Homeobox-Clock protein interaction in zebrafish: a shared mechanism for pineal-specific and circadian gene expression

Autores
Appelbaum, Lior; Anzulovich Miranda, Ana Cecilia; Baler, Ruben; Gothilf, Yoav
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In non-mammalian vertebrates, the pineal gland is photoreceptive and contains an intrinsic circadian oscillator that drives rhythmic production and secretion of melatonin. These features require an accurate spatiotemporal expression of an array of specific genes in the pineal gland. Among these is the arylalkylamine N-acetyltransferase, a key enzyme in the melatonin production pathway. In zebrafish, pineal specificity of zfaanat2 is determined by a region designated the pineal-restrictive downstream module (PRDM), which contains three photoreceptor conserved elements (PCEs) and an E-box, elements that are generally associated with photoreceptor-specific and rhythmic expression, respectively. Here, by using in vivo and in vitro approaches, it was found that the PCEs and E-box of the PRDM mediate a synergistic effect of the photoreceptor-specific homeobox OTX5 and rhythmically expressed clock protein heterodimer, BMAL/CLOCK, on zfaanat2 expression. Furthermore, the distance between the PCEs and the E-box was found to be critical for PRDM function, suggesting a possible physical feature of this synergistic interaction. OTX5-BMAL/CLOCK may act through this mechanism to simultaneously control pineal-specific and rhythmic expression of zfaanat2 and possibly also other pineal and retinal genes.
Fil: Appelbaum, Lior. Bar-Ilan University. Faculty of Life Sciences; Israel. Universitat Tel Aviv; Israel
Fil: Anzulovich Miranda, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. National Institutes of Health; Estados Unidos
Fil: Baler, Ruben. National Institutes of Health; Estados Unidos
Fil: Gothilf, Yoav. Universitat Tel Aviv; Israel
Materia
NON-MAMMALIAN VERRTEBRATE
PINEAL GLAND
A-NAT
CIRCADIAN RHYTHM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/158902
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/158902
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Homeobox-Clock protein interaction in zebrafish: a shared mechanism for pineal-specific and circadian gene expressionAppelbaum, LiorAnzulovich Miranda, Ana CeciliaBaler, RubenGothilf, YoavNON-MAMMALIAN VERRTEBRATEPINEAL GLANDA-NATCIRCADIAN RHYTHMhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In non-mammalian vertebrates, the pineal gland is photoreceptive and contains an intrinsic circadian oscillator that drives rhythmic production and secretion of melatonin. These features require an accurate spatiotemporal expression of an array of specific genes in the pineal gland. Among these is the arylalkylamine N-acetyltransferase, a key enzyme in the melatonin production pathway. In zebrafish, pineal specificity of zfaanat2 is determined by a region designated the pineal-restrictive downstream module (PRDM), which contains three photoreceptor conserved elements (PCEs) and an E-box, elements that are generally associated with photoreceptor-specific and rhythmic expression, respectively. Here, by using in vivo and in vitro approaches, it was found that the PCEs and E-box of the PRDM mediate a synergistic effect of the photoreceptor-specific homeobox OTX5 and rhythmically expressed clock protein heterodimer, BMAL/CLOCK, on zfaanat2 expression. Furthermore, the distance between the PCEs and the E-box was found to be critical for PRDM function, suggesting a possible physical feature of this synergistic interaction. OTX5-BMAL/CLOCK may act through this mechanism to simultaneously control pineal-specific and rhythmic expression of zfaanat2 and possibly also other pineal and retinal genes.Fil: Appelbaum, Lior. Bar-Ilan University. Faculty of Life Sciences; Israel. Universitat Tel Aviv; IsraelFil: Anzulovich Miranda, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. National Institutes of Health; Estados UnidosFil: Baler, Ruben. National Institutes of Health; Estados UnidosFil: Gothilf, Yoav. Universitat Tel Aviv; IsraelAmerican Society for Biochemistry and Molecular Biology2005-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/158902Appelbaum, Lior; Anzulovich Miranda, Ana Cecilia; Baler, Ruben; Gothilf, Yoav; Homeobox-Clock protein interaction in zebrafish: a shared mechanism for pineal-specific and circadian gene expression; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry; 280; 12; 3-2005; 11544-115510021-9258CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.jbc.org/article/S0021-9258(20)80859-X/fulltextinfo:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.M412935200info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:05:52Zoai:ri.conicet.gov.ar:11336/158902instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:05:52.799CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Homeobox-Clock protein interaction in zebrafish: a shared mechanism for pineal-specific and circadian gene expression
title Homeobox-Clock protein interaction in zebrafish: a shared mechanism for pineal-specific and circadian gene expression
spellingShingle Homeobox-Clock protein interaction in zebrafish: a shared mechanism for pineal-specific and circadian gene expression
Appelbaum, Lior
