINA
ISCHEMIA
POSTCONDITIONING
GLUTAMATE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Retinal ischemia could provoke blindness and there is no effective treatment against retinal ischemic damage. Brief intermittent ischemia applied during the onset of reperfusion (i.e., postconditioning) protects the retina from ischemia/reperfusion injury. Multiple evidences support that glutamate is implicated in retinal ischemic damage. We investigated the involvement of glutamate clearance in postconditioning-induced protection. For this purpose, ischemia was induced by increasing intraocular pressure for 40 min, and 5 min after reperfusion, animals underwent 7 cycles of 1min/1min ischemia/reperfusion. One, 3, or 7 days after ischemia, animals were subjected to electroretinography and histological analysis. The functional and histological protection induced by postconditioning was evident at 7 (but not 1 or 3) days post-ischemia. An increase in Müller cell glial fibrillary acidic protein (GFAP) levels was observed at 1, 3, and 7 days after ischemia, whereas postconditioning reduced Müller cells GFAP levels at 3 and 7 days post-ischemia. Three days after ischemia, a significant decrease in glutamate uptake and glutamine synthetase activity was observed, whereas postconditioning reversed the effect of ischemia. The intravitreal injection of supraphysiological levels of glutamate mimicked electroretinographic and histological alterations provoked by ischemia, which were abrogated by postconditioning. These results support the involvement of glutamate in retinal protection against ischemia/reperfusion damage induced by postconditioning.
Fil: Fernández, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
Fil: Chianelli, Mónica Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
Fil: Rosenstein, Ruth Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
description Retinal ischemia could provoke blindness and there is no effective treatment against retinal ischemic damage. Brief intermittent ischemia applied during the onset of reperfusion (i.e., postconditioning) protects the retina from ischemia/reperfusion injury. Multiple evidences support that glutamate is implicated in retinal ischemic damage. We investigated the involvement of glutamate clearance in postconditioning-induced protection. For this purpose, ischemia was induced by increasing intraocular pressure for 40 min, and 5 min after reperfusion, animals underwent 7 cycles of 1min/1min ischemia/reperfusion. One, 3, or 7 days after ischemia, animals were subjected to electroretinography and histological analysis. The functional and histological protection induced by postconditioning was evident at 7 (but not 1 or 3) days post-ischemia. An increase in Müller cell glial fibrillary acidic protein (GFAP) levels was observed at 1, 3, and 7 days after ischemia, whereas postconditioning reduced Müller cells GFAP levels at 3 and 7 days post-ischemia. Three days after ischemia, a significant decrease in glutamate uptake and glutamine synthetase activity was observed, whereas postconditioning reversed the effect of ischemia. The intravitreal injection of supraphysiological levels of glutamate mimicked electroretinographic and histological alterations provoked by ischemia, which were abrogated by postconditioning. These results support the involvement of glutamate in retinal protection against ischemia/reperfusion damage induced by postconditioning.
publishDate 2009
dc.date.none.fl_str_mv 2009-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/123219
Fernández, Diego Carlos; Chianelli, Mónica Silvia; Rosenstein, Ruth Estela; Involvement of glutamate in retinal protection against ischemia/reperfusion damage induced by post-conditioning.; Wiley Blackwell Publishing, Inc; Journal of Neurochemistry; 111; 2; 12-2009; 488-498
0022-3042
CONICET Digital
CONICET
url http://hdl.handle.net/11336/123219
identifier_str_mv Fernández, Diego Carlos; Chianelli, Mónica Silvia; Rosenstein, Ruth Estela; Involvement of glutamate in retinal protection against ischemia/reperfusion damage induced by post-conditioning.; Wiley Blackwell Publishing, Inc; Journal of Neurochemistry; 111; 2; 12-2009; 488-498
0022-3042
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1471-4159.2009.06334.x
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1471-4159.2009.06334.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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