Hypothermia Prevents Nitric Oxide System Changes in Retina Induced by Severe Perinatal Asphyxia

Autores
Rey Funes, Manuel; Ibarra, Mariano Esteban; Dorfman, Verónica Berta; Serrano, Julia; Fernández, Ana Patricia; Martínez Murillo, Ricardo; Martínez, Alfredo; Coirini, Hector; Rodrigo, José; Loidl, Cesar Fabian
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
One-third of asphyctic neonates develop long-term neurological injuries, including several degrees of ischemic proliferative retinopathy (IPR) such as retinopathy of prematurity (ROP). Given that the retina is altered by perinatal asphyxia, our aim was to study the effects of nitric oxide (NO) in the retina in order to analyze its impact on the retinal injury. Application of hypothermia was evaluated as preventive treatment. Sprague-Dawley rats were subjected to perinatal asphyxia [either at 37°C (PA group) or at 15°C (HYP group)]. Full-term rats were used as controls (CTL). A significantly increased activity of both constitutive NO synthase (nNOS, Ca2+-dependent) and inducible NO synthase (iNOS, Ca2+-independent) was observed in PA retinas from 21 days old up to 60 days old with respect to age-matched CTL, with a significant increase along the time course in the PA. nNOS was immunolocalized at amacrine, horizontal, and ganglion cells of the PA group, with a significant increase in relative optical density (R.O.D.), cellular area, and number of cells. iNOS immunoreactivity was observed in the inner nuclear layer and in the internal Müller cell processes of PA, with a significant increase in R.O.D. and colocalizing with GFAP in the 60-day-old PA group. Six nitrated protein species were increased in retinas from PA rats. Nitrotyrosine immunoreactivity showed a localization similar to that of iNOS, with increased R.O.D. in the PA group and colocalization with GFAP in 60-day-old animals. HYP prevented all the changes observed in PA rats. Although the NO system displays changes induced by hypoxia-ischemia, hypothermia application shows a strong protective effect.
Fil: Rey Funes, Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Ibarra, Mariano Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Dorfman, Verónica Berta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Serrano, Julia. Instituto Cajal; España. Consejo Superior de Investigaciones Científicas; España
Fil: Fernández, Ana Patricia. Instituto Cajal; España. Consejo Superior de Investigaciones Científicas; España
Fil: Martínez Murillo, Ricardo. Instituto Cajal; España. Consejo Superior de Investigaciones Científicas; España
Fil: Martínez, Alfredo. Instituto Cajal; España. Consejo Superior de Investigaciones Científicas; España
Fil: Coirini, Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
Fil: Rodrigo, José. Instituto Cajal; España. Consejo Superior de Investigaciones Científicas; España
Fil: Loidl, Cesar Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Materia
Retina
Hypoxia-Ischemia
Rop
Nitrotyrosine
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/29192
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/29192
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Hypothermia Prevents Nitric Oxide System Changes in Retina Induced by Severe Perinatal AsphyxiaRey Funes, ManuelIbarra, Mariano EstebanDorfman, Verónica BertaSerrano, JuliaFernández, Ana PatriciaMartínez Murillo, RicardoMartínez, AlfredoCoirini, HectorRodrigo, JoséLoidl, Cesar FabianRetinaHypoxia-IschemiaRopNitrotyrosinehttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3One-third of asphyctic neonates develop long-term neurological injuries, including several degrees of ischemic proliferative retinopathy (IPR) such as retinopathy of prematurity (ROP). Given that the retina is altered by perinatal asphyxia, our aim was to study the effects of nitric oxide (NO) in the retina in order to analyze its impact on the retinal injury. Application of hypothermia was evaluated as preventive treatment. Sprague-Dawley rats were subjected to perinatal asphyxia [either at 37°C (PA group) or at 15°C (HYP group)]. Full-term rats were used as controls (CTL). A significantly increased activity of both constitutive NO synthase (nNOS, Ca2+-dependent) and inducible NO synthase (iNOS, Ca2+-independent) was observed in PA retinas from 21 days old up to 60 days old with respect to age-matched CTL, with a significant increase along the time course in the PA. nNOS was immunolocalized at amacrine, horizontal, and ganglion cells of the PA group, with a significant increase in relative optical density (R.O.D.), cellular area, and number of cells. iNOS immunoreactivity was observed in the inner nuclear layer and in the internal Müller cell processes of PA, with a significant increase in R.O.D. and colocalizing with GFAP in the 60-day-old PA group. Six nitrated protein species were increased in retinas from PA rats. Nitrotyrosine immunoreactivity showed a localization similar to that of iNOS, with increased R.O.D. in the PA group and colocalization with GFAP in 60-day-old animals. HYP prevented all the changes observed in PA rats. Although the NO system displays changes induced by