Varicella zoster virus ORF25 gene product: an essential hub protein linking encapsidation proteins and the nuclear egress complex.

Autores
Vizoso Pinto, María Guadalupe; Pothineni, Venkata R.; Haase, Rudolf; Woidy, Mathias; Lotz Havla, Amelie; Gersting, Soren W.; Muntau, Ania C.; Haas, Jurgen; Sommer, Marvin; Arvin, Ann M.; Baiker, Armin
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Varicella zoster virus (VZV) ORF25 is a 156 amino acid protein belonging to the approximately 40 core proteins that are conserved throughout the Herpesviridae. By analogy to its functional orthologue UL33 in Herpes simplex virus 1 (HSV-1), ORF25 is thought to be a component of the terminase complex. To investigate how cleavage and encapsidation of viral DNA links to the nuclear egress of mature capsids in VZV, we tested 10 VZV proteins that are predicted to be involved in either of the two processes for protein interactions against each other using three independent protein-protein interaction (PPI) detection systems: the yeast-two-hybrid (Y2H) system, a luminescence based MBP pull-down interaction screening assay (LuMPIS), and a bioluminescence resonance energy transfer (BRET) assay. A set of 20 interactions was consistently detected by at least 2 methods and resulted in a dense interaction network between proteins associated in encapsidation and nuclear egress. The results indicate that the terminase complex in VZV consists of ORF25, ORF30, and ORF45/42 and support a model in which both processes are closely linked to each other. Consistent with its role as a central hub for protein interactions, ORF25 is shown to be essential for VZV replication.
Fil: Vizoso Pinto, María Guadalupe. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute. Cátedra Virology; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Pothineni, Venkata R.. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute. Cátedra Virology; Alemania
Fil: Haase, Rudolf. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute. Cátedra Virology; Alemania
Fil: Woidy, Mathias. Ludwig Maximilians Universitat; Alemania
Fil: Lotz Havla, Amelie. Ludwig Maximilians Universitat; Alemania
Fil: Gersting, Soren W.. Ludwig Maximilians Universitat; Alemania
Fil: Muntau, Ania C.. Ludwig Maximilians Universitat; Alemania
Fil: Haas, Jurgen. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute. Cátedra Virology; Alemania
Fil: Sommer, Marvin. University of Stanford; Estados Unidos
Fil: Arvin, Ann M.. University of Stanford; Estados Unidos
Fil: Baiker, Armin. Bavarian Health and Food Safety Authority; Alemania
Materia
Lumpis
Bret
Y2h
Orf25
Varicella Zoster
Proteome
Protein-Protein Interactions
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/77519
Acceder
id CONICETDig_bc99b069bff8b0e09f550c61ea136e29
oai_identifier_str oai:ri.conicet.gov.ar:11336/77519
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Varicella zoster virus ORF25 gene product: an essential hub protein linking encapsidation proteins and the nuclear egress complex.Vizoso Pinto, María GuadalupePothineni, Venkata R.Haase, RudolfWoidy, MathiasLotz Havla, AmelieGersting, Soren W.Muntau, Ania C.Haas, JurgenSommer, MarvinArvin, Ann M.Baiker, ArminLumpisBretY2hOrf25Varicella ZosterProteomeProtein-Protein Interactionshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Varicella zoster virus (VZV) ORF25 is a 156 amino acid protein belonging to the approximately 40 core proteins that are conserved throughout the Herpesviridae. By analogy to its functional orthologue UL33 in Herpes simplex virus 1 (HSV-1), ORF25 is thought to be a component of the terminase complex. To investigate how cleavage and encapsidation of viral DNA links to the nuclear egress of mature capsids in VZV, we tested 10 VZV proteins that are predicted to be involved in either of the two processes for protein interactions against each other using three independent protein-protein interaction (PPI) detection systems: the yeast-two-hybrid (Y2H) system, a luminescence based MBP pull-down interaction screening assay (LuMPIS), and a bioluminescence resonance energy transfer (BRET) assay. A set of 20 interactions was consistently detected by at least 2 methods and resulted in a dense interaction network between proteins associated in encapsidation and nuclear egress. The results indicate that the terminase complex in VZV consists of ORF25, ORF30, and ORF45/42 and support a model in which both processes are closely linked to each other. Consistent with its role as a central hub for protein interactions, ORF25 is shown to be essential for VZV replication.Fil: Vizoso Pinto, María Guadalupe. