Comparative EvaluationIn vitroof the HerbicideFlurochloridone by Cytokinesis-blockMicronucleus Cytome and Comet Assays

Autores
Nikoloff, Noelia; Larramendy, Marcelo Luis; Soloneski, Sonia Maria Elsa
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Thein-vitroeffects of flurochloridone and its formulations Twin Pack Gold1(25% a.i.) andRainbow1(25% a.i.) were evaluated in Chinese Hamster Ovary K1 (CHO-K1) cells. The cytokinesis-blockmicronucleus cytome (CBMN-cyt) and single-cell gel electrophoresis (SCGE) assays were used. Theactivities were tested within the range of final concentrations of 0.25–15lg flurochloridone/mL. Theresults demonstrated that both the flurochloridone and Rainbow1were not able to induce micronuclei(MN). On the other hand, Twin Pack Gold1only increased the frequency of MN at 5lg/mL. Furthermore,10 and 15lg/mL of both formulations resulted in a cellular cytotoxicity demonstrated by alterations in thenuclear division index and cellular death. SCGE assay appeared to be a more sensitive bioassay fordetecting primary DNA strand breaks at lower concentrations of flurochloridone than MN did. A markedincrease in the genetic damage index was observed when 5 and 15lg/mL of both flurochloridone andRainbow1but only when 15lg/mL of Twin Pack Gold1were used. This is the first report demonstratingthat flurochloridone and its two commercial formulations are able to induce single-strand DNA breaksinvitroon mammalian cells.
Fil: Nikoloff, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: Larramendy, Marcelo Luis. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: Soloneski, Sonia Maria Elsa. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Materia
Flurochloridone
Cbmn-Cyt Assay
Dna-Single Strands Breaks
Cho-K1 Cells
Commercial Formulations
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/32872
Acceder
id CONICETDig_419ddecce43d1efb770d508998a5444c
oai_identifier_str oai:ri.conicet.gov.ar:11336/32872
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Comparative EvaluationIn vitroof the HerbicideFlurochloridone by Cytokinesis-blockMicronucleus Cytome and Comet AssaysNikoloff, NoeliaLarramendy, Marcelo LuisSoloneski, Sonia Maria ElsaFlurochloridoneCbmn-Cyt AssayDna-Single Strands BreaksCho-K1 CellsCommercial Formulationshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Thein-vitroeffects of flurochloridone and its formulations Twin Pack Gold1(25% a.i.) andRainbow1(25% a.i.) were evaluated in Chinese Hamster Ovary K1 (CHO-K1) cells. The cytokinesis-blockmicronucleus cytome (CBMN-cyt) and single-cell gel electrophoresis (SCGE) assays were used. Theactivities were tested within the range of final concentrations of 0.25–15lg flurochloridone/mL. Theresults demonstrated that both the flurochloridone and Rainbow1were not able to induce micronuclei(MN). On the other hand, Twin Pack Gold1only increased the frequency of MN at 5lg/mL. Furthermore,10 and 15lg/mL of both formulations resulted in a cellular cytotoxicity demonstrated by alterations in thenuclear division index and cellular death. SCGE assay appeared to be a more sensitive bioassay fordetecting primary DNA strand breaks at lower concentrations of flurochloridone than MN did. A markedincrease in the genetic damage index was observed when 5 and 15lg/mL of both flurochloridone andRainbow1but only when 15lg/mL of Twin Pack Gold1were used. This is the first report demonstratingthat flurochloridone and its two commercial formulations are able to induce single-strand DNA breaksinvitroon mammalian cells.Fil: Nikoloff, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; ArgentinaFil: Larramendy, Marcelo Luis. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; ArgentinaFil: Soloneski, Sonia Maria Elsa. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; ArgentinaJohn Wiley & Sons Inc2014-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32872Nikoloff, Noelia; Larramendy, Marcelo Luis; Soloneski, Sonia Maria Elsa; Comparative EvaluationIn vitroof the HerbicideFlurochloridone by Cytokinesis-blockMicronucleus Cytome and Comet Assays; John Wiley & Sons Inc; Environmental Toxicology; 29; 8; 10-2014; 884-8921520-4081CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/tox.21816info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/tox.21816/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:39:51Zoai:ri.conicet.gov.ar:11336/32872instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:39:52.007CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparative EvaluationIn vitroof the HerbicideFlurochloridone by Cytokinesis-blockMicronucleus Cytome and Comet Assays
title Comparative EvaluationIn vitroof the HerbicideFlurochloridone by Cytokinesis-blockMicronucleus Cytome and Comet Assays
spellingShingle Comparative EvaluationIn vitroof the HerbicideFlurochloridone by Cytokinesis-blockMicronucleus Cytome and Comet Assays
Nikoloff, Noelia
Flurochloridone
Cbmn-Cyt Assay
Dna-Single Strands Breaks
Cho-K1 Cells