hypoxia-ischemia, hypothermia application shows a strong protective effect.Fil: Rey Funes, Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaFil: Ibarra, Mariano Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaFil: Dorfman, Verónica Berta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaFil: Serrano, Julia. Instituto Cajal; España. Consejo Superior de Investigaciones Científicas; EspañaFil: Fernández, Ana Patricia. Instituto Cajal; España. Consejo Superior de Investigaciones Científicas; EspañaFil: Martínez Murillo, Ricardo. Instituto Cajal; España. Consejo Superior de Investigaciones Científicas; EspañaFil: Martínez, Alfredo. Instituto Cajal; España. Consejo Superior de Investigaciones Científicas; EspañaFil: Coirini, Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; ArgentinaFil: Rodrigo, José. Instituto Cajal; España. Consejo Superior de Investigaciones Científicas; EspañaFil: Loidl, Cesar Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaWiley2011-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/29192Rey Funes, Manuel; Ibarra, Mariano Esteban; Dorfman, Verónica Berta; Serrano, Julia; Fernández, Ana Patricia; et al.; Hypothermia Prevents Nitric Oxide System Changes in Retina Induced by Severe Perinatal Asphyxia; Wiley; Journal of Neuroscience Research; 89; 5; 2-2011; 729-7430360-40121097-4547CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/jnr.22556info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jnr.22556/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:12:03Zoai:ri.conicet.gov.ar:11336/29192instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:12:03.921CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Hypothermia Prevents Nitric Oxide System Changes in Retina Induced by Severe Perinatal Asphyxia
title Hypothermia Prevents Nitric Oxide System Changes in Retina Induced by Severe Perinatal Asphyxia
spellingShingle Hypothermia Prevents Nitric Oxide System Changes in Retina Induced by Severe Perinatal Asphyxia
Rey Funes, Manuel
Retina
Hypoxia-Ischemia
Rop
Nitrotyrosine
title_short Hypothermia Prevents Nitric Oxide System Changes in Retina Induced by Severe Perinatal Asphyxia
title_full Hypothermia Prevents Nitric Oxide System Changes in Retina Induced by Severe Perinatal Asphyxia
title_fullStr Hypothermia Prevents Nitric Oxide System Changes in Retina Induced by Severe Perinatal Asphyxia
title_full_unstemmed Hypothermia Prevents Nitric Oxide System Changes in Retina Induced by Severe Perinatal Asphyxia
title_sort Hypothermia Prevents Nitric Oxide System Changes in Retina Induced by Severe Perinatal Asphyxia
dc.creator.none.fl_str_mv Rey Funes, Manuel
Ibarra, Mariano Esteban
Dorfman, Verónica Berta
Serrano, Julia
Fernández, Ana Patricia
Martínez Murillo, Ricardo
Martínez, Alfredo
Coirini, Hector
Rodrigo, José
Loidl, Cesar Fabian
author Rey Funes, Manuel
author_facet Rey Funes, Manuel
Ibarra, Mariano Esteban
Dorfman, Verónica Berta
Serrano, Julia
Fernández, Ana Patricia
Martínez Murillo, Ricardo
Martínez, Alfredo
Coirini, Hector
Rodrigo, José
Loidl, Cesar Fabian
author_role author
author2 Ibarra, Mariano Esteban
Dorfman, Verónica Berta
Serrano, Julia
Fernández, Ana Patricia
Martínez Murillo, Ricardo
Martínez, Alfredo
Coirini, Hector
Rodrigo, José
Loidl, Cesar Fabian
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Retina
Hypoxia-Ischemia
Rop
Nitrotyrosine
topic Retina
Hypoxia-Ischemia
Rop
Nitrotyrosine
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv One-third of asphyctic neonates develop long-term neurological injuries, including several degrees of ischemic proliferative retinopathy (IPR) such as retinopathy of prematurity (ROP). Given that the retina is altered by perinatal asphyxia, our aim was to study the effects of nitric oxide (NO) in the retina in order to analyze its impact on the retinal injury. Application of hypothermia was evaluated as preventive treatment. Sprague-Dawley rats were subjected to perinatal asphyxia [either at 37°C (PA group) or at 15°C (HYP group)]. Full-term rats were used as controls (CTL). A significantly increased activity of both constitutive NO synthase (nNOS, Ca2+-dependent) and inducible NO synthase (iNOS, Ca2+-independent) was observed in PA retinas from 21 days old up to 60 days old with respect to age-matched CTL, with a significant increase along the time course in the PA. nNOS was immunolocalized at amacrine, horizontal, and ganglion cells of the PA group, with a significant increase in relative optical density (R.O.D.), cellular area, and number of cells. iNOS immunoreactivity was observed in the inner nuclear layer and in the internal Müller cell processes of PA, with a significant increase in R.O.D. and colocalizing with GFAP in the 60-day-old PA group. Six nitrated protein species were increased in retinas from PA rats. Nitrotyrosine immunoreactivity showed a localization similar to that of iNOS, with increased R.O.D. in the PA group and colocalization with GFAP in 60-day-old animals. HYP prevented all the changes observed in PA rats. Although the NO system displays changes induced by hypoxia-ischemia, hypothermia application shows a strong protective effect.
