Fumarate/Ceramic Composite Based Scaffolds for Tissue Engineering: Evaluation of Hydrophylicity, Degradability, Toxicity and Biocompatibility

Autores
Fernández, Juan Manuel; Cortizo, Maria Susana; Cortizo, Ana María
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The present study was designed to investigate the possible cytotoxicity and biocompatibility of scaffolds based on previously characterized polymeric materials including poly-ε-caprolactone (PCL) or polydiisopropyl fumarate (blended or on their own), with or without hydroxyapatite (HAP). Water contact angle was also evaluated to determine the hydrophylicity of each scaffold. Degradation of different scaffolds was evaluated after a 10-week incubation in Dulbecco's modified eagle medium (DMEM) supplemented with 5% (v/v) fetal bovine serum (FBS). Bone Marrow Stromal Cells (MSC) were grown on different scaffolds in an osteogenic medium, after which alkaline phosphatase activity (ALP) was evaluated. ALP activity increased when MSC were grown on PCL + HAP or Blend + HAP, as compared to PCL or Blend without HAP. The effect of different scaffolds on the proliferation of the macrophage cell line RAW 264.7, production of nitric oxide (NO) and secretion of pro-inflammatory cytokines was examined. After 72 h, macrophages proliferated equally well on all scaffolds, maintaining a rounded morphology. None of the investigated scaffolds induced production of NO or cytokine release into the culture media, suggesting an absence of cytotoxicity. Therefore, these polymer- and HAP-based scaffolds could potentially be used as bone substitute materials.
Fil: Fernández, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina
Fil: Cortizo, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Cortizo, Ana María. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina
Materia
Cytotoxicity
Bone Tissue Engineering
Bone Marrow Stromal Cell
Polydiisopropyl Fumarate
Poly-Epsilon-Caprolactone
Fumarate
Raw 264.7 Macrophages
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/32946
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/32946
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Fumarate/Ceramic Composite Based Scaffolds for Tissue Engineering: Evaluation of Hydrophylicity, Degradability, Toxicity and BiocompatibilityFernández, Juan ManuelCortizo, Maria SusanaCortizo, Ana MaríaCytotoxicityBone Tissue EngineeringBone Marrow Stromal CellPolydiisopropyl FumaratePoly-Epsilon-CaprolactoneFumarateRaw 264.7 Macrophageshttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3The present study was designed to investigate the possible cytotoxicity and biocompatibility of scaffolds based on previously characterized polymeric materials including poly-ε-caprolactone (PCL) or polydiisopropyl fumarate (blended or on their own), with or without hydroxyapatite (HAP). Water contact angle was also evaluated to determine the hydrophylicity of each scaffold. Degradation of different scaffolds was evaluated after a 10-week incubation in Dulbecco's modified eagle medium (DMEM) supplemented with 5% (v/v) fetal bovine serum (FBS). Bone Marrow Stromal Cells (MSC) were grown on different scaffolds in an osteogenic medium, after which alkaline phosphatase activity (ALP) was evaluated. ALP activity increased when MSC were grown on PCL + HAP or Blend + HAP, as compared to PCL or Blend without HAP. The effect of different scaffolds on the proliferation of the macrophage cell line RAW 264.7, production of nitric oxide (NO) and secretion of pro-inflammatory cytokines was examined. After 72 h, macrophages proliferated equally well on all scaffolds, maintaining a rounded morphology. None of the investigated scaffolds induced production of NO or cytokine release into the culture media, suggesting an absence of cytotoxicity. Therefore, these polymer- and HAP-based scaffolds could potentially be used as bone substitute materials.Fil: Fernández, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; ArgentinaFil: Cortizo, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Cortizo, Ana María. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; ArgentinaAmerican Scientific Publishers2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32946Cortizo, Ana María; Cortizo, Maria Susana; Fernández, Juan Manuel; Fumarate/Ceramic Composite Based Scaffolds for Tissue Engineering: Evaluation of Hydrophylicity, Degradability, Toxicity and Biocompatibility; American Scientific Publishers; Journal of Biomaterials and Tissue Engineering; 4; 3; 3-2014; 227-2342157-9083CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/content/asp/jbte/2014/00000004/00000003/art00010info:eu-repo/semantics/altIdentifier/doi/10.1166/jbt.2014.1158info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:27:09Zoai:ri.conicet.gov.ar:11336/32946instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:27:10.079CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fumarate/Ceramic Composite Based Scaffolds for Tissue Engineering: Evaluation of Hydrophylicity, Degradability, Toxicity and Biocompatibility
title Fumarate/Ceramic Composite Based Scaffolds for Tissue Engineering: Evaluation of Hydrophylicity, Degradability, Toxicity and Biocompatibility
spellingShingle Fumarate/Ceramic Composite Based Scaffolds for Tissue Engineering: Evaluation of Hydrophylicity, Degradability, Toxicity and Biocompatibility
Fernández, Juan Manuel
Cytotoxicity
Bone Tissue Engineering
Bone Marrow Stromal Cell
Polydiisopropyl Fumarate
Poly-Epsilon-Caprolactone
Fumarate
Raw 264.7 Macrophages
