Enzymes involved in the biosynthesis of sphingolipids with very-long-chain PUFA concur with male germ cell differentiation

Autores
Oresti, Gerardo Martin; Santiago Valtierra, Florencia Ximena; Reyes, Juan; Aveldaño, Marta Isabel
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The sphingolipids sphingomyelin (SM) and ceramide (Cer), of male rodent germ cells (spermatocytes, spermatids) and spermatozoa contain very-long-chain (C28-C32) polyenoic fatty acids (VLCPUFA) in nonhydroxy (n-V) and 2-hydroxy forms (h-V) not present in Sertoli cells. During postnatal development, SM and Cer species with n-V appear in testes concomitantly with pachytene spermatocytes and those with h-V with spermatids. Both are able to biosynthesize their own sphingolipids, the former more actively than the latter. The biosynthesis of n-V requires elongation of PUFA (20:4n-6, 22:5n-6) by elongation of very-long-chain fatty acid (Elovl) proteins, while that of h-V requires a fatty acid 2-hydroxylase (Fa2h). Elovl5 and Elovl2, coding for enzymes responsible for PUFA biosynthesis, and Elovl4, in turn responsible for the formation of PUFA longer than C26, are actively expressed in germ cells. The Elovl4 protein is exclusively expressed in germ cells in a stage-dependent manner, spermatocytes displaying the highest Elovl4 protein levels and enzymatic activity. Along with high proportion of h-V Cer and h-V SM species, the Fa2h protein is mainly concentrated in late spermatids, in the step of spermiogenesis in which they elongate and their heads change shape. Consistently, spermatocytes express the highest levels of ceramide synthase 3, required for the N-acylation of sphingosine with a VLCPUFA, while spermatids express the highest levels of SM synthase 2. Irrespective of the maturation stage, the small raftlike domains of the germ cell plasma membrane contain species of SM and Cer with saturated fatty acids, while those containing n-V and h-V abound in the large non-raft areas. These species, with unique physico-chemical properties, could play a structural role facilitating the germ cell shape changes associated with the progress of spermatogenesis. If released from such species, free n-V and h-V could be involved in germ cell differentiation as potential precursors of uncommon elovanoid-like bioactive derivatives.
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Santiago Valtierra, Florencia Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Reyes, Juan. Pontificia Universidad Católica de Valparaíso; Chile
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Congreso Latinoamericano Conjunto de Reproducción
Santiago
Chile
Sociedad Chilena de Reproducción y Desarrollo
Materia
SPHINGOLIPID SYNTHASES
FATTY ACID ELONGASES
DE NOVO BIOSYNTHESIS
SPERMATOGENIC CELLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/223466
Acceder
id CONICETDig_89830f7bebcce9f0e3be14598cbdf7c9
oai_identifier_str oai:ri.conicet.gov.ar:11336/223466
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Enzymes involved in the biosynthesis of sphingolipids with very-long-chain PUFA concur with male germ cell differentiationOresti, Gerardo MartinSantiago Valtierra, Florencia XimenaReyes, JuanAveldaño, Marta IsabelSPHINGOLIPID SYNTHASESFATTY ACID ELONGASESDE NOVO BIOSYNTHESISSPERMATOGENIC CELLShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The sphingolipids sphingomyelin (SM) and ceramide (Cer), of male rodent germ cells (spermatocytes, spermatids) and spermatozoa contain very-long-chain (C28-C32) polyenoic fatty acids (VLCPUFA) in nonhydroxy (n-V) and 2-hydroxy forms (h-V) not present in Sertoli cells. During postnatal development, SM and Cer species with n-V appear in testes concomitantly with pachytene spermatocytes and those with h-V with spermatids. Both are able to biosynthesize their own sphingolipids, the former more actively than the latter. The biosynthesis of n-V requires elongation of PUFA (20:4n-6, 22:5n-6) by elongation of very-long-chain fatty acid (Elovl) proteins, while that of h-V requires a fatty acid 2-hydroxylase (Fa2h). Elovl5 and Elovl2, coding for enzymes responsible for PUFA biosynthesis, and Elovl4, in turn responsible for the formation of PUFA longer than C26, are actively expressed in germ cells. The Elovl4 protein is exclusively expressed in germ cells in a stage-dependent manner, spermatocytes displaying the highest Elovl4 protein levels and enzymatic activity. Along with high proportion of h-V Cer and h-V SM species, the Fa2h protein is mainly concentrated in late spermatids, in the step of spermiogenesis in which they elongate and their heads change shape. Consistently, spermatocytes express the highest levels of ceramide synthase 3, required for the N-acylation of sphingosine with a VLCPUFA, while spermatids express the highest levels of SM synthase 2. Irrespective of the maturation stage, the small raftlike domains of the germ cell plasma membrane contain species of SM and Cer with saturated fatty acids, while those containing n-V and h-V abound in the large non-raft areas. These species, with unique physico-chemical properties, could play a structural role facilitating the germ cell shape changes associated with the progress of spermatogenesis. If released from such species, free n-V and h-V could be involved in germ cell differentiation as potential precursors of uncommon elovanoid-like bioactive derivatives.Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Santiago Valtierra, Florencia Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Reyes, Juan. Pontificia Universidad Católica de Valparaíso; ChileFil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaCongreso Latinoamericano Conjunto de ReproducciónSantiagoChileSociedad Chilena de Reproducción y DesarrolloUniversidad de Valparaíso2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/223466Enzymes involved in the biosynthesis of sphingolipids with very-long-chain PUFA concur with male germ cell differentiation; Congreso Latinoamericano Conjunto de Reproducción; Santiago; Chile; 2019; 2-3CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://schrd.cl/wp-content/uploads/2021/03/LIBRO-DE-RESUMENES-CONGRESO-CONJUNTO-REPRODUCCION-2019.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:03:59Zoai:ri.conicet.gov.ar:11336/223466instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:03:59.283CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Enzymes involved in the biosynthesis of sphingolipids with very-long-chain PUFA concur with male germ cell differentiation
title Enzymes involved in the biosynthesis of sphingolipids with very-long-chain PUFA concur with male germ cell differentiation
spellingShingle Enzymes involved in the biosynthesis of sphingolipids with very-long-chain PUFA concur with male germ cell differentiation
Oresti, Gerardo Martin
SPHINGOLIPID SYNTHASES
FATTY ACID ELONGASES
DE NOVO BIOSYNTHESIS
SPERMATOGENIC CELLS
title_short Enzymes involved in the biosynthesis of sphingolipids with very-long-chain PUFA concur with male germ cell differentiation
title_full Enzymes involved in the biosynthesis of sphingolipids with very-long-chain PUFA concur with male germ cell differentiation
title_fullStr Enzymes involved in the biosynthesis of sphingolipids with very-long-chain PUFA concur with male germ cell differentiation
title_full_unstemmed Enzymes involved in the biosynthesis of sphingolipids with very-long-chain PUFA concur with male germ cell differentiation
title_sort Enzymes involved in the biosynthesis of sphingolipids with very-long-chain PUFA concur with male germ cell differentiation
dc.creator.none.fl_str_mv Oresti, Gerardo Martin
Santiago Valtierra, Florencia Ximena
Reyes, Juan
Aveldaño, Marta Isabel
author Oresti, Gerardo Martin
author_facet Oresti, Gerardo Martin
Santiago Valtierra, Florencia Ximena
Reyes, Juan
Aveldaño, Marta Isabel
author_role author
author2 Santiago Valtierra, Florencia Ximena
Reyes, Juan
Aveldaño, Marta Isabel
author2_role author
author
author
dc.subject.none.fl_str_mv SPHINGOLIPID SYNTHASES
FATTY ACID ELONGASES
DE NOVO BIOSYNTHESIS
SPERMATOGENIC CELLS
topic SPHINGOLIPID SYNTHASES
FATTY ACID ELONGASES
DE NOVO BIOSYNTHESIS
SPERMATOGENIC CELLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The sphingolipids sphingomyelin (SM) and ceramide (Cer), of male rodent germ cells (spermatocytes, spermatids) and spermatozoa contain very-long-chain (C28-C32) polyenoic fatty acids (VLCPUFA) in nonhydroxy (n-V) and 2-hydroxy forms (h-V) not present in Sertoli cells. During postnatal development, SM and Cer species with n-V appear in testes concomitantly with pachytene spermatocytes and those with h-V with spermatids. Both are able to biosynthesize their own sphingolipids, the former more actively than the latter. The biosynthesis of n-V requires elongation of PUFA (20:4n-6, 22:5n-6) by elongation of very-long-chain fatty acid (Elovl) proteins, while that of h-V requires a fatty acid 2-hydroxylase (Fa2h). Elovl5 and Elovl2, coding for enzymes responsible for PUFA biosynthesis, and Elovl4, in turn responsible for the formation of PUFA longer than C26, are actively expressed in germ cells. The Elovl4 protein is exclusively expressed in germ cells in a stage-dependent manner, spermatocytes displaying the highest Elovl4 protein levels and enzymatic activity. Along with high proportion of h-V Cer and h-V SM species, the Fa2h protein is mainly concentrated in late spermatids, in the step of spermiogenesis in which they elongate and their heads change shape. Consistently, spermatocytes express the highest levels of ceramide synthase 3, required for the N-acylation of sphingosine with a VLCPUFA, while spermatids express the highest levels of SM synthase 2. Irrespective of the maturation stage, the small raftlike domains of the germ cell plasma membrane contain species of SM and Cer with saturated fatty acids, while those containing n-V and h-V abound in the large non-raft areas. These species, with unique physico-chemical properties, could play a structural role facilitating the germ cell shape changes associated with the progress of spermatogenesis. If released from such species, free n-V and h-V could be involved in germ cell differentiation as potential precursors of uncommon elovanoid-like bioactive derivatives.