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute. Cátedra Virology; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Pothineni, Venkata R.. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute. Cátedra Virology; AlemaniaFil: Haase, Rudolf. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute. Cátedra Virology; AlemaniaFil: Woidy, Mathias. Ludwig Maximilians Universitat; AlemaniaFil: Lotz Havla, Amelie. Ludwig Maximilians Universitat; AlemaniaFil: Gersting, Soren W.. Ludwig Maximilians Universitat; AlemaniaFil: Muntau, Ania C.. Ludwig Maximilians Universitat; AlemaniaFil: Haas, Jurgen. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute. Cátedra Virology; AlemaniaFil: Sommer, Marvin. University of Stanford; Estados UnidosFil: Arvin, Ann M.. University of Stanford; Estados UnidosFil: Baiker, Armin. Bavarian Health and Food Safety Authority; AlemaniaAmerican Chemical Society2011-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/77519Vizoso Pinto, María Guadalupe; Pothineni, Venkata R.; Haase, Rudolf; Woidy, Mathias; Lotz Havla, Amelie; et al.; Varicella zoster virus ORF25 gene product: an essential hub protein linking encapsidation proteins and the nuclear egress complex.; American Chemical Society; Journal of Proteome Research; 10; 12; 12-2011; 5374-53821535-3893CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/pr200628sinfo:eu-repo/semantics/altIdentifier/doi/10.1021/pr200628sinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-26T08:58:04Zoai:ri.conicet.gov.ar:11336/77519instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-26 08:58:04.373CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Varicella zoster virus ORF25 gene product: an essential hub protein linking encapsidation proteins and the nuclear egress complex.
title Varicella zoster virus ORF25 gene product: an essential hub protein linking encapsidation proteins and the nuclear egress complex.
spellingShingle Varicella zoster virus ORF25 gene product: an essential hub protein linking encapsidation proteins and the nuclear egress complex.
Vizoso Pinto, María Guadalupe
Lumpis
Bret
Y2h
Orf25
Varicella Zoster
Proteome
Protein-Protein Interactions
title_short Varicella zoster virus ORF25 gene product: an essential hub protein linking encapsidation proteins and the nuclear egress complex.
title_full Varicella zoster virus ORF25 gene product: an essential hub protein linking encapsidation proteins and the nuclear egress complex.
title_fullStr Varicella zoster virus ORF25 gene product: an essential hub protein linking encapsidation proteins and the nuclear egress complex.
title_full_unstemmed Varicella zoster virus ORF25 gene product: an essential hub protein linking encapsidation proteins and the nuclear egress complex.
title_sort Varicella zoster virus ORF25 gene product: an essential hub protein linking encapsidation proteins and the nuclear egress complex.
dc.creator.none.fl_str_mv Vizoso Pinto, María Guadalupe
Pothineni, Venkata R.
Haase, Rudolf
Woidy, Mathias
Lotz Havla, Amelie
Gersting, Soren W.
Muntau, Ania C.
Haas, Jurgen
Sommer, Marvin
Arvin, Ann M.
Baiker, Armin
author Vizoso Pinto, María Guadalupe
author_facet Vizoso Pinto, María Guadalupe
Pothineni, Venkata R.
Haase, Rudolf
Woidy, Mathias
Lotz Havla, Amelie
Gersting, Soren W.
Muntau, Ania C.
Haas, Jurgen
Sommer, Marvin
Arvin, Ann M.
Baiker, Armin
author_role author
author2 Pothineni, Venkata R.
Haase, Rudolf
Woidy, Mathias
Lotz Havla, Amelie
Gersting, Soren W.
Muntau, Ania C.
Haas, Jurgen
Sommer, Marvin
Arvin, Ann M.
