Induction of Topographical changes in Poly-ε-Caprolactone scaffolds for bone tissue engineering: biocompatibility and cytotoxicity evaluations

Autores
Fernandez, Juan Manuel; Alfano, Ana Laura
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bone tissue engineering (BTE) uses principles from different fields, such as medicine, biochemistry and engineering, in order to restore or improve damaged tissue. Topographic changes of Poly-ε-caprolactone scaffolds (PCL) have been previously induced by exposure of the polymer to various concentrations of NaOH for different periods of time. However, lack of consistency between the treatment conditions used by different research groups has led to inconsistent results, with no clear conclusion arising regarding the benefits of these treatments. The aim of this study was to evaluate how different treatments (time-concentration) with NaOH could affect its biocompatibility for bone marrow stromal cells (BMSC) and its cytotoxicity towards RAW 264.7 macrophages of a PCL scaffold. We also analyzed the physicochemical and mechanical properties of the PCL films after different treatment. We have shown that treatment with for 2 hours with NaOH produced changes that affected the hydrophobicity and biocompatibility of the scaffold by increasing the proliferation and ALP activity in cells grown on them. Beside, 24 hours of treatment with NaOH produced significant decreased in mechanical properties resulting in a scaffold highly fragile and low biocompatibility compared to PCL. Therefore, treatment for 2 hours with NaOH can be used to increase the biocompatibility of PCL scaffolds.
Fil: Fernandez, Juan Manuel. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigacion En Osteospatias y Metabolismo Mineral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alfano, Ana Laura. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigacion En Osteospatias y Metabolismo Mineral; Argentina
Materia
Cytotoxicity
Raw 264.7 Macrophages
Bone Tissue Engineering
Poly-Epsilon-Caprolactone
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13743
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Induction of Topographical changes in Poly-ε-Caprolactone scaffolds for bone tissue engineering: biocompatibility and cytotoxicity evaluationsFernandez, Juan ManuelAlfano, Ana LauraCytotoxicityRaw 264.7 MacrophagesBone Tissue EngineeringPoly-Epsilon-Caprolactonehttps://purl.org/becyt/ford/2.5https://purl.org/becyt/ford/2Bone tissue engineering (BTE) uses principles from different fields, such as medicine, biochemistry and engineering, in order to restore or improve damaged tissue. Topographic changes of Poly-ε-caprolactone scaffolds (PCL) have been previously induced by exposure of the polymer to various concentrations of NaOH for different periods of time. However, lack of consistency between the treatment conditions used by different research groups has led to inconsistent results, with no clear conclusion arising regarding the benefits of these treatments. The aim of this study was to evaluate how different treatments (time-concentration) with NaOH could affect its biocompatibility for bone marrow stromal cells (BMSC) and its cytotoxicity towards RAW 264.7 macrophages of a PCL scaffold. We also analyzed the physicochemical and mechanical properties of the PCL films after different treatment. We have shown that treatment with for 2 hours with NaOH produced changes that affected the hydrophobicity and biocompatibility of the scaffold by increasing the proliferation and ALP activity in cells grown on them. Beside, 24 hours of treatment with NaOH produced significant decreased in mechanical properties resulting in a scaffold highly fragile and low biocompatibility compared to PCL. Therefore, treatment for 2 hours with NaOH can be used to increase the biocompatibility of PCL scaffolds.Fil: Fernandez, Juan Manuel. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigacion En Osteospatias y Metabolismo Mineral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alfano, Ana Laura. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigacion En Osteospatias y Metabolismo Mineral; ArgentinaAmerican Scientific Publishers2015-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13743Fernandez, Juan Manuel; Alfano, Ana Laura; Induction of Topographical changes in Poly-ε-Caprolactone scaffolds for bone tissue engineering: biocompatibility and cytotoxicity evaluations; American Scientific Publishers; Journal of Biomaterials and Tissue Engineering; 5; 2; 2-2015; 142-1492157-9083enginfo:eu-repo/semantics/altIdentifier/doi/10.1166/jbt.2015.1290info:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/content/asp/jbte/2015/00000005/00000002/art00009info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:14Zoai:ri.conicet.gov.ar:11336/13743instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:14.31CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Induction of Topographical changes in Poly-ε-Caprolactone scaffolds for bone tissue engineering: biocompatibility and cytotoxicity evaluations
title Induction of Topographical changes in Poly-ε-Caprolactone scaffolds for bone tissue engineering: biocompatibility and cytotoxicity evaluations
spellingShingle Induction of Topographical changes in Poly-ε-Caprolactone scaffolds for bone tissue engineering: biocompatibility and cytotoxicity evaluations
Fernandez, Juan Manuel
Cytotoxicity
Raw 264.7 Macrophages
Bone Tissue Engineering
Poly-Epsilon-Caprolactone
