Intestinal populations of Lactobacilli and coliforms after in vivo Salmonella dublin challenge and their relationship with microbial translocation in calves supplemented with lacti...

Autores
Frizzo, Laureano Sebastian; Soto, Lorena Paola; Bertozzi, Ezequiel; Zbrun, María Virginia; Signorini Porchietto, Marcelo Lisandro; Sequeira, Gabriel Jorge; Rodriguez Armesto, Roberto; Rosmini, Marcelo Raul
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the present study was to evaluate the relationship between intestinal microbial populations and bacterial translocation in young calves supplemented with lactic acid bacteria (LAB) and lactose and experimentally infected with Salmonella dublin DSPV 595T. Fifteen calves were divided into three groups (a control group, a group inoculated with LAB, and a group inoculated with LAB and lactose), with 5, 6 and 4 animals each, were used. The LAB inoculum, composed of Lactobacillus casei DSPV 318T, L. salivarius DSPV 315T and Pediococcus acidilactici DSPV 006T was administered together with the milk replacer. The groups inoculated with LAB and LAB and lactose received a daily dose of 109CFU/kg body weight of each strain throughout the experiment. Lactose was provided in doses of 100g/d. The administration of the pathogen was performed on all animals on day 11 of the experiment with 2×1010CFU. The levels of probiotic inoculum found in the large intestine of the animals from the two groups inoculated with the probiotic [0] were of 5log10CFU/g, whereas those in the small intestine were of 5log10CFU/g in the animals inoculated with LAB and lactose, and approximately 1log10CFU/g less in the group inoculated with LAB. The levels of Salmonella in both, the small and large intestines, were between 3log10CFU/g and 4log10CFU/g. High microbial loads were found in the internal organs. Lactobacillus spp. were found only in the lymph nodes of the two groups inoculated with the probiotic, in very low amounts (<1log10CFU/g). Despite the high concentration of Salmonella administered to calves, the LAB inoculum of bovine origin was not capable of translocating to the internal organs in the extreme situations of intestinal imbalance generated by the pathogen.
Fil: Frizzo, Laureano Sebastian. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; Argentina
Fil: Soto, Lorena Paola. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; Argentina
Fil: Bertozzi, Ezequiel. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; Argentina
Fil: Zbrun, María Virginia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; Argentina
Fil: Signorini Porchietto, Marcelo Lisandro. Universidad Nacional del Litoral; Argentina. Instituto Nacional de Tecnología Agropecuaria Eea, Rafaela; Argentina
Fil: Sequeira, Gabriel Jorge. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; Argentina
Fil: Rodriguez Armesto, Roberto. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; Argentina
Fil: Rosmini, Marcelo Raul. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; Argentina
Materia
CALVES
LACTOBACILLUS
LACTOSE
PROBIOTIC
SALMONELLA DUBLIN CHALLENGE
TRANSLOCATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/96484
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/96484
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Intestinal populations of Lactobacilli and coliforms after in vivo Salmonella dublin challenge and their relationship with microbial translocation in calves supplemented with lactic acid bacteria and lactoseFrizzo, Laureano SebastianSoto, Lorena PaolaBertozzi, EzequielZbrun, María VirginiaSignorini Porchietto, Marcelo LisandroSequeira, Gabriel JorgeRodriguez Armesto, RobertoRosmini, Marcelo RaulCALVESLACTOBACILLUSLACTOSEPROBIOTICSALMONELLA DUBLIN CHALLENGETRANSLOCATIONhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The aim of the present study was to evaluate the relationship between intestinal microbial populations and bacterial translocation in young calves supplemented with lactic acid bacteria (LAB) and lactose and experimentally infected with Salmonella dublin DSPV 595T. Fifteen calves were divided into three groups (a control group, a group inoculated with LAB, and a group inoculated with LAB and lactose), with 5, 6 and 4 animals each, were used. The LAB inoculum, composed of Lactobacillus casei DSPV 318T, L. salivarius DSPV 315T and Pediococcus acidilactici DSPV 006T was administered together with the milk replacer. The groups inoculated with LAB and LAB and lactose received a daily dose of 109CFU/kg body weight of each strain throughout the experiment. Lactose was provided in doses of 100g/d. The administration of the pathogen was performed on all animals on day 11 of the experiment with 2×1010CFU. The levels of probiotic inoculum found in the large intestine of the animals from the two groups inoculated with the probiotic [0] were of 5log10CFU/g, whereas those in the small intestine were of 5log10CFU/g in the animals inoculated with LAB and lactose, and approximately 1log10CFU/g less in the group inoculated with LAB. The levels of Salmonella in both, the small and large intestines, were between 3log10CFU/g and 4log10CFU/g. High microbial loads were found in the internal organs. Lactobacillus spp. were found only in the lymph nodes of the two groups inoculated with the probiotic, in very low amounts (<1log10CFU/g). Despite the high concentration of Salmonella administered to calves, the LAB inoculum of bovine origin was not capable of translocating to the internal organs in the extreme situations of intestinal imbalance generated by the pathogen.Fil: Frizzo, Laureano Sebastian. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; ArgentinaFil: Soto, Lorena Paola. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; ArgentinaFil: Bertozzi, Ezequiel. