Anti-Candida activity of beneficial vaginal lactobacilli in in vitro assays and in a murine experimental model

Autores
de Gregorio, Priscilla Romina; Silva, Jessica Alejandra; Marchesi, Antonella; Nader, Maria Elena Fatima
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Vulvovaginal candidiasis (VVC) is one of the most frequent infections affecting women worldwide. Healthy vaginalmicrobiota is dominated by lactobacilli, which form a strong defense line against pathogens. In this work, in vitroantimicrobial properties of thirty vaginal Lactobacillus strains were evaluated against eleven Candida vaginal clinical isolates,employing three different methods. Also, the effect of intravaginal (i.va.) administrations (preventive, therapeutic andpreventive-therapeutic) of L. reuteri CRL1324 or L. rhamnosus CRL1332 strains against the i.va. challenge with Candida albicansC2 (C.a.) was evaluated in a murine experimental model. From the results of agar overlay and liquid medium assays theselected lactobacilli strains have shown to inhibit the growth of at least one Candida strain. The inhibition was mainly dueto the effect of organic acids. Anti-Candida activity was not evidenced in the agar plate diffusion method. In theexperimental murine model, only preventive-therapeutic administration of both lactobacilli was able to significantly reduceviable C.a. numbers recovered in vaginal washes and the leukocyte influx induced by the fungi. In conclusion, lactobacilliexhibited in vitro and in vivo antimicrobial effects on Candida, suggesting that they could be promising candidates forprotection against VVC. Lactobacilli predominant in human vagina and protect from VVC. The manuscript reports different methods to determine lactobacilli inhibitory effect at the laboratory, and protection against Candida in murine vagina.
Fil: de Gregorio, Priscilla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Silva, Jessica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Marchesi, Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Materia
CANDIDA SPP
BENEFICIAL LACTOBACILLI
MURINE MODEL
VULVOVAGINAL CANDIDIASIS
IN VITRO AND IN VIVO STUDIES
ANTI-CANDIDA ACTIVITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/121552
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/121552
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Anti-Candida activity of beneficial vaginal lactobacilli in in vitro assays and in a murine experimental modelde Gregorio, Priscilla RominaSilva, Jessica AlejandraMarchesi, AntonellaNader, Maria Elena FatimaCANDIDA SPPBENEFICIAL LACTOBACILLIMURINE MODELVULVOVAGINAL CANDIDIASISIN VITRO AND IN VIVO STUDIESANTI-CANDIDA ACTIVITYhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Vulvovaginal candidiasis (VVC) is one of the most frequent infections affecting women worldwide. Healthy vaginalmicrobiota is dominated by lactobacilli, which form a strong defense line against pathogens. In this work, in vitroantimicrobial properties of thirty vaginal Lactobacillus strains were evaluated against eleven Candida vaginal clinical isolates,employing three different methods. Also, the effect of intravaginal (i.va.) administrations (preventive, therapeutic andpreventive-therapeutic) of L. reuteri CRL1324 or L. rhamnosus CRL1332 strains against the i.va. challenge with Candida albicansC2 (C.a.) was evaluated in a murine experimental model. From the results of agar overlay and liquid medium assays theselected lactobacilli strains have shown to inhibit the growth of at least one Candida strain. The inhibition was mainly dueto the effect of organic acids. Anti-Candida activity was not evidenced in the agar plate diffusion method. In theexperimental murine model, only preventive-therapeutic administration of both lactobacilli was able to significantly reduceviable C.a. numbers recovered in vaginal washes and the leukocyte influx induced by the fungi. In conclusion, lactobacilliexhibited in vitro and in vivo antimicrobial effects on Candida, suggesting that they could be promising candidates forprotection against VVC. Lactobacilli predominant in human vagina and protect from VVC. The manuscript reports different methods to determine lactobacilli inhibitory effect at the laboratory, and protection against Candida in murine vagina.Fil: de Gregorio, Priscilla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Silva, Jessica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Marchesi, Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaOxford University Press2019-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/121552de Gregorio, Priscilla Romina; Silva, Jessica Alejandra; Marchesi, Antonella; Nader, Maria Elena Fatima; Anti-Candida activity of beneficial vaginal lactobacilli in in vitro assays and in a murine experimental model; Oxford University Press; Fems Yeast Research; 19; 2; 3-2019; 1-14; foz0081567-13561567-1364CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/femsyr/foz008info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsyr/article/19/2/foz008/5300136info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:05:36Zoai:ri.conicet.gov.ar:11336/121552instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:05:37.196CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Anti-Candida activity of beneficial vaginal lactobacilli in in vitro assays and in a murine experimental model
title Anti-Candida activity of beneficial vaginal lactobacilli in in vitro assays and in a murine experimental model
spellingShingle Anti-Candida activity of beneficial vaginal lactobacilli in in vitro assays and in a murine experimental model
de Gregorio, Priscilla Romina
CANDIDA SPP
BENEFICIAL LACTOBACILLI
MURINE MODEL
VULVOVAGINAL CANDIDIASIS
IN VITRO AND IN VIVO STUDIES
ANTI-CANDIDA ACTIVITY
