Fumarate/ceramic composite based Scaffolds for tissue engineering: evaluation of hydrophylicity, degradability, toxicity and biocompatibility
- Autores
- Fernández, Juan Manuel; Cortizo, María Susana; Cortizo, Ana María
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión enviada
- Descripción
- The present study was designed to investigate the possible cytotoxicity and biocompatibility of scaffolds based on previously characterized polymeric materials including poly- -caprolactone (PCL) or polydiisopropyl fumarate (blended or on their own), with or without hydroxyapatite (HAP). Water contact angle was also evaluated to determine the hydrophylicity of each scaffold. Degradation of different scaffolds was evaluated after a 10-week incubation in Dulbecco’s modified eagle medium (DMEM) supplemented with 5% (v/v) fetal bovine serum (FBS). Bone Marrow Stromal Cells (MSC) were grown on different scaffolds in an osteogenic medium, after which alkaline phosphatase activity (ALP) was evaluated. ALP activity increased when MSC were grown on PCL+HAP or Blend+HAP, as compared to PCL or Blend without HAP. The effect of different scaffolds on the proliferation of the macrophage cell line RAW 264.7, production of nitric oxide (NO) and secretion of pro-inflammatory cytokines was examined. After 72 h, macrophages proliferated equally well on all scaffolds, maintaining a rounded morphology. None of the investigated scaffolds induced production of NO or cytokine release into the culture media, suggesting an absence of cytotoxicity. Therefore, these polymer- and HAP-based scaffolds could potentially be used as bone substitute materials.
- Materia
-
Ciencias Químicas
cytotoxicity
bone tissue engineering
bone marrow stromal cells
RAW 264.7 macrophages
poly-epsilon-caprolactone
polydiisopropyl fumarate - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
- OAI Identificador
- oai:digital.cic.gba.gob.ar:11746/4756
Ver los metadatos del registro completo
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CIC Digital (CICBA) |
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Fumarate/ceramic composite based Scaffolds for tissue engineering: evaluation of hydrophylicity, degradability, toxicity and biocompatibilityFernández, Juan ManuelCortizo, María SusanaCortizo, Ana MaríaCiencias Químicascytotoxicitybone tissue engineeringbone marrow stromal cellsRAW 264.7 macrophagespoly-epsilon-caprolactonepolydiisopropyl fumarateThe present study was designed to investigate the possible cytotoxicity and biocompatibility of scaffolds based on previously characterized polymeric materials including poly- -caprolactone (PCL) or polydiisopropyl fumarate (blended or on their own), with or without hydroxyapatite (HAP). Water contact angle was also evaluated to determine the hydrophylicity of each scaffold. Degradation of different scaffolds was evaluated after a 10-week incubation in Dulbecco’s modified eagle medium (DMEM) supplemented with 5% (v/v) fetal bovine serum (FBS). Bone Marrow Stromal Cells (MSC) were grown on different scaffolds in an osteogenic medium, after which alkaline phosphatase activity (ALP) was evaluated. ALP activity increased when MSC were grown on PCL+HAP or Blend+HAP, as compared to PCL or Blend without HAP. The effect of different scaffolds on the proliferation of the macrophage cell line RAW 264.7, production of nitric oxide (NO) and secretion of pro-inflammatory cytokines was examined. After 72 h, macrophages proliferated equally well on all scaffolds, maintaining a rounded morphology. None of the investigated scaffolds induced production of NO or cytokine release into the culture media, suggesting an absence of cytotoxicity. Therefore, these polymer- and HAP-based scaffolds could potentially be used as bone substitute materials.2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/4756enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:40:21Zoai:digital.cic.gba.gob.ar:11746/4756Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:40:21.914CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse |
dc.title.none.fl_str_mv |
Fumarate/ceramic composite based Scaffolds for tissue engineering: evaluation of hydrophylicity, degradability, toxicity and biocompatibility |
title |
Fumarate/ceramic composite based Scaffolds for tissue engineering: evaluation of hydrophylicity, degradability, toxicity and biocompatibility |
spellingShingle |
Fumarate/ceramic composite based Scaffolds for tissue engineering: evaluation of hydrophylicity, degradability, toxicity and biocompatibility Fernández, Juan Manuel Ciencias Químicas cytotoxicity bone tissue engineering bone marrow stromal cells RAW 264.7 macrophages poly-epsilon-caprolactone polydiisopropyl fumarate |
title_short |
Fumarate/ceramic composite based Scaffolds for tissue engineering: evaluation of hydrophylicity, degradability, toxicity and biocompatibility |