Fil: Rey Funes, Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Ibarra, Mariano Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Dorfman, Verónica Berta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Serrano, Julia. Instituto Cajal; España. Consejo Superior de Investigaciones Científicas; España
Fil: Fernández, Ana Patricia. Instituto Cajal; España. Consejo Superior de Investigaciones Científicas; España
Fil: Martínez Murillo, Ricardo. Instituto Cajal; España. Consejo Superior de Investigaciones Científicas; España
Fil: Martínez, Alfredo. Instituto Cajal; España. Consejo Superior de Investigaciones Científicas; España
Fil: Coirini, Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
Fil: Rodrigo, José. Instituto Cajal; España. Consejo Superior de Investigaciones Científicas; España
Fil: Loidl, Cesar Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
description One-third of asphyctic neonates develop long-term neurological injuries, including several degrees of ischemic proliferative retinopathy (IPR) such as retinopathy of prematurity (ROP). Given that the retina is altered by perinatal asphyxia, our aim was to study the effects of nitric oxide (NO) in the retina in order to analyze its impact on the retinal injury. Application of hypothermia was evaluated as preventive treatment. Sprague-Dawley rats were subjected to perinatal asphyxia [either at 37°C (PA group) or at 15°C (HYP group)]. Full-term rats were used as controls (CTL). A significantly increased activity of both constitutive NO synthase (nNOS, Ca2+-dependent) and inducible NO synthase (iNOS, Ca2+-independent) was observed in PA retinas from 21 days old up to 60 days old with respect to age-matched CTL, with a significant increase along the time course in the PA. nNOS was immunolocalized at amacrine, horizontal, and ganglion cells of the PA group, with a significant increase in relative optical density (R.O.D.), cellular area, and number of cells. iNOS immunoreactivity was observed in the inner nuclear layer and in the internal Müller cell processes of PA, with a significant increase in R.O.D. and colocalizing with GFAP in the 60-day-old PA group. Six nitrated protein species were increased in retinas from PA rats. Nitrotyrosine immunoreactivity showed a localization similar to that of iNOS, with increased R.O.D. in the PA group and colocalization with GFAP in 60-day-old animals. HYP prevented all the changes observed in PA rats. Although the NO system displays changes induced by hypoxia-ischemia, hypothermia application shows a strong protective effect.
publishDate 2011
dc.date.none.fl_str_mv 2011-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/29192
Rey Funes, Manuel; Ibarra, Mariano Esteban; Dorfman, Verónica Berta; Serrano, Julia; Fernández, Ana Patricia; et al.; Hypothermia Prevents Nitric Oxide System Changes in Retina Induced by Severe Perinatal Asphyxia; Wiley; Journal of Neuroscience Research; 89; 5; 2-2011; 729-743
0360-4012
1097-4547
CONICET Digital
CONICET
url http://hdl.handle.net/11336/29192
identifier_str_mv Rey Funes, Manuel; Ibarra, Mariano Esteban; Dorfman, Verónica Berta; Serrano, Julia; Fernández, Ana Patricia; et al.; Hypothermia Prevents Nitric Oxide System Changes in Retina Induced by Severe Perinatal Asphyxia; Wiley; Journal of Neuroscience Research; 89; 5; 2-2011; 729-743
0360-4012
1097-4547
CONICET Digital
CONICET
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language eng
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info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jnr.22556/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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