Evaluation of strontium-containing PCL-PDIPF scaffolds for bone tissue engineering: In vitro and in vivo studies

Autores
Lino, Agustina Berenice; McCarthy, Antonio Desmond; Fernández, Juan Manuel
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bone tissue engineering (BTE) has the general objective of restoring and improving damaged bone. A very interesting strategy for BTE is to combine an adequate polymeric scaffold with an osteoinductive compound. Strontium is a divalent cation that can substitute calcium in hydroxyapatite and induce both anabolic and anti-catabolic effects in bone. On the other hand, systemic increases in Sr 2+ levels can provoke adverse cardiovascular effects. In the present study we have developed a compatibilized blend of poly-ε-caprolactone (PCL) and polydiisopropyl fumarate (PDIPF) enriched with 1% or 5% Sr 2+ and evaluated the applicability of these biomaterials for BTE, both in vitro and in vivo. In vitro, whereas Blend + 5% Sr 2+ was pro-inflammatory and anti-osteogenic, Blend + 1% Sr 2+ released very low quantities of the cation; was not cytotoxic for cultured macrophages; and showed improved osteocompatibility when used as a substratum for primary cultures of bone marrow stromal cells. In vivo, implants with Blend + 1% Sr 2+ significantly increased bone tissue regeneration and improved fibrous bridging (vs. Blend alone), while neither inducing a local inflammatory response nor increased serum levels of Sr 2+ . These results indicate that our compatibilized blend of PCL-PDIPF enriched with 1% Sr 2+ could be useful for BTE.
Fil: Lino, Agustina Berenice. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: McCarthy, Antonio Desmond. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina
Fil: Fernández, Juan Manuel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Materia
BONE MARROW STROMAL CELLS
BONE REGENERATION
POLY-Ε-CAPROLACTONE
POLYDIISOPROPYL FUMARATE
RAW 264.7 MACROPHAGES
STRONTIUM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/131911
Acceder
id CONICETDig_cd10033f01fbe2dca8c20c1242d5bfce
oai_identifier_str oai:ri.conicet.gov.ar:11336/131911
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of strontium-containing PCL-PDIPF scaffolds for bone tissue engineering: In vitro and in vivo studiesLino, Agustina BereniceMcCarthy, Antonio DesmondFernández, Juan ManuelBONE MARROW STROMAL CELLSBONE REGENERATIONPOLY-Ε-CAPROLACTONEPOLYDIISOPROPYL FUMARATERAW 264.7 MACROPHAGESSTRONTIUMhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Bone tissue engineering (BTE) has the general objective of restoring and improving damaged bone. A very interesting strategy for BTE is to combine an adequate polymeric scaffold with an osteoinductive compound. Strontium is a divalent cation that can substitute calcium in hydroxyapatite and induce both anabolic and anti-catabolic effects in bone. On the other hand, systemic increases in Sr 2+ levels can provoke adverse cardiovascular effects. In the present study we have developed a compatibilized blend of poly-ε-caprolactone (PCL) and polydiisopropyl fumarate (PDIPF) enriched with 1% or 5% Sr 2+ and evaluated the applicability of these biomaterials for BTE, both in vitro and in vivo. In vitro, whereas Blend + 5% Sr 2+ was pro-inflammatory and anti-osteogenic, Blend + 1% Sr 2+ released very low quantities of the cation; was not cytotoxic for cultured macrophages; and showed improved osteocompatibility when used as a substratum for primary cultures of bone marrow stromal cells. In vivo, implants with Blend + 1% Sr 2+ significantly increased bone tissue regeneration and improved fibrous bridging (vs. Blend alone), while neither inducing a local inflammatory response nor increased serum levels of Sr 2+ . These results indicate that our compatibilized blend of PCL-PDIPF enriched with 1% Sr 2+ could be useful for BTE.Fil: Lino, Agustina Berenice. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: McCarthy, Antonio Desmond. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; ArgentinaFil: Fernández, Juan Manuel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaSpringer2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/131911Lino, Agustina Berenice; McCarthy, Antonio Desmond; Fernández, Juan Manuel; Evaluation of strontium-containing PCL-PDIPF scaffolds for bone tissue engineering: In vitro and in vivo studies; Springer; Annals Of Biomedical Engineering; 47; 3; 12-2018; 902-9120090-6964CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s10439-018-02183-zinfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10439-018-02183-zinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T09:56:35Zoai:ri.conicet.gov.ar:11336/131911instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 09:56:35.85CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of strontium-containing PCL-PDIPF scaffolds for bone tissue engineering: In vitro and in vivo studies
title Evaluation of strontium-containing PCL-PDIPF scaffolds for bone tissue engineering: In vitro and in vivo studies
spellingShingle Evaluation of strontium-containing PCL-PDIPF scaffolds for bone tissue engineering: In vitro and in vivo studies
Lino, Agustina Berenice
BONE MARROW STROMAL CELLS
BONE REGENERATION