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Santiago Valtierra, Florencia Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Reyes, Juan. Pontificia Universidad Católica de Valparaíso; Chile
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Congreso Latinoamericano Conjunto de Reproducción
Santiago
Chile
Sociedad Chilena de Reproducción y Desarrollo
description The sphingolipids sphingomyelin (SM) and ceramide (Cer), of male rodent germ cells (spermatocytes, spermatids) and spermatozoa contain very-long-chain (C28-C32) polyenoic fatty acids (VLCPUFA) in nonhydroxy (n-V) and 2-hydroxy forms (h-V) not present in Sertoli cells. During postnatal development, SM and Cer species with n-V appear in testes concomitantly with pachytene spermatocytes and those with h-V with spermatids. Both are able to biosynthesize their own sphingolipids, the former more actively than the latter. The biosynthesis of n-V requires elongation of PUFA (20:4n-6, 22:5n-6) by elongation of very-long-chain fatty acid (Elovl) proteins, while that of h-V requires a fatty acid 2-hydroxylase (Fa2h). Elovl5 and Elovl2, coding for enzymes responsible for PUFA biosynthesis, and Elovl4, in turn responsible for the formation of PUFA longer than C26, are actively expressed in germ cells. The Elovl4 protein is exclusively expressed in germ cells in a stage-dependent manner, spermatocytes displaying the highest Elovl4 protein levels and enzymatic activity. Along with high proportion of h-V Cer and h-V SM species, the Fa2h protein is mainly concentrated in late spermatids, in the step of spermiogenesis in which they elongate and their heads change shape. Consistently, spermatocytes express the highest levels of ceramide synthase 3, required for the N-acylation of sphingosine with a VLCPUFA, while spermatids express the highest levels of SM synthase 2. Irrespective of the maturation stage, the small raftlike domains of the germ cell plasma membrane contain species of SM and Cer with saturated fatty acids, while those containing n-V and h-V abound in the large non-raft areas. These species, with unique physico-chemical properties, could play a structural role facilitating the germ cell shape changes associated with the progress of spermatogenesis. If released from such species, free n-V and h-V could be involved in germ cell differentiation as potential precursors of uncommon elovanoid-like bioactive derivatives.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/223466
Enzymes involved in the biosynthesis of sphingolipids with very-long-chain PUFA concur with male germ cell differentiation; Congreso Latinoamericano Conjunto de Reproducción; Santiago; Chile; 2019; 2-3
CONICET Digital
CONICET
url http://hdl.handle.net/11336/223466
identifier_str_mv Enzymes involved in the biosynthesis of sphingolipids with very-long-chain PUFA concur with male germ cell differentiation; Congreso Latinoamericano Conjunto de Reproducción; Santiago; Chile; 2019; 2-3
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://schrd.cl/wp-content/uploads/2021/03/LIBRO-DE-RESUMENES-CONGRESO-CONJUNTO-REPRODUCCION-2019.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Universidad de Valparaíso
publisher.none.fl_str_mv Universidad de Valparaíso
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980120512430080
score 12.993085

Publicaciones similares