Fumarate/ceramic composite based Scaffolds for tissue engineering: evaluation of hydrophylicity, degradability, toxicity and biocompatibility

Autores
Fernández, Juan Manuel; Cortizo, María Susana; Cortizo, Ana María
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión enviada
Descripción
The present study was designed to investigate the possible cytotoxicity and biocompatibility of scaffolds based on previously characterized polymeric materials including poly- -caprolactone (PCL) or polydiisopropyl fumarate (blended or on their own), with or without hydroxyapatite (HAP). Water contact angle was also evaluated to determine the hydrophylicity of each scaffold. Degradation of different scaffolds was evaluated after a 10-week incubation in Dulbecco’s modified eagle medium (DMEM) supplemented with 5% (v/v) fetal bovine serum (FBS). Bone Marrow Stromal Cells (MSC) were grown on different scaffolds in an osteogenic medium, after which alkaline phosphatase activity (ALP) was evaluated. ALP activity increased when MSC were grown on PCL+HAP or Blend+HAP, as compared to PCL or Blend without HAP. The effect of different scaffolds on the proliferation of the macrophage cell line RAW 264.7, production of nitric oxide (NO) and secretion of pro-inflammatory cytokines was examined. After 72 h, macrophages proliferated equally well on all scaffolds, maintaining a rounded morphology. None of the investigated scaffolds induced production of NO or cytokine release into the culture media, suggesting an absence of cytotoxicity. Therefore, these polymer- and HAP-based scaffolds could potentially be used as bone substitute materials.
Materia
Ciencias Químicas
cytotoxicity
bone tissue engineering
bone marrow stromal cells
RAW 264.7 macrophages
poly-epsilon-caprolactone
polydiisopropyl fumarate
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/4756

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network_acronym_str CICBA
repository_id_str 9441
network_name_str CIC Digital (CICBA)
spelling Fumarate/ceramic composite based Scaffolds for tissue engineering: evaluation of hydrophylicity, degradability, toxicity and biocompatibilityFernández, Juan ManuelCortizo, María SusanaCortizo, Ana MaríaCiencias Químicascytotoxicitybone tissue engineeringbone marrow stromal cellsRAW 264.7 macrophagespoly-epsilon-caprolactonepolydiisopropyl fumarateThe present study was designed to investigate the possible cytotoxicity and biocompatibility of scaffolds based on previously characterized polymeric materials including poly- -caprolactone (PCL) or polydiisopropyl fumarate (blended or on their own), with or without hydroxyapatite (HAP). Water contact angle was also evaluated to determine the hydrophylicity of each scaffold. Degradation of different scaffolds was evaluated after a 10-week incubation in Dulbecco’s modified eagle medium (DMEM) supplemented with 5% (v/v) fetal bovine serum (FBS). Bone Marrow Stromal Cells (MSC) were grown on different scaffolds in an osteogenic medium, after which alkaline phosphatase activity (ALP) was evaluated. ALP activity increased when MSC were grown on PCL+HAP or Blend+HAP, as compared to PCL or Blend without HAP. The effect of different scaffolds on the proliferation of the macrophage cell line RAW 264.7, production of nitric oxide (NO) and secretion of pro-inflammatory cytokines was examined. After 72 h, macrophages proliferated equally well on all scaffolds, maintaining a rounded morphology. None of the investigated scaffolds induced production of NO or cytokine release into the culture media, suggesting an absence of cytotoxicity. Therefore, these polymer- and HAP-based scaffolds could potentially be used as bone substitute materials.2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/4756enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:40:21Zoai:digital.cic.gba.gob.ar:11746/4756Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:40:21.914CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Fumarate/ceramic composite based Scaffolds for tissue engineering: evaluation of hydrophylicity, degradability, toxicity and biocompatibility
title Fumarate/ceramic composite based Scaffolds for tissue engineering: evaluation of hydrophylicity, degradability, toxicity and biocompatibility
spellingShingle Fumarate/ceramic composite based Scaffolds for tissue engineering: evaluation of hydrophylicity, degradability, toxicity and biocompatibility
Fernández, Juan Manuel
Ciencias Químicas
cytotoxicity
bone tissue engineering
bone marrow stromal cells
RAW 264.7 macrophages
poly-epsilon-caprolactone
polydiisopropyl fumarate
title_short Fumarate/ceramic composite based Scaffolds for tissue engineering: evaluation of hydrophylicity, degradability, toxicity and biocompatibility