POLY-Ε-CAPROLACTONE
POLYDIISOPROPYL FUMARATE
RAW 264.7 MACROPHAGES
STRONTIUM
title_short Evaluation of strontium-containing PCL-PDIPF scaffolds for bone tissue engineering: In vitro and in vivo studies
title_full Evaluation of strontium-containing PCL-PDIPF scaffolds for bone tissue engineering: In vitro and in vivo studies
title_fullStr Evaluation of strontium-containing PCL-PDIPF scaffolds for bone tissue engineering: In vitro and in vivo studies
title_full_unstemmed Evaluation of strontium-containing PCL-PDIPF scaffolds for bone tissue engineering: In vitro and in vivo studies
title_sort Evaluation of strontium-containing PCL-PDIPF scaffolds for bone tissue engineering: In vitro and in vivo studies
dc.creator.none.fl_str_mv Lino, Agustina Berenice
McCarthy, Antonio Desmond
Fernández, Juan Manuel
author Lino, Agustina Berenice
author_facet Lino, Agustina Berenice
McCarthy, Antonio Desmond
Fernández, Juan Manuel
author_role author
author2 McCarthy, Antonio Desmond
Fernández, Juan Manuel
author2_role author
author
dc.subject.none.fl_str_mv BONE MARROW STROMAL CELLS
BONE REGENERATION
POLY-Ε-CAPROLACTONE
POLYDIISOPROPYL FUMARATE
RAW 264.7 MACROPHAGES
STRONTIUM
topic BONE MARROW STROMAL CELLS
BONE REGENERATION
POLY-Ε-CAPROLACTONE
POLYDIISOPROPYL FUMARATE
RAW 264.7 MACROPHAGES
STRONTIUM
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Bone tissue engineering (BTE) has the general objective of restoring and improving damaged bone. A very interesting strategy for BTE is to combine an adequate polymeric scaffold with an osteoinductive compound. Strontium is a divalent cation that can substitute calcium in hydroxyapatite and induce both anabolic and anti-catabolic effects in bone. On the other hand, systemic increases in Sr 2+ levels can provoke adverse cardiovascular effects. In the present study we have developed a compatibilized blend of poly-ε-caprolactone (PCL) and polydiisopropyl fumarate (PDIPF) enriched with 1% or 5% Sr 2+ and evaluated the applicability of these biomaterials for BTE, both in vitro and in vivo. In vitro, whereas Blend + 5% Sr 2+ was pro-inflammatory and anti-osteogenic, Blend + 1% Sr 2+ released very low quantities of the cation; was not cytotoxic for cultured macrophages; and showed improved osteocompatibility when used as a substratum for primary cultures of bone marrow stromal cells. In vivo, implants with Blend + 1% Sr 2+ significantly increased bone tissue regeneration and improved fibrous bridging (vs. Blend alone), while neither inducing a local inflammatory response nor increased serum levels of Sr 2+ . These results indicate that our compatibilized blend of PCL-PDIPF enriched with 1% Sr 2+ could be useful for BTE.
Fil: Lino, Agustina Berenice. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: McCarthy, Antonio Desmond. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina
Fil: Fernández, Juan Manuel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
description Bone tissue engineering (BTE) has the general objective of restoring and improving damaged bone. A very interesting strategy for BTE is to combine an adequate polymeric scaffold with an osteoinductive compound. Strontium is a divalent cation that can substitute calcium in hydroxyapatite and induce both anabolic and anti-catabolic effects in bone. On the other hand, systemic increases in Sr 2+ levels can provoke adverse cardiovascular effects. In the present study we have developed a compatibilized blend of poly-ε-caprolactone (PCL) and polydiisopropyl fumarate (PDIPF) enriched with 1% or 5% Sr 2+ and evaluated the applicability of these biomaterials for BTE, both in vitro and in vivo. In vitro, whereas Blend + 5% Sr 2+ was pro-inflammatory and anti-osteogenic, Blend + 1% Sr 2+ released very low quantities of the cation; was not cytotoxic for cultured macrophages; and showed improved osteocompatibility when used as a substratum for primary cultures of bone marrow stromal cells. In vivo, implants with Blend + 1% Sr 2+ significantly increased bone tissue regeneration and improved fibrous bridging (vs. Blend alone), while neither inducing a local inflammatory response nor increased serum levels of Sr 2+ . These results indicate that our compatibilized blend of PCL-PDIPF enriched with 1% Sr 2+ could be useful for BTE.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/131911
Lino, Agustina Berenice; McCarthy, Antonio Desmond; Fernández, Juan Manuel; Evaluation of strontium-containing PCL-PDIPF scaffolds for bone tissue engineering: In vitro and in vivo studies; Springer; Annals Of Biomedical Engineering; 47; 3; 12-2018; 902-912
0090-6964
CONICET Digital
CONICET
url http://hdl.handle.net/11336/131911
identifier_str_mv Lino, Agustina Berenice; McCarthy, Antonio Desmond; Fernández, Juan Manuel; Evaluation of strontium-containing PCL-PDIPF scaffolds for bone tissue engineering: In vitro and in vivo studies; Springer; Annals Of Biomedical Engineering; 47; 3; 12-2018; 902-912
0090-6964
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s10439-018-02183-z
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10439-018-02183-z
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.087074

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