title_short Anti-Candida activity of beneficial vaginal lactobacilli in in vitro assays and in a murine experimental model
title_full Anti-Candida activity of beneficial vaginal lactobacilli in in vitro assays and in a murine experimental model
title_fullStr Anti-Candida activity of beneficial vaginal lactobacilli in in vitro assays and in a murine experimental model
title_full_unstemmed Anti-Candida activity of beneficial vaginal lactobacilli in in vitro assays and in a murine experimental model
title_sort Anti-Candida activity of beneficial vaginal lactobacilli in in vitro assays and in a murine experimental model
dc.creator.none.fl_str_mv de Gregorio, Priscilla Romina
Silva, Jessica Alejandra
Marchesi, Antonella
Nader, Maria Elena Fatima
author de Gregorio, Priscilla Romina
author_facet de Gregorio, Priscilla Romina
Silva, Jessica Alejandra
Marchesi, Antonella
Nader, Maria Elena Fatima
author_role author
author2 Silva, Jessica Alejandra
Marchesi, Antonella
Nader, Maria Elena Fatima
author2_role author
author
author
dc.subject.none.fl_str_mv CANDIDA SPP
BENEFICIAL LACTOBACILLI
MURINE MODEL
VULVOVAGINAL CANDIDIASIS
IN VITRO AND IN VIVO STUDIES
ANTI-CANDIDA ACTIVITY
topic CANDIDA SPP
BENEFICIAL LACTOBACILLI
MURINE MODEL
VULVOVAGINAL CANDIDIASIS
IN VITRO AND IN VIVO STUDIES
ANTI-CANDIDA ACTIVITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Vulvovaginal candidiasis (VVC) is one of the most frequent infections affecting women worldwide. Healthy vaginalmicrobiota is dominated by lactobacilli, which form a strong defense line against pathogens. In this work, in vitroantimicrobial properties of thirty vaginal Lactobacillus strains were evaluated against eleven Candida vaginal clinical isolates,employing three different methods. Also, the effect of intravaginal (i.va.) administrations (preventive, therapeutic andpreventive-therapeutic) of L. reuteri CRL1324 or L. rhamnosus CRL1332 strains against the i.va. challenge with Candida albicansC2 (C.a.) was evaluated in a murine experimental model. From the results of agar overlay and liquid medium assays theselected lactobacilli strains have shown to inhibit the growth of at least one Candida strain. The inhibition was mainly dueto the effect of organic acids. Anti-Candida activity was not evidenced in the agar plate diffusion method. In theexperimental murine model, only preventive-therapeutic administration of both lactobacilli was able to significantly reduceviable C.a. numbers recovered in vaginal washes and the leukocyte influx induced by the fungi. In conclusion, lactobacilliexhibited in vitro and in vivo antimicrobial effects on Candida, suggesting that they could be promising candidates forprotection against VVC. Lactobacilli predominant in human vagina and protect from VVC. The manuscript reports different methods to determine lactobacilli inhibitory effect at the laboratory, and protection against Candida in murine vagina.
Fil: de Gregorio, Priscilla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Silva, Jessica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Marchesi, Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
description Vulvovaginal candidiasis (VVC) is one of the most frequent infections affecting women worldwide. Healthy vaginalmicrobiota is dominated by lactobacilli, which form a strong defense line against pathogens. In this work, in vitroantimicrobial properties of thirty vaginal Lactobacillus strains were evaluated against eleven Candida vaginal clinical isolates,employing three different methods. Also, the effect of intravaginal (i.va.) administrations (preventive, therapeutic andpreventive-therapeutic) of L. reuteri CRL1324 or L. rhamnosus CRL1332 strains against the i.va. challenge with Candida albicansC2 (C.a.) was evaluated in a murine experimental model. From the results of agar overlay and liquid medium assays theselected lactobacilli strains have shown to inhibit the growth of at least one Candida strain. The inhibition was mainly dueto the effect of organic acids. Anti-Candida activity was not evidenced in the agar plate diffusion method. In theexperimental murine model, only preventive-therapeutic administration of both lactobacilli was able to significantly reduceviable C.a. numbers recovered in vaginal washes and the leukocyte influx induced by the fungi. In conclusion, lactobacilliexhibited in vitro and in vivo antimicrobial effects on Candida, suggesting that they could be promising candidates forprotection against VVC. Lactobacilli predominant in human vagina and protect from VVC. The manuscript reports different methods to determine lactobacilli inhibitory effect at the laboratory, and protection against Candida in murine vagina.
publishDate 2019
dc.date.none.fl_str_mv 2019-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/121552
de Gregorio, Priscilla Romina; Silva, Jessica Alejandra; Marchesi, Antonella; Nader, Maria Elena Fatima; Anti-Candida activity of beneficial vaginal lactobacilli in in vitro assays and in a murine experimental model; Oxford University Press; Fems Yeast Research; 19; 2; 3-2019; 1-14; foz008
1567-1356
1567-1364
CONICET Digital
CONICET
url http://hdl.handle.net/11336/121552
identifier_str_mv de Gregorio, Priscilla Romina; Silva, Jessica Alejandra; Marchesi, Antonella; Nader, Maria Elena Fatima; Anti-Candida activity of beneficial vaginal lactobacilli in in vitro assays and in a murine experimental model; Oxford University Press; Fems Yeast Research; 19; 2; 3-2019; 1-14; foz008
1567-1356
1567-1364
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/femsyr/foz008
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsyr/article/19/2/foz008/5300136
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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