title_short Induction of Topographical changes in Poly-ε-Caprolactone scaffolds for bone tissue engineering: biocompatibility and cytotoxicity evaluations
title_full Induction of Topographical changes in Poly-ε-Caprolactone scaffolds for bone tissue engineering: biocompatibility and cytotoxicity evaluations
title_fullStr Induction of Topographical changes in Poly-ε-Caprolactone scaffolds for bone tissue engineering: biocompatibility and cytotoxicity evaluations
title_full_unstemmed Induction of Topographical changes in Poly-ε-Caprolactone scaffolds for bone tissue engineering: biocompatibility and cytotoxicity evaluations
title_sort Induction of Topographical changes in Poly-ε-Caprolactone scaffolds for bone tissue engineering: biocompatibility and cytotoxicity evaluations
dc.creator.none.fl_str_mv Fernandez, Juan Manuel
Alfano, Ana Laura
author Fernandez, Juan Manuel
author_facet Fernandez, Juan Manuel
Alfano, Ana Laura
author_role author
author2 Alfano, Ana Laura
author2_role author
dc.subject.none.fl_str_mv Cytotoxicity
Raw 264.7 Macrophages
Bone Tissue Engineering
Poly-Epsilon-Caprolactone
topic Cytotoxicity
Raw 264.7 Macrophages
Bone Tissue Engineering
Poly-Epsilon-Caprolactone
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.5
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Bone tissue engineering (BTE) uses principles from different fields, such as medicine, biochemistry and engineering, in order to restore or improve damaged tissue. Topographic changes of Poly-ε-caprolactone scaffolds (PCL) have been previously induced by exposure of the polymer to various concentrations of NaOH for different periods of time. However, lack of consistency between the treatment conditions used by different research groups has led to inconsistent results, with no clear conclusion arising regarding the benefits of these treatments. The aim of this study was to evaluate how different treatments (time-concentration) with NaOH could affect its biocompatibility for bone marrow stromal cells (BMSC) and its cytotoxicity towards RAW 264.7 macrophages of a PCL scaffold. We also analyzed the physicochemical and mechanical properties of the PCL films after different treatment. We have shown that treatment with for 2 hours with NaOH produced changes that affected the hydrophobicity and biocompatibility of the scaffold by increasing the proliferation and ALP activity in cells grown on them. Beside, 24 hours of treatment with NaOH produced significant decreased in mechanical properties resulting in a scaffold highly fragile and low biocompatibility compared to PCL. Therefore, treatment for 2 hours with NaOH can be used to increase the biocompatibility of PCL scaffolds.
Fil: Fernandez, Juan Manuel. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigacion En Osteospatias y Metabolismo Mineral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alfano, Ana Laura. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigacion En Osteospatias y Metabolismo Mineral; Argentina
description Bone tissue engineering (BTE) uses principles from different fields, such as medicine, biochemistry and engineering, in order to restore or improve damaged tissue. Topographic changes of Poly-ε-caprolactone scaffolds (PCL) have been previously induced by exposure of the polymer to various concentrations of NaOH for different periods of time. However, lack of consistency between the treatment conditions used by different research groups has led to inconsistent results, with no clear conclusion arising regarding the benefits of these treatments. The aim of this study was to evaluate how different treatments (time-concentration) with NaOH could affect its biocompatibility for bone marrow stromal cells (BMSC) and its cytotoxicity towards RAW 264.7 macrophages of a PCL scaffold. We also analyzed the physicochemical and mechanical properties of the PCL films after different treatment. We have shown that treatment with for 2 hours with NaOH produced changes that affected the hydrophobicity and biocompatibility of the scaffold by increasing the proliferation and ALP activity in cells grown on them. Beside, 24 hours of treatment with NaOH produced significant decreased in mechanical properties resulting in a scaffold highly fragile and low biocompatibility compared to PCL. Therefore, treatment for 2 hours with NaOH can be used to increase the biocompatibility of PCL scaffolds.
publishDate 2015
dc.date.none.fl_str_mv 2015-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13743
Fernandez, Juan Manuel; Alfano, Ana Laura; Induction of Topographical changes in Poly-ε-Caprolactone scaffolds for bone tissue engineering: biocompatibility and cytotoxicity evaluations; American Scientific Publishers; Journal of Biomaterials and Tissue Engineering; 5; 2; 2-2015; 142-149
2157-9083
url http://hdl.handle.net/11336/13743
identifier_str_mv Fernandez, Juan Manuel; Alfano, Ana Laura; Induction of Topographical changes in Poly-ε-Caprolactone scaffolds for bone tissue engineering: biocompatibility and cytotoxicity evaluations; American Scientific Publishers; Journal of Biomaterials and Tissue Engineering; 5; 2; 2-2015; 142-149
2157-9083
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1166/jbt.2015.1290
info:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/content/asp/jbte/2015/00000005/00000002/art00009
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Scientific Publishers
publisher.none.fl_str_mv American Scientific Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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