title_short Fumarate/Ceramic Composite Based Scaffolds for Tissue Engineering: Evaluation of Hydrophylicity, Degradability, Toxicity and Biocompatibility
title_full Fumarate/Ceramic Composite Based Scaffolds for Tissue Engineering: Evaluation of Hydrophylicity, Degradability, Toxicity and Biocompatibility
title_fullStr Fumarate/Ceramic Composite Based Scaffolds for Tissue Engineering: Evaluation of Hydrophylicity, Degradability, Toxicity and Biocompatibility
title_full_unstemmed Fumarate/Ceramic Composite Based Scaffolds for Tissue Engineering: Evaluation of Hydrophylicity, Degradability, Toxicity and Biocompatibility
title_sort Fumarate/Ceramic Composite Based Scaffolds for Tissue Engineering: Evaluation of Hydrophylicity, Degradability, Toxicity and Biocompatibility
dc.creator.none.fl_str_mv Fernández, Juan Manuel
Cortizo, Maria Susana
Cortizo, Ana María
author Fernández, Juan Manuel
author_facet Fernández, Juan Manuel
Cortizo, Maria Susana
Cortizo, Ana María
author_role author
author2 Cortizo, Maria Susana
Cortizo, Ana María
author2_role author
author
dc.subject.none.fl_str_mv Cytotoxicity
Bone Tissue Engineering
Bone Marrow Stromal Cell
Polydiisopropyl Fumarate
Poly-Epsilon-Caprolactone
Fumarate
Raw 264.7 Macrophages
topic Cytotoxicity
Bone Tissue Engineering
Bone Marrow Stromal Cell
Polydiisopropyl Fumarate
Poly-Epsilon-Caprolactone
Fumarate
Raw 264.7 Macrophages
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The present study was designed to investigate the possible cytotoxicity and biocompatibility of scaffolds based on previously characterized polymeric materials including poly-ε-caprolactone (PCL) or polydiisopropyl fumarate (blended or on their own), with or without hydroxyapatite (HAP). Water contact angle was also evaluated to determine the hydrophylicity of each scaffold. Degradation of different scaffolds was evaluated after a 10-week incubation in Dulbecco's modified eagle medium (DMEM) supplemented with 5% (v/v) fetal bovine serum (FBS). Bone Marrow Stromal Cells (MSC) were grown on different scaffolds in an osteogenic medium, after which alkaline phosphatase activity (ALP) was evaluated. ALP activity increased when MSC were grown on PCL + HAP or Blend + HAP, as compared to PCL or Blend without HAP. The effect of different scaffolds on the proliferation of the macrophage cell line RAW 264.7, production of nitric oxide (NO) and secretion of pro-inflammatory cytokines was examined. After 72 h, macrophages proliferated equally well on all scaffolds, maintaining a rounded morphology. None of the investigated scaffolds induced production of NO or cytokine release into the culture media, suggesting an absence of cytotoxicity. Therefore, these polymer- and HAP-based scaffolds could potentially be used as bone substitute materials.
Fil: Fernández, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina
Fil: Cortizo, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Cortizo, Ana María. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina
description The present study was designed to investigate the possible cytotoxicity and biocompatibility of scaffolds based on previously characterized polymeric materials including poly-ε-caprolactone (PCL) or polydiisopropyl fumarate (blended or on their own), with or without hydroxyapatite (HAP). Water contact angle was also evaluated to determine the hydrophylicity of each scaffold. Degradation of different scaffolds was evaluated after a 10-week incubation in Dulbecco's modified eagle medium (DMEM) supplemented with 5% (v/v) fetal bovine serum (FBS). Bone Marrow Stromal Cells (MSC) were grown on different scaffolds in an osteogenic medium, after which alkaline phosphatase activity (ALP) was evaluated. ALP activity increased when MSC were grown on PCL + HAP or Blend + HAP, as compared to PCL or Blend without HAP. The effect of different scaffolds on the proliferation of the macrophage cell line RAW 264.7, production of nitric oxide (NO) and secretion of pro-inflammatory cytokines was examined. After 72 h, macrophages proliferated equally well on all scaffolds, maintaining a rounded morphology. None of the investigated scaffolds induced production of NO or cytokine release into the culture media, suggesting an absence of cytotoxicity. Therefore, these polymer- and HAP-based scaffolds could potentially be used as bone substitute materials.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/32946
Cortizo, Ana María; Cortizo, Maria Susana; Fernández, Juan Manuel; Fumarate/Ceramic Composite Based Scaffolds for Tissue Engineering: Evaluation of Hydrophylicity, Degradability, Toxicity and Biocompatibility; American Scientific Publishers; Journal of Biomaterials and Tissue Engineering; 4; 3; 3-2014; 227-234
2157-9083
CONICET Digital
CONICET
url http://hdl.handle.net/11336/32946
identifier_str_mv Cortizo, Ana María; Cortizo, Maria Susana; Fernández, Juan Manuel; Fumarate/Ceramic Composite Based Scaffolds for Tissue Engineering: Evaluation of Hydrophylicity, Degradability, Toxicity and Biocompatibility; American Scientific Publishers; Journal of Biomaterials and Tissue Engineering; 4; 3; 3-2014; 227-234
2157-9083
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/content/asp/jbte/2014/00000004/00000003/art00010
info:eu-repo/semantics/altIdentifier/doi/10.1166/jbt.2014.1158
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Scientific Publishers
publisher.none.fl_str_mv American Scientific Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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