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; ArgentinaFil: Zbrun, María Virginia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; ArgentinaFil: Signorini Porchietto, Marcelo Lisandro. Universidad Nacional del Litoral; Argentina. Instituto Nacional de Tecnología Agropecuaria Eea, Rafaela; ArgentinaFil: Sequeira, Gabriel Jorge. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; ArgentinaFil: Rodriguez Armesto, Roberto. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; ArgentinaFil: Rosmini, Marcelo Raul. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; ArgentinaElsevier Science2011-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96484Frizzo, Laureano Sebastian; Soto, Lorena Paola; Bertozzi, Ezequiel; Zbrun, María Virginia; Signorini Porchietto, Marcelo Lisandro; et al.; Intestinal populations of Lactobacilli and coliforms after in vivo Salmonella dublin challenge and their relationship with microbial translocation in calves supplemented with lactic acid bacteria and lactose; Elsevier Science; Animal Feed Science and Technology; 170; 1-2; 11-2011; 12-200377-8401CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/journal/03778401info:eu-repo/semantics/altIdentifier/doi/10.1016/j.anifeedsci.2011.07.016info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:22:07Zoai:ri.conicet.gov.ar:11336/96484instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:22:08.03CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Intestinal populations of Lactobacilli and coliforms after in vivo Salmonella dublin challenge and their relationship with microbial translocation in calves supplemented with lactic acid bacteria and lactose
title Intestinal populations of Lactobacilli and coliforms after in vivo Salmonella dublin challenge and their relationship with microbial translocation in calves supplemented with lactic acid bacteria and lactose
spellingShingle Intestinal populations of Lactobacilli and coliforms after in vivo Salmonella dublin challenge and their relationship with microbial translocation in calves supplemented with lactic acid bacteria and lactose
Frizzo, Laureano Sebastian
CALVES
LACTOBACILLUS
LACTOSE
PROBIOTIC
SALMONELLA DUBLIN CHALLENGE
TRANSLOCATION
title_short Intestinal populations of Lactobacilli and coliforms after in vivo Salmonella dublin challenge and their relationship with microbial translocation in calves supplemented with lactic acid bacteria and lactose
title_full Intestinal populations of Lactobacilli and coliforms after in vivo Salmonella dublin challenge and their relationship with microbial translocation in calves supplemented with lactic acid bacteria and lactose
title_fullStr Intestinal populations of Lactobacilli and coliforms after in vivo Salmonella dublin challenge and their relationship with microbial translocation in calves supplemented with lactic acid bacteria and lactose
title_full_unstemmed Intestinal populations of Lactobacilli and coliforms after in vivo Salmonella dublin challenge and their relationship with microbial translocation in calves supplemented with lactic acid bacteria and lactose
title_sort Intestinal populations of Lactobacilli and coliforms after in vivo Salmonella dublin challenge and their relationship with microbial translocation in calves supplemented with lactic acid bacteria and lactose
dc.creator.none.fl_str_mv Frizzo, Laureano Sebastian
Soto, Lorena Paola
Bertozzi, Ezequiel
Zbrun, María Virginia
Signorini Porchietto, Marcelo Lisandro
Sequeira, Gabriel Jorge
Rodriguez Armesto, Roberto
Rosmini, Marcelo Raul
author Frizzo, Laureano Sebastian
author_facet Frizzo, Laureano Sebastian
Soto, Lorena Paola
Bertozzi, Ezequiel
Zbrun, María Virginia
Signorini Porchietto, Marcelo Lisandro
Sequeira, Gabriel Jorge
Rodriguez Armesto, Roberto
Rosmini, Marcelo Raul
author_role author
author2 Soto, Lorena Paola
Bertozzi, Ezequiel
Zbrun, María Virginia
Signorini Porchietto, Marcelo Lisandro
Sequeira, Gabriel Jorge
Rodriguez Armesto, Roberto
Rosmini, Marcelo Raul
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CALVES
LACTOBACILLUS
LACTOSE
PROBIOTIC
SALMONELLA DUBLIN CHALLENGE
TRANSLOCATION
topic CALVES
LACTOBACILLUS
LACTOSE
PROBIOTIC
SALMONELLA DUBLIN CHALLENGE
TRANSLOCATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The aim of the present study was to evaluate the relationship between intestinal microbial populations and bacterial translocation in young calves supplemented with lactic acid bacteria (LAB) and lactose and experimentally infected with Salmonella dublin DSPV 595T. Fifteen calves were divided into three groups (a control group, a group inoculated with LAB, and a group inoculated with LAB and lactose), with 5, 6 and 4 animals each, were used. The LAB inoculum, composed of Lactobacillus casei DSPV 318T, L. salivarius DSPV 315T and Pediococcus acidilactici DSPV 006T was administered together with the milk replacer. The groups inoculated with LAB and LAB and lactose received a daily dose of 109CFU/kg body weight of each strain throughout the experiment. Lactose was provided in doses of 100g/d. The administration of the pathogen was performed on all animals on day 11 of the experiment with 2×1010CFU. The levels of probiotic inoculum found in the large intestine of the animals from the two groups inoculated with the probiotic [0] were of 5log10CFU/g, whereas those in the small intestine were of 5log10CFU/g in the animals inoculated with LAB and lactose, and approximately 1log10CFU/g less in the group inoculated with LAB. The levels of Salmonella in both, the small and large intestines, were between 3log10CFU/g and 4log10CFU/g. High microbial loads were found in the internal organs. Lactobacillus spp. were found only in the lymph nodes of the two groups inoculated with the probiotic, in very low amounts (<1log10CFU/g). Despite the high concentration of Salmonella administered to calves, the LAB inoculum of bovine origin was not capable of translocating to the internal organs in the extreme situations of intestinal imbalance generated by the pathogen.