title_full Fumarate/ceramic composite based Scaffolds for tissue engineering: evaluation of hydrophylicity, degradability, toxicity and biocompatibility
title_fullStr Fumarate/ceramic composite based Scaffolds for tissue engineering: evaluation of hydrophylicity, degradability, toxicity and biocompatibility
title_full_unstemmed Fumarate/ceramic composite based Scaffolds for tissue engineering: evaluation of hydrophylicity, degradability, toxicity and biocompatibility
title_sort Fumarate/ceramic composite based Scaffolds for tissue engineering: evaluation of hydrophylicity, degradability, toxicity and biocompatibility
dc.creator.none.fl_str_mv Fernández, Juan Manuel
Cortizo, María Susana
Cortizo, Ana María
author Fernández, Juan Manuel
author_facet Fernández, Juan Manuel
Cortizo, María Susana
Cortizo, Ana María
author_role author
author2 Cortizo, María Susana
Cortizo, Ana María
author2_role author
author
dc.subject.none.fl_str_mv Ciencias Químicas
cytotoxicity
bone tissue engineering
bone marrow stromal cells
RAW 264.7 macrophages
poly-epsilon-caprolactone
polydiisopropyl fumarate
topic Ciencias Químicas
cytotoxicity
bone tissue engineering
bone marrow stromal cells
RAW 264.7 macrophages
poly-epsilon-caprolactone
polydiisopropyl fumarate
dc.description.none.fl_txt_mv The present study was designed to investigate the possible cytotoxicity and biocompatibility of scaffolds based on previously characterized polymeric materials including poly- -caprolactone (PCL) or polydiisopropyl fumarate (blended or on their own), with or without hydroxyapatite (HAP). Water contact angle was also evaluated to determine the hydrophylicity of each scaffold. Degradation of different scaffolds was evaluated after a 10-week incubation in Dulbecco’s modified eagle medium (DMEM) supplemented with 5% (v/v) fetal bovine serum (FBS). Bone Marrow Stromal Cells (MSC) were grown on different scaffolds in an osteogenic medium, after which alkaline phosphatase activity (ALP) was evaluated. ALP activity increased when MSC were grown on PCL+HAP or Blend+HAP, as compared to PCL or Blend without HAP. The effect of different scaffolds on the proliferation of the macrophage cell line RAW 264.7, production of nitric oxide (NO) and secretion of pro-inflammatory cytokines was examined. After 72 h, macrophages proliferated equally well on all scaffolds, maintaining a rounded morphology. None of the investigated scaffolds induced production of NO or cytokine release into the culture media, suggesting an absence of cytotoxicity. Therefore, these polymer- and HAP-based scaffolds could potentially be used as bone substitute materials.
description The present study was designed to investigate the possible cytotoxicity and biocompatibility of scaffolds based on previously characterized polymeric materials including poly- -caprolactone (PCL) or polydiisopropyl fumarate (blended or on their own), with or without hydroxyapatite (HAP). Water contact angle was also evaluated to determine the hydrophylicity of each scaffold. Degradation of different scaffolds was evaluated after a 10-week incubation in Dulbecco’s modified eagle medium (DMEM) supplemented with 5% (v/v) fetal bovine serum (FBS). Bone Marrow Stromal Cells (MSC) were grown on different scaffolds in an osteogenic medium, after which alkaline phosphatase activity (ALP) was evaluated. ALP activity increased when MSC were grown on PCL+HAP or Blend+HAP, as compared to PCL or Blend without HAP. The effect of different scaffolds on the proliferation of the macrophage cell line RAW 264.7, production of nitric oxide (NO) and secretion of pro-inflammatory cytokines was examined. After 72 h, macrophages proliferated equally well on all scaffolds, maintaining a rounded morphology. None of the investigated scaffolds induced production of NO or cytokine release into the culture media, suggesting an absence of cytotoxicity. Therefore, these polymer- and HAP-based scaffolds could potentially be used as bone substitute materials.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv https://digital.cic.gba.gob.ar/handle/11746/4756
url https://digital.cic.gba.gob.ar/handle/11746/4756
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:CIC Digital (CICBA)
instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron:CICBA
reponame_str CIC Digital (CICBA)
collection CIC Digital (CICBA)
instname_str Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron_str CICBA
institution CICBA
repository.name.fl_str_mv CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
repository.mail.fl_str_mv marisa.degiusti@sedici.unlp.edu.ar
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