Baiker, Armin
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Lumpis
Bret
Y2h
Orf25
Varicella Zoster
Proteome
Protein-Protein Interactions
topic Lumpis
Bret
Y2h
Orf25
Varicella Zoster
Proteome
Protein-Protein Interactions
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Varicella zoster virus (VZV) ORF25 is a 156 amino acid protein belonging to the approximately 40 core proteins that are conserved throughout the Herpesviridae. By analogy to its functional orthologue UL33 in Herpes simplex virus 1 (HSV-1), ORF25 is thought to be a component of the terminase complex. To investigate how cleavage and encapsidation of viral DNA links to the nuclear egress of mature capsids in VZV, we tested 10 VZV proteins that are predicted to be involved in either of the two processes for protein interactions against each other using three independent protein-protein interaction (PPI) detection systems: the yeast-two-hybrid (Y2H) system, a luminescence based MBP pull-down interaction screening assay (LuMPIS), and a bioluminescence resonance energy transfer (BRET) assay. A set of 20 interactions was consistently detected by at least 2 methods and resulted in a dense interaction network between proteins associated in encapsidation and nuclear egress. The results indicate that the terminase complex in VZV consists of ORF25, ORF30, and ORF45/42 and support a model in which both processes are closely linked to each other. Consistent with its role as a central hub for protein interactions, ORF25 is shown to be essential for VZV replication.
Fil: Vizoso Pinto, María Guadalupe. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute. Cátedra Virology; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Pothineni, Venkata R.. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute. Cátedra Virology; Alemania
Fil: Haase, Rudolf. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute. Cátedra Virology; Alemania
Fil: Woidy, Mathias. Ludwig Maximilians Universitat; Alemania
Fil: Lotz Havla, Amelie. Ludwig Maximilians Universitat; Alemania
Fil: Gersting, Soren W.. Ludwig Maximilians Universitat; Alemania
Fil: Muntau, Ania C.. Ludwig Maximilians Universitat; Alemania
Fil: Haas, Jurgen. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute. Cátedra Virology; Alemania
Fil: Sommer, Marvin. University of Stanford; Estados Unidos
Fil: Arvin, Ann M.. University of Stanford; Estados Unidos
Fil: Baiker, Armin. Bavarian Health and Food Safety Authority; Alemania
description Varicella zoster virus (VZV) ORF25 is a 156 amino acid protein belonging to the approximately 40 core proteins that are conserved throughout the Herpesviridae. By analogy to its functional orthologue UL33 in Herpes simplex virus 1 (HSV-1), ORF25 is thought to be a component of the terminase complex. To investigate how cleavage and encapsidation of viral DNA links to the nuclear egress of mature capsids in VZV, we tested 10 VZV proteins that are predicted to be involved in either of the two processes for protein interactions against each other using three independent protein-protein interaction (PPI) detection systems: the yeast-two-hybrid (Y2H) system, a luminescence based MBP pull-down interaction screening assay (LuMPIS), and a bioluminescence resonance energy transfer (BRET) assay. A set of 20 interactions was consistently detected by at least 2 methods and resulted in a dense interaction network between proteins associated in encapsidation and nuclear egress. The results indicate that the terminase complex in VZV consists of ORF25, ORF30, and ORF45/42 and support a model in which both processes are closely linked to each other. Consistent with its role as a central hub for protein interactions, ORF25 is shown to be essential for VZV replication.
publishDate 2011
dc.date.none.fl_str_mv 2011-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/77519
Vizoso Pinto, María Guadalupe; Pothineni, Venkata R.; Haase, Rudolf; Woidy, Mathias; Lotz Havla, Amelie; et al.; Varicella zoster virus ORF25 gene product: an essential hub protein linking encapsidation proteins and the nuclear egress complex.; American Chemical Society; Journal of Proteome Research; 10; 12; 12-2011; 5374-5382
1535-3893
CONICET Digital
CONICET
url http://hdl.handle.net/11336/77519
identifier_str_mv Vizoso Pinto, María Guadalupe; Pothineni, Venkata R.; Haase, Rudolf; Woidy, Mathias; Lotz Havla, Amelie; et al.; Varicella zoster virus ORF25 gene product: an essential hub protein linking encapsidation proteins and the nuclear egress complex.; American Chemical Society; Journal of Proteome Research; 10; 12; 12-2011; 5374-5382
1535-3893
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/pr200628s
info:eu-repo/semantics/altIdentifier/doi/10.1021/pr200628s
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1849873235954368512
score 13.011256

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