Fil: Frizzo, Laureano Sebastian. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; Argentina
Fil: Soto, Lorena Paola. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; Argentina
Fil: Bertozzi, Ezequiel. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; Argentina
Fil: Zbrun, María Virginia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; Argentina
Fil: Signorini Porchietto, Marcelo Lisandro. Universidad Nacional del Litoral; Argentina. Instituto Nacional de Tecnología Agropecuaria Eea, Rafaela; Argentina
Fil: Sequeira, Gabriel Jorge. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; Argentina
Fil: Rodriguez Armesto, Roberto. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; Argentina
Fil: Rosmini, Marcelo Raul. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; Argentina
description The aim of the present study was to evaluate the relationship between intestinal microbial populations and bacterial translocation in young calves supplemented with lactic acid bacteria (LAB) and lactose and experimentally infected with Salmonella dublin DSPV 595T. Fifteen calves were divided into three groups (a control group, a group inoculated with LAB, and a group inoculated with LAB and lactose), with 5, 6 and 4 animals each, were used. The LAB inoculum, composed of Lactobacillus casei DSPV 318T, L. salivarius DSPV 315T and Pediococcus acidilactici DSPV 006T was administered together with the milk replacer. The groups inoculated with LAB and LAB and lactose received a daily dose of 109CFU/kg body weight of each strain throughout the experiment. Lactose was provided in doses of 100g/d. The administration of the pathogen was performed on all animals on day 11 of the experiment with 2×1010CFU. The levels of probiotic inoculum found in the large intestine of the animals from the two groups inoculated with the probiotic [0] were of 5log10CFU/g, whereas those in the small intestine were of 5log10CFU/g in the animals inoculated with LAB and lactose, and approximately 1log10CFU/g less in the group inoculated with LAB. The levels of Salmonella in both, the small and large intestines, were between 3log10CFU/g and 4log10CFU/g. High microbial loads were found in the internal organs. Lactobacillus spp. were found only in the lymph nodes of the two groups inoculated with the probiotic, in very low amounts (<1log10CFU/g). Despite the high concentration of Salmonella administered to calves, the LAB inoculum of bovine origin was not capable of translocating to the internal organs in the extreme situations of intestinal imbalance generated by the pathogen.
publishDate 2011
dc.date.none.fl_str_mv 2011-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/96484
Frizzo, Laureano Sebastian; Soto, Lorena Paola; Bertozzi, Ezequiel; Zbrun, María Virginia; Signorini Porchietto, Marcelo Lisandro; et al.; Intestinal populations of Lactobacilli and coliforms after in vivo Salmonella dublin challenge and their relationship with microbial translocation in calves supplemented with lactic acid bacteria and lactose; Elsevier Science; Animal Feed Science and Technology; 170; 1-2; 11-2011; 12-20
0377-8401
CONICET Digital
CONICET
url http://hdl.handle.net/11336/96484
identifier_str_mv Frizzo, Laureano Sebastian; Soto, Lorena Paola; Bertozzi, Ezequiel; Zbrun, María Virginia; Signorini Porchietto, Marcelo Lisandro; et al.; Intestinal populations of Lactobacilli and coliforms after in vivo Salmonella dublin challenge and their relationship with microbial translocation in calves supplemented with lactic acid bacteria and lactose; Elsevier Science; Animal Feed Science and Technology; 170; 1-2; 11-2011; 12-20
0377-8401
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/journal/03778401
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.anifeedsci.2011.07.016
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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