title_full |
Fumarate/ceramic composite based Scaffolds for tissue engineering: evaluation of hydrophylicity, degradability, toxicity and biocompatibility |
title_fullStr |
Fumarate/ceramic composite based Scaffolds for tissue engineering: evaluation of hydrophylicity, degradability, toxicity and biocompatibility |
title_full_unstemmed |
Fumarate/ceramic composite based Scaffolds for tissue engineering: evaluation of hydrophylicity, degradability, toxicity and biocompatibility |
title_sort |
Fumarate/ceramic composite based Scaffolds for tissue engineering: evaluation of hydrophylicity, degradability, toxicity and biocompatibility |
dc.creator.none.fl_str_mv |
Fernández, Juan Manuel Cortizo, María Susana Cortizo, Ana María |
author |
Fernández, Juan Manuel |
author_facet |
Fernández, Juan Manuel Cortizo, María Susana Cortizo, Ana María |
author_role |
author |
author2 |
Cortizo, María Susana Cortizo, Ana María |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Ciencias Químicas cytotoxicity bone tissue engineering bone marrow stromal cells RAW 264.7 macrophages poly-epsilon-caprolactone polydiisopropyl fumarate |
topic |
Ciencias Químicas cytotoxicity bone tissue engineering bone marrow stromal cells RAW 264.7 macrophages poly-epsilon-caprolactone polydiisopropyl fumarate |
dc.description.none.fl_txt_mv |
The present study was designed to investigate the possible cytotoxicity and biocompatibility of scaffolds based on previously characterized polymeric materials including poly- -caprolactone (PCL) or polydiisopropyl fumarate (blended or on their own), with or without hydroxyapatite (HAP). Water contact angle was also evaluated to determine the hydrophylicity of each scaffold. Degradation of different scaffolds was evaluated after a 10-week incubation in Dulbecco’s modified eagle medium (DMEM) supplemented with 5% (v/v) fetal bovine serum (FBS). Bone Marrow Stromal Cells (MSC) were grown on different scaffolds in an osteogenic medium, after which alkaline phosphatase activity (ALP) was evaluated. ALP activity increased when MSC were grown on PCL+HAP or Blend+HAP, as compared to PCL or Blend without HAP. The effect of different scaffolds on the proliferation of the macrophage cell line RAW 264.7, production of nitric oxide (NO) and secretion of pro-inflammatory cytokines was examined. After 72 h, macrophages proliferated equally well on all scaffolds, maintaining a rounded morphology. None of the investigated scaffolds induced production of NO or cytokine release into the culture media, suggesting an absence of cytotoxicity. Therefore, these polymer- and HAP-based scaffolds could potentially be used as bone substitute materials. |
description |
The present study was designed to investigate the possible cytotoxicity and biocompatibility of scaffolds based on previously characterized polymeric materials including poly- -caprolactone (PCL) or polydiisopropyl fumarate (blended or on their own), with or without hydroxyapatite (HAP). Water contact angle was also evaluated to determine the hydrophylicity of each scaffold. Degradation of different scaffolds was evaluated after a 10-week incubation in Dulbecco’s modified eagle medium (DMEM) supplemented with 5% (v/v) fetal bovine serum (FBS). Bone Marrow Stromal Cells (MSC) were grown on different scaffolds in an osteogenic medium, after which alkaline phosphatase activity (ALP) was evaluated. ALP activity increased when MSC were grown on PCL+HAP or Blend+HAP, as compared to PCL or Blend without HAP. The effect of different scaffolds on the proliferation of the macrophage cell line RAW 264.7, production of nitric oxide (NO) and secretion of pro-inflammatory cytokines was examined. After 72 h, macrophages proliferated equally well on all scaffolds, maintaining a rounded morphology. None of the investigated scaffolds induced production of NO or cytokine release into the culture media, suggesting an absence of cytotoxicity. Therefore, these polymer- and HAP-based scaffolds could potentially be used as bone substitute materials. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/submittedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
submittedVersion |
dc.identifier.none.fl_str_mv |
https://digital.cic.gba.gob.ar/handle/11746/4756 |
url |
https://digital.cic.gba.gob.ar/handle/11746/4756 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:CIC Digital (CICBA) instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires instacron:CICBA |
reponame_str |
CIC Digital (CICBA) |
collection |
CIC Digital (CICBA) |
instname_str |
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
instacron_str |
CICBA |
institution |
CICBA |
repository.name.fl_str_mv |
CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
repository.mail.fl_str_mv |
marisa.degiusti@sedici.unlp.edu.ar |
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1844618619551481856 |
score |
13.070432 |