NON-MAMMALIAN VERRTEBRATE
PINEAL GLAND
A-NAT
CIRCADIAN RHYTHM
title_short Homeobox-Clock protein interaction in zebrafish: a shared mechanism for pineal-specific and circadian gene expression
title_full Homeobox-Clock protein interaction in zebrafish: a shared mechanism for pineal-specific and circadian gene expression
title_fullStr Homeobox-Clock protein interaction in zebrafish: a shared mechanism for pineal-specific and circadian gene expression
title_full_unstemmed Homeobox-Clock protein interaction in zebrafish: a shared mechanism for pineal-specific and circadian gene expression
title_sort Homeobox-Clock protein interaction in zebrafish: a shared mechanism for pineal-specific and circadian gene expression
dc.creator.none.fl_str_mv Appelbaum, Lior
Anzulovich Miranda, Ana Cecilia
Baler, Ruben
Gothilf, Yoav
author Appelbaum, Lior
author_facet Appelbaum, Lior
Anzulovich Miranda, Ana Cecilia
Baler, Ruben
Gothilf, Yoav
author_role author
author2 Anzulovich Miranda, Ana Cecilia
Baler, Ruben
Gothilf, Yoav
author2_role author
author
author
dc.subject.none.fl_str_mv NON-MAMMALIAN VERRTEBRATE
PINEAL GLAND
A-NAT
CIRCADIAN RHYTHM
topic NON-MAMMALIAN VERRTEBRATE
PINEAL GLAND
A-NAT
CIRCADIAN RHYTHM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In non-mammalian vertebrates, the pineal gland is photoreceptive and contains an intrinsic circadian oscillator that drives rhythmic production and secretion of melatonin. These features require an accurate spatiotemporal expression of an array of specific genes in the pineal gland. Among these is the arylalkylamine N-acetyltransferase, a key enzyme in the melatonin production pathway. In zebrafish, pineal specificity of zfaanat2 is determined by a region designated the pineal-restrictive downstream module (PRDM), which contains three photoreceptor conserved elements (PCEs) and an E-box, elements that are generally associated with photoreceptor-specific and rhythmic expression, respectively. Here, by using in vivo and in vitro approaches, it was found that the PCEs and E-box of the PRDM mediate a synergistic effect of the photoreceptor-specific homeobox OTX5 and rhythmically expressed clock protein heterodimer, BMAL/CLOCK, on zfaanat2 expression. Furthermore, the distance between the PCEs and the E-box was found to be critical for PRDM function, suggesting a possible physical feature of this synergistic interaction. OTX5-BMAL/CLOCK may act through this mechanism to simultaneously control pineal-specific and rhythmic expression of zfaanat2 and possibly also other pineal and retinal genes.
Fil: Appelbaum, Lior. Bar-Ilan University. Faculty of Life Sciences; Israel. Universitat Tel Aviv; Israel
Fil: Anzulovich Miranda, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. National Institutes of Health; Estados Unidos
Fil: Baler, Ruben. National Institutes of Health; Estados Unidos
Fil: Gothilf, Yoav. Universitat Tel Aviv; Israel
description In non-mammalian vertebrates, the pineal gland is photoreceptive and contains an intrinsic circadian oscillator that drives rhythmic production and secretion of melatonin. These features require an accurate spatiotemporal expression of an array of specific genes in the pineal gland. Among these is the arylalkylamine N-acetyltransferase, a key enzyme in the melatonin production pathway. In zebrafish, pineal specificity of zfaanat2 is determined by a region designated the pineal-restrictive downstream module (PRDM), which contains three photoreceptor conserved elements (PCEs) and an E-box, elements that are generally associated with photoreceptor-specific and rhythmic expression, respectively. Here, by using in vivo and in vitro approaches, it was found that the PCEs and E-box of the PRDM mediate a synergistic effect of the photoreceptor-specific homeobox OTX5 and rhythmically expressed clock protein heterodimer, BMAL/CLOCK, on zfaanat2 expression. Furthermore, the distance between the PCEs and the E-box was found to be critical for PRDM function, suggesting a possible physical feature of this synergistic interaction. OTX5-BMAL/CLOCK may act through this mechanism to simultaneously control pineal-specific and rhythmic expression of zfaanat2 and possibly also other pineal and retinal genes.
publishDate 2005
dc.date.none.fl_str_mv 2005-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/158902
Appelbaum, Lior; Anzulovich Miranda, Ana Cecilia; Baler, Ruben; Gothilf, Yoav; Homeobox-Clock protein interaction in zebrafish: a shared mechanism for pineal-specific and circadian gene expression; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry; 280; 12; 3-2005; 11544-11551
0021-9258
CONICET Digital
CONICET
url http://hdl.handle.net/11336/158902
identifier_str_mv Appelbaum, Lior; Anzulovich Miranda, Ana Cecilia; Baler, Ruben; Gothilf, Yoav; Homeobox-Clock protein interaction in zebrafish: a shared mechanism for pineal-specific and circadian gene expression; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry; 280; 12; 3-2005; 11544-11551
0021-9258
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.jbc.org/article/S0021-9258(20)80859-X/fulltext
info:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.M412935200
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980228738056192
score 12.993085

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