Commercial Formulations
title_short Comparative EvaluationIn vitroof the HerbicideFlurochloridone by Cytokinesis-blockMicronucleus Cytome and Comet Assays
title_full Comparative EvaluationIn vitroof the HerbicideFlurochloridone by Cytokinesis-blockMicronucleus Cytome and Comet Assays
title_fullStr Comparative EvaluationIn vitroof the HerbicideFlurochloridone by Cytokinesis-blockMicronucleus Cytome and Comet Assays
title_full_unstemmed Comparative EvaluationIn vitroof the HerbicideFlurochloridone by Cytokinesis-blockMicronucleus Cytome and Comet Assays
title_sort Comparative EvaluationIn vitroof the HerbicideFlurochloridone by Cytokinesis-blockMicronucleus Cytome and Comet Assays
dc.creator.none.fl_str_mv Nikoloff, Noelia
Larramendy, Marcelo Luis
Soloneski, Sonia Maria Elsa
author Nikoloff, Noelia
author_facet Nikoloff, Noelia
Larramendy, Marcelo Luis
Soloneski, Sonia Maria Elsa
author_role author
author2 Larramendy, Marcelo Luis
Soloneski, Sonia Maria Elsa
author2_role author
author
dc.subject.none.fl_str_mv Flurochloridone
Cbmn-Cyt Assay
Dna-Single Strands Breaks
Cho-K1 Cells
Commercial Formulations
topic Flurochloridone
Cbmn-Cyt Assay
Dna-Single Strands Breaks
Cho-K1 Cells
Commercial Formulations
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Thein-vitroeffects of flurochloridone and its formulations Twin Pack Gold1(25% a.i.) andRainbow1(25% a.i.) were evaluated in Chinese Hamster Ovary K1 (CHO-K1) cells. The cytokinesis-blockmicronucleus cytome (CBMN-cyt) and single-cell gel electrophoresis (SCGE) assays were used. Theactivities were tested within the range of final concentrations of 0.25–15lg flurochloridone/mL. Theresults demonstrated that both the flurochloridone and Rainbow1were not able to induce micronuclei(MN). On the other hand, Twin Pack Gold1only increased the frequency of MN at 5lg/mL. Furthermore,10 and 15lg/mL of both formulations resulted in a cellular cytotoxicity demonstrated by alterations in thenuclear division index and cellular death. SCGE assay appeared to be a more sensitive bioassay fordetecting primary DNA strand breaks at lower concentrations of flurochloridone than MN did. A markedincrease in the genetic damage index was observed when 5 and 15lg/mL of both flurochloridone andRainbow1but only when 15lg/mL of Twin Pack Gold1were used. This is the first report demonstratingthat flurochloridone and its two commercial formulations are able to induce single-strand DNA breaksinvitroon mammalian cells.
Fil: Nikoloff, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: Larramendy, Marcelo Luis. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: Soloneski, Sonia Maria Elsa. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
description Thein-vitroeffects of flurochloridone and its formulations Twin Pack Gold1(25% a.i.) andRainbow1(25% a.i.) were evaluated in Chinese Hamster Ovary K1 (CHO-K1) cells. The cytokinesis-blockmicronucleus cytome (CBMN-cyt) and single-cell gel electrophoresis (SCGE) assays were used. Theactivities were tested within the range of final concentrations of 0.25–15lg flurochloridone/mL. Theresults demonstrated that both the flurochloridone and Rainbow1were not able to induce micronuclei(MN). On the other hand, Twin Pack Gold1only increased the frequency of MN at 5lg/mL. Furthermore,10 and 15lg/mL of both formulations resulted in a cellular cytotoxicity demonstrated by alterations in thenuclear division index and cellular death. SCGE assay appeared to be a more sensitive bioassay fordetecting primary DNA strand breaks at lower concentrations of flurochloridone than MN did. A markedincrease in the genetic damage index was observed when 5 and 15lg/mL of both flurochloridone andRainbow1but only when 15lg/mL of Twin Pack Gold1were used. This is the first report demonstratingthat flurochloridone and its two commercial formulations are able to induce single-strand DNA breaksinvitroon mammalian cells.
publishDate 2014
dc.date.none.fl_str_mv 2014-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/32872
Nikoloff, Noelia; Larramendy, Marcelo Luis; Soloneski, Sonia Maria Elsa; Comparative EvaluationIn vitroof the HerbicideFlurochloridone by Cytokinesis-blockMicronucleus Cytome and Comet Assays; John Wiley & Sons Inc; Environmental Toxicology; 29; 8; 10-2014; 884-892
1520-4081
CONICET Digital
CONICET
url http://hdl.handle.net/11336/32872
identifier_str_mv Nikoloff, Noelia; Larramendy, Marcelo Luis; Soloneski, Sonia Maria Elsa; Comparative EvaluationIn vitroof the HerbicideFlurochloridone by Cytokinesis-blockMicronucleus Cytome and Comet Assays; John Wiley & Sons Inc; Environmental Toxicology; 29; 8; 10-2014; 884-892
1520-4081
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/tox.21816
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/tox.21816/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Inc
publisher.none.fl_str_mv John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613260898205696
score 13.070432